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The map label for this gene is def

Identifier: 78066802

GI number: 78066802

Start: 2387917

End: 2388450

Strand: Reverse

Name: def

Synonym: Bcep18194_A5333

Alternate gene names: 78066802

Gene position: 2388450-2387917 (Counterclockwise)

Preceding gene: 78066803

Following gene: 78066798

Centisome position: 64.66

GC content: 64.23

Gene sequence:

>534_bases
ATGATTCGCGAGATCCTGAAGATGGGCGATCCGCGCCTGCTCGAAGTCGCCAAGCCGGTCGAGCGGTTCGATACCCCCGA
ACTGCACGAGATCGTCGCGGACATGTTCGAGACGATGCACCATGCGAACGGCGCCGGGCTGGCCGCGCCGCAGATCGGCA
TCGGGCTGCAGCTCATCATCTTCGGCTTCGGCAACAACAACCGCTACCCGGATGCGCCGCCGGTACCGGAGACCGTGCTG
ATCAACCCGAAGGTCGAGTACATGCCGCCGGACATGGAAGAGGGCTGGGAGGGCTGCCTGTCGGTGCCGGGCATGCGCGG
CGTCGTCAGCCGCTACGCGAAGGTGCGTTACAGCGGCTTCGACCAGTACGGCGAGAAGATCGATCGCGTCGCCGACGGTT
TTCACGCGCGCGTCGTCCAGCACGAATACGATCACCTGATCGGCAAGCTGTATCCGATGCGGATCACCGACTTCACGCGC
TTCGGTTTCACGGAAGTGCTGTTCCCGGGGCTCGATCCGGCGTCCGACGACTGA

Upstream 100 bases:

>100_bases
CGGGCGCCGAAGCCGGCAGCAAGCTCGTGAAGGCCGAGGAACTCGGCATCCCCGTGCTGGACGAAGACGGTCTGCACCAA
CTCCTGGAGGGCAATACGCC

Downstream 100 bases:

>100_bases
TCGCCCGGCGTTCCAGGTCTCTCGCAGGAAACAAAAAAGCCCGCTTGCGCGGGCTTTTTCATTGCGTGGCGCACGTGTGT
GCGCTCGCGGTCAGAACGTC

Product: peptide deformylase

Products: NA

Alternate protein names: PDF 2; Polypeptide deformylase 2 [H]

Number of amino acids: Translated: 177; Mature: 177

Protein sequence:

>177_residues
MIREILKMGDPRLLEVAKPVERFDTPELHEIVADMFETMHHANGAGLAAPQIGIGLQLIIFGFGNNNRYPDAPPVPETVL
INPKVEYMPPDMEEGWEGCLSVPGMRGVVSRYAKVRYSGFDQYGEKIDRVADGFHARVVQHEYDHLIGKLYPMRITDFTR
FGFTEVLFPGLDPASDD

Sequences:

>Translated_177_residues
MIREILKMGDPRLLEVAKPVERFDTPELHEIVADMFETMHHANGAGLAAPQIGIGLQLIIFGFGNNNRYPDAPPVPETVL
INPKVEYMPPDMEEGWEGCLSVPGMRGVVSRYAKVRYSGFDQYGEKIDRVADGFHARVVQHEYDHLIGKLYPMRITDFTR
FGFTEVLFPGLDPASDD
>Mature_177_residues
MIREILKMGDPRLLEVAKPVERFDTPELHEIVADMFETMHHANGAGLAAPQIGIGLQLIIFGFGNNNRYPDAPPVPETVL
INPKVEYMPPDMEEGWEGCLSVPGMRGVVSRYAKVRYSGFDQYGEKIDRVADGFHARVVQHEYDHLIGKLYPMRITDFTR
FGFTEVLFPGLDPASDD

Specific function: Removes the formyl group from the N-terminal Met of newly synthesized proteins. Requires at least a dipeptide for an efficient rate of reaction. N-terminal L-methionine is a prerequisite for activity but the enzyme has broad specificity at other positions

COG id: COG0242

COG function: function code J; N-formylmethionyl-tRNA deformylase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the polypeptide deformylase family [H]

Homologues:

Organism=Homo sapiens, GI11641243, Length=175, Percent_Identity=32.5714285714286, Blast_Score=90, Evalue=1e-18,
Organism=Escherichia coli, GI1789682, Length=149, Percent_Identity=41.6107382550336, Blast_Score=103, Evalue=7e-24,
Organism=Drosophila melanogaster, GI24645728, Length=183, Percent_Identity=32.7868852459016, Blast_Score=99, Evalue=2e-21,
Organism=Drosophila melanogaster, GI24645726, Length=156, Percent_Identity=35.8974358974359, Blast_Score=93, Evalue=8e-20,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000181 [H]

Pfam domain/function: PF01327 Pep_deformylase [H]

EC number: =3.5.1.88 [H]

Molecular weight: Translated: 19935; Mature: 19935

Theoretical pI: Translated: 4.77; Mature: 4.77

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
4.5 %Met     (Translated Protein)
5.1 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
4.5 %Met     (Mature Protein)
5.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIREILKMGDPRLLEVAKPVERFDTPELHEIVADMFETMHHANGAGLAAPQIGIGLQLII
CHHHHHHCCCCHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHCCCCCCCCCCCCCCEEEEE
FGFGNNNRYPDAPPVPETVLINPKVEYMPPDMEEGWEGCLSVPGMRGVVSRYAKVRYSGF
EEECCCCCCCCCCCCCCEEEECCCCCCCCCCHHHHHHHHHCCCCHHHHHHHHHHHHHCCH
DQYGEKIDRVADGFHARVVQHEYDHLIGKLYPMRITDFTRFGFTEVLFPGLDPASDD
HHHHHHHHHHHCCHHHHHHHHHHHHHHHHHCCCHHHHHHHHCHHHHHCCCCCCCCCC
>Mature Secondary Structure
MIREILKMGDPRLLEVAKPVERFDTPELHEIVADMFETMHHANGAGLAAPQIGIGLQLII
CHHHHHHCCCCHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHCCCCCCCCCCCCCCEEEEE
FGFGNNNRYPDAPPVPETVLINPKVEYMPPDMEEGWEGCLSVPGMRGVVSRYAKVRYSGF
EEECCCCCCCCCCCCCCEEEECCCCCCCCCCHHHHHHHHHCCCCHHHHHHHHHHHHHCCH
DQYGEKIDRVADGFHARVVQHEYDHLIGKLYPMRITDFTRFGFTEVLFPGLDPASDD
HHHHHHHHHHHCCHHHHHHHHHHHHHHHHHCCCHHHHHHHHCHHHHHCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11823852 [H]