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The map label for this gene is ada [H]

Identifier: 78066401

GI number: 78066401

Start: 1949490

End: 1950554

Strand: Direct

Name: ada [H]

Synonym: Bcep18194_A4931

Alternate gene names: 78066401

Gene position: 1949490-1950554 (Clockwise)

Preceding gene: 78066400

Following gene: 78066402

Centisome position: 52.77

GC content: 62.63

Gene sequence:

>1065_bases
ATGAATACGATCACTGTCTACGAAACCGACGATGCCCGCTGGGAGGCGGTACAGGCGCGAGATGCTGCAGCCGATGGTCA
TTTCGTCTACGCCGTCAGGACCACCGGTGTCTATTGCCATCCGAGCTCGACGACACGCTTGCCCAAGCGCCAGAACGTAG
TGTTCTACGACACTGTAGAGGCTGCGGAGGCTGCGGGCTTTCGCCCCAGCCGTCGGGCGCATGGAGACCAGACCAGCGCA
GCGACGGAACGTGCGGCAATTGTGGCACGCGCCTGTCGCTTGATCGAGGCATCCGATACGCCACCCCAGCTCGACGACCT
GGCTGCCGAGATCGGCATGAGCCCGTTCCATTTCCATCGCCTCTTCAAGGCAGAGACAGGTTTGACGCCCAAGGCGTACA
GCTCGGCCTATCGTGCCCGCAAGCTTCGGGAGGAATTGAGCGCGCCGAATTCCTCGGTCACCAACGCCATCTATGGGGCT
GGCTTCAACTCGAACAGCCGGTTCTACGAAGCCTCCGACCAGCTGCTCGGGATGCGTGCGCGCGACTATAGGGCAGGCGG
TGCAGGTGCAGTCATACGTTTCGCCGTGGGGCAATGCTCACTCGGCGCCATCCTTGTGGCTCAAAGTCAGCGTGGGATCT
GCGCCATTCTGTTGGGTGACGACCCGGACATGCTGGTGCGTAATCTGCAAGACCAGTTCCCCAAGGCGCAGTTCATCGGT
GGAGAGAACGACTTCGAACAGTTGGTCGCCCGGGTGGTGGGCTTCGTCGAAGCGCCGTCGATGGGCTTGCATCTGCCGCT
GGACGTACAGGGCACAGCCTTTCAGGAGCGCGTCTGGCGCGCGCTGCGCGAGATTCCGCCGGGCACGACGATAAGTTACG
CGGAGATTGCCGAGCGAATCGGGTCGCCCAAGGCGGTGCGCGCTGTCGCGCAGGCCTGCGGGGCGAATCACCTTGCTGTG
GCCATCCCTTGCCACCGCGTTGTACGACGCGATGGAGACATCTCCGGCTATCGCTGGGGAGTAGATCGCAAGCGCGAGTT
GTTGCGCCGCGAAGCCGAGATTTGA

Upstream 100 bases:

>100_bases
GACTGCCCAGCCGATGAATTTTGATAGCAAGAATCGGAGTTCTTGCGAATGAAGTCCGGCCTATCCTGATTCCGTGATTG
ATGCTTCCAGGACTGCCACA

Downstream 100 bases:

>100_bases
AGGAGAGATTTATTGTGACTTCACCAATCACGAGTCTTGCTGGTATGGAATGGGCGGGTATTGCAGCTCAACTTGATACA
GAAGGCTATGCGGTACTCCC

Product: AraC family transcriptional regulator

Products: NA

Alternate protein names: Regulatory protein of adaptative response; O-6-methylguanine-DNA alkyltransferase [H]

Number of amino acids: Translated: 354; Mature: 354

Protein sequence:

>354_residues
MNTITVYETDDARWEAVQARDAAADGHFVYAVRTTGVYCHPSSTTRLPKRQNVVFYDTVEAAEAAGFRPSRRAHGDQTSA
ATERAAIVARACRLIEASDTPPQLDDLAAEIGMSPFHFHRLFKAETGLTPKAYSSAYRARKLREELSAPNSSVTNAIYGA
GFNSNSRFYEASDQLLGMRARDYRAGGAGAVIRFAVGQCSLGAILVAQSQRGICAILLGDDPDMLVRNLQDQFPKAQFIG
GENDFEQLVARVVGFVEAPSMGLHLPLDVQGTAFQERVWRALREIPPGTTISYAEIAERIGSPKAVRAVAQACGANHLAV
AIPCHRVVRRDGDISGYRWGVDRKRELLRREAEI

Sequences:

>Translated_354_residues
MNTITVYETDDARWEAVQARDAAADGHFVYAVRTTGVYCHPSSTTRLPKRQNVVFYDTVEAAEAAGFRPSRRAHGDQTSA
ATERAAIVARACRLIEASDTPPQLDDLAAEIGMSPFHFHRLFKAETGLTPKAYSSAYRARKLREELSAPNSSVTNAIYGA
GFNSNSRFYEASDQLLGMRARDYRAGGAGAVIRFAVGQCSLGAILVAQSQRGICAILLGDDPDMLVRNLQDQFPKAQFIG
GENDFEQLVARVVGFVEAPSMGLHLPLDVQGTAFQERVWRALREIPPGTTISYAEIAERIGSPKAVRAVAQACGANHLAV
AIPCHRVVRRDGDISGYRWGVDRKRELLRREAEI
>Mature_354_residues
MNTITVYETDDARWEAVQARDAAADGHFVYAVRTTGVYCHPSSTTRLPKRQNVVFYDTVEAAEAAGFRPSRRAHGDQTSA
ATERAAIVARACRLIEASDTPPQLDDLAAEIGMSPFHFHRLFKAETGLTPKAYSSAYRARKLREELSAPNSSVTNAIYGA
GFNSNSRFYEASDQLLGMRARDYRAGGAGAVIRFAVGQCSLGAILVAQSQRGICAILLGDDPDMLVRNLQDQFPKAQFIG
GENDFEQLVARVVGFVEAPSMGLHLPLDVQGTAFQERVWRALREIPPGTTISYAEIAERIGSPKAVRAVAQACGANHLAV
AIPCHRVVRRDGDISGYRWGVDRKRELLRREAEI

Specific function: The methylated ADA protein acts as a positive regulator of its own synthesis, as well as that of other proteins. The transcription-activating function of the ADA protein resides in its N-terminus. It activates the transcription of alkA, alkB and aidB [H]

COG id: COG0350

COG function: function code L; Methylated DNA-protein cysteine methyltransferase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH araC/xylS-type DNA-binding domain [H]

Homologues:

Organism=Homo sapiens, GI197304670, Length=124, Percent_Identity=33.0645161290323, Blast_Score=66, Evalue=5e-11,
Organism=Escherichia coli, GI1788542, Length=345, Percent_Identity=53.9130434782609, Blast_Score=375, Evalue=1e-105,
Organism=Escherichia coli, GI1787596, Length=90, Percent_Identity=46.6666666666667, Blast_Score=93, Evalue=2e-20,
Organism=Caenorhabditis elegans, GI115533070, Length=124, Percent_Identity=41.1290322580645, Blast_Score=101, Evalue=8e-22,
Organism=Caenorhabditis elegans, GI115533068, Length=131, Percent_Identity=41.9847328244275, Blast_Score=100, Evalue=9e-22,
Organism=Saccharomyces cerevisiae, GI6320001, Length=83, Percent_Identity=44.578313253012, Blast_Score=94, Evalue=3e-20,
Organism=Drosophila melanogaster, GI17137554, Length=123, Percent_Identity=42.2764227642276, Blast_Score=79, Evalue=7e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004026
- InterPro:   IPR016221
- InterPro:   IPR009057
- InterPro:   IPR012287
- InterPro:   IPR018062
- InterPro:   IPR018060
- InterPro:   IPR001497
- InterPro:   IPR014048
- InterPro:   IPR008332
- InterPro:   IPR011991 [H]

Pfam domain/function: PF02805 Ada_Zn_binding; PF01035 DNA_binding_1; PF00165 HTH_AraC; PF02870 Methyltransf_1N [H]

EC number: =2.1.1.63 [H]

Molecular weight: Translated: 38754; Mature: 38754

Theoretical pI: Translated: 8.13; Mature: 8.13

Prosite motif: PS00041 HTH_ARAC_FAMILY_1 ; PS01124 HTH_ARAC_FAMILY_2 ; PS00374 MGMT

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.7 %Cys     (Translated Protein)
1.4 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
1.7 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNTITVYETDDARWEAVQARDAAADGHFVYAVRTTGVYCHPSSTTRLPKRQNVVFYDTVE
CCEEEEEECCCCHHHHHHHHCCCCCCCEEEEEEECEEEECCCCCCCCCCCCCEEEEECHH
AAEAAGFRPSRRAHGDQTSAATERAAIVARACRLIEASDTPPQLDDLAAEIGMSPFHFHR
HHHHCCCCCCHHCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHCCCHHHHHH
LFKAETGLTPKAYSSAYRARKLREELSAPNSSVTNAIYGAGFNSNSRFYEASDQLLGMRA
HHHHHCCCCHHHHHHHHHHHHHHHHHCCCCCHHHHHHEECCCCCCCCEEECHHHHHCCHH
RDYRAGGAGAVIRFAVGQCSLGAILVAQSQRGICAILLGDDPDMLVRNLQDQFPKAQFIG
HHCCCCCCCEEEEEECCCCCCCEEEEEECCCCEEEEEECCCHHHHHHHHHHHCCCEEECC
GENDFEQLVARVVGFVEAPSMGLHLPLDVQGTAFQERVWRALREIPPGTTISYAEIAERI
CCCHHHHHHHHHHHHHCCCCCCCEEEECCCCCHHHHHHHHHHHHCCCCCEECHHHHHHHH
GSPKAVRAVAQACGANHLAVAIPCHRVVRRDGDISGYRWGVDRKRELLRREAEI
CCCHHHHHHHHHCCCCCEEEEECHHHHHHCCCCCCCEEECCHHHHHHHHHHCCC
>Mature Secondary Structure
MNTITVYETDDARWEAVQARDAAADGHFVYAVRTTGVYCHPSSTTRLPKRQNVVFYDTVE
CCEEEEEECCCCHHHHHHHHCCCCCCCEEEEEEECEEEECCCCCCCCCCCCCEEEEECHH
AAEAAGFRPSRRAHGDQTSAATERAAIVARACRLIEASDTPPQLDDLAAEIGMSPFHFHR
HHHHCCCCCCHHCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHCCCHHHHHH
LFKAETGLTPKAYSSAYRARKLREELSAPNSSVTNAIYGAGFNSNSRFYEASDQLLGMRA
HHHHHCCCCHHHHHHHHHHHHHHHHHCCCCCHHHHHHEECCCCCCCCEEECHHHHHCCHH
RDYRAGGAGAVIRFAVGQCSLGAILVAQSQRGICAILLGDDPDMLVRNLQDQFPKAQFIG
HHCCCCCCCEEEEEECCCCCCCEEEEEECCCCEEEEEECCCHHHHHHHHHHHCCCEEECC
GENDFEQLVARVVGFVEAPSMGLHLPLDVQGTAFQERVWRALREIPPGTTISYAEIAERI
CCCHHHHHHHHHHHHHCCCCCCCEEEECCCCCHHHHHHHHHHHHCCCCCEECHHHHHHHH
GSPKAVRAVAQACGANHLAVAIPCHRVVRRDGDISGYRWGVDRKRELLRREAEI
CCCHHHHHHHHHCCCCCEEEEECHHHHHHCCCCCCCEEECCHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 2987251; 3887409; 9097040; 9278503; 2982792; 3536913; 3009022; 3529081; 1581309; 8202360; 8500619; 8156986 [H]