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The map label for this gene is ispF

Identifier: 78066722

GI number: 78066722

Start: 2306335

End: 2306820

Strand: Reverse

Name: ispF

Synonym: Bcep18194_A5253

Alternate gene names: 78066722

Gene position: 2306820-2306335 (Counterclockwise)

Preceding gene: 78066723

Following gene: 78066717

Centisome position: 62.45

GC content: 67.9

Gene sequence:

>486_bases
ATGGATTTCAGAATCGGACAAGGCTACGACGTGCACCAGCTCGTCGAGGGGCGCCCCCTCATCATCGGCGGCGTGACGAT
TCCGTACGAACGCGGCCTGCTCGGCCACTCGGACGCGGACGTGCTGCTGCACGCGATCACCGACGCGCTGTTCGGCGCGG
CGGCGCTCGGCGACATCGGCCGCCATTTCTCCGACACGGACGCGGCATTCAAGGGCGCCGACAGCCGCGTGCTGCTGCGC
GAGTGCGTTGCACGCATCAACGCGGCCGGTTTCACGATCCAGAACGTCGACAGCACCGTGATCGCCCAGGCACCGAAGCT
GGCACCGCATATCGACGGGATGCGCGCGAACATCGCGGCCGATCTCGGGCTGCCGCTCGATCGCGTGAACGTGAAGGCGA
AGACCAACGAGAAGCTCGGCTATCTCGGCCGCGGCGAGGGGATCGAGGCGCAGGCCGCCGCGCTGCTGGTGAAGCAGGGC
GGCTGA

Upstream 100 bases:

>100_bases
TTCAAGGTCACGTACCCGGAAGATTTCGCGCTCGCGGAAGCGATCCTCGCGCGTTCCACGAACGCTTCCTGACTTTCACC
CCCTCACAATCGGAATACGC

Downstream 100 bases:

>100_bases
CGCCCGGCTGCGGCGCGCGGTTCGCCCGTCGCGCTGTGCAGCGGAAATGAAAAATGCCACGCGAATGCGTGGCATTTTCT
TTTTTTGGAGCGGCTACCGG

Product: 2-C-methyl-D-erythritol 2,4-cyclodiphosphate synthase

Products: NA

Alternate protein names: MECDP-synthase; MECPS [H]

Number of amino acids: Translated: 161; Mature: 161

Protein sequence:

>161_residues
MDFRIGQGYDVHQLVEGRPLIIGGVTIPYERGLLGHSDADVLLHAITDALFGAAALGDIGRHFSDTDAAFKGADSRVLLR
ECVARINAAGFTIQNVDSTVIAQAPKLAPHIDGMRANIAADLGLPLDRVNVKAKTNEKLGYLGRGEGIEAQAAALLVKQG
G

Sequences:

>Translated_161_residues
MDFRIGQGYDVHQLVEGRPLIIGGVTIPYERGLLGHSDADVLLHAITDALFGAAALGDIGRHFSDTDAAFKGADSRVLLR
ECVARINAAGFTIQNVDSTVIAQAPKLAPHIDGMRANIAADLGLPLDRVNVKAKTNEKLGYLGRGEGIEAQAAALLVKQG
G
>Mature_161_residues
MDFRIGQGYDVHQLVEGRPLIIGGVTIPYERGLLGHSDADVLLHAITDALFGAAALGDIGRHFSDTDAAFKGADSRVLLR
ECVARINAAGFTIQNVDSTVIAQAPKLAPHIDGMRANIAADLGLPLDRVNVKAKTNEKLGYLGRGEGIEAQAAALLVKQG
G

Specific function: Converts 4-diphosphocytidyl-2-C-methyl-D-erythritol 2- phosphate into 2-C-methyl-D-erythritol 2,4-cyclodiphosphate (MECDP) and CMP [H]

COG id: COG0245

COG function: function code I; 2C-methyl-D-erythritol 2,4-cyclodiphosphate synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ispF family [H]

Homologues:

Organism=Escherichia coli, GI1789103, Length=156, Percent_Identity=66.6666666666667, Blast_Score=192, Evalue=8e-51,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003526
- InterPro:   IPR020555 [H]

Pfam domain/function: PF02542 YgbB [H]

EC number: =4.6.1.12 [H]

Molecular weight: Translated: 16938; Mature: 16938

Theoretical pI: Translated: 6.35; Mature: 6.35

Prosite motif: PS01350 ISPF

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
1.2 %Met     (Translated Protein)
1.9 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
1.2 %Met     (Mature Protein)
1.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDFRIGQGYDVHQLVEGRPLIIGGVTIPYERGLLGHSDADVLLHAITDALFGAAALGDIG
CCCCCCCCCCHHHHHCCCCEEEECEECCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHH
RHFSDTDAAFKGADSRVLLRECVARINAAGFTIQNVDSTVIAQAPKLAPHIDGMRANIAA
CCCCCCCHHHCCCCCHHHHHHHHHHHCCCCCEEECCCCHHHHCCCCCCCCCCCCHHCEEC
DLGLPLDRVNVKAKTNEKLGYLGRGEGIEAQAAALLVKQGG
CCCCCHHHEEEEECCCCCCCCCCCCCCCCHHHEEEEEECCC
>Mature Secondary Structure
MDFRIGQGYDVHQLVEGRPLIIGGVTIPYERGLLGHSDADVLLHAITDALFGAAALGDIG
CCCCCCCCCCHHHHHCCCCEEEECEECCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHH
RHFSDTDAAFKGADSRVLLRECVARINAAGFTIQNVDSTVIAQAPKLAPHIDGMRANIAA
CCCCCCCHHHCCCCCHHHHHHHHHHHCCCCCEEECCCCHHHHCCCCCCCCCCCCHHCEEC
DLGLPLDRVNVKAKTNEKLGYLGRGEGIEAQAAALLVKQGG
CCCCCHHHEEEEECCCCCCCCCCCCCCCCHHHEEEEEECCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA