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The map label for this gene is ptrB [C]

Identifier: 78066924

GI number: 78066924

Start: 2532190

End: 2534304

Strand: Reverse

Name: ptrB [C]

Synonym: Bcep18194_A5455

Alternate gene names: 78066924

Gene position: 2534304-2532190 (Counterclockwise)

Preceding gene: 78066925

Following gene: 78066922

Centisome position: 68.6

GC content: 70.02

Gene sequence:

>2115_bases
ATGTCCGATTCCTTCCGCTGGCCCGCTGGGGCCGACCCGTTCCGTTTCCTCGAATCGCTCGACAGCAAGCGCGCCCGCAC
CTGGGTCGACGAGCAGAATGCGCGCACGCGCGCCGCGCTGCGTGACGACGACGCGTATCGCGCGCTGACCGCGCGCCTCG
CCAAAGCGTATCTGCCGCGCGAACGCCCGGTGATTCCGACCCGCTGGCGCGACTGGGCCTACGACCTGTGGCAGGACGAT
CTCCATCCGAAAGGGCTGTGGCGCCGCACGCGCTGGGACGACTGGCGCGCGGGAAACCCGGCGTGGGAAACGCTGCTCGA
CGTCGACGCGCTTGGTGCGGAGGAGGGCGAGTCGTGGGTGTTCGAGCAGGACTCGATCCTGTATCCGGACGGTGATCGGG
CGCTGCTGTCGCTGTCGCCGGGCGGCGCCGACGCGGTCGTCGTGCGTGAATTCGATCTCGTCGAACGGCGTTTCGTCGAC
GGCGGCTTCACGATCGACGAGCCGGGGCATCACACGGTCGGCTGGATCGATCGCGATACCGTCTACGTGAGCTGGGATCG
CGGCGAAGCGCATGCGACCGCGGCCGGCTATCCGTATGAAGCGCGGCGCTGGGTGCGCGGCACGGCGCTCGCCGATGCGC
CCGTCGTGTTCAGCGGCGAACCCGACGACATCAGTGCGGGTGCGGGGTTCGATCCGATCGACAACCGTCACGTCGCGTGG
CGCAGCGTCGATTTCTTCGACGCACATGCGTACCGGCTGACCGACACGGGCGAGTGGGCGCGCTACGACGTGCCGACCCA
TGTCGAGGTCGGGTTCTGGGAGGGCTGGCTCATGCTGGAGCCGCGCCTCGACTGGGATTGTGACGGCGTGCGCCATGCGG
GCGGTTCGCTGCTCGCGATCCGCGAGCAGGCGTTTCTCGCCGGGTCGCGCGCACTCACGACGCTGTTCGTGCCGCAACCG
ACGACATCCGCGTGCACGTGGACGCACACGCGCACGACGCTGATCGCGAGCTGGCTCGACGACGTGCACAACCGCACGAT
GCTGTGGCAGCCGAGCCAGGCCGACGACGGTACGTGGACGTGGGATGCCCGGCCGTTCGAGTGGCCGGGGCCGGGCGACG
CGCAGATCGACGTCGAGCCGGTCGAAGCGACGCTGAACGACGAGATTTACGTGGACGTCGACACCTATCTCGATCCGCCC
GAATGCTGGCTGGCCGATCTTGCCGATCGCGCGGACGACGCGCCGTCGCGTCGCGTGCTGCTCGACCGGCCGCCGGTGCA
GTTCGATGCGGCCGGGCTGGTCGTGCGTCGTGCGAGCGCGCGCTCGCAAGACGGCACGATCGTGCCGTACACGCTGATCG
GGCCACGCGATGCACTCGACGTGGCCGACGGCGCCGCGCGCGTTGCGCGGCCGTGCCTGCTATCGGGCTATGGCGGTTTT
GCGATTCCGAACCTGCCGGGCTACAGCGATGCGTTCGGTATCGGGTGGCTCGAGCGCGGCGGCGTGATGGCGTTCGCGCA
CATCCGCGGCGGCGGCGAGTTCGGGCCGCGCTGGCACGTCGATGCGCAGCGCGAACACCGCCAGCGTTCCTTCGACGATT
TCATCGCGGTGGCCGAGGATCTGGCCGCGACCGGCGTGACGACCGCCGCGCAGCTCGGCATCGAGGGGGGCAGCAACGGC
GGGTTGCTGGTCGCCGCGTGCATGGTGCAGCGGCCGGAGCTGTTCGGCGCGGTGCTCTGCCGCGTGCCGCTGCTCGACAT
GCGGCGTTATCCGAAGCTGCACGCGGGCGCCGCATGGCTCGACGAATACGGCGATCCGGACGATCCGCATGAAGGCGCGG
CGCTGGCCGCGTACTCGCCGTATCACCGCGTGCGCGAAGGTGTCGCGTATCCGCCGCTGCTGCTGACGACGTCGACGCGC
GACGACCGTGTGCATCCCGCGCATGCGCGCAAGATGGCCGCGCGCATGCATGCGCTCGGTCACGAACGGGTGTGGTACTG
GGAGAACACCGACGGTGGCCACGGCAGCGCCGACGATCTGGAACGCGCGGAAGCCGATGCGGCCGAATTCGGGTTCCTGT
GGGCCCATCTCGGGCCGGCGCCCGCGCGGCGCTGA

Upstream 100 bases:

>100_bases
TGGAGCCCTGAGCACGATTTGCGGCATGCGCGGGCATCGCGCATGCCGCAATCGGACATAATCGCGGTTTGCACGCTGTT
TGCCATTCCGCCTATCCGCA

Downstream 100 bases:

>100_bases
ACGCGCGCGGGCGGATCGAGCGATCAGCCGACGACCGCGACGACGGGCGTGTGGTCGGACGGCTGTTCCCACGTGCGCGG
CGTGCGATCGACTTCGCACG

Product: peptidase S9, prolyl oligopeptidase active site region

Products: Hydrolyzed protein [C]

Alternate protein names: NA

Number of amino acids: Translated: 704; Mature: 703

Protein sequence:

>704_residues
MSDSFRWPAGADPFRFLESLDSKRARTWVDEQNARTRAALRDDDAYRALTARLAKAYLPRERPVIPTRWRDWAYDLWQDD
LHPKGLWRRTRWDDWRAGNPAWETLLDVDALGAEEGESWVFEQDSILYPDGDRALLSLSPGGADAVVVREFDLVERRFVD
GGFTIDEPGHHTVGWIDRDTVYVSWDRGEAHATAAGYPYEARRWVRGTALADAPVVFSGEPDDISAGAGFDPIDNRHVAW
RSVDFFDAHAYRLTDTGEWARYDVPTHVEVGFWEGWLMLEPRLDWDCDGVRHAGGSLLAIREQAFLAGSRALTTLFVPQP
TTSACTWTHTRTTLIASWLDDVHNRTMLWQPSQADDGTWTWDARPFEWPGPGDAQIDVEPVEATLNDEIYVDVDTYLDPP
ECWLADLADRADDAPSRRVLLDRPPVQFDAAGLVVRRASARSQDGTIVPYTLIGPRDALDVADGAARVARPCLLSGYGGF
AIPNLPGYSDAFGIGWLERGGVMAFAHIRGGGEFGPRWHVDAQREHRQRSFDDFIAVAEDLAATGVTTAAQLGIEGGSNG
GLLVAACMVQRPELFGAVLCRVPLLDMRRYPKLHAGAAWLDEYGDPDDPHEGAALAAYSPYHRVREGVAYPPLLLTTSTR
DDRVHPAHARKMAARMHALGHERVWYWENTDGGHGSADDLERAEADAAEFGFLWAHLGPAPARR

Sequences:

>Translated_704_residues
MSDSFRWPAGADPFRFLESLDSKRARTWVDEQNARTRAALRDDDAYRALTARLAKAYLPRERPVIPTRWRDWAYDLWQDD
LHPKGLWRRTRWDDWRAGNPAWETLLDVDALGAEEGESWVFEQDSILYPDGDRALLSLSPGGADAVVVREFDLVERRFVD
GGFTIDEPGHHTVGWIDRDTVYVSWDRGEAHATAAGYPYEARRWVRGTALADAPVVFSGEPDDISAGAGFDPIDNRHVAW
RSVDFFDAHAYRLTDTGEWARYDVPTHVEVGFWEGWLMLEPRLDWDCDGVRHAGGSLLAIREQAFLAGSRALTTLFVPQP
TTSACTWTHTRTTLIASWLDDVHNRTMLWQPSQADDGTWTWDARPFEWPGPGDAQIDVEPVEATLNDEIYVDVDTYLDPP
ECWLADLADRADDAPSRRVLLDRPPVQFDAAGLVVRRASARSQDGTIVPYTLIGPRDALDVADGAARVARPCLLSGYGGF
AIPNLPGYSDAFGIGWLERGGVMAFAHIRGGGEFGPRWHVDAQREHRQRSFDDFIAVAEDLAATGVTTAAQLGIEGGSNG
GLLVAACMVQRPELFGAVLCRVPLLDMRRYPKLHAGAAWLDEYGDPDDPHEGAALAAYSPYHRVREGVAYPPLLLTTSTR
DDRVHPAHARKMAARMHALGHERVWYWENTDGGHGSADDLERAEADAAEFGFLWAHLGPAPARR
>Mature_703_residues
SDSFRWPAGADPFRFLESLDSKRARTWVDEQNARTRAALRDDDAYRALTARLAKAYLPRERPVIPTRWRDWAYDLWQDDL
HPKGLWRRTRWDDWRAGNPAWETLLDVDALGAEEGESWVFEQDSILYPDGDRALLSLSPGGADAVVVREFDLVERRFVDG
GFTIDEPGHHTVGWIDRDTVYVSWDRGEAHATAAGYPYEARRWVRGTALADAPVVFSGEPDDISAGAGFDPIDNRHVAWR
SVDFFDAHAYRLTDTGEWARYDVPTHVEVGFWEGWLMLEPRLDWDCDGVRHAGGSLLAIREQAFLAGSRALTTLFVPQPT
TSACTWTHTRTTLIASWLDDVHNRTMLWQPSQADDGTWTWDARPFEWPGPGDAQIDVEPVEATLNDEIYVDVDTYLDPPE
CWLADLADRADDAPSRRVLLDRPPVQFDAAGLVVRRASARSQDGTIVPYTLIGPRDALDVADGAARVARPCLLSGYGGFA
IPNLPGYSDAFGIGWLERGGVMAFAHIRGGGEFGPRWHVDAQREHRQRSFDDFIAVAEDLAATGVTTAAQLGIEGGSNGG
LLVAACMVQRPELFGAVLCRVPLLDMRRYPKLHAGAAWLDEYGDPDDPHEGAALAAYSPYHRVREGVAYPPLLLTTSTRD
DRVHPAHARKMAARMHALGHERVWYWENTDGGHGSADDLERAEADAAEFGFLWAHLGPAPARR

Specific function: Cleaves Peptide Bonds On The C-Terminal Side Of Lysyl And Argininyl Residues. [C]

COG id: COG1505

COG function: function code E; Serine proteases of the peptidase family S9A

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the peptidase S9B family [H]

Homologues:

Organism=Homo sapiens, GI41349456, Length=317, Percent_Identity=35.0157728706625, Blast_Score=174, Evalue=2e-43,
Organism=Homo sapiens, GI284172438, Length=215, Percent_Identity=27.906976744186, Blast_Score=81, Evalue=4e-15,
Organism=Homo sapiens, GI284172431, Length=215, Percent_Identity=27.906976744186, Blast_Score=81, Evalue=4e-15,
Organism=Homo sapiens, GI284172420, Length=215, Percent_Identity=27.906976744186, Blast_Score=80, Evalue=5e-15,
Organism=Homo sapiens, GI284172413, Length=215, Percent_Identity=27.906976744186, Blast_Score=80, Evalue=5e-15,
Organism=Homo sapiens, GI70778815, Length=215, Percent_Identity=27.906976744186, Blast_Score=80, Evalue=5e-15,
Organism=Homo sapiens, GI108860686, Length=215, Percent_Identity=27.906976744186, Blast_Score=80, Evalue=5e-15,
Organism=Homo sapiens, GI108860692, Length=212, Percent_Identity=27.8301886792453, Blast_Score=75, Evalue=3e-13,
Organism=Escherichia coli, GI1788150, Length=289, Percent_Identity=29.757785467128, Blast_Score=118, Evalue=1e-27,
Organism=Drosophila melanogaster, GI24583414, Length=278, Percent_Identity=37.410071942446, Blast_Score=180, Evalue=3e-45,
Organism=Drosophila melanogaster, GI221510989, Length=282, Percent_Identity=36.8794326241135, Blast_Score=172, Evalue=1e-42,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001375
- InterPro:   IPR002470
- InterPro:   IPR004106 [H]

Pfam domain/function: PF00326 Peptidase_S9; PF02897 Peptidase_S9_N [H]

EC number: 3.4.21.83 [C]

Molecular weight: Translated: 78523; Mature: 78391

Theoretical pI: Translated: 4.69; Mature: 4.69

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
1.1 %Met     (Translated Protein)
2.0 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
1.0 %Met     (Mature Protein)
1.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSDSFRWPAGADPFRFLESLDSKRARTWVDEQNARTRAALRDDDAYRALTARLAKAYLPR
CCCCCCCCCCCCHHHHHHHHHHHHHHHHCCCCCCHHHHHHCCCHHHHHHHHHHHHHHCCC
ERPVIPTRWRDWAYDLWQDDLHPKGLWRRTRWDDWRAGNPAWETLLDVDALGAEEGESWV
CCCCCCCCCCHHHHHHHHCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHCCCCCCCCEE
FEQDSILYPDGDRALLSLSPGGADAVVVREFDLVERRFVDGGFTIDEPGHHTVGWIDRDT
EECCCEECCCCCCEEEEECCCCCCEEEEEHHHHHHHHHCCCCCEECCCCCCEEEEEECCE
VYVSWDRGEAHATAAGYPYEARRWVRGTALADAPVVFSGEPDDISAGAGFDPIDNRHVAW
EEEEECCCCCCEEECCCCHHHHHHHCCCEECCCCEEECCCCCCCCCCCCCCCCCCCEEEE
RSVDFFDAHAYRLTDTGEWARYDVPTHVEVGFWEGWLMLEPRLDWDCDGVRHAGGSLLAI
EECEEECCCEEEECCCCCCEEECCCCEEEEEEECCEEEECCCCCCCCCCHHHCCCCEEEE
REQAFLAGSRALTTLFVPQPTTSACTWTHTRTTLIASWLDDVHNRTMLWQPSQADDGTWT
EHHHHHCCCCEEEEEEECCCCCCCEEEECHHHHHHHHHHHHHHCCEEEECCCCCCCCEEE
WDARPFEWPGPGDAQIDVEPVEATLNDEIYVDVDTYLDPPECWLADLADRADDAPSRRVL
ECCCCCCCCCCCCCEEEEEEEEEECCCEEEEEECCCCCCHHHHHHHHHHHCCCCCCCEEE
LDRPPVQFDAAGLVVRRASARSQDGTIVPYTLIGPRDALDVADGAARVARPCLLSGYGGF
ECCCCCCCCCCCEEEEECCCCCCCCCEEEEEEECCCCHHHHCCCHHHHHCCCEEECCCCC
AIPNLPGYSDAFGIGWLERGGVMAFAHIRGGGEFGPRWHVDAQREHRQRSFDDFIAVAED
CCCCCCCCCCCCCCCEEECCCEEEEEEECCCCCCCCEECCCCHHHHHHHHHHHHHHHHHH
LAATGVTTAAQLGIEGGSNGGLLVAACMVQRPELFGAVLCRVPLLDMRRYPKLHAGAAWL
HHHCCCCHHHHCCCCCCCCCCEEEEEHHHCCHHHHHHHHHHCCHHHHHCCCCHHCCHHHH
DEYGDPDDPHEGAALAAYSPYHRVREGVAYPPLLLTTSTRDDRVHPAHARKMAARMHALG
HHCCCCCCCCCCCEEEEECHHHHHHCCCCCCCEEEEECCCCCCCCHHHHHHHHHHHHHCC
HERVWYWENTDGGHGSADDLERAEADAAEFGFLWAHLGPAPARR
CCEEEEEECCCCCCCCHHHHHHHHCCHHHHCEEEEECCCCCCCC
>Mature Secondary Structure 
SDSFRWPAGADPFRFLESLDSKRARTWVDEQNARTRAALRDDDAYRALTARLAKAYLPR
CCCCCCCCCCCHHHHHHHHHHHHHHHHCCCCCCHHHHHHCCCHHHHHHHHHHHHHHCCC
ERPVIPTRWRDWAYDLWQDDLHPKGLWRRTRWDDWRAGNPAWETLLDVDALGAEEGESWV
CCCCCCCCCCHHHHHHHHCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHCCCCCCCCEE
FEQDSILYPDGDRALLSLSPGGADAVVVREFDLVERRFVDGGFTIDEPGHHTVGWIDRDT
EECCCEECCCCCCEEEEECCCCCCEEEEEHHHHHHHHHCCCCCEECCCCCCEEEEEECCE
VYVSWDRGEAHATAAGYPYEARRWVRGTALADAPVVFSGEPDDISAGAGFDPIDNRHVAW
EEEEECCCCCCEEECCCCHHHHHHHCCCEECCCCEEECCCCCCCCCCCCCCCCCCCEEEE
RSVDFFDAHAYRLTDTGEWARYDVPTHVEVGFWEGWLMLEPRLDWDCDGVRHAGGSLLAI
EECEEECCCEEEECCCCCCEEECCCCEEEEEEECCEEEECCCCCCCCCCHHHCCCCEEEE
REQAFLAGSRALTTLFVPQPTTSACTWTHTRTTLIASWLDDVHNRTMLWQPSQADDGTWT
EHHHHHCCCCEEEEEEECCCCCCCEEEECHHHHHHHHHHHHHHCCEEEECCCCCCCCEEE
WDARPFEWPGPGDAQIDVEPVEATLNDEIYVDVDTYLDPPECWLADLADRADDAPSRRVL
ECCCCCCCCCCCCCEEEEEEEEEECCCEEEEEECCCCCCHHHHHHHHHHHCCCCCCCEEE
LDRPPVQFDAAGLVVRRASARSQDGTIVPYTLIGPRDALDVADGAARVARPCLLSGYGGF
ECCCCCCCCCCCEEEEECCCCCCCCCEEEEEEECCCCHHHHCCCHHHHHCCCEEECCCCC
AIPNLPGYSDAFGIGWLERGGVMAFAHIRGGGEFGPRWHVDAQREHRQRSFDDFIAVAED
CCCCCCCCCCCCCCCEEECCCEEEEEEECCCCCCCCEECCCCHHHHHHHHHHHHHHHHHH
LAATGVTTAAQLGIEGGSNGGLLVAACMVQRPELFGAVLCRVPLLDMRRYPKLHAGAAWL
HHHCCCCHHHHCCCCCCCCCCEEEEEHHHCCHHHHHHHHHHCCHHHHHCCCCHHCCHHHH
DEYGDPDDPHEGAALAAYSPYHRVREGVAYPPLLLTTSTRDDRVHPAHARKMAARMHALG
HHCCCCCCCCCCCEEEEECHHHHHHCCCCCCCEEEEECCCCCCCCHHHHHHHHHHHHHCC
HERVWYWENTDGGHGSADDLERAEADAAEFGFLWAHLGPAPARR
CCEEEEEECCCCCCCCHHHHHHHHCCHHHHCEEEEECCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: Ca2+ [C]

Kcat value (1/min): 11820 [C]

Specific activity: NA

Km value (mM): 0.23 {tosyl-Arg} 0.92 {benzoyl-Lys} 0.6 {N-benzoyl-Arg} 0.47 {tosyl-Lys-methyl} 0.33 {N-benzyloxycarbonyl-Lys} 0.31 {N-benzyloxycarbonyl-Lys} 0.5 {benzoyl-Arg} 0.48 {benzoyl-Arg} 0.25 {benzoyl-Arg} 80 {acetyl-tyrosine} [C]

Substrates: Protein; H2O [C]

Specific reaction: Protein + H2O = hydrolyzed protein [C]

General reaction: Peptide bond hydrolysis [C]

Inhibitor: Antipain; Aromaticamidines; Benzamidine; Co2+; DFP; Fe2+; Hg2+; L-Arginine; Leupeptin sulfhydryl agents, trypsin inhibitors, 1, 10-phenanthroline; p-Aminobenzamidine; Tosyl -Leuchloromethyl ketone; Zn2+ [C]

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9163424 [H]