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The map label for this gene is shiA [H]

Identifier: 78066936

GI number: 78066936

Start: 2549632

End: 2551047

Strand: Reverse

Name: shiA [H]

Synonym: Bcep18194_A5467

Alternate gene names: 78066936

Gene position: 2551047-2549632 (Counterclockwise)

Preceding gene: 78066937

Following gene: 78066933

Centisome position: 69.06

GC content: 68.36

Gene sequence:

>1416_bases
ATGAGCCGTCAGCAATCCCCGACTACCGCACCGCAACCGCGGCCCGGCCGCGCCGCCTTCGCGGCGTTCGTCGGCACCAC
GATCGAGTGGTACGACTTCTACATCTACGGCACGGCCGCGGCGCTCGTGTTCGGCAAGGTGTTCTTTGCGAGCACAATGG
ATCCCGGCGTTGCGACGCTGCTGGCGTTCGTTACGTTCTGGGCCGGCTTTGCCGCGCGACCGCTCGGCGGCATCGTGTTC
GGGCATCTCGGCGATCGTGTCGGCCGCAAGACCGCGCTCGTGATCACGCTGGTGATGATGGGGCTGGCCACGACCGGCAT
CGGCCTGTTGCCCGGTTATGCGCAGATCGGCGTCTGGGCGCCGGCCGGCCTCGTCGTGCTGCGCGTGCTGCAGGGCATCG
CGGTCGGCGGCGAGTGGGGCGGCGCGGTGCTGATCGCGAGCGAGAACGCGCCGAAGCATCGCAGCATCCTGTATGCGGCG
TTCGCGCAGCAGGGCTCGCCCACCGGCAACCTGCTCGCGACTGCCGCGTTCTTCGCGCTGAGCGCGCTGCCGACGCCGTC
GTTCCTGATGTGGGGCTGGCGGATTCCGTTCCTGCTGTCGGCCGTGCTCGTGATCATCGGCATGGTGATCCGGCTGAAGC
TCGAGGAGTCGGCCGACATGCAGCGCGTGCTCGCGCGCAAGCGCACCGTGAAGCTGCCGCTGCGCGACGTCGTGCGCGAT
CACTGGGTCGTCGTGCTGCTCGCGGCCGGCACGCTGCCGGTGATCAACGTCACGTACTTCCGCAGCACGTTCGCGCTGTC
GTGGGCGACCAAGGAGCTCGGCTACGCGCAGGGTACGTTCCTCGGCATCCTGTCGATCTCGCTCGTCGTGCAGTTCCTGA
TGCAGCCGGTCGGCGCGTGGTTCGTGTCGAAGGTCGACATGCGCCGCGCGATGTGCTGGATCCTGATTCCGGAAATCGTG
CTGATGCCCGTGATGTTTCACGCGCTCGCGACACGGTCGTACTGGGTGGCCGTCGCGGGCATGTGCATCTCGACGATTCC
ATCGGCGATGTTCTATGGCGCGGTCGGCGGCGTGCTCGCACGCGTGTTCCCGGCGAACATCCGCTATACGGGCCTGTCGC
TCGCGTATCAGCTGAGCGCGCTCGTCGTCGGCGGCGGCACGCCCGTGCTCGCGCAGGCGATCCTCAATTCGACCGGCAGC
ATCGTCGGTGTCGCGGTTGCGTCGGGGCTTTACGCGTTCGTGTCGCTCGTCTGCATGCTGGCGCTGCTGAATCGCACCGG
ATATCGCGCGGACGAACTGTCGACGGCCGAACGCAGCGACGCGGCCGAATGGGGCACCGAAGGCGGCGGCGTGGAGCCCG
CGGTCGACGGGAACACGCAGCCAGGCGAGGGCGGCGCGCTGAAGCCGGCCGGTTGA

Upstream 100 bases:

>100_bases
CCGCCGACCGGCACGACGCCGCCGGCCGGCGGCGCGATGCCGGCCACCCGGGAAAGGCGCGCGACAGTCGCGCCCCCGAC
ACGCAAACATGGAGACATCG

Downstream 100 bases:

>100_bases
GCGGAGCGGCATACCGCCTCGCGCGTTCCCAAAAGAAAACGGCACCGTGCGAAACACGGTGCCGTCTTTCCCGGAGCGCG
TGGCCGGCAACTTGCGCCGG

Product: major facilitator transporter

Products: Proton [Cytoplasm]; shikimate [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 471; Mature: 470

Protein sequence:

>471_residues
MSRQQSPTTAPQPRPGRAAFAAFVGTTIEWYDFYIYGTAAALVFGKVFFASTMDPGVATLLAFVTFWAGFAARPLGGIVF
GHLGDRVGRKTALVITLVMMGLATTGIGLLPGYAQIGVWAPAGLVVLRVLQGIAVGGEWGGAVLIASENAPKHRSILYAA
FAQQGSPTGNLLATAAFFALSALPTPSFLMWGWRIPFLLSAVLVIIGMVIRLKLEESADMQRVLARKRTVKLPLRDVVRD
HWVVVLLAAGTLPVINVTYFRSTFALSWATKELGYAQGTFLGILSISLVVQFLMQPVGAWFVSKVDMRRAMCWILIPEIV
LMPVMFHALATRSYWVAVAGMCISTIPSAMFYGAVGGVLARVFPANIRYTGLSLAYQLSALVVGGGTPVLAQAILNSTGS
IVGVAVASGLYAFVSLVCMLALLNRTGYRADELSTAERSDAAEWGTEGGGVEPAVDGNTQPGEGGALKPAG

Sequences:

>Translated_471_residues
MSRQQSPTTAPQPRPGRAAFAAFVGTTIEWYDFYIYGTAAALVFGKVFFASTMDPGVATLLAFVTFWAGFAARPLGGIVF
GHLGDRVGRKTALVITLVMMGLATTGIGLLPGYAQIGVWAPAGLVVLRVLQGIAVGGEWGGAVLIASENAPKHRSILYAA
FAQQGSPTGNLLATAAFFALSALPTPSFLMWGWRIPFLLSAVLVIIGMVIRLKLEESADMQRVLARKRTVKLPLRDVVRD
HWVVVLLAAGTLPVINVTYFRSTFALSWATKELGYAQGTFLGILSISLVVQFLMQPVGAWFVSKVDMRRAMCWILIPEIV
LMPVMFHALATRSYWVAVAGMCISTIPSAMFYGAVGGVLARVFPANIRYTGLSLAYQLSALVVGGGTPVLAQAILNSTGS
IVGVAVASGLYAFVSLVCMLALLNRTGYRADELSTAERSDAAEWGTEGGGVEPAVDGNTQPGEGGALKPAG
>Mature_470_residues
SRQQSPTTAPQPRPGRAAFAAFVGTTIEWYDFYIYGTAAALVFGKVFFASTMDPGVATLLAFVTFWAGFAARPLGGIVFG
HLGDRVGRKTALVITLVMMGLATTGIGLLPGYAQIGVWAPAGLVVLRVLQGIAVGGEWGGAVLIASENAPKHRSILYAAF
AQQGSPTGNLLATAAFFALSALPTPSFLMWGWRIPFLLSAVLVIIGMVIRLKLEESADMQRVLARKRTVKLPLRDVVRDH
WVVVLLAAGTLPVINVTYFRSTFALSWATKELGYAQGTFLGILSISLVVQFLMQPVGAWFVSKVDMRRAMCWILIPEIVL
MPVMFHALATRSYWVAVAGMCISTIPSAMFYGAVGGVLARVFPANIRYTGLSLAYQLSALVVGGGTPVLAQAILNSTGSI
VGVAVASGLYAFVSLVCMLALLNRTGYRADELSTAERSDAAEWGTEGGGVEPAVDGNTQPGEGGALKPAG

Specific function: Unknown

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the major facilitator superfamily. Sugar transporter (TC 2.A.1.1) family [H]

Homologues:

Organism=Escherichia coli, GI1788292, Length=440, Percent_Identity=37.7272727272727, Blast_Score=245, Evalue=4e-66,
Organism=Escherichia coli, GI1789941, Length=399, Percent_Identity=38.8471177944862, Blast_Score=213, Evalue=2e-56,
Organism=Escherichia coli, GI1790550, Length=438, Percent_Identity=31.0502283105023, Blast_Score=190, Evalue=2e-49,
Organism=Escherichia coli, GI1788942, Length=422, Percent_Identity=31.042654028436, Blast_Score=157, Evalue=1e-39,
Organism=Escherichia coli, GI87082231, Length=285, Percent_Identity=25.9649122807018, Blast_Score=77, Evalue=2e-15,
Organism=Escherichia coli, GI87082404, Length=441, Percent_Identity=24.9433106575964, Blast_Score=62, Evalue=7e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004736
- InterPro:   IPR020846
- InterPro:   IPR016196
- InterPro:   IPR005828
- InterPro:   IPR005829 [H]

Pfam domain/function: PF00083 Sugar_tr [H]

EC number: NA

Molecular weight: Translated: 49884; Mature: 49753

Theoretical pI: Translated: 9.91; Mature: 9.91

Prosite motif: PS00024 HEMOPEXIN ; PS50850 MFS

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
3.2 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSRQQSPTTAPQPRPGRAAFAAFVGTTIEWYDFYIYGTAAALVFGKVFFASTMDPGVATL
CCCCCCCCCCCCCCCCHHHHHHHHCCEEEHHEEEEHHHHHHHHHHHHHHHHCCCCHHHHH
LAFVTFWAGFAARPLGGIVFGHLGDRVGRKTALVITLVMMGLATTGIGLLPGYAQIGVWA
HHHHHHHHHHHCCCHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHCCCHHCCCHHHHCCCC
PAGLVVLRVLQGIAVGGEWGGAVLIASENAPKHRSILYAAFAQQGSPTGNLLATAAFFAL
HHHHHHHHHHHHHCCCCCCCCEEEEECCCCCCHHHHHHHHHHHCCCCCCHHHHHHHHHHH
SALPTPSFLMWGWRIPFLLSAVLVIIGMVIRLKLEESADMQRVLARKRTVKLPLRDVVRD
HCCCCCCHHHHHCCHHHHHHHHHHHHHHHHHEECCCCHHHHHHHHHHHCCCCCHHHHHHH
HWVVVLLAAGTLPVINVTYFRSTFALSWATKELGYAQGTFLGILSISLVVQFLMQPVGAW
HHEEEEEECCCCCEEEEHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHH
FVSKVDMRRAMCWILIPEIVLMPVMFHALATRSYWVAVAGMCISTIPSAMFYGAVGGVLA
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
RVFPANIRYTGLSLAYQLSALVVGGGTPVLAQAILNSTGSIVGVAVASGLYAFVSLVCML
HHHCCCCEEEHHHHHHEEEEEEECCCCHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHH
ALLNRTGYRADELSTAERSDAAEWGTEGGGVEPAVDGNTQPGEGGALKPAG
HHHHCCCCCHHHCCCHHHCCHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCC
>Mature Secondary Structure 
SRQQSPTTAPQPRPGRAAFAAFVGTTIEWYDFYIYGTAAALVFGKVFFASTMDPGVATL
CCCCCCCCCCCCCCCHHHHHHHHCCEEEHHEEEEHHHHHHHHHHHHHHHHCCCCHHHHH
LAFVTFWAGFAARPLGGIVFGHLGDRVGRKTALVITLVMMGLATTGIGLLPGYAQIGVWA
HHHHHHHHHHHCCCHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHCCCHHCCCHHHHCCCC
PAGLVVLRVLQGIAVGGEWGGAVLIASENAPKHRSILYAAFAQQGSPTGNLLATAAFFAL
HHHHHHHHHHHHHCCCCCCCCEEEEECCCCCCHHHHHHHHHHHCCCCCCHHHHHHHHHHH
SALPTPSFLMWGWRIPFLLSAVLVIIGMVIRLKLEESADMQRVLARKRTVKLPLRDVVRD
HCCCCCCHHHHHCCHHHHHHHHHHHHHHHHHEECCCCHHHHHHHHHHHCCCCCHHHHHHH
HWVVVLLAAGTLPVINVTYFRSTFALSWATKELGYAQGTFLGILSISLVVQFLMQPVGAW
HHEEEEEECCCCCEEEEHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHH
FVSKVDMRRAMCWILIPEIVLMPVMFHALATRSYWVAVAGMCISTIPSAMFYGAVGGVLA
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
RVFPANIRYTGLSLAYQLSALVVGGGTPVLAQAILNSTGSIVGVAVASGLYAFVSLVCML
HHHCCCCEEEHHHHHHEEEEEEECCCCHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHH
ALLNRTGYRADELSTAERSDAAEWGTEGGGVEPAVDGNTQPGEGGALKPAG
HHHHCCCCCHHHCCCHHHCCHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Proton [Periplasm]; shikimate [Periplasm] [C]

Specific reaction: Proton [Periplasm] + shikimate [Periplasm] = Proton [Cytoplasm] + shikimate [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 9524262; 9097040; 9278503 [H]