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The map label for this gene is glnG [H]

Identifier: 78066925

GI number: 78066925

Start: 2534394

End: 2535914

Strand: Reverse

Name: glnG [H]

Synonym: Bcep18194_A5456

Alternate gene names: 78066925

Gene position: 2535914-2534394 (Counterclockwise)

Preceding gene: 78066926

Following gene: 78066924

Centisome position: 68.65

GC content: 69.49

Gene sequence:

>1521_bases
ATGAAGCCGATCTGGATAGTAGACGACGACCAATCGATCCGCTGGGTGCTCGAAAAGGCGCTCGCCCGGGACAGCTTCGC
GACGAAGAGTTTCGCGAACGTGCGGGACGCGCTGGCCGCGCTCGACCACGAGACCCCGCAGGTGCTGGTATCCGACATCC
GGATGCCGGGCGGCTCGGGCCTCGAGTTGCTGCAGGCGATGCACGAGCGCCTGCCGGGCCTGCCCGTCATCATCATGACG
GCGTTCTCCGATCTCGACAGCGCCGTTGCGGCGTTCCAGGGCGGCGCGTTCGAATATCTCGCGAAGCCGTTCGACGTCGA
CAAGGCGGTCGAGCTGATCCGCCGTGCGGTCGAGGAAAGCCTGCGCGGCGGCGCGCCGCAGGACGAGCGCGTGGCCGAGG
CGCCCGAGATGCTCGGCCAGGCCCCCGCGATGCAGGACATGTTTCGCGCGATCGGTCGCCTGTCGCACTCGGCCGCGACC
GTGCTGATCACCGGTGAATCGGGCACCGGCAAGGAACTCGTCGCGCGTGCGCTGCACCGTCACAGCCCGCGTGCAAACGG
GCCGTTCATCGCGCTGAACACCGCGGCGATTCCGAAGGACCTGCTCGAATCCGAGCTGTTCGGCCATGAGCGCGGCGCGT
TCACCGGCGCGCAGACGACGCGGCAGGGCCGCTTCGAGCAGGCCGAGAACGGCACGCTGTTCCTCGACGAAATCGGCGAC
ATGCCGTTCGACCTGCAGACGCGCCTGTTGCGCGTGCTGTCGGACGGACAGTTCTATCGGGTCGGCGGCCACAACCCGTT
GCGCGCGAACGTGCGCGTGATCGCCGCTACCCACCAGAATCTCGAATCGCGCGTGCGGCAGGGGCTGTTCCGCGAGGACC
TTTACCACCGGCTCAACGTGATCCGGCTGCGCCTGCCGCCGCTGCGCGAGCGCAGCGAGGACATCGCGCTGCTCACGCGC
CACTTCCTGCAGAAGAGCGCGCGCGATCTCGGCGTCGAGCCGAAGCGCGTGTCCGACGAAGCGCTGGTGTACCTCACGTC
GCTGGCGTTTCCCGGCAACGTGCGCCAGCTCGAGAACCTCGCGAACTGGCTGACCGTGATGGCGCCCGCGCAGACGGTCG
AGATCAAGGACCTGCCGCCCGACCTCGTGCCGGCCGGTGCGCCGGTGGTTGCGACGAGCGACGGTGTGGACGCGCACGGC
GGCAGTGGCGAGGCGGCAACGCTCGCGCAACCGGCGATTGGCGGCGCTCCACTGGCGTCCGCACCGGTCGCGGCGGCGCC
GAACGGCAGCGCGCCGGCCGGCTATCCGCTGTGGGAGCACGGCCTGCGCACCGAGGTTGCACGGCTGTTGCGCGAGAACT
CGGCCGACGTGATGGACGAACTCGCACGCCGCTTCGAGGCGGCCGTGATCCGCGAGGCGCTCGACTTCACGCGCGGCCGC
AAGGTCGAGGCCGCGGAGCGGCTCGGCATCGGTCGCAACACGATCACGCGCAAGATCCAGGAACTCCATCTGGAGCCCTG
A

Upstream 100 bases:

>100_bases
GAAAGCCGGCCCGGACGTACCGAATTTCAGATCCTGCTGCCGCTCGACCATTGAGCGGCGGCATGGCGATTCACCAGATT
CACCAATATCTGACCGACCT

Downstream 100 bases:

>100_bases
GCACGATTTGCGGCATGCGCGGGCATCGCGCATGCCGCAATCGGACATAATCGCGGTTTGCACGCTGTTTGCCATTCCGC
CTATCCGCAATGTCCGATTC

Product: nitrogen metabolism transcriptional regulator, NtrC, Fis family

Products: NADP; 2 glutathione [C]

Alternate protein names: NA

Number of amino acids: Translated: 506; Mature: 506

Protein sequence:

>506_residues
MKPIWIVDDDQSIRWVLEKALARDSFATKSFANVRDALAALDHETPQVLVSDIRMPGGSGLELLQAMHERLPGLPVIIMT
AFSDLDSAVAAFQGGAFEYLAKPFDVDKAVELIRRAVEESLRGGAPQDERVAEAPEMLGQAPAMQDMFRAIGRLSHSAAT
VLITGESGTGKELVARALHRHSPRANGPFIALNTAAIPKDLLESELFGHERGAFTGAQTTRQGRFEQAENGTLFLDEIGD
MPFDLQTRLLRVLSDGQFYRVGGHNPLRANVRVIAATHQNLESRVRQGLFREDLYHRLNVIRLRLPPLRERSEDIALLTR
HFLQKSARDLGVEPKRVSDEALVYLTSLAFPGNVRQLENLANWLTVMAPAQTVEIKDLPPDLVPAGAPVVATSDGVDAHG
GSGEAATLAQPAIGGAPLASAPVAAAPNGSAPAGYPLWEHGLRTEVARLLRENSADVMDELARRFEAAVIREALDFTRGR
KVEAAERLGIGRNTITRKIQELHLEP

Sequences:

>Translated_506_residues
MKPIWIVDDDQSIRWVLEKALARDSFATKSFANVRDALAALDHETPQVLVSDIRMPGGSGLELLQAMHERLPGLPVIIMT
AFSDLDSAVAAFQGGAFEYLAKPFDVDKAVELIRRAVEESLRGGAPQDERVAEAPEMLGQAPAMQDMFRAIGRLSHSAAT
VLITGESGTGKELVARALHRHSPRANGPFIALNTAAIPKDLLESELFGHERGAFTGAQTTRQGRFEQAENGTLFLDEIGD
MPFDLQTRLLRVLSDGQFYRVGGHNPLRANVRVIAATHQNLESRVRQGLFREDLYHRLNVIRLRLPPLRERSEDIALLTR
HFLQKSARDLGVEPKRVSDEALVYLTSLAFPGNVRQLENLANWLTVMAPAQTVEIKDLPPDLVPAGAPVVATSDGVDAHG
GSGEAATLAQPAIGGAPLASAPVAAAPNGSAPAGYPLWEHGLRTEVARLLRENSADVMDELARRFEAAVIREALDFTRGR
KVEAAERLGIGRNTITRKIQELHLEP
>Mature_506_residues
MKPIWIVDDDQSIRWVLEKALARDSFATKSFANVRDALAALDHETPQVLVSDIRMPGGSGLELLQAMHERLPGLPVIIMT
AFSDLDSAVAAFQGGAFEYLAKPFDVDKAVELIRRAVEESLRGGAPQDERVAEAPEMLGQAPAMQDMFRAIGRLSHSAAT
VLITGESGTGKELVARALHRHSPRANGPFIALNTAAIPKDLLESELFGHERGAFTGAQTTRQGRFEQAENGTLFLDEIGD
MPFDLQTRLLRVLSDGQFYRVGGHNPLRANVRVIAATHQNLESRVRQGLFREDLYHRLNVIRLRLPPLRERSEDIALLTR
HFLQKSARDLGVEPKRVSDEALVYLTSLAFPGNVRQLENLANWLTVMAPAQTVEIKDLPPDLVPAGAPVVATSDGVDAHG
GSGEAATLAQPAIGGAPLASAPVAAAPNGSAPAGYPLWEHGLRTEVARLLRENSADVMDELARRFEAAVIREALDFTRGR
KVEAAERLGIGRNTITRKIQELHLEP

Specific function: Member of the two-component regulatory system ntrB/ntrC involved in the activation of nitrogen assimilatory genes such as glnA. NtrC is phosphorylated by ntrB and interacts with sigma-54 [H]

COG id: COG2204

COG function: function code T; Response regulator containing CheY-like receiver, AAA-type ATPase, and DNA-binding domains

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 sigma-54 factor interaction domain [H]

Homologues:

Organism=Escherichia coli, GI1790299, Length=503, Percent_Identity=55.8648111332008, Blast_Score=529, Evalue=1e-151,
Organism=Escherichia coli, GI1788550, Length=508, Percent_Identity=38.9763779527559, Blast_Score=354, Evalue=8e-99,
Organism=Escherichia coli, GI1790437, Length=497, Percent_Identity=37.2233400402414, Blast_Score=290, Evalue=2e-79,
Organism=Escherichia coli, GI1788905, Length=371, Percent_Identity=41.5094339622642, Blast_Score=278, Evalue=4e-76,
Organism=Escherichia coli, GI87082117, Length=225, Percent_Identity=53.3333333333333, Blast_Score=240, Evalue=1e-64,
Organism=Escherichia coli, GI1789233, Length=382, Percent_Identity=38.2198952879581, Blast_Score=233, Evalue=2e-62,
Organism=Escherichia coli, GI1789087, Length=286, Percent_Identity=45.1048951048951, Blast_Score=233, Evalue=2e-62,
Organism=Escherichia coli, GI87082152, Length=235, Percent_Identity=48.936170212766, Blast_Score=220, Evalue=2e-58,
Organism=Escherichia coli, GI1786524, Length=262, Percent_Identity=46.9465648854962, Blast_Score=213, Evalue=2e-56,
Organism=Escherichia coli, GI1787583, Length=253, Percent_Identity=44.2687747035573, Blast_Score=201, Evalue=7e-53,
Organism=Escherichia coli, GI87081872, Length=227, Percent_Identity=45.8149779735683, Blast_Score=193, Evalue=2e-50,
Organism=Escherichia coli, GI1789828, Length=226, Percent_Identity=35.8407079646018, Blast_Score=123, Evalue=3e-29,
Organism=Escherichia coli, GI87081858, Length=228, Percent_Identity=33.7719298245614, Blast_Score=120, Evalue=2e-28,
Organism=Escherichia coli, GI1789809, Length=174, Percent_Identity=27.0114942528736, Blast_Score=64, Evalue=2e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003593
- InterPro:   IPR011006
- InterPro:   IPR020441
- InterPro:   IPR009057
- InterPro:   IPR002197
- InterPro:   IPR002078
- InterPro:   IPR010114
- InterPro:   IPR001789 [H]

Pfam domain/function: PF02954 HTH_8; PF00072 Response_reg; PF00158 Sigma54_activat [H]

EC number: NA

Molecular weight: Translated: 55166; Mature: 55166

Theoretical pI: Translated: 6.08; Mature: 6.08

Prosite motif: PS50110 RESPONSE_REGULATORY ; PS00675 SIGMA54_INTERACT_1 ; PS00676 SIGMA54_INTERACT_2 ; PS00688 SIGMA54_INTERACT_3 ; PS50045 SIGMA54_INTERACT_4

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
2.0 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
2.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKPIWIVDDDQSIRWVLEKALARDSFATKSFANVRDALAALDHETPQVLVSDIRMPGGSG
CCCEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHCCCCCCC
LELLQAMHERLPGLPVIIMTAFSDLDSAVAAFQGGAFEYLAKPFDVDKAVELIRRAVEES
HHHHHHHHHHCCCCCEEEHHHHHHHHHHHHHHCCCCHHHHCCCCCHHHHHHHHHHHHHHH
LRGGAPQDERVAEAPEMLGQAPAMQDMFRAIGRLSHSAATVLITGESGTGKELVARALHR
HCCCCCCHHHHHHHHHHHCCCCCHHHHHHHHHHHCCCCEEEEEECCCCCCHHHHHHHHHH
HSPRANGPFIALNTAAIPKDLLESELFGHERGAFTGAQTTRQGRFEQAENGTLFLDEIGD
CCCCCCCCEEEEECCCCCHHHHHHHHHCCCCCCCCCCCHHHCCCCHHCCCCCEEEHHHCC
MPFDLQTRLLRVLSDGQFYRVGGHNPLRANVRVIAATHQNLESRVRQGLFREDLYHRLNV
CCHHHHHHHHHHHCCCCEEEECCCCCCCCCEEEEEEHHHHHHHHHHHHHHHHHHHHHHHE
IRLRLPPLRERSEDIALLTRHFLQKSARDLGVEPKRVSDEALVYLTSLAFPGNVRQLENL
EEEECCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHCCCCCHHHHHHH
ANWLTVMAPAQTVEIKDLPPDLVPAGAPVVATSDGVDAHGGSGEAATLAQPAIGGAPLAS
HHHHHHHCCCCEEEECCCCCCCCCCCCCEEEECCCCCCCCCCCCCHHHHCCCCCCCCCCC
APVAAAPNGSAPAGYPLWEHGLRTEVARLLRENSADVMDELARRFEAAVIREALDFTRGR
CCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHCCC
KVEAAERLGIGRNTITRKIQELHLEP
CCHHHHHHCCCHHHHHHHHHHHCCCC
>Mature Secondary Structure
MKPIWIVDDDQSIRWVLEKALARDSFATKSFANVRDALAALDHETPQVLVSDIRMPGGSG
CCCEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHCCCCCCC
LELLQAMHERLPGLPVIIMTAFSDLDSAVAAFQGGAFEYLAKPFDVDKAVELIRRAVEES
HHHHHHHHHHCCCCCEEEHHHHHHHHHHHHHHCCCCHHHHCCCCCHHHHHHHHHHHHHHH
LRGGAPQDERVAEAPEMLGQAPAMQDMFRAIGRLSHSAATVLITGESGTGKELVARALHR
HCCCCCCHHHHHHHHHHHCCCCCHHHHHHHHHHHCCCCEEEEEECCCCCCHHHHHHHHHH
HSPRANGPFIALNTAAIPKDLLESELFGHERGAFTGAQTTRQGRFEQAENGTLFLDEIGD
CCCCCCCCEEEEECCCCCHHHHHHHHHCCCCCCCCCCCHHHCCCCHHCCCCCEEEHHHCC
MPFDLQTRLLRVLSDGQFYRVGGHNPLRANVRVIAATHQNLESRVRQGLFREDLYHRLNV
CCHHHHHHHHHHHCCCCEEEECCCCCCCCCEEEEEEHHHHHHHHHHHHHHHHHHHHHHHE
IRLRLPPLRERSEDIALLTRHFLQKSARDLGVEPKRVSDEALVYLTSLAFPGNVRQLENL
EEEECCCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHCCCCCHHHHHHH
ANWLTVMAPAQTVEIKDLPPDLVPAGAPVVATSDGVDAHGGSGEAATLAQPAIGGAPLAS
HHHHHHHCCCCEEEECCCCCCCCCCCCCEEEECCCCCCCCCCCCCHHHHCCCCCCCCCCC
APVAAAPNGSAPAGYPLWEHGLRTEVARLLRENSADVMDELARRFEAAVIREALDFTRGR
CCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHCCC
KVEAAERLGIGRNTITRKIQELHLEP
CCHHHHHHCCCHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NADPH2; oxidized glutathione [C]

Specific reaction: NADPH2 + oxidized glutathione = NADP + 2 glutathione [C]

General reaction: Oxidoreductases; Acting on NADH or NADPH; With a disulfide as acceptor [C]

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]