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The map label for this gene is gcl [H]

Identifier: 78066647

GI number: 78066647

Start: 2223824

End: 2225599

Strand: Direct

Name: gcl [H]

Synonym: Bcep18194_A5178

Alternate gene names: 78066647

Gene position: 2223824-2225599 (Clockwise)

Preceding gene: 78066636

Following gene: 78066648

Centisome position: 60.2

GC content: 66.1

Gene sequence:

>1776_bases
ATGGCCAAGATGAGAGCCGTCGACGCAGCTGTGCTCGTGCTCGAGAAAGAAGGCATCCAGACCGCGTTCGGCGTGCCGGG
CGCAGCGATCAACCCGTTCTACTCCGCCATGCGCAAGTCGGGTGGCATCAGCCACGTGCTGGCACGCCACGTCGAAGGCG
CGTCGCACATGGCCGAAGGCTTCACGCGTGCCGCCCCGGGCAACATCGGCGTGTGCATCGGCACGTCGGGCCCCGCCGGC
ACCGACATGATCACAGGCCTCTACTCCGCATCGGCCGACTCGATTCCGATCCTCGCGATCACGGGCCAGGCACCGCGTGC
CCGCCTGTACAAGGAAGACTTCCAGGCCGTCGACATCGAATCGATCGCGAAGCCCGTCACGAAGTGGGCCGTCACCGTGC
GTGAGCCGGCACTCGTGCCGCGCGTGTTCCAGCAGGCATTCCACCTGATGCGCTCGGGCCGTCCGGGCCCGGTGCTGGTC
GACCTGCCGATCGACGTGCAGCTCGCCGAAATCGAATTCGACATCGACACGTATGAACCGCTGCCGGTCTACAAGCCCGC
GGCCACCCGCGCGCAGATCGAGAAGGCGCTCACGATGCTCAACGACGCGGACAAGCCGCTGATCGTGTCGGGCGGCGGCG
TGCTCAACGCAGCGGCCGAAGACCTGCTCGTCCAGTTCGCCGAAACGATCGGCGTGCCGGTGATCCCGACGCTGATGTCG
TGGGGCGCGATTCCGGACGACCACCCGCTGATGGCCGGCATGGTCGGCCTGCAGACGTCGCACCGCTACGGCAACGCCAC
GATGCTCGCGTCCGACTTCGTGCTCGGCATCGGCAACCGCTGGGCGAACCGCCACACGGGCAGCGTCGAGGTCTACACGA
AGGGCCGCAAGTTCGTGCACGTCGACATCGAGCCGACGCAGATCGGCCGCGTGTTCGGCCCGGATCTCGGCATCGTGTCG
GACGCGAAGGCCGCGCTCGAGCTGTTCGTCGCCGTCGCACAGGAATGGAAGGCCGCCGGCAAGCTGAAGGACCGCAGCGC
ATGGGTCGCCGACTGCCAGGAACGCAAGCGCACGCTGCAACGCAAGACGCACTTCGACAACGTGCCGGTCAAGCCGCAGC
GCGTGTACGAAGAGATGAACAAGGTGTTCGGCCGCGATACGTGCTACGTCAGCACGATCGGCTTGTCGCAGATCGCCGCC
GCGCAGTTCCTGCACGTGTTCAAGGCGCGTAACTGGATCAACTGCGGCCAGGCCGGCCCGCTCGGCTGGACGATTCCCGC
CGCGCTCGGCGTGCGTGCAGCGGACCCGAGCCGCCCGATCGTCGCGCTGTCCGGCGACTACGACTTCCAGTTCATGATCG
AGGAACTGGCAGCCGGCGCGCAATTCAAGCTGCCGTACGTGCACGTCGTCGTGAACAACTCGTACCTCGGGCTGATCCGC
CAGGCGCAGCGTGCGTTCGACATGGACTACTGCGTGCAGCTCGCGTTCGACAACGTGAACGCACCGGAACTGAACGGCTA
TGGCGTCGACCACGTGGCCGTCGCGGAAGGCCTCGGCTGCAAGGCGCTGCGCGTGTTCAAGCCGGAAGAGATCGAGCCGG
CGCTGCGTCAGGCGCAAACGCTCGCGGAAGAGTTCAGCGTGCCGGTGGTCGTGGAAGTGATCCTCGAGCGCGTGACGAAC
ATCTCGATGGGCACCGAAATCGACGCGATCAACGAATTCGAGGATCTCGCCGAAAAGGCCGAGCACGCACCGACCGCAAT
CTCGATGCTCGACTGA

Upstream 100 bases:

>100_bases
AAAAAAGCGACTCCGGCTGATCTTATCAAACCTTTCCTGCATCCCTAAAGTGTCCTCAACAGACCCATTTGCAAGATCCA
CCCCATTCAAGGAGACATTC

Downstream 100 bases:

>100_bases
ACCGCACGACATTTTTGACTGACCGACCGAAGAGGCTTAGCTCATGCCGAAGTTTGCTGCAAACCTGACCATGCTGTTCA
ACGAAGTGCCGTTCCTCGAC

Product: glyoxylate carboligase

Products: NA

Alternate protein names: Tartronate-semialdehyde synthase [H]

Number of amino acids: Translated: 591; Mature: 590

Protein sequence:

>591_residues
MAKMRAVDAAVLVLEKEGIQTAFGVPGAAINPFYSAMRKSGGISHVLARHVEGASHMAEGFTRAAPGNIGVCIGTSGPAG
TDMITGLYSASADSIPILAITGQAPRARLYKEDFQAVDIESIAKPVTKWAVTVREPALVPRVFQQAFHLMRSGRPGPVLV
DLPIDVQLAEIEFDIDTYEPLPVYKPAATRAQIEKALTMLNDADKPLIVSGGGVLNAAAEDLLVQFAETIGVPVIPTLMS
WGAIPDDHPLMAGMVGLQTSHRYGNATMLASDFVLGIGNRWANRHTGSVEVYTKGRKFVHVDIEPTQIGRVFGPDLGIVS
DAKAALELFVAVAQEWKAAGKLKDRSAWVADCQERKRTLQRKTHFDNVPVKPQRVYEEMNKVFGRDTCYVSTIGLSQIAA
AQFLHVFKARNWINCGQAGPLGWTIPAALGVRAADPSRPIVALSGDYDFQFMIEELAAGAQFKLPYVHVVVNNSYLGLIR
QAQRAFDMDYCVQLAFDNVNAPELNGYGVDHVAVAEGLGCKALRVFKPEEIEPALRQAQTLAEEFSVPVVVEVILERVTN
ISMGTEIDAINEFEDLAEKAEHAPTAISMLD

Sequences:

>Translated_591_residues
MAKMRAVDAAVLVLEKEGIQTAFGVPGAAINPFYSAMRKSGGISHVLARHVEGASHMAEGFTRAAPGNIGVCIGTSGPAG
TDMITGLYSASADSIPILAITGQAPRARLYKEDFQAVDIESIAKPVTKWAVTVREPALVPRVFQQAFHLMRSGRPGPVLV
DLPIDVQLAEIEFDIDTYEPLPVYKPAATRAQIEKALTMLNDADKPLIVSGGGVLNAAAEDLLVQFAETIGVPVIPTLMS
WGAIPDDHPLMAGMVGLQTSHRYGNATMLASDFVLGIGNRWANRHTGSVEVYTKGRKFVHVDIEPTQIGRVFGPDLGIVS
DAKAALELFVAVAQEWKAAGKLKDRSAWVADCQERKRTLQRKTHFDNVPVKPQRVYEEMNKVFGRDTCYVSTIGLSQIAA
AQFLHVFKARNWINCGQAGPLGWTIPAALGVRAADPSRPIVALSGDYDFQFMIEELAAGAQFKLPYVHVVVNNSYLGLIR
QAQRAFDMDYCVQLAFDNVNAPELNGYGVDHVAVAEGLGCKALRVFKPEEIEPALRQAQTLAEEFSVPVVVEVILERVTN
ISMGTEIDAINEFEDLAEKAEHAPTAISMLD
>Mature_590_residues
AKMRAVDAAVLVLEKEGIQTAFGVPGAAINPFYSAMRKSGGISHVLARHVEGASHMAEGFTRAAPGNIGVCIGTSGPAGT
DMITGLYSASADSIPILAITGQAPRARLYKEDFQAVDIESIAKPVTKWAVTVREPALVPRVFQQAFHLMRSGRPGPVLVD
LPIDVQLAEIEFDIDTYEPLPVYKPAATRAQIEKALTMLNDADKPLIVSGGGVLNAAAEDLLVQFAETIGVPVIPTLMSW
GAIPDDHPLMAGMVGLQTSHRYGNATMLASDFVLGIGNRWANRHTGSVEVYTKGRKFVHVDIEPTQIGRVFGPDLGIVSD
AKAALELFVAVAQEWKAAGKLKDRSAWVADCQERKRTLQRKTHFDNVPVKPQRVYEEMNKVFGRDTCYVSTIGLSQIAAA
QFLHVFKARNWINCGQAGPLGWTIPAALGVRAADPSRPIVALSGDYDFQFMIEELAAGAQFKLPYVHVVVNNSYLGLIRQ
AQRAFDMDYCVQLAFDNVNAPELNGYGVDHVAVAEGLGCKALRVFKPEEIEPALRQAQTLAEEFSVPVVVEVILERVTNI
SMGTEIDAINEFEDLAEKAEHAPTAISMLD

Specific function: Catalyzes the condensation of two molecules of glyoxylate to give 2-hydroxy-3-oxopropanoate (also termed tartronate semialdehyde) [H]

COG id: COG3960

COG function: function code R; Glyoxylate carboligase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the TPP enzyme family [H]

Homologues:

Organism=Homo sapiens, GI93004078, Length=562, Percent_Identity=23.6654804270463, Blast_Score=131, Evalue=2e-30,
Organism=Homo sapiens, GI21361361, Length=597, Percent_Identity=24.2881072026801, Blast_Score=114, Evalue=2e-25,
Organism=Escherichia coli, GI1786717, Length=590, Percent_Identity=79.6610169491525, Blast_Score=985, Evalue=0.0,
Organism=Escherichia coli, GI87081685, Length=544, Percent_Identity=34.375, Blast_Score=310, Evalue=1e-85,
Organism=Escherichia coli, GI1790104, Length=482, Percent_Identity=33.402489626556, Blast_Score=263, Evalue=2e-71,
Organism=Escherichia coli, GI1787096, Length=553, Percent_Identity=26.2206148282098, Blast_Score=172, Evalue=8e-44,
Organism=Escherichia coli, GI1788716, Length=477, Percent_Identity=25.5765199161426, Blast_Score=135, Evalue=8e-33,
Organism=Caenorhabditis elegans, GI17542570, Length=554, Percent_Identity=25.0902527075812, Blast_Score=109, Evalue=3e-24,
Organism=Caenorhabditis elegans, GI17531299, Length=511, Percent_Identity=22.8962818003914, Blast_Score=99, Evalue=8e-21,
Organism=Caenorhabditis elegans, GI17531301, Length=511, Percent_Identity=22.8962818003914, Blast_Score=99, Evalue=9e-21,
Organism=Saccharomyces cerevisiae, GI6323755, Length=598, Percent_Identity=29.7658862876254, Blast_Score=257, Evalue=4e-69,
Organism=Saccharomyces cerevisiae, GI6320816, Length=564, Percent_Identity=24.468085106383, Blast_Score=106, Evalue=9e-24,
Organism=Drosophila melanogaster, GI19922626, Length=572, Percent_Identity=25, Blast_Score=144, Evalue=2e-34,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006397
- InterPro:   IPR012000
- InterPro:   IPR012001
- InterPro:   IPR011766 [H]

Pfam domain/function: PF02775 TPP_enzyme_C; PF00205 TPP_enzyme_M; PF02776 TPP_enzyme_N [H]

EC number: =4.1.1.47 [H]

Molecular weight: Translated: 64026; Mature: 63895

Theoretical pI: Translated: 5.50; Mature: 5.50

Prosite motif: PS01067 SECE_SEC61G

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAKMRAVDAAVLVLEKEGIQTAFGVPGAAINPFYSAMRKSGGISHVLARHVEGASHMAEG
CCCCHHHCEEEEEEECCCCCHHCCCCCCHHHHHHHHHHHCCCHHHHHHHHHCCHHHHHHH
FTRAAPGNIGVCIGTSGPAGTDMITGLYSASADSIPILAITGQAPRARLYKEDFQAVDIE
HHHCCCCCEEEEEECCCCCCHHHHHHHHHCCCCCCEEEEEECCCCHHHHHHHHCCCCCHH
SIAKPVTKWAVTVREPALVPRVFQQAFHLMRSGRPGPVLVDLPIDVQLAEIEFDIDTYEP
HHHHHHHHHEEEECCCHHHHHHHHHHHHHHHCCCCCCEEEECCCCEEEEEEEEECCCCCC
LPVYKPAATRAQIEKALTMLNDADKPLIVSGGGVLNAAAEDLLVQFAETIGVPVIPTLMS
CCCCCCCHHHHHHHHHHHHHCCCCCCEEEECCCHHHHHHHHHHHHHHHHCCCCHHHHHHH
WGAIPDDHPLMAGMVGLQTSHRYGNATMLASDFVLGIGNRWANRHTGSVEVYTKGRKFVH
CCCCCCCCCHHHHHHHHCCCCCCCCEEEEEHHHHEECCCCCCCCCCCCEEEEECCCEEEE
VDIEPTQIGRVFGPDLGIVSDAKAALELFVAVAQEWKAAGKLKDRSAWVADCQERKRTLQ
EECCHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHH
RKTHFDNVPVKPQRVYEEMNKVFGRDTCYVSTIGLSQIAAAQFLHVFKARNWINCGQAGP
HHHCCCCCCCCHHHHHHHHHHHHCCCCEEEEECCHHHHHHHHHHHHHHHCCCCCCCCCCC
LGWTIPAALGVRAADPSRPIVALSGDYDFQFMIEELAAGAQFKLPYVHVVVNNSYLGLIR
CCEEHHHHHCCCCCCCCCCEEEEECCCCHHHHHHHHCCCCEEECCEEEEEECCCHHHHHH
QAQRAFDMDYCVQLAFDNVNAPELNGYGVDHVAVAEGLGCKALRVFKPEEIEPALRQAQT
HHHHHHCHHHHHHEEECCCCCCCCCCCCCCHHHHHCCCCCCEEEECCCCCCCHHHHHHHH
LAEEFSVPVVVEVILERVTNISMGTEIDAINEFEDLAEKAEHAPTAISMLD
HHHHCCCHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHCCCCEEECCC
>Mature Secondary Structure 
AKMRAVDAAVLVLEKEGIQTAFGVPGAAINPFYSAMRKSGGISHVLARHVEGASHMAEG
CCCHHHCEEEEEEECCCCCHHCCCCCCHHHHHHHHHHHCCCHHHHHHHHHCCHHHHHHH
FTRAAPGNIGVCIGTSGPAGTDMITGLYSASADSIPILAITGQAPRARLYKEDFQAVDIE
HHHCCCCCEEEEEECCCCCCHHHHHHHHHCCCCCCEEEEEECCCCHHHHHHHHCCCCCHH
SIAKPVTKWAVTVREPALVPRVFQQAFHLMRSGRPGPVLVDLPIDVQLAEIEFDIDTYEP
HHHHHHHHHEEEECCCHHHHHHHHHHHHHHHCCCCCCEEEECCCCEEEEEEEEECCCCCC
LPVYKPAATRAQIEKALTMLNDADKPLIVSGGGVLNAAAEDLLVQFAETIGVPVIPTLMS
CCCCCCCHHHHHHHHHHHHHCCCCCCEEEECCCHHHHHHHHHHHHHHHHCCCCHHHHHHH
WGAIPDDHPLMAGMVGLQTSHRYGNATMLASDFVLGIGNRWANRHTGSVEVYTKGRKFVH
CCCCCCCCCHHHHHHHHCCCCCCCCEEEEEHHHHEECCCCCCCCCCCCEEEEECCCEEEE
VDIEPTQIGRVFGPDLGIVSDAKAALELFVAVAQEWKAAGKLKDRSAWVADCQERKRTLQ
EECCHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHH
RKTHFDNVPVKPQRVYEEMNKVFGRDTCYVSTIGLSQIAAAQFLHVFKARNWINCGQAGP
HHHCCCCCCCCHHHHHHHHHHHHCCCCEEEEECCHHHHHHHHHHHHHHHCCCCCCCCCCC
LGWTIPAALGVRAADPSRPIVALSGDYDFQFMIEELAAGAQFKLPYVHVVVNNSYLGLIR
CCEEHHHHHCCCCCCCCCCEEEEECCCCHHHHHHHHCCCCEEECCEEEEEECCCHHHHHH
QAQRAFDMDYCVQLAFDNVNAPELNGYGVDHVAVAEGLGCKALRVFKPEEIEPALRQAQT
HHHHHHCHHHHHHEEECCCCCCCCCCCCCCHHHHHCCCCCCEEEECCCCCCCHHHHHHHH
LAEEFSVPVVVEVILERVTNISMGTEIDAINEFEDLAEKAEHAPTAISMLD
HHHHCCCHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHCCCCEEECCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]