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The map label for this gene is ntrB [H]

Identifier: 78066926

GI number: 78066926

Start: 2535961

End: 2537103

Strand: Reverse

Name: ntrB [H]

Synonym: Bcep18194_A5457

Alternate gene names: 78066926

Gene position: 2537103-2535961 (Counterclockwise)

Preceding gene: 78066927

Following gene: 78066925

Centisome position: 68.68

GC content: 65.88

Gene sequence:

>1143_bases
ATGGTTCTGAAGAATCTGATCAAGGCGAAAACGGGGCAGCCCGAGCGACTGACGGACGACGAGCGGCTCTCGCGCTCGGG
CCTGCTGGCGGGGCTGGAAGCGTTGCCGACGGTCGTGATCGTGCTCGACCGCAAGACGCTGCGGATCGCGTTCGCGAACC
CGTCAGCCGAGGCGATGCTCGACATTTCGCGCCGGCAGCTCGCGCAGCGGCCGTGGGGCGAGATTTTTCCGAATGCGAAC
GAGCTGGCATCGACGATCACCGCGATCGGCGAAGAACGCTTTCATGCGACGCACCTCGATACCGTGCTCGACCGGCCCGG
CCGCGAACCGCTGCATGTGCACGCGATCGTCGGCTTTCTCGAGACCTCGCCCGATTTCGTGCTCGTCGAGCTGTTCGAGA
ACGAGCGGCAGTCGCGCACCGATCGCGAGGAGCGCATCCACGACCTGACCGCGGTCAACAAGCAGTTGATCCGCAACCTC
GCGCACGAGATCAAGAACCCGCTCGGCGGCATTCGCGGCGCAGCCCAGCTGCTCGAATTCGAGCTCGGCGAGCGCGAGCG
CGGCGAGTTGCGCGAATACACGCAGGTGATCATCAAGGAGTCCGACCGCCTGCAGACGCTCGTCGACCGGTTGCTGGAGC
CGCACCGGCATCCGCACATCGTCGGCGACGTGAACATTCATGAAGTATGCGAACGCGTGCGTGCGGTGATGCTCGCGGAA
TTCCCGCGCGGGCTCTCGATCGAGCGCGACTACGACGTGAGCGTGCCGGACCTGCGCGGCGACAAGGAGCAACTGATCCA
GGCGCTGCTCAACATCGTGCGCAACGCCGCGCAGGCGCTGCGCGAACGGATCGCGCAGGGCGACGCGAAGATCGAGCTGC
GCACGCGCATCGCGCGCAAGGTGACGATCGCGAAGCGCCTGTACCGGCTGGCACTGGACTTGCACGTGATCGACAACGGG
CCCGGCATTCCGGAGGAAATCCGCGACCGGATCTTCTACCCGCTCGTGTCCGGGCGCGACGACGGCAGCGGCCTCGGCCT
GACGCTCGCGCAGACGTTCGTGCAGCAGCACGACGGGATGATCGAGGTCGAAAGCCGGCCCGGACGTACCGAATTTCAGA
TCCTGCTGCCGCTCGACCATTGA

Upstream 100 bases:

>100_bases
GGCGGGAACGGCGGAGTCTGAAAGGGGCGGCGTCCAGCCGTCCCTTTTTTGTTCCGGTGATTTTCCTTTTCTGGCCCGTA
AAGGCGCGACAAGCACGCAG

Downstream 100 bases:

>100_bases
GCGGCGGCATGGCGATTCACCAGATTCACCAATATCTGACCGACCTATGAAGCCGATCTGGATAGTAGACGACGACCAAT
CGATCCGCTGGGTGCTCGAA

Product: signal transduction histidine kinase, nitrogen specific, NtrB

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 380; Mature: 380

Protein sequence:

>380_residues
MVLKNLIKAKTGQPERLTDDERLSRSGLLAGLEALPTVVIVLDRKTLRIAFANPSAEAMLDISRRQLAQRPWGEIFPNAN
ELASTITAIGEERFHATHLDTVLDRPGREPLHVHAIVGFLETSPDFVLVELFENERQSRTDREERIHDLTAVNKQLIRNL
AHEIKNPLGGIRGAAQLLEFELGERERGELREYTQVIIKESDRLQTLVDRLLEPHRHPHIVGDVNIHEVCERVRAVMLAE
FPRGLSIERDYDVSVPDLRGDKEQLIQALLNIVRNAAQALRERIAQGDAKIELRTRIARKVTIAKRLYRLALDLHVIDNG
PGIPEEIRDRIFYPLVSGRDDGSGLGLTLAQTFVQQHDGMIEVESRPGRTEFQILLPLDH

Sequences:

>Translated_380_residues
MVLKNLIKAKTGQPERLTDDERLSRSGLLAGLEALPTVVIVLDRKTLRIAFANPSAEAMLDISRRQLAQRPWGEIFPNAN
ELASTITAIGEERFHATHLDTVLDRPGREPLHVHAIVGFLETSPDFVLVELFENERQSRTDREERIHDLTAVNKQLIRNL
AHEIKNPLGGIRGAAQLLEFELGERERGELREYTQVIIKESDRLQTLVDRLLEPHRHPHIVGDVNIHEVCERVRAVMLAE
FPRGLSIERDYDVSVPDLRGDKEQLIQALLNIVRNAAQALRERIAQGDAKIELRTRIARKVTIAKRLYRLALDLHVIDNG
PGIPEEIRDRIFYPLVSGRDDGSGLGLTLAQTFVQQHDGMIEVESRPGRTEFQILLPLDH
>Mature_380_residues
MVLKNLIKAKTGQPERLTDDERLSRSGLLAGLEALPTVVIVLDRKTLRIAFANPSAEAMLDISRRQLAQRPWGEIFPNAN
ELASTITAIGEERFHATHLDTVLDRPGREPLHVHAIVGFLETSPDFVLVELFENERQSRTDREERIHDLTAVNKQLIRNL
AHEIKNPLGGIRGAAQLLEFELGERERGELREYTQVIIKESDRLQTLVDRLLEPHRHPHIVGDVNIHEVCERVRAVMLAE
FPRGLSIERDYDVSVPDLRGDKEQLIQALLNIVRNAAQALRERIAQGDAKIELRTRIARKVTIAKRLYRLALDLHVIDNG
PGIPEEIRDRIFYPLVSGRDDGSGLGLTLAQTFVQQHDGMIEVESRPGRTEFQILLPLDH

Specific function: NtrB acts as a signal transducer which responds to the nitrogen level of cell and modulates the activity of ntrC. In nitrogen limitation ntrB activates ntrC by phosphorylating it, while in nitrogen excess ntrC is dephosphorylated and consequently inactiva

COG id: COG3852

COG function: function code T; Signal transduction histidine kinase, nitrogen specific

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 PAS (PER-ARNT-SIM) domain [H]

Homologues:

Organism=Escherichia coli, GI1790300, Length=351, Percent_Identity=41.8803418803419, Blast_Score=225, Evalue=4e-60,
Organism=Escherichia coli, GI1788549, Length=357, Percent_Identity=27.7310924369748, Blast_Score=105, Evalue=4e-24,
Organism=Escherichia coli, GI1790436, Length=265, Percent_Identity=27.9245283018868, Blast_Score=97, Evalue=2e-21,
Organism=Escherichia coli, GI1790346, Length=243, Percent_Identity=27.5720164609054, Blast_Score=66, Evalue=3e-12,
Organism=Escherichia coli, GI87082128, Length=244, Percent_Identity=27.0491803278689, Blast_Score=62, Evalue=8e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003594
- InterPro:   IPR000014
- InterPro:   IPR013767
- InterPro:   IPR004358
- InterPro:   IPR003661
- InterPro:   IPR005467
- InterPro:   IPR009082 [H]

Pfam domain/function: PF02518 HATPase_c; PF00512 HisKA; PF00989 PAS [H]

EC number: =2.7.13.3 [H]

Molecular weight: Translated: 43079; Mature: 43079

Theoretical pI: Translated: 6.34; Mature: 6.34

Prosite motif: PS50109 HIS_KIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
1.1 %Met     (Translated Protein)
1.3 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
1.1 %Met     (Mature Protein)
1.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MVLKNLIKAKTGQPERLTDDERLSRSGLLAGLEALPTVVIVLDRKTLRIAFANPSAEAML
CCHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHCEEEEEECCCEEEEEECCCCHHHHH
DISRRQLAQRPWGEIFPNANELASTITAIGEERFHATHLDTVLDRPGREPLHVHAIVGFL
HHHHHHHHHCCCHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEHHHHHHHH
ETSPDFVLVELFENERQSRTDREERIHDLTAVNKQLIRNLAHEIKNPLGGIRGAAQLLEF
HCCCCEEEHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
ELGERERGELREYTQVIIKESDRLQTLVDRLLEPHRHPHIVGDVNIHEVCERVRAVMLAE
HCCCCCHHHHHHHHHHHHCCCHHHHHHHHHHHCCCCCCCEECCCCHHHHHHHHHHHHHHH
FPRGLSIERDYDVSVPDLRGDKEQLIQALLNIVRNAAQALRERIAQGDAKIELRTRIARK
CCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEHHHHHHHHH
VTIAKRLYRLALDLHVIDNGPGIPEEIRDRIFYPLVSGRDDGSGLGLTLAQTFVQQHDGM
HHHHHHHHHHHHHEEEECCCCCCCHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHCCCE
IEVESRPGRTEFQILLPLDH
EEECCCCCCCEEEEEEECCC
>Mature Secondary Structure
MVLKNLIKAKTGQPERLTDDERLSRSGLLAGLEALPTVVIVLDRKTLRIAFANPSAEAML
CCHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHCEEEEEECCCEEEEEECCCCHHHHH
DISRRQLAQRPWGEIFPNANELASTITAIGEERFHATHLDTVLDRPGREPLHVHAIVGFL
HHHHHHHHHCCCHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEHHHHHHHH
ETSPDFVLVELFENERQSRTDREERIHDLTAVNKQLIRNLAHEIKNPLGGIRGAAQLLEF
HCCCCEEEHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
ELGERERGELREYTQVIIKESDRLQTLVDRLLEPHRHPHIVGDVNIHEVCERVRAVMLAE
HCCCCCHHHHHHHHHHHHCCCHHHHHHHHHHHCCCCCCCEECCCCHHHHHHHHHHHHHHH
FPRGLSIERDYDVSVPDLRGDKEQLIQALLNIVRNAAQALRERIAQGDAKIELRTRIARK
CCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEHHHHHHHHH
VTIAKRLYRLALDLHVIDNGPGIPEEIRDRIFYPLVSGRDDGSGLGLTLAQTFVQQHDGM
HHHHHHHHHHHHHEEEECCCCCCCHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHCCCE
IEVESRPGRTEFQILLPLDH
EEECCCCCCCEEEEEEECCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 2574025 [H]