Click here to switch to the map view.

The map label for this gene is metG

Identifier: 78067237

GI number: 78067237

Start: 2879809

End: 2881971

Strand: Direct

Name: metG

Synonym: Bcep18194_A5768

Alternate gene names: 78067237

Gene position: 2879809-2881971 (Clockwise)

Preceding gene: 78067236

Following gene: 78067240

Centisome position: 77.96

GC content: 64.91

Gene sequence:

>2163_bases
ATGTCCGCATCCGACCTCACTTCCGTGCAGGCTGCGGCGCCGCAAGGTGACCGCCAGATCCTCGTTACGTCCGCACTGCC
CTATGCCAACGGGCAGATCCACATCGGCCACCTGGTCGAGTACATCCAGACCGACATCTGGGTGCGGACGCTGCGAATGC
ATGGCCATGAGGTCTACTACATCGGCGCCGACGATACGCACGGCACGCCGATCATGCTGCGCGCGGAGAAGGAAGGCCTG
ACCCCGAAGCAGCTGATCGATCGCGTGTGGACCGAACACAAGCGCGATTTCGACAGCTTCGGCGTGTCGTTCGACAACTT
CTACTCGACCGATTCGGACGAGAACCGCGTGCTGAGCGAGAAGATCTACGTCGCGCTGCAGGACGCCGGCTTCATCGCCG
AGCGCGAGATCGAGCAGGCCTACGATCCCGTCAAGGAAATGTTCTTGCCGGATCGCTTCATCAAGGGCGAGTGCCCGAAG
TGCCACGCGAAGGACCAGTACGGCGACAACTGCGAAGTGTGCGGCTCGACCTACCTGCCGACCGAACTGCTGAACCCGTA
TTCGGTCGTGTCGGGCGCGACGCCGGTGCGCAAGACGTCGAAGCACTACTTCTTCCGCCTGTCCGATCCGCGCTGCGAAA
CGTTCCTGCGCGAATGGGTGAGCGGCCTCGCGCAGCCCGAAGCCACCAACAAGATGCGCGAATGGCTCGGCGACGCCGGC
GAGGCCAAGCTCGCCGACTGGGACATCTCGCGTGACGCGCCGTACTTCGGCTTCGAGATCCCGGGCGCGCCCGGCAAGTA
TTTCTACGTGTGGCTCGACGCGCCGGTCGGCTACTACGCGAGCTTCAAGAACCTGTGCGACCGCAACGGCGTCGACTTCG
ATGCGTGGGTCCGCCCGGGCTCGACCACCGAGCAGTATCACTTCATCGGCAAGGACATCCTGTACTTCCACACGCTGTTC
TGGCCCGCGATGCTCGAGTTCTCGGGCCACCGCACGCCGACCAACGTGTTCGCGCACGGGTTCCTGACGGTCGACGGCGC
GAAGATGTCGAAGTCGCGCGGCACGTTCATCACCGCGCAGAGCTACATCGACACGGGCCTGAACCCCGAATGGCTGCGCT
ACTACTTCGCCGCGAAGCTGAACGCGACGATGGAAGACATCGACCTGAACCTCGACGACTTCCAGGCGCGCGTGAACAGC
GACCTCGTCGGCAAGTACGTGAACATCGCGAGCCGCGCGGCAGGCTTCCTGATCAAGCGCTTCGACGGCCGCGTGCAGGA
CAGCGCGATGAACCATCCGCTCGTCGCGAAGCTGCGCGAGGCGATCCCGCAGATCGCCGCGCACTACGAAGCACGCGAGT
ACGGCCGCGCACTGCGCCACACGATGGAGCTCGCGGACGAAGTGAACGCGTACGTCGATGGCGCCAAGCCGTGGGATCTC
GCCAAGGATCCGGCCAACGCGGTCGCGCTGCACGAAACCTGCAGCGTGAGCCTCGAGTCGTTCCGCCTGCTGTCGCTCGC
GCTGAAGCCGGTGATGCCGCGCGTGGCCGAAGCCGTCGAATCGTTCTTCGGGATCGCGCCGCTCGCCTGGTCCGATGCCG
CGAAGCCGCTGTCGTCGGAGCAACCGATCAAGGCGTACCAGCACCTGATGACGCGTGTCGACCCGAAGCAGATCGAAGCG
CTGCTCGCCGCGAACCGCGATTCGCTGCAAGCCGAAGCCACCGGCGCGGCTGTCGCGACCGCGAACGCCGCGAAGGATGC
GAAGAACAGCGCGAAGGCGAACGCAAACGCGAAGCAGGCTGTCGTGAACGGTGCGGACGACGCACCGATCTCGATCGACG
ATTTCGCGAAGATCGACCTGCGCATCGCGAAGATCGTCGCGTGCCAGGCCGTCGAAGGCTCGGACAAGCTGCTGCAGCTC
ACGCTCGACGTCGGCGAGGAAAAGACCCGCAACGTGTTCTCGGGCATCAAGTCCGCGTACCAGCCCGAGCAGCTCGTCGG
CAAGCTGACCGTGATGGTCGCGAACCTCGCGCCGCGCAAGATGAAGTTCGGCCTGTCCGAAGGGATGGTGCTCGCCGCGT
CGGCCACCGACGAGAAGGCCGAGCCGGGCCTCTATATCCTCGAGCCGCACAGCGGCGCGAAGCCCGGCATGCGCGTGAAG
TAA

Upstream 100 bases:

>100_bases
TTTCGGCGCCCGGTCAAGGGCGCCTGCCCGCGTCGCTGGCGCGCGCCCCTGCTAGAATCGCAGTTTCCCGTCGTTTTTCC
GCCGTTCCTCTACAGACCCT

Downstream 100 bases:

>100_bases
GCCGAACGCGCTGCAGCTTGCGCGAAAAAAAAGCCCCGCACATCGTGCGGGGCTTTTTTCATGGCTGCTCGGCGCGCCGC
TCGCGCGCGGCGTCGCTGCC

Product: methionyl-tRNA synthetase

Products: NA

Alternate protein names: Methionine--tRNA ligase; MetRS [H]

Number of amino acids: Translated: 720; Mature: 719

Protein sequence:

>720_residues
MSASDLTSVQAAAPQGDRQILVTSALPYANGQIHIGHLVEYIQTDIWVRTLRMHGHEVYYIGADDTHGTPIMLRAEKEGL
TPKQLIDRVWTEHKRDFDSFGVSFDNFYSTDSDENRVLSEKIYVALQDAGFIAEREIEQAYDPVKEMFLPDRFIKGECPK
CHAKDQYGDNCEVCGSTYLPTELLNPYSVVSGATPVRKTSKHYFFRLSDPRCETFLREWVSGLAQPEATNKMREWLGDAG
EAKLADWDISRDAPYFGFEIPGAPGKYFYVWLDAPVGYYASFKNLCDRNGVDFDAWVRPGSTTEQYHFIGKDILYFHTLF
WPAMLEFSGHRTPTNVFAHGFLTVDGAKMSKSRGTFITAQSYIDTGLNPEWLRYYFAAKLNATMEDIDLNLDDFQARVNS
DLVGKYVNIASRAAGFLIKRFDGRVQDSAMNHPLVAKLREAIPQIAAHYEAREYGRALRHTMELADEVNAYVDGAKPWDL
AKDPANAVALHETCSVSLESFRLLSLALKPVMPRVAEAVESFFGIAPLAWSDAAKPLSSEQPIKAYQHLMTRVDPKQIEA
LLAANRDSLQAEATGAAVATANAAKDAKNSAKANANAKQAVVNGADDAPISIDDFAKIDLRIAKIVACQAVEGSDKLLQL
TLDVGEEKTRNVFSGIKSAYQPEQLVGKLTVMVANLAPRKMKFGLSEGMVLAASATDEKAEPGLYILEPHSGAKPGMRVK

Sequences:

>Translated_720_residues
MSASDLTSVQAAAPQGDRQILVTSALPYANGQIHIGHLVEYIQTDIWVRTLRMHGHEVYYIGADDTHGTPIMLRAEKEGL
TPKQLIDRVWTEHKRDFDSFGVSFDNFYSTDSDENRVLSEKIYVALQDAGFIAEREIEQAYDPVKEMFLPDRFIKGECPK
CHAKDQYGDNCEVCGSTYLPTELLNPYSVVSGATPVRKTSKHYFFRLSDPRCETFLREWVSGLAQPEATNKMREWLGDAG
EAKLADWDISRDAPYFGFEIPGAPGKYFYVWLDAPVGYYASFKNLCDRNGVDFDAWVRPGSTTEQYHFIGKDILYFHTLF
WPAMLEFSGHRTPTNVFAHGFLTVDGAKMSKSRGTFITAQSYIDTGLNPEWLRYYFAAKLNATMEDIDLNLDDFQARVNS
DLVGKYVNIASRAAGFLIKRFDGRVQDSAMNHPLVAKLREAIPQIAAHYEAREYGRALRHTMELADEVNAYVDGAKPWDL
AKDPANAVALHETCSVSLESFRLLSLALKPVMPRVAEAVESFFGIAPLAWSDAAKPLSSEQPIKAYQHLMTRVDPKQIEA
LLAANRDSLQAEATGAAVATANAAKDAKNSAKANANAKQAVVNGADDAPISIDDFAKIDLRIAKIVACQAVEGSDKLLQL
TLDVGEEKTRNVFSGIKSAYQPEQLVGKLTVMVANLAPRKMKFGLSEGMVLAASATDEKAEPGLYILEPHSGAKPGMRVK
>Mature_719_residues
SASDLTSVQAAAPQGDRQILVTSALPYANGQIHIGHLVEYIQTDIWVRTLRMHGHEVYYIGADDTHGTPIMLRAEKEGLT
PKQLIDRVWTEHKRDFDSFGVSFDNFYSTDSDENRVLSEKIYVALQDAGFIAEREIEQAYDPVKEMFLPDRFIKGECPKC
HAKDQYGDNCEVCGSTYLPTELLNPYSVVSGATPVRKTSKHYFFRLSDPRCETFLREWVSGLAQPEATNKMREWLGDAGE
AKLADWDISRDAPYFGFEIPGAPGKYFYVWLDAPVGYYASFKNLCDRNGVDFDAWVRPGSTTEQYHFIGKDILYFHTLFW
PAMLEFSGHRTPTNVFAHGFLTVDGAKMSKSRGTFITAQSYIDTGLNPEWLRYYFAAKLNATMEDIDLNLDDFQARVNSD
LVGKYVNIASRAAGFLIKRFDGRVQDSAMNHPLVAKLREAIPQIAAHYEAREYGRALRHTMELADEVNAYVDGAKPWDLA
KDPANAVALHETCSVSLESFRLLSLALKPVMPRVAEAVESFFGIAPLAWSDAAKPLSSEQPIKAYQHLMTRVDPKQIEAL
LAANRDSLQAEATGAAVATANAAKDAKNSAKANANAKQAVVNGADDAPISIDDFAKIDLRIAKIVACQAVEGSDKLLQLT
LDVGEEKTRNVFSGIKSAYQPEQLVGKLTVMVANLAPRKMKFGLSEGMVLAASATDEKAEPGLYILEPHSGAKPGMRVK

Specific function: Is required not only for elongation of protein synthesis but also for the initiation of all mRNA translation through initiator tRNA(fMet) aminoacylation [H]

COG id: COG0143

COG function: function code J; Methionyl-tRNA synthetase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 tRNA-binding domain [H]

Homologues:

Organism=Homo sapiens, GI14043022, Length=606, Percent_Identity=27.2277227722772, Blast_Score=219, Evalue=5e-57,
Organism=Homo sapiens, GI24308436, Length=553, Percent_Identity=26.7631103074141, Blast_Score=160, Evalue=4e-39,
Organism=Homo sapiens, GI215490009, Length=122, Percent_Identity=33.6065573770492, Blast_Score=70, Evalue=5e-12,
Organism=Homo sapiens, GI45006986, Length=122, Percent_Identity=33.6065573770492, Blast_Score=70, Evalue=5e-12,
Organism=Homo sapiens, GI215490011, Length=122, Percent_Identity=33.6065573770492, Blast_Score=70, Evalue=6e-12,
Organism=Escherichia coli, GI1788432, Length=710, Percent_Identity=52.8169014084507, Blast_Score=740, Evalue=0.0,
Organism=Caenorhabditis elegans, GI17540794, Length=606, Percent_Identity=26.8976897689769, Blast_Score=227, Evalue=2e-59,
Organism=Caenorhabditis elegans, GI212645096, Length=536, Percent_Identity=24.8134328358209, Blast_Score=141, Evalue=1e-33,
Organism=Caenorhabditis elegans, GI71992781, Length=430, Percent_Identity=23.4883720930233, Blast_Score=103, Evalue=2e-22,
Organism=Saccharomyces cerevisiae, GI6321704, Length=564, Percent_Identity=28.3687943262411, Blast_Score=231, Evalue=4e-61,
Organism=Saccharomyces cerevisiae, GI6321610, Length=544, Percent_Identity=24.6323529411765, Blast_Score=133, Evalue=9e-32,
Organism=Drosophila melanogaster, GI24655311, Length=614, Percent_Identity=29.1530944625407, Blast_Score=230, Evalue=3e-60,
Organism=Drosophila melanogaster, GI21358483, Length=552, Percent_Identity=26.9927536231884, Blast_Score=135, Evalue=7e-32,

Paralogues:

None

Copy number: 871 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001412
- InterPro:   IPR015413
- InterPro:   IPR004495
- InterPro:   IPR014758
- InterPro:   IPR012340
- InterPro:   IPR016027
- InterPro:   IPR014729
- InterPro:   IPR002547
- InterPro:   IPR009080
- InterPro:   IPR013155 [H]

Pfam domain/function: PF08264 Anticodon_1; PF09334 tRNA-synt_1g; PF01588 tRNA_bind [H]

EC number: =6.1.1.10 [H]

Molecular weight: Translated: 79833; Mature: 79702

Theoretical pI: Translated: 5.74; Mature: 5.74

Prosite motif: PS50886 TRBD ; PS00178 AA_TRNA_LIGASE_I

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSASDLTSVQAAAPQGDRQILVTSALPYANGQIHIGHLVEYIQTDIWVRTLRMHGHEVYY
CCCCHHCHHHHCCCCCCCEEEEEECCCCCCCCEEHHHHHHHHHHHHHHHHHHHCCCEEEE
IGADDTHGTPIMLRAEKEGLTPKQLIDRVWTEHKRDFDSFGVSFDNFYSTDSDENRVLSE
EECCCCCCCEEEEEECCCCCCHHHHHHHHHHHHHCCHHHHCCCHHHCCCCCCCCHHHHHH
KIYVALQDAGFIAEREIEQAYDPVKEMFLPDRFIKGECPKCHAKDQYGDNCEVCGSTYLP
EEEEEEECCCCHHHHHHHHHHHHHHHHHCCCHHHCCCCCCCCCCCCCCCCCCCCCCCCCC
TELLNPYSVVSGATPVRKTSKHYFFRLSDPRCETFLREWVSGLAQPEATNKMREWLGDAG
HHHCCCHHHHCCCCCCHHCCCCEEEEECCCHHHHHHHHHHHHHCCCHHHHHHHHHHCCCC
EAKLADWDISRDAPYFGFEIPGAPGKYFYVWLDAPVGYYASFKNLCDRNGVDFDAWVRPG
CCEEECCCCCCCCCCCCEECCCCCCCEEEEEECCCCCHHHHHHHHHHCCCCCHHHEECCC
STTEQYHFIGKDILYFHTLFWPAMLEFSGHRTPTNVFAHGFLTVDGAKMSKSRGTFITAQ
CCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHEEEEEEEECCCCCCCCCCCEEEEH
SYIDTGLNPEWLRYYFAAKLNATMEDIDLNLDDFQARVNSDLVGKYVNIASRAAGFLIKR
HHHCCCCCHHHHHHHHHHHHCCCHHHHCCCHHHHHHHHCHHHHHHHHHHHHHHHHHHHHH
FDGRVQDSAMNHPLVAKLREAIPQIAAHYEAREYGRALRHTMELADEVNAYVDGAKPWDL
HCCCHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCC
AKDPANAVALHETCSVSLESFRLLSLALKPVMPRVAEAVESFFGIAPLAWSDAAKPLSSE
CCCCCCCEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCC
QPIKAYQHLMTRVDPKQIEALLAANRDSLQAEATGAAVATANAAKDAKNSAKANANAKQA
CHHHHHHHHHHCCCHHHHHHHHHCCCCCCCHHCCCCEEEECCHHHHHHHHHCCCCCHHHH
VVNGADDAPISIDDFAKIDLRIAKIVACQAVEGSDKLLQLTLDVGEEKTRNVFSGIKSAY
EECCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCEEEEEECCCCHHHHHHHHHHHHCC
QPEQLVGKLTVMVANLAPRKMKFGLSEGMVLAASATDEKAEPGLYILEPHSGAKPGMRVK
CHHHHHHHHHHHHHHCCCHHHHCCCCCCEEEEEECCCCCCCCCEEEECCCCCCCCCCCCC
>Mature Secondary Structure 
SASDLTSVQAAAPQGDRQILVTSALPYANGQIHIGHLVEYIQTDIWVRTLRMHGHEVYY
CCCHHCHHHHCCCCCCCEEEEEECCCCCCCCEEHHHHHHHHHHHHHHHHHHHCCCEEEE
IGADDTHGTPIMLRAEKEGLTPKQLIDRVWTEHKRDFDSFGVSFDNFYSTDSDENRVLSE
EECCCCCCCEEEEEECCCCCCHHHHHHHHHHHHHCCHHHHCCCHHHCCCCCCCCHHHHHH
KIYVALQDAGFIAEREIEQAYDPVKEMFLPDRFIKGECPKCHAKDQYGDNCEVCGSTYLP
EEEEEEECCCCHHHHHHHHHHHHHHHHHCCCHHHCCCCCCCCCCCCCCCCCCCCCCCCCC
TELLNPYSVVSGATPVRKTSKHYFFRLSDPRCETFLREWVSGLAQPEATNKMREWLGDAG
HHHCCCHHHHCCCCCCHHCCCCEEEEECCCHHHHHHHHHHHHHCCCHHHHHHHHHHCCCC
EAKLADWDISRDAPYFGFEIPGAPGKYFYVWLDAPVGYYASFKNLCDRNGVDFDAWVRPG
CCEEECCCCCCCCCCCCEECCCCCCCEEEEEECCCCCHHHHHHHHHHCCCCCHHHEECCC
STTEQYHFIGKDILYFHTLFWPAMLEFSGHRTPTNVFAHGFLTVDGAKMSKSRGTFITAQ
CCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHEEEEEEEECCCCCCCCCCCEEEEH
SYIDTGLNPEWLRYYFAAKLNATMEDIDLNLDDFQARVNSDLVGKYVNIASRAAGFLIKR
HHHCCCCCHHHHHHHHHHHHCCCHHHHCCCHHHHHHHHCHHHHHHHHHHHHHHHHHHHHH
FDGRVQDSAMNHPLVAKLREAIPQIAAHYEAREYGRALRHTMELADEVNAYVDGAKPWDL
HCCCHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCC
AKDPANAVALHETCSVSLESFRLLSLALKPVMPRVAEAVESFFGIAPLAWSDAAKPLSSE
CCCCCCCEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCC
QPIKAYQHLMTRVDPKQIEALLAANRDSLQAEATGAAVATANAAKDAKNSAKANANAKQA
CHHHHHHHHHHCCCHHHHHHHHHCCCCCCCHHCCCCEEEECCHHHHHHHHHCCCCCHHHH
VVNGADDAPISIDDFAKIDLRIAKIVACQAVEGSDKLLQLTLDVGEEKTRNVFSGIKSAY
EECCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCEEEEEECCCCHHHHHHHHHHHHCC
QPEQLVGKLTVMVANLAPRKMKFGLSEGMVLAASATDEKAEPGLYILEPHSGAKPGMRVK
CHHHHHHHHHHHHHHCCCHHHHCCCCCCEEEEEECCCCCCCCCEEEECCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA