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The map label for this gene is pdhB [H]

Identifier: 78066911

GI number: 78066911

Start: 2511583

End: 2513229

Strand: Reverse

Name: pdhB [H]

Synonym: Bcep18194_A5442

Alternate gene names: 78066911

Gene position: 2513229-2511583 (Counterclockwise)

Preceding gene: 78066912

Following gene: 78066910

Centisome position: 68.03

GC content: 66.55

Gene sequence:

>1647_bases
ATGAGTCAAGCGATCGAAGTGAAGGTGCCGGATATCGGCGATTACAAGGACGTCCCGGTGATCGAGATCGGCGTGAAGGT
CGGCGATACCGTCGAGCCGGAGCAGTCGCTCGTCACGCTCGAGTCGGACAAGGCGACGATGGACGTGCCGAGCCCGGTCG
GCGGCGTCGTGAAGGAAATCAAGGTCAAGGTGGGCGATTCCGTGTCGGAAGGCTCGCTGATCATCCTGCTCGAAGGCGGC
GCCGCGGCGCAGGCGAACGGCGCGGCAGCGCCCGCAGCTGCTCCGGCTCCGGCGGCAGCGCCGGCACCGGCTGCAGCAAC
CCCGGCGGCTGCCCCGGCAGCGACCGGTGGCACGCTCGAAGTGAAGGTCCCCGACATCGGCGATTACAAGGATGTGCCGG
TGATCGAGATCGGCGTGAAGGTTGGCGACACGGTCGAGAAGGAGCAGTCGCTCGTCACGCTCGAGTCCGACAAGGCGACG
ATGGACGTGCCGAGCCCGGCCGCCGGCGTCGTGAAAGAGATCAAGGTCAAGGTGGGCGATTCGGTGTCGGAAGGCACGCT
GATCGTGCTGCTCGACGCGGCAGGCGCAGCGCCGGCAGCAGCCGCACCGCAGGCGAGCGCACCGGCTCCGGCCGCTGCCG
CACCGGCACCTGCCGCCGCGCCCGCGAAGGCCGCAGCACCGGCTGCTGCTGCGCCGGCTCCGGCCGCTGCGCCGTCGGGT
GAATACCGCGCCAGCCACGCATCGCCGTCGGTGCGCAAGTTCGCGCGCGAACTCGGCGTCGAAGTCGCACGCGTGCAGGG
TTCGGGTCCGAAGGGCCGCATTACGAAGGAAGACGTCACGGGCTTCGTGAAGGGCGTGATGACCGGCCAGCGCACAGCAC
CGGGCGCGGCAGCCGCGCCGGCAGGCGGCGGTGAGCTGAACCTGCTGCCGTGGCCGAAGGTCGACTTCTCGAAGTTCGGC
CCGTTCGAGGCGAAGCCGCTGTCGCGCATCAAGAAGATCTCGGGCGCGAACCTGCATCGCAACTGGGTGATGATCCCGCA
CGTCACGAACAACGACGAAGCGGACATCACCGAGCTCGAAGCACTGCGCGTGCAGTTGAACAAGGAACACGAAAAGGCGG
GCGTGAAGTTCACGATGCTCGCGTTCGTGATCAAGGCGGTCGTCGCTGCGCTGAAGAAGTTCCCGACCTTCAACGCGAGC
CTCGACGGCGACAACCTGGTGTTCAAGCAGTACTACCACGTCGGTTTTGCTGCGGACACGCCGAACGGTCTCGTCGTGCC
GGTGATCCGCGATGCGGACAAGAAGGGGCTCGTCGATATCGCGAAGGAAATGGCCGAGCTGTCGAAGGCCGCACGCGACG
GCAAGCTGAAGCCGGACCAGATGCAAGGCGGCTGCTTCTCGATCTCGTCGCTCGGCGGGATCGGCGGCACGAACTTCACG
CCGATCATCAACGCGCCGGAAGTGGCGATCCTCGGGTTGTCGCGCGGCCAGATGAAGCCGGTGTGGGACGGCAAGCAGTT
CGTGCCGCGTCTGACGCTGCCGCTGTCGCTGTCGTATGACCATCGCGTGATCGATGGCGCGGAAGCCGCGCGGTTCAATG
CGTATCTCGGCGCGTTGCTCGCGGATTTCCGTCGCATCATTCTTTGA

Upstream 100 bases:

>100_bases
ATCCGTCGAAGCCCAACCCGATGACCGTTTAACGCACACGCCGTGTGATGCCGCGCGCCGCTCCCGCGAGGGGCGCGCGC
GGTCATGGAGACAGAAACAG

Downstream 100 bases:

>100_bases
TGAGCGGGGAGAGTGCTCGCGGGGGCGTGGGTTTTTTGTTTGTCATCCGCGCCCGGTCGTTGAACCTGTTTCGCTAACGG
TCTATTAGCGTCGCCCCTGC

Product: dihydrolipoamide acetyltransferase

Products: NA

Alternate protein names: Dihydrolipoamide acetyltransferase component of pyruvate dehydrogenase complex; E2 [H]

Number of amino acids: Translated: 548; Mature: 547

Protein sequence:

>548_residues
MSQAIEVKVPDIGDYKDVPVIEIGVKVGDTVEPEQSLVTLESDKATMDVPSPVGGVVKEIKVKVGDSVSEGSLIILLEGG
AAAQANGAAAPAAAPAPAAAPAPAAATPAAAPAATGGTLEVKVPDIGDYKDVPVIEIGVKVGDTVEKEQSLVTLESDKAT
MDVPSPAAGVVKEIKVKVGDSVSEGTLIVLLDAAGAAPAAAAPQASAPAPAAAAPAPAAAPAKAAAPAAAAPAPAAAPSG
EYRASHASPSVRKFARELGVEVARVQGSGPKGRITKEDVTGFVKGVMTGQRTAPGAAAAPAGGGELNLLPWPKVDFSKFG
PFEAKPLSRIKKISGANLHRNWVMIPHVTNNDEADITELEALRVQLNKEHEKAGVKFTMLAFVIKAVVAALKKFPTFNAS
LDGDNLVFKQYYHVGFAADTPNGLVVPVIRDADKKGLVDIAKEMAELSKAARDGKLKPDQMQGGCFSISSLGGIGGTNFT
PIINAPEVAILGLSRGQMKPVWDGKQFVPRLTLPLSLSYDHRVIDGAEAARFNAYLGALLADFRRIIL

Sequences:

>Translated_548_residues
MSQAIEVKVPDIGDYKDVPVIEIGVKVGDTVEPEQSLVTLESDKATMDVPSPVGGVVKEIKVKVGDSVSEGSLIILLEGG
AAAQANGAAAPAAAPAPAAAPAPAAATPAAAPAATGGTLEVKVPDIGDYKDVPVIEIGVKVGDTVEKEQSLVTLESDKAT
MDVPSPAAGVVKEIKVKVGDSVSEGTLIVLLDAAGAAPAAAAPQASAPAPAAAAPAPAAAPAKAAAPAAAAPAPAAAPSG
EYRASHASPSVRKFARELGVEVARVQGSGPKGRITKEDVTGFVKGVMTGQRTAPGAAAAPAGGGELNLLPWPKVDFSKFG
PFEAKPLSRIKKISGANLHRNWVMIPHVTNNDEADITELEALRVQLNKEHEKAGVKFTMLAFVIKAVVAALKKFPTFNAS
LDGDNLVFKQYYHVGFAADTPNGLVVPVIRDADKKGLVDIAKEMAELSKAARDGKLKPDQMQGGCFSISSLGGIGGTNFT
PIINAPEVAILGLSRGQMKPVWDGKQFVPRLTLPLSLSYDHRVIDGAEAARFNAYLGALLADFRRIIL
>Mature_547_residues
SQAIEVKVPDIGDYKDVPVIEIGVKVGDTVEPEQSLVTLESDKATMDVPSPVGGVVKEIKVKVGDSVSEGSLIILLEGGA
AAQANGAAAPAAAPAPAAAPAPAAATPAAAPAATGGTLEVKVPDIGDYKDVPVIEIGVKVGDTVEKEQSLVTLESDKATM
DVPSPAAGVVKEIKVKVGDSVSEGTLIVLLDAAGAAPAAAAPQASAPAPAAAAPAPAAAPAKAAAPAAAAPAPAAAPSGE
YRASHASPSVRKFARELGVEVARVQGSGPKGRITKEDVTGFVKGVMTGQRTAPGAAAAPAGGGELNLLPWPKVDFSKFGP
FEAKPLSRIKKISGANLHRNWVMIPHVTNNDEADITELEALRVQLNKEHEKAGVKFTMLAFVIKAVVAALKKFPTFNASL
DGDNLVFKQYYHVGFAADTPNGLVVPVIRDADKKGLVDIAKEMAELSKAARDGKLKPDQMQGGCFSISSLGGIGGTNFTP
IINAPEVAILGLSRGQMKPVWDGKQFVPRLTLPLSLSYDHRVIDGAEAARFNAYLGALLADFRRIIL

Specific function: The pyruvate dehydrogenase complex catalyzes the overall conversion of pyruvate to acetyl-CoA and CO(2). It contains multiple copies of three enzymatic components:pyruvate dehydrogenase (E1), dihydrolipoamide acetyltransferase (E2) and lipoamide dehydroge

COG id: COG0508

COG function: function code C; Pyruvate/2-oxoglutarate dehydrogenase complex, dihydrolipoamide acyltransferase (E2) component, and related enzymes

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 2 lipoyl-binding domains [H]

Homologues:

Organism=Homo sapiens, GI110671329, Length=449, Percent_Identity=29.3986636971047, Blast_Score=170, Evalue=4e-42,
Organism=Homo sapiens, GI31711992, Length=474, Percent_Identity=30.5907172995781, Blast_Score=167, Evalue=2e-41,
Organism=Homo sapiens, GI203098816, Length=477, Percent_Identity=27.4633123689727, Blast_Score=138, Evalue=2e-32,
Organism=Homo sapiens, GI203098753, Length=432, Percent_Identity=28.2407407407407, Blast_Score=132, Evalue=6e-31,
Organism=Homo sapiens, GI19923748, Length=227, Percent_Identity=33.920704845815, Blast_Score=121, Evalue=2e-27,
Organism=Homo sapiens, GI260898739, Length=153, Percent_Identity=37.2549019607843, Blast_Score=97, Evalue=3e-20,
Organism=Escherichia coli, GI1786305, Length=547, Percent_Identity=54.8446069469835, Blast_Score=507, Evalue=1e-145,
Organism=Escherichia coli, GI1786946, Length=433, Percent_Identity=30.2540415704388, Blast_Score=184, Evalue=1e-47,
Organism=Caenorhabditis elegans, GI17537937, Length=443, Percent_Identity=29.1196388261851, Blast_Score=172, Evalue=6e-43,
Organism=Caenorhabditis elegans, GI17560088, Length=447, Percent_Identity=31.3199105145414, Blast_Score=148, Evalue=9e-36,
Organism=Caenorhabditis elegans, GI25146366, Length=205, Percent_Identity=38.0487804878049, Blast_Score=130, Evalue=2e-30,
Organism=Caenorhabditis elegans, GI17538894, Length=306, Percent_Identity=32.3529411764706, Blast_Score=119, Evalue=5e-27,
Organism=Saccharomyces cerevisiae, GI6320352, Length=447, Percent_Identity=28.6353467561521, Blast_Score=173, Evalue=5e-44,
Organism=Saccharomyces cerevisiae, GI6324258, Length=432, Percent_Identity=29.6296296296296, Blast_Score=137, Evalue=5e-33,
Organism=Drosophila melanogaster, GI18859875, Length=427, Percent_Identity=32.7868852459016, Blast_Score=187, Evalue=2e-47,
Organism=Drosophila melanogaster, GI24582497, Length=232, Percent_Identity=32.3275862068966, Blast_Score=126, Evalue=3e-29,
Organism=Drosophila melanogaster, GI20129315, Length=232, Percent_Identity=32.3275862068966, Blast_Score=126, Evalue=5e-29,
Organism=Drosophila melanogaster, GI24645909, Length=207, Percent_Identity=34.7826086956522, Blast_Score=118, Evalue=9e-27,

Paralogues:

None

Copy number: 1120 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). 912 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 4,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003016
- InterPro:   IPR001078
- InterPro:   IPR006256
- InterPro:   IPR000089
- InterPro:   IPR023213
- InterPro:   IPR004167
- InterPro:   IPR011053 [H]

Pfam domain/function: PF00198 2-oxoacid_dh; PF00364 Biotin_lipoyl; PF02817 E3_binding [H]

EC number: =2.3.1.12 [H]

Molecular weight: Translated: 56241; Mature: 56110

Theoretical pI: Translated: 6.30; Mature: 6.30

Prosite motif: PS50968 BIOTINYL_LIPOYL ; PS00189 LIPOYL

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
1.8 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
1.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSQAIEVKVPDIGDYKDVPVIEIGVKVGDTVEPEQSLVTLESDKATMDVPSPVGGVVKEI
CCCEEEEECCCCCCCCCCCEEEEECCCCCCCCCCCCEEEEECCCCEECCCCCHHHHHHHH
KVKVGDSVSEGSLIILLEGGAAAQANGAAAPAAAPAPAAAPAPAAATPAAAPAATGGTLE
HHHCCCCCCCCCEEEEECCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCEEE
VKVPDIGDYKDVPVIEIGVKVGDTVEKEQSLVTLESDKATMDVPSPAAGVVKEIKVKVGD
EECCCCCCCCCCCEEEECCCCCCCHHCCCCEEEEECCCCEECCCCCHHHHHHHHHHHCCC
SVSEGTLIVLLDAAGAAPAAAAPQASAPAPAAAAPAPAAAPAKAAAPAAAAPAPAAAPSG
CCCCCEEEEEEECCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHCCCCCCCCCCCCCCCC
EYRASHASPSVRKFARELGVEVARVQGSGPKGRITKEDVTGFVKGVMTGQRTAPGAAAAP
CCCCCCCCHHHHHHHHHHCCEEEEEECCCCCCCCCHHHHHHHHHHHHCCCCCCCCCCCCC
AGGGELNLLPWPKVDFSKFGPFEAKPLSRIKKISGANLHRNWVMIPHVTNNDEADITELE
CCCCEEEECCCCCCCHHHCCCCCCCHHHHHHHHCCCCEECCEEEEEEECCCCCCCHHHHH
ALRVQLNKEHEKAGVKFTMLAFVIKAVVAALKKFPTFNASLDGDNLVFKQYYHVGFAADT
HHHHHHCCHHHHCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCCCEEEEEEEEEEEECCC
PNGLVVPVIRDADKKGLVDIAKEMAELSKAARDGKLKPDQMQGGCFSISSLGGIGGTNFT
CCCEEEEEEECCCCCCHHHHHHHHHHHHHHHHCCCCCCHHHCCCEEEECCCCCCCCCCCC
PIINAPEVAILGLSRGQMKPVWDGKQFVPRLTLPLSLSYDHRVIDGAEAARFNAYLGALL
EEECCCCEEEEECCCCCCCCCCCCHHHCCEEEEEEEECCCCEEECCCHHHHHHHHHHHHH
ADFRRIIL
HHHHHHHC
>Mature Secondary Structure 
SQAIEVKVPDIGDYKDVPVIEIGVKVGDTVEPEQSLVTLESDKATMDVPSPVGGVVKEI
CCEEEEECCCCCCCCCCCEEEEECCCCCCCCCCCCEEEEECCCCEECCCCCHHHHHHHH
KVKVGDSVSEGSLIILLEGGAAAQANGAAAPAAAPAPAAAPAPAAATPAAAPAATGGTLE
HHHCCCCCCCCCEEEEECCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCEEE
VKVPDIGDYKDVPVIEIGVKVGDTVEKEQSLVTLESDKATMDVPSPAAGVVKEIKVKVGD
EECCCCCCCCCCCEEEECCCCCCCHHCCCCEEEEECCCCEECCCCCHHHHHHHHHHHCCC
SVSEGTLIVLLDAAGAAPAAAAPQASAPAPAAAAPAPAAAPAKAAAPAAAAPAPAAAPSG
CCCCCEEEEEEECCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHCCCCCCCCCCCCCCCC
EYRASHASPSVRKFARELGVEVARVQGSGPKGRITKEDVTGFVKGVMTGQRTAPGAAAAP
CCCCCCCCHHHHHHHHHHCCEEEEEECCCCCCCCCHHHHHHHHHHHHCCCCCCCCCCCCC
AGGGELNLLPWPKVDFSKFGPFEAKPLSRIKKISGANLHRNWVMIPHVTNNDEADITELE
CCCCEEEECCCCCCCHHHCCCCCCCHHHHHHHHCCCCEECCEEEEEEECCCCCCCHHHHH
ALRVQLNKEHEKAGVKFTMLAFVIKAVVAALKKFPTFNASLDGDNLVFKQYYHVGFAADT
HHHHHHCCHHHHCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCCCEEEEEEEEEEEECCC
PNGLVVPVIRDADKKGLVDIAKEMAELSKAARDGKLKPDQMQGGCFSISSLGGIGGTNFT
CCCEEEEEEECCCCCCHHHHHHHHHHHHHHHHCCCCCCHHHCCCEEEECCCCCCCCCCCC
PIINAPEVAILGLSRGQMKPVWDGKQFVPRLTLPLSLSYDHRVIDGAEAARFNAYLGALL
EEECCCCEEEEECCCCCCCCCCCCHHHCCEEEEEEEECCCCEEECCCHHHHHHHHHHHHH
ADFRRIIL
HHHHHHHC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8021225 [H]