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The map label for this gene is rfaQ [H]

Identifier: 78067122

GI number: 78067122

Start: 2751551

End: 2752765

Strand: Reverse

Name: rfaQ [H]

Synonym: Bcep18194_A5653

Alternate gene names: 78067122

Gene position: 2752765-2751551 (Counterclockwise)

Preceding gene: 78067123

Following gene: 78067118

Centisome position: 74.52

GC content: 71.03

Gene sequence:

>1215_bases
TTGCCAGCCTTGTTCAGCCAGCCAGACACACCCGCCGTGGCCCTGTTTTCTTCTGCCCGCCCGCCCAGAACCATCCTCGT
TGCCGCGCCGCGCCGCATTGGCGACGTGCTGCTGACGACGCCGCTCGTCCGCTCGCTGAAGGCCCGCTGGCCGGACGCGC
AGATCGACATGCTGGTGTTTCGCGGCACCGAAGGCGTGCTCGAGCACAATCCGGACGTACGGCGCGTGATCGTCGTCGCG
CAGCGCGCGGGAATCCGCGAGCGGCTGCGCGACGCACTGTCGATGTGGCGCCGTTACGATCTCGCGTGCGCGGCGCTGAG
CTCCGACCGGCCGCGCTTCTACAGCTGGTTCGCGGGCCGCAAGCGGGTGGGCCTCGTCGATCCGAACCGCGTGACCTGGC
TCACGCGGATGATGCTGAACGGGATCGCGATCAATCACCACGAATCCGCGCACACGGTCGTCAGCACGCTTGCACTCGCT
CCCGTGATCGGCATCGAGCCGGTGTCCGAGGTCGTCGCGCCCGGCATCGGTGACGATCCGGCGCGGCGCGCGCGCTTCGG
CGCGTGGCTTGCCGAATCGCCGGCGATCCGCGACGGCAAGCCGCTGGTCGTCCTGCATCCGTATCCGATGTTCCGTTACA
AGCAATGGCGGCTCGACGGCTGGGTCGAGATGATCGGCTGGCTGCGCGAGCACGGCTTCGCGGTCGCGCTGTCCGGCGGC
CCGGCCGACAGCGAGCGCGAGTATGCGGAGCAGGTTGCGGCCGAAGCGGGCGGCGACGTGCTGAACCTGGTCGGCCGCCT
GACGTTCGGCGAAAGCGCCGAACTCGTGCGGCGCGCGCGGCTTTTCATCGGGCCCGACACCGGCGCGACGCACGTCGCGG
CCGCGACCGGCACCGACACGATCGCGCTGTTCGGCCCGTCCGACCCGGTGCGCTGGGGCCCGTGGCCGCAGCACTGGCCG
GCGACCGAGAATCCGTGGCCGCTGCGCGGCTCGGGGCGGCACGGGAACGTGTGGCTGCTGCAGGGGGAGGGCGATTGCGT
GCCGTGCCGGCACGAAGGCTGCGAGCGCAAGGTCGACAGCCGCAGCGACTGCCTCGCGAATCTCGGTGCGCAGCGCGTGA
AAGCCGCGGCGGCCGAGATGCTCGGGCTGAATCCGCCGGGCCCGGCCGAAGCCGTCGTCGATACGTCGCGACTGCATCGC
AGCGGATCCGACTGA

Upstream 100 bases:

>100_bases
GCGGTGCGCCGGCCTCGCCGCACCCCGCGAGCGGGGCCGCATCAGGCCAGCCGCACGGCCGATTCGCCACGCCGCGCCGG
CTGCTGCAAAATCCTGTACA

Downstream 100 bases:

>100_bases
CGCGCGGCCAACGCGCGTTGCCTGCCGGTTGCAGGCGGCTTGTCTGGCGACGAGCCAGCCGCGCAGCCCGTTTCCTTCGA
GGGTAGAGAACTCCGCGGTG

Product: glycosyl transferase family protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 404; Mature: 403

Protein sequence:

>404_residues
MPALFSQPDTPAVALFSSARPPRTILVAAPRRIGDVLLTTPLVRSLKARWPDAQIDMLVFRGTEGVLEHNPDVRRVIVVA
QRAGIRERLRDALSMWRRYDLACAALSSDRPRFYSWFAGRKRVGLVDPNRVTWLTRMMLNGIAINHHESAHTVVSTLALA
PVIGIEPVSEVVAPGIGDDPARRARFGAWLAESPAIRDGKPLVVLHPYPMFRYKQWRLDGWVEMIGWLREHGFAVALSGG
PADSEREYAEQVAAEAGGDVLNLVGRLTFGESAELVRRARLFIGPDTGATHVAAATGTDTIALFGPSDPVRWGPWPQHWP
ATENPWPLRGSGRHGNVWLLQGEGDCVPCRHEGCERKVDSRSDCLANLGAQRVKAAAAEMLGLNPPGPAEAVVDTSRLHR
SGSD

Sequences:

>Translated_404_residues
MPALFSQPDTPAVALFSSARPPRTILVAAPRRIGDVLLTTPLVRSLKARWPDAQIDMLVFRGTEGVLEHNPDVRRVIVVA
QRAGIRERLRDALSMWRRYDLACAALSSDRPRFYSWFAGRKRVGLVDPNRVTWLTRMMLNGIAINHHESAHTVVSTLALA
PVIGIEPVSEVVAPGIGDDPARRARFGAWLAESPAIRDGKPLVVLHPYPMFRYKQWRLDGWVEMIGWLREHGFAVALSGG
PADSEREYAEQVAAEAGGDVLNLVGRLTFGESAELVRRARLFIGPDTGATHVAAATGTDTIALFGPSDPVRWGPWPQHWP
ATENPWPLRGSGRHGNVWLLQGEGDCVPCRHEGCERKVDSRSDCLANLGAQRVKAAAAEMLGLNPPGPAEAVVDTSRLHR
SGSD
>Mature_403_residues
PALFSQPDTPAVALFSSARPPRTILVAAPRRIGDVLLTTPLVRSLKARWPDAQIDMLVFRGTEGVLEHNPDVRRVIVVAQ
RAGIRERLRDALSMWRRYDLACAALSSDRPRFYSWFAGRKRVGLVDPNRVTWLTRMMLNGIAINHHESAHTVVSTLALAP
VIGIEPVSEVVAPGIGDDPARRARFGAWLAESPAIRDGKPLVVLHPYPMFRYKQWRLDGWVEMIGWLREHGFAVALSGGP
ADSEREYAEQVAAEAGGDVLNLVGRLTFGESAELVRRARLFIGPDTGATHVAAATGTDTIALFGPSDPVRWGPWPQHWPA
TENPWPLRGSGRHGNVWLLQGEGDCVPCRHEGCERKVDSRSDCLANLGAQRVKAAAAEMLGLNPPGPAEAVVDTSRLHRS
GSD

Specific function: Catalyzes heptose transfer to the lipopolysaccharide core. It transfers a heptose, called heptose(III), to the heptose(II) of the inner core [H]

COG id: COG0859

COG function: function code M; ADP-heptose:LPS heptosyltransferase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the glycosyltransferase 9 family [H]

Homologues:

Organism=Escherichia coli, GI1790062, Length=367, Percent_Identity=26.4305177111717, Blast_Score=115, Evalue=4e-27,
Organism=Escherichia coli, GI1790050, Length=298, Percent_Identity=26.1744966442953, Blast_Score=77, Evalue=2e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002201
- InterPro:   IPR011916 [H]

Pfam domain/function: PF01075 Glyco_transf_9 [H]

EC number: 2.-.-.- [C]

Molecular weight: Translated: 44249; Mature: 44118

Theoretical pI: Translated: 8.75; Mature: 8.75

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPALFSQPDTPAVALFSSARPPRTILVAAPRRIGDVLLTTPLVRSLKARWPDAQIDMLVF
CCCCCCCCCCCCEEEECCCCCCCEEEEECCHHHHHHHHHHHHHHHHHHCCCCCCEEEEEE
RGTEGVLEHNPDVRRVIVVAQRAGIRERLRDALSMWRRYDLACAALSSDRPRFYSWFAGR
ECCCCHHHCCCCCEEEEEEHHHCCHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHCCC
KRVGLVDPNRVTWLTRMMLNGIAINHHESAHTVVSTLALAPVIGIEPVSEVVAPGIGDDP
CEECCCCCCHHHHHHHHHHCCEEEECCCHHHHHHHHHHHHHHCCCCCHHHHHCCCCCCCH
ARRARFGAWLAESPAIRDGKPLVVLHPYPMFRYKQWRLDGWVEMIGWLREHGFAVALSGG
HHHHHHHHHHHCCCCCCCCCCEEEECCCCCCHHHHHHHHHHHHHHHHHHHCCEEEEECCC
PADSEREYAEQVAAEAGGDVLNLVGRLTFGESAELVRRARLFIGPDTGATHVAAATGTDT
CCCHHHHHHHHHHHHCCCHHHHHHHHCCCCCHHHHHHHHHHEECCCCCCCEEEEECCCCE
IALFGPSDPVRWGPWPQHWPATENPWPLRGSGRHGNVWLLQGEGDCVPCRHEGCERKVDS
EEEECCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCEEEEECCCCEECCCCCCHHHCCCC
RSDCLANLGAQRVKAAAAEMLGLNPPGPAEAVVDTSRLHRSGSD
HHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHCCCCC
>Mature Secondary Structure 
PALFSQPDTPAVALFSSARPPRTILVAAPRRIGDVLLTTPLVRSLKARWPDAQIDMLVF
CCCCCCCCCCCEEEECCCCCCCEEEEECCHHHHHHHHHHHHHHHHHHCCCCCCEEEEEE
RGTEGVLEHNPDVRRVIVVAQRAGIRERLRDALSMWRRYDLACAALSSDRPRFYSWFAGR
ECCCCHHHCCCCCEEEEEEHHHCCHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHCCC
KRVGLVDPNRVTWLTRMMLNGIAINHHESAHTVVSTLALAPVIGIEPVSEVVAPGIGDDP
CEECCCCCCHHHHHHHHHHCCEEEECCCHHHHHHHHHHHHHHCCCCCHHHHHCCCCCCCH
ARRARFGAWLAESPAIRDGKPLVVLHPYPMFRYKQWRLDGWVEMIGWLREHGFAVALSGG
HHHHHHHHHHHCCCCCCCCCCEEEECCCCCCHHHHHHHHHHHHHHHHHHHCCEEEEECCC
PADSEREYAEQVAAEAGGDVLNLVGRLTFGESAELVRRARLFIGPDTGATHVAAATGTDT
CCCHHHHHHHHHHHHCCCHHHHHHHHCCCCCHHHHHHHHHHEECCCCCCCEEEEECCCCE
IALFGPSDPVRWGPWPQHWPATENPWPLRGSGRHGNVWLLQGEGDCVPCRHEGCERKVDS
EEEECCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCEEEEECCCCEECCCCCCHHHCCCC
RSDCLANLGAQRVKAAAAEMLGLNPPGPAEAVVDTSRLHRSGSD
HHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: Glycosyltransferases; Transferring other glycosyl groups [C]

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 1732225; 1447141; 8041620; 9278503 [H]