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The map label for this gene is ligA [H]

Identifier: 78066803

GI number: 78066803

Start: 2388447

End: 2390522

Strand: Reverse

Name: ligA [H]

Synonym: Bcep18194_A5334

Alternate gene names: 78066803

Gene position: 2390522-2388447 (Counterclockwise)

Preceding gene: 78066804

Following gene: 78066802

Centisome position: 64.71

GC content: 67.05

Gene sequence:

>2076_bases
ATGGCCCGAACCCAAGCCGAACCGCCAGCCAGCCAGCCCGACGCGCGCGCCGCGTGGCTGCGCGATCAACTCGAGCGGGC
GAACTACGCCTACTACGTGCTCGACCAGCCGGATCTGCCCGACGCCGAATACGACCGGTTGTTCCGCGAACTGCAGCAGC
TCGAAACCGACCATCCCGAACTCGTGACGCCCGATTCACCGACGCAACGCGTCGGTGGCGAGGCGGCCGGCGGCTTCACG
CCGGTCGTCCATGACGCGCCGATGCTGTCGCTGAACAACGGCTTCGCCGACGAGGACATCGCCGCATTCGACAAGCGTGT
CGCCGACGCGCTCGACAAGACGACCGATCTCGCCGGCTCGGTGACGGAGCCTGTCGAATACGCGTGCGAGCTGAAGTTCG
ACGGCCTCGCGATCTCGCTGCGCTACGAGCAGGGCGTATTCGTGCAGGCGTCGACGCGCGGTGACGGGACGACGGGCGAG
GACGTGACCGAGAACGTGCGCACGATCCGCTCGATTCCGCTGAAGCTGAAGGGTAAGCATGTGCCGGCCGTACTCGACGT
GCGCGGCGAGGTGCTGATGTTCAAGCGCGATTTCGCGCGCCTGAACGAACGCCAGCGCGCGGCCGAGCAGCGCGAATTCG
CGAACCCGCGCAACGCGGCGGCCGGTAGCTTGCGCCAGCTCGACTCGAAGATCACCGCGCAGCGTCCGCTGTCGTTCTTC
GCGTACGGGATCGGCGTGCTCGACGGGATGCCGATGCTCGATACGCACAGCGCGCTGCTCGACTGGTACGAGTCGCTCGG
CCTGCCCGTGAACCGCGAGCGCGAGGTCGTGCAAGGCGCCGAAGGCTTGCTCGGTTTCTTCCGCAAGATTGGCGAGAAGC
GCGAATCGCTGCCGTACGACATCGACGGTGTCGTCTACAAGGTCAACCGGCGTGACGAGCAGGAGCGCCTCGGCTTCGTG
TCGCGCGCGCCGCGCTTTGCGCTCGCGCACAAGTTCCCCGCGCAGGAAGCGCTGACCAAGCTCGTCGCGATCGACGTGCA
GGTCGGCCGCACGGGCGCGATCACGCCGGTCGCGCGCCTCGAGCCCGTGTTCGTCGGCGGTGCGACCGTGACAAACGCGA
CGCTGCATAACGAGGACGAAGTACGACGCAAGGACATCCGGATCGGCGATACCGTGATCGTGCGCCGCGCGGGCGACGTG
ATCCCCGAGGTGGTCGGCGCGCTGCTCGACCGGCGCCCGGCCGATGCGGCCGAATTCGTGATGCCGACCGAATGTCCGGT
GTGCGGTTCGAAGATCGAGCGCCTGCCGGACGAGGCGATCGCGCGCTGCACGGGCGGCCTGTTCTGCCCGGCACAGCGCA
AGCAGGCGCTGTGGCACTTCGCGCAGCGCCGCGCGCTCGACATCGACGGGCTCGGCGAGAAGATCATCGACCAGCTCGTC
GAGCTGAATCTCGTGCGCACGCCGGCCGACCTGTTCAATCTCGGCTTTGCGACGCTCGCCGAGCTCGACCGGTTCGCCGA
GAAGTCGGCACAGAATCTGCTCGACTCGCTCGAGAAGGCGAAGCATACGACGCTCGCGCGCTTCATCTACGGGCTGGGTA
TCCGTCATGTGGGCGAATCGACCGCGAAGGATCTCGCGAAGCATTTCGGCTCGCTGACGCCGATCATGGACGCATCGATC
GAAGAACTGCTCGAAGTCAACGACGTCGGGCCGATTGTCGCCGAGTCGCTCCATCAGTTCTTCGCGGAAGAGCACAACCG
GACCGTGATCGAGCAGTTGCGCGCACCGGGCAAGGTCACGTGGCCGGAAGGGCCGCCTGCGCCGAAGGCGCCGCAGGGCG
TGCTGGCCGGCAAGACGGTCGTGCTGACGGGCACGCTGCCGACGCTCACGCGCGATGCCGCGAAGGAAATGCTCGAAGCG
GCGGGCGCGAAGGTCGCCGGTTCGGTATCGAAGAAGACGGATTACGTGGTCGCGGGCGCCGAAGCCGGCAGCAAGCTCGT
GAAGGCCGAGGAACTCGGCATCCCCGTGCTGGACGAAGACGGTCTGCACCAACTCCTGGAGGGCAATACGCCATGA

Upstream 100 bases:

>100_bases
CGCTGCAGCACGCGCATCGGGCGCGCATTGCACGCCCCGCCGCGGCGGCAAACTGAGATAATCGGGCATCCGATTTTCTC
AGAACGACTCTGCCGCCAGC

Downstream 100 bases:

>100_bases
TTCGCGAGATCCTGAAGATGGGCGATCCGCGCCTGCTCGAAGTCGCCAAGCCGGTCGAGCGGTTCGATACCCCCGAACTG
CACGAGATCGTCGCGGACAT

Product: DNA ligase (NAD+)

Products: NA

Alternate protein names: Polydeoxyribonucleotide synthase [NAD+] [H]

Number of amino acids: Translated: 691; Mature: 690

Protein sequence:

>691_residues
MARTQAEPPASQPDARAAWLRDQLERANYAYYVLDQPDLPDAEYDRLFRELQQLETDHPELVTPDSPTQRVGGEAAGGFT
PVVHDAPMLSLNNGFADEDIAAFDKRVADALDKTTDLAGSVTEPVEYACELKFDGLAISLRYEQGVFVQASTRGDGTTGE
DVTENVRTIRSIPLKLKGKHVPAVLDVRGEVLMFKRDFARLNERQRAAEQREFANPRNAAAGSLRQLDSKITAQRPLSFF
AYGIGVLDGMPMLDTHSALLDWYESLGLPVNREREVVQGAEGLLGFFRKIGEKRESLPYDIDGVVYKVNRRDEQERLGFV
SRAPRFALAHKFPAQEALTKLVAIDVQVGRTGAITPVARLEPVFVGGATVTNATLHNEDEVRRKDIRIGDTVIVRRAGDV
IPEVVGALLDRRPADAAEFVMPTECPVCGSKIERLPDEAIARCTGGLFCPAQRKQALWHFAQRRALDIDGLGEKIIDQLV
ELNLVRTPADLFNLGFATLAELDRFAEKSAQNLLDSLEKAKHTTLARFIYGLGIRHVGESTAKDLAKHFGSLTPIMDASI
EELLEVNDVGPIVAESLHQFFAEEHNRTVIEQLRAPGKVTWPEGPPAPKAPQGVLAGKTVVLTGTLPTLTRDAAKEMLEA
AGAKVAGSVSKKTDYVVAGAEAGSKLVKAEELGIPVLDEDGLHQLLEGNTP

Sequences:

>Translated_691_residues
MARTQAEPPASQPDARAAWLRDQLERANYAYYVLDQPDLPDAEYDRLFRELQQLETDHPELVTPDSPTQRVGGEAAGGFT
PVVHDAPMLSLNNGFADEDIAAFDKRVADALDKTTDLAGSVTEPVEYACELKFDGLAISLRYEQGVFVQASTRGDGTTGE
DVTENVRTIRSIPLKLKGKHVPAVLDVRGEVLMFKRDFARLNERQRAAEQREFANPRNAAAGSLRQLDSKITAQRPLSFF
AYGIGVLDGMPMLDTHSALLDWYESLGLPVNREREVVQGAEGLLGFFRKIGEKRESLPYDIDGVVYKVNRRDEQERLGFV
SRAPRFALAHKFPAQEALTKLVAIDVQVGRTGAITPVARLEPVFVGGATVTNATLHNEDEVRRKDIRIGDTVIVRRAGDV
IPEVVGALLDRRPADAAEFVMPTECPVCGSKIERLPDEAIARCTGGLFCPAQRKQALWHFAQRRALDIDGLGEKIIDQLV
ELNLVRTPADLFNLGFATLAELDRFAEKSAQNLLDSLEKAKHTTLARFIYGLGIRHVGESTAKDLAKHFGSLTPIMDASI
EELLEVNDVGPIVAESLHQFFAEEHNRTVIEQLRAPGKVTWPEGPPAPKAPQGVLAGKTVVLTGTLPTLTRDAAKEMLEA
AGAKVAGSVSKKTDYVVAGAEAGSKLVKAEELGIPVLDEDGLHQLLEGNTP
>Mature_690_residues
ARTQAEPPASQPDARAAWLRDQLERANYAYYVLDQPDLPDAEYDRLFRELQQLETDHPELVTPDSPTQRVGGEAAGGFTP
VVHDAPMLSLNNGFADEDIAAFDKRVADALDKTTDLAGSVTEPVEYACELKFDGLAISLRYEQGVFVQASTRGDGTTGED
VTENVRTIRSIPLKLKGKHVPAVLDVRGEVLMFKRDFARLNERQRAAEQREFANPRNAAAGSLRQLDSKITAQRPLSFFA
YGIGVLDGMPMLDTHSALLDWYESLGLPVNREREVVQGAEGLLGFFRKIGEKRESLPYDIDGVVYKVNRRDEQERLGFVS
RAPRFALAHKFPAQEALTKLVAIDVQVGRTGAITPVARLEPVFVGGATVTNATLHNEDEVRRKDIRIGDTVIVRRAGDVI
PEVVGALLDRRPADAAEFVMPTECPVCGSKIERLPDEAIARCTGGLFCPAQRKQALWHFAQRRALDIDGLGEKIIDQLVE
LNLVRTPADLFNLGFATLAELDRFAEKSAQNLLDSLEKAKHTTLARFIYGLGIRHVGESTAKDLAKHFGSLTPIMDASIE
ELLEVNDVGPIVAESLHQFFAEEHNRTVIEQLRAPGKVTWPEGPPAPKAPQGVLAGKTVVLTGTLPTLTRDAAKEMLEAA
GAKVAGSVSKKTDYVVAGAEAGSKLVKAEELGIPVLDEDGLHQLLEGNTP

Specific function: DNA ligase that catalyzes the formation of phosphodiester linkages between 5'-phosphoryl and 3'-hydroxyl groups in double-stranded DNA using NAD as a coenzyme and as the energy source for the reaction. It is essential for DNA replication and repair of dam

COG id: COG0272

COG function: function code L; NAD-dependent DNA ligase (contains BRCT domain type II)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 BRCT domain [H]

Homologues:

Organism=Homo sapiens, GI32528306, Length=91, Percent_Identity=46.1538461538462, Blast_Score=74, Evalue=5e-13,
Organism=Escherichia coli, GI1788750, Length=669, Percent_Identity=58.1464872944694, Blast_Score=758, Evalue=0.0,
Organism=Saccharomyces cerevisiae, GI6324791, Length=90, Percent_Identity=44.4444444444444, Blast_Score=69, Evalue=3e-12,
Organism=Drosophila melanogaster, GI62472391, Length=92, Percent_Identity=43.4782608695652, Blast_Score=67, Evalue=6e-11,
Organism=Drosophila melanogaster, GI17737765, Length=92, Percent_Identity=43.4782608695652, Blast_Score=66, Evalue=7e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001357
- InterPro:   IPR018239
- InterPro:   IPR004150
- InterPro:   IPR001679
- InterPro:   IPR013839
- InterPro:   IPR013840
- InterPro:   IPR003583
- InterPro:   IPR012340
- InterPro:   IPR016027
- InterPro:   IPR010994
- InterPro:   IPR004149 [H]

Pfam domain/function: PF00533 BRCT; PF01653 DNA_ligase_aden; PF03120 DNA_ligase_OB; PF03119 DNA_ligase_ZBD [H]

EC number: =6.5.1.2 [H]

Molecular weight: Translated: 75561; Mature: 75429

Theoretical pI: Translated: 5.02; Mature: 5.02

Prosite motif: PS50172 BRCT ; PS01055 DNA_LIGASE_N1 ; PS01056 DNA_LIGASE_N2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
1.2 %Met     (Translated Protein)
1.9 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
1.0 %Met     (Mature Protein)
1.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MARTQAEPPASQPDARAAWLRDQLERANYAYYVLDQPDLPDAEYDRLFRELQQLETDHPE
CCCCCCCCCCCCCCHHHHHHHHHHHHCCEEEEEECCCCCCCHHHHHHHHHHHHHCCCCCC
LVTPDSPTQRVGGEAAGGFTPVVHDAPMLSLNNGFADEDIAAFDKRVADALDKTTDLAGS
EECCCCCHHHCCCCCCCCCCCHHCCCCEEECCCCCCCHHHHHHHHHHHHHHHHHHHHCCC
VTEPVEYACELKFDGLAISLRYEQGVFVQASTRGDGTTGEDVTENVRTIRSIPLKLKGKH
CCHHHHHHHEEEECCEEEEEEECCCEEEEEECCCCCCCCHHHHHHHHHHHHCCCEECCCC
VPAVLDVRGEVLMFKRDFARLNERQRAAEQREFANPRNAAAGSLRQLDSKITAQRPLSFF
CCEEEECCCCEEEHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHCCHHHH
AYGIGVLDGMPMLDTHSALLDWYESLGLPVNREREVVQGAEGLLGFFRKIGEKRESLPYD
HHHHHHHCCCCCHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCC
IDGVVYKVNRRDEQERLGFVSRAPRFALAHKFPAQEALTKLVAIDVQVGRTGAITPVARL
CCCEEEEECCCCHHHHHHHHHCCCHHHHHHCCCHHHHHHHHHHHEEEECCCCCCCHHHHC
EPVFVGGATVTNATLHNEDEVRRKDIRIGDTVIVRRAGDVIPEVVGALLDRRPADAAEFV
CEEEECCCEEECCCCCCHHHHHHHHCCCCCEEEEEHHHHHHHHHHHHHHCCCCCCHHHCC
MPTECPVCGSKIERLPDEAIARCTGGLFCPAQRKQALWHFAQRRALDIDGLGEKIIDQLV
CCCCCCCCHHHHHHCCHHHHHHHCCCCCCCHHHHHHHHHHHHHHCCCCCCCCHHHHHHHH
ELNLVRTPADLFNLGFATLAELDRFAEKSAQNLLDSLEKAKHTTLARFIYGLGIRHVGES
HHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHH
TAKDLAKHFGSLTPIMDASIEELLEVNDVGPIVAESLHQFFAEEHNRTVIEQLRAPGKVT
HHHHHHHHHCCCCHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHCCHHHHHHHCCCCCEE
WPEGPPAPKAPQGVLAGKTVVLTGTLPTLTRDAAKEMLEAAGAKVAGSVSKKTDYVVAGA
CCCCCCCCCCCCCEECCCEEEEECCCHHHHHHHHHHHHHHCCCHHHCCCCCCCCEEEECC
EAGSKLVKAEELGIPVLDEDGLHQLLEGNTP
CCCCHHHHHHHCCCCCCCCCHHHHHHCCCCC
>Mature Secondary Structure 
ARTQAEPPASQPDARAAWLRDQLERANYAYYVLDQPDLPDAEYDRLFRELQQLETDHPE
CCCCCCCCCCCCCHHHHHHHHHHHHCCEEEEEECCCCCCCHHHHHHHHHHHHHCCCCCC
LVTPDSPTQRVGGEAAGGFTPVVHDAPMLSLNNGFADEDIAAFDKRVADALDKTTDLAGS
EECCCCCHHHCCCCCCCCCCCHHCCCCEEECCCCCCCHHHHHHHHHHHHHHHHHHHHCCC
VTEPVEYACELKFDGLAISLRYEQGVFVQASTRGDGTTGEDVTENVRTIRSIPLKLKGKH
CCHHHHHHHEEEECCEEEEEEECCCEEEEEECCCCCCCCHHHHHHHHHHHHCCCEECCCC
VPAVLDVRGEVLMFKRDFARLNERQRAAEQREFANPRNAAAGSLRQLDSKITAQRPLSFF
CCEEEECCCCEEEHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHCCHHHH
AYGIGVLDGMPMLDTHSALLDWYESLGLPVNREREVVQGAEGLLGFFRKIGEKRESLPYD
HHHHHHHCCCCCHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCC
IDGVVYKVNRRDEQERLGFVSRAPRFALAHKFPAQEALTKLVAIDVQVGRTGAITPVARL
CCCEEEEECCCCHHHHHHHHHCCCHHHHHHCCCHHHHHHHHHHHEEEECCCCCCCHHHHC
EPVFVGGATVTNATLHNEDEVRRKDIRIGDTVIVRRAGDVIPEVVGALLDRRPADAAEFV
CEEEECCCEEECCCCCCHHHHHHHHCCCCCEEEEEHHHHHHHHHHHHHHCCCCCCHHHCC
MPTECPVCGSKIERLPDEAIARCTGGLFCPAQRKQALWHFAQRRALDIDGLGEKIIDQLV
CCCCCCCCHHHHHHCCHHHHHHHCCCCCCCHHHHHHHHHHHHHHCCCCCCCCHHHHHHHH
ELNLVRTPADLFNLGFATLAELDRFAEKSAQNLLDSLEKAKHTTLARFIYGLGIRHVGES
HHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHH
TAKDLAKHFGSLTPIMDASIEELLEVNDVGPIVAESLHQFFAEEHNRTVIEQLRAPGKVT
HHHHHHHHHCCCCHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHCCHHHHHHHCCCCCEE
WPEGPPAPKAPQGVLAGKTVVLTGTLPTLTRDAAKEMLEAAGAKVAGSVSKKTDYVVAGA
CCCCCCCCCCCCCEECCCEEEEECCCHHHHHHHHHHHHHHCCCHHHCCCCCCCCEEEECC
EAGSKLVKAEELGIPVLDEDGLHQLLEGNTP
CCCCHHHHHHHCCCCCCCCCHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA