Click here to switch to the map view.

The map label for this gene is psdht [H]

Identifier: 78066726

GI number: 78066726

Start: 2312524

End: 2313543

Strand: Direct

Name: psdht [H]

Synonym: Bcep18194_A5257

Alternate gene names: 78066726

Gene position: 2312524-2313543 (Clockwise)

Preceding gene: 78066725

Following gene: 78066727

Centisome position: 62.6

GC content: 68.33

Gene sequence:

>1020_bases
ATGTCGACTGAACAACAGGGCACCGCCCATCCGACGATCGACGGCGAGCCGATCCCGACGCTCGACGAAATCGCCGCGCA
GCATTTCGCGCTGTCGCCCTGGGTCGCGCGCACGCCCGTGTTCGAGCGCGCCGACCTGCCGTCCCTCGAGGGCACGCACG
TCAACTTCAAGTTCGAGTTGCTGCAGGTCAGCGGCAGCTTCAAGGCGCGCGGCGCATTCACGCACCTGCTCGCGCTCGAC
GAGGCGCGCAAGAACGCCGGCGTCACCTGCGTGTCGGCCGGCAATCACGCGGCGGCCGTCGCGTATGCGGCGATGCGGCT
CGGCAGCAGCGCCAAGGTCGTGATGTTCCATACCGCGAGCCCGTCGCGCATCGCGCAGTGCAAGCAGTACCGCGCGGAAG
TCGTGCTGGCCGGCAGCGCGTCGCAGGCGTTCGACCTGGTCCGGCGGATCGAGGCCGAGGAAGGCCGCTTCTTCATCCAT
CCGTTCAACGGGTATCACACGATCCTCGGCACCGCGACGCTCGGCTACGAATGGGCGACGCAGACGCCCGACCTCGACGC
GGTGATCGTGCCGATCGGCGGCGGCGGGCTCGCGGCCGGCATGGCGACCGCGCTGCGGCTCGCGAACCCGCGCGTGCACG
TGTACGGCGTCGAGCCGGAAGGCGCCGATGCGATGAGCCGCAGCTTCGCGGCGAATCACACGATCAAGATGAGCGCGATG
CAGACGATCGCCGATTCGCTGATGGCGCCGCATACCGAGGAATACAGCTACCAGCTGTGCCGGCGCCACGTCGACCGCAT
CGTCACCGTGTCCGACGACGCACTGCGTGCCGCGATGCTGTTCCTGTTCTCGCAACTGAAACTGTCCGTCGAACCGGCGT
GCGCGGCCGCACTGGCCGCGCTGCTCGGCCCGCTGCGCGAAACGCTGCAGGGCAAGCGCGTCGGCGTGCTGCTATCCGGC
ACCAATACCGACCCAGGCACACTCGGCATGCATCTCGCACACGCGAAATTGCAGCCCTGA

Upstream 100 bases:

>100_bases
GCGCAAGTTCATCCGCAGCATGTCGGTCGACGCGCACTGACTGCCCCCCGCCACGCCGGCCCGCGTGTGCGGGCCGGCCC
ACTCCGCCACCCGCCACGTC

Downstream 100 bases:

>100_bases
CCCCACATCGGGCCCACGCGGACAAACCTAGGGTTATCCCCAGATTATGTTGACAGCCCTGTTGATAACCCGCCGGACAA
GCACGCAAGTGGTTGAGCGC

Product: pyridoxal-5'-phosphate-dependent enzyme, beta subunit

Products: 2-oxobutanoate; NH3

Alternate protein names: NA

Number of amino acids: Translated: 339; Mature: 338

Protein sequence:

>339_residues
MSTEQQGTAHPTIDGEPIPTLDEIAAQHFALSPWVARTPVFERADLPSLEGTHVNFKFELLQVSGSFKARGAFTHLLALD
EARKNAGVTCVSAGNHAAAVAYAAMRLGSSAKVVMFHTASPSRIAQCKQYRAEVVLAGSASQAFDLVRRIEAEEGRFFIH
PFNGYHTILGTATLGYEWATQTPDLDAVIVPIGGGGLAAGMATALRLANPRVHVYGVEPEGADAMSRSFAANHTIKMSAM
QTIADSLMAPHTEEYSYQLCRRHVDRIVTVSDDALRAAMLFLFSQLKLSVEPACAAALAALLGPLRETLQGKRVGVLLSG
TNTDPGTLGMHLAHAKLQP

Sequences:

>Translated_339_residues
MSTEQQGTAHPTIDGEPIPTLDEIAAQHFALSPWVARTPVFERADLPSLEGTHVNFKFELLQVSGSFKARGAFTHLLALD
EARKNAGVTCVSAGNHAAAVAYAAMRLGSSAKVVMFHTASPSRIAQCKQYRAEVVLAGSASQAFDLVRRIEAEEGRFFIH
PFNGYHTILGTATLGYEWATQTPDLDAVIVPIGGGGLAAGMATALRLANPRVHVYGVEPEGADAMSRSFAANHTIKMSAM
QTIADSLMAPHTEEYSYQLCRRHVDRIVTVSDDALRAAMLFLFSQLKLSVEPACAAALAALLGPLRETLQGKRVGVLLSG
TNTDPGTLGMHLAHAKLQP
>Mature_338_residues
STEQQGTAHPTIDGEPIPTLDEIAAQHFALSPWVARTPVFERADLPSLEGTHVNFKFELLQVSGSFKARGAFTHLLALDE
ARKNAGVTCVSAGNHAAAVAYAAMRLGSSAKVVMFHTASPSRIAQCKQYRAEVVLAGSASQAFDLVRRIEAEEGRFFIHP
FNGYHTILGTATLGYEWATQTPDLDAVIVPIGGGGLAAGMATALRLANPRVHVYGVEPEGADAMSRSFAANHTIKMSAMQ
TIADSLMAPHTEEYSYQLCRRHVDRIVTVSDDALRAAMLFLFSQLKLSVEPACAAALAALLGPLRETLQGKRVGVLLSGT
NTDPGTLGMHLAHAKLQP

Specific function: Acts On Both Serine And Threonine, And Properly Considered As A Hydroxy Amino Acid Deaminase. [C]

COG id: COG1171

COG function: function code E; Threonine dehydratase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

Organism=Homo sapiens, GI11345492, Length=309, Percent_Identity=31.0679611650485, Blast_Score=144, Evalue=8e-35,
Organism=Homo sapiens, GI19923959, Length=300, Percent_Identity=29.6666666666667, Blast_Score=65, Evalue=1e-10,
Organism=Escherichia coli, GI1789505, Length=271, Percent_Identity=39.1143911439114, Blast_Score=147, Evalue=1e-36,
Organism=Escherichia coli, GI1790207, Length=304, Percent_Identity=31.9078947368421, Blast_Score=124, Evalue=9e-30,
Organism=Caenorhabditis elegans, GI71991565, Length=308, Percent_Identity=36.3636363636364, Blast_Score=162, Evalue=2e-40,
Organism=Caenorhabditis elegans, GI17508781, Length=318, Percent_Identity=29.559748427673, Blast_Score=128, Evalue=4e-30,
Organism=Caenorhabditis elegans, GI17537387, Length=308, Percent_Identity=32.4675324675325, Blast_Score=122, Evalue=4e-28,
Organism=Saccharomyces cerevisiae, GI6322631, Length=309, Percent_Identity=34.9514563106796, Blast_Score=158, Evalue=1e-39,
Organism=Saccharomyces cerevisiae, GI6320930, Length=274, Percent_Identity=31.021897810219, Blast_Score=102, Evalue=6e-23,
Organism=Saccharomyces cerevisiae, GI6319788, Length=284, Percent_Identity=23.943661971831, Blast_Score=82, Evalue=1e-16,
Organism=Saccharomyces cerevisiae, GI6322024, Length=168, Percent_Identity=26.1904761904762, Blast_Score=66, Evalue=1e-11,
Organism=Drosophila melanogaster, GI21355833, Length=311, Percent_Identity=35.6913183279743, Blast_Score=144, Evalue=7e-35,
Organism=Drosophila melanogaster, GI24645328, Length=211, Percent_Identity=35.5450236966825, Blast_Score=92, Evalue=3e-19,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001926 [H]

Pfam domain/function: PF00291 PALP [H]

EC number: 4.3.1.19

Molecular weight: Translated: 36227; Mature: 36096

Theoretical pI: Translated: 7.04; Mature: 7.04

Prosite motif: PS00165 DEHYDRATASE_SER_THR

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
4.1 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSTEQQGTAHPTIDGEPIPTLDEIAAQHFALSPWVARTPVFERADLPSLEGTHVNFKFEL
CCCCCCCCCCCCCCCCCCCCHHHHHHHHHCCCCCHHCCCCCCCCCCCCCCCCEEEEEEEE
LQVSGSFKARGAFTHLLALDEARKNAGVTCVSAGNHAAAVAYAAMRLGSSAKVVMFHTAS
EEECCCCCCCHHHHHHHHHHHHHHCCCEEEEECCCCHHHHHHHHHHCCCCCEEEEEECCC
PSRIAQCKQYRAEVVLAGSASQAFDLVRRIEAEEGRFFIHPFNGYHTILGTATLGYEWAT
CHHHHHHHHCCEEEEEECCCHHHHHHHHHHHHCCCEEEEEECCCEEEEEEEEEECEEECC
QTPDLDAVIVPIGGGGLAAGMATALRLANPRVHVYGVEPEGADAMSRSFAANHTIKMSAM
CCCCCCEEEEEECCCHHHHHHHHHHHHCCCEEEEEEECCCCCHHHHHHHHCCCEEEHHHH
QTIADSLMAPHTEEYSYQLCRRHVDRIVTVSDDALRAAMLFLFSQLKLSVEPACAAALAA
HHHHHHHHCCCCHHHHHHHHHHHHHHEEEECCHHHHHHHHHHHHHHHCCCCHHHHHHHHH
LLGPLRETLQGKRVGVLLSGTNTDPGTLGMHLAHAKLQP
HHHHHHHHHCCCEEEEEEECCCCCCCCCCEEEECCCCCC
>Mature Secondary Structure 
STEQQGTAHPTIDGEPIPTLDEIAAQHFALSPWVARTPVFERADLPSLEGTHVNFKFEL
CCCCCCCCCCCCCCCCCCCHHHHHHHHHCCCCCHHCCCCCCCCCCCCCCCCEEEEEEEE
LQVSGSFKARGAFTHLLALDEARKNAGVTCVSAGNHAAAVAYAAMRLGSSAKVVMFHTAS
EEECCCCCCCHHHHHHHHHHHHHHCCCEEEEECCCCHHHHHHHHHHCCCCCEEEEEECCC
PSRIAQCKQYRAEVVLAGSASQAFDLVRRIEAEEGRFFIHPFNGYHTILGTATLGYEWAT
CHHHHHHHHCCEEEEEECCCHHHHHHHHHHHHCCCEEEEEECCCEEEEEEEEEECEEECC
QTPDLDAVIVPIGGGGLAAGMATALRLANPRVHVYGVEPEGADAMSRSFAANHTIKMSAM
CCCCCCEEEEEECCCHHHHHHHHHHHHCCCEEEEEEECCCCCHHHHHHHHCCCEEEHHHH
QTIADSLMAPHTEEYSYQLCRRHVDRIVTVSDDALRAAMLFLFSQLKLSVEPACAAALAA
HHHHHHHHCCCCHHHHHHHHHHHHHHEEEECCHHHHHHHHHHHHHHHCCCCHHHHHHHHH
LLGPLRETLQGKRVGVLLSGTNTDPGTLGMHLAHAKLQP
HHHHHHHHHCCCEEEEEEECCCCCCCCCCEEEECCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: AMP; L-Val; pyridoxal 5'-phosphate [C]

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): 40 {L-Ser}} 5 {L-Ser}} 91 {L-Thr}} 70 {L-Thr}} 11 {L-Thr}} [C]

Substrates: L-threonine

Specific reaction: L-threonine = 2-oxobutanoate + NH3

General reaction: Deamination; elimination of NH alphabeta-position of amino acid [C]

Inhibitor: Ile [C]

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12596884; 8743570 [H]