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The map label for this gene is ogt [H]

Identifier: 78066399

GI number: 78066399

Start: 1948208

End: 1948759

Strand: Direct

Name: ogt [H]

Synonym: Bcep18194_A4929

Alternate gene names: 78066399

Gene position: 1948208-1948759 (Clockwise)

Preceding gene: 78066398

Following gene: 78066400

Centisome position: 52.74

GC content: 63.77

Gene sequence:

>552_bases
ATGCACATGTATAAGAGTGTCGTGGCATCGCCACTGGGTGACATGCTTCTCGTCACGGACGCGCAGCGAGCTGTGCGTGC
GCTCGATTTCGCCGACCACCAGGCACGCCTCGATCGTGGCCTGCGCGAGCACTATGGCCGTGTTGGCTTGACTGAGGTCA
CCGCGCCTGACGAGATCGCCGACGCCATCACGCGTTACTTCTCGGGTGAGCTTGCGGCGTTGGACACGCTCCGGACGGAA
ACTGCCGGGTCCGACCTGCAGCGCCGCGTGTGGGCTGCGTTGCGCCGTATCCCGACGGGCACGACCACTACCTACGGCAA
ACTCGCGAAGGCGCTGGGATTCGACGATCCACGCGCGGCAATCGACATTGGCGCTGCCAATGGCGCCAACCCGATCGCGA
TCATCGTGCCGTGCCATCGCGTCATCGCCGCCAATGGCGATCTCAAGGGCTACGCATGGGGCCTACATCGTAAGCGCTGG
TTGCTGGAGCATGAAGGGGCAATCCCGCCGCAACGCACCGAACCCCAAACTGCCACGCTGCCAGGATTTTGA

Upstream 100 bases:

>100_bases
GGCGAGACCTATCTGTTTGGGGAATGGGGTGTCACCCGGCTGAAATGAACCCAATCGGAGCGCCCAATCTGGTCTTCCGA
CTCACCTCGCAAGGAACATC

Downstream 100 bases:

>100_bases
TTTTGACCGACACCATCTCAGCCGACCTGTCTCCGCGCGCCTATCGGAAAGCTGCTCAGTTCCTGTCGGACTTCGACAAG
GACTGGGCTAGTCATATCGC

Product: methylated-DNA--(protein)-cysteine S-methyltransferase

Products: NA

Alternate protein names: 6-O-methylguanine-DNA methyltransferase; MGMT; O-6-methylguanine-DNA-alkyltransferase [H]

Number of amino acids: Translated: 183; Mature: 183

Protein sequence:

>183_residues
MHMYKSVVASPLGDMLLVTDAQRAVRALDFADHQARLDRGLREHYGRVGLTEVTAPDEIADAITRYFSGELAALDTLRTE
TAGSDLQRRVWAALRRIPTGTTTTYGKLAKALGFDDPRAAIDIGAANGANPIAIIVPCHRVIAANGDLKGYAWGLHRKRW
LLEHEGAIPPQRTEPQTATLPGF

Sequences:

>Translated_183_residues
MHMYKSVVASPLGDMLLVTDAQRAVRALDFADHQARLDRGLREHYGRVGLTEVTAPDEIADAITRYFSGELAALDTLRTE
TAGSDLQRRVWAALRRIPTGTTTTYGKLAKALGFDDPRAAIDIGAANGANPIAIIVPCHRVIAANGDLKGYAWGLHRKRW
LLEHEGAIPPQRTEPQTATLPGF
>Mature_183_residues
MHMYKSVVASPLGDMLLVTDAQRAVRALDFADHQARLDRGLREHYGRVGLTEVTAPDEIADAITRYFSGELAALDTLRTE
TAGSDLQRRVWAALRRIPTGTTTTYGKLAKALGFDDPRAAIDIGAANGANPIAIIVPCHRVIAANGDLKGYAWGLHRKRW
LLEHEGAIPPQRTEPQTATLPGF

Specific function: Involved in the cellular defense against the biological effects of O6-methylguanine (O6-MeG) in DNA. Repairs alkylated guanine in DNA by stoichiometrically transferring the alkyl group at the O-6 position to a cysteine residue in the enzyme. This is a sui

COG id: COG0350

COG function: function code L; Methylated DNA-protein cysteine methyltransferase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the MGMT family [H]

Homologues:

Organism=Homo sapiens, GI197304670, Length=77, Percent_Identity=40.2597402597403, Blast_Score=67, Evalue=6e-12,
Organism=Escherichia coli, GI1787596, Length=170, Percent_Identity=43.5294117647059, Blast_Score=151, Evalue=3e-38,
Organism=Escherichia coli, GI1788542, Length=157, Percent_Identity=34.3949044585987, Blast_Score=89, Evalue=3e-19,
Organism=Caenorhabditis elegans, GI115533068, Length=107, Percent_Identity=38.3177570093458, Blast_Score=80, Evalue=6e-16,
Organism=Caenorhabditis elegans, GI115533070, Length=107, Percent_Identity=38.3177570093458, Blast_Score=79, Evalue=1e-15,
Organism=Saccharomyces cerevisiae, GI6320001, Length=83, Percent_Identity=43.3734939759036, Blast_Score=80, Evalue=1e-16,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001497
- InterPro:   IPR014048
- InterPro:   IPR008332
- InterPro:   IPR011991 [H]

Pfam domain/function: PF01035 DNA_binding_1; PF02870 Methyltransf_1N [H]

EC number: =2.1.1.63 [H]

Molecular weight: Translated: 19951; Mature: 19951

Theoretical pI: Translated: 8.48; Mature: 8.48

Prosite motif: PS00374 MGMT

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
2.2 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
2.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MHMYKSVVASPLGDMLLVTDAQRAVRALDFADHQARLDRGLREHYGRVGLTEVTAPDEIA
CCHHHHHHHCCCCCEEEEECHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHH
DAITRYFSGELAALDTLRTETAGSDLQRRVWAALRRIPTGTTTTYGKLAKALGFDDPRAA
HHHHHHHCCCHHHHHHHHHHHCCHHHHHHHHHHHHHCCCCCCHHHHHHHHHHCCCCCCCE
IDIGAANGANPIAIIVPCHRVIAANGDLKGYAWGLHRKRWLLEHEGAIPPQRTEPQTATL
EEECCCCCCCCEEEEEECHHHEECCCCCCCEEEHHHHHHHHHHCCCCCCCCCCCCCCCCC
PGF
CCC
>Mature Secondary Structure
MHMYKSVVASPLGDMLLVTDAQRAVRALDFADHQARLDRGLREHYGRVGLTEVTAPDEIA
CCHHHHHHHCCCCCEEEEECHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHH
DAITRYFSGELAALDTLRTETAGSDLQRRVWAALRRIPTGTTTTYGKLAKALGFDDPRAA
HHHHHHHCCCHHHHHHHHHHHCCHHHHHHHHHHHHHCCCCCCHHHHHHHHHHCCCCCCCE
IDIGAANGANPIAIIVPCHRVIAANGDLKGYAWGLHRKRWLLEHEGAIPPQRTEPQTATL
EEECCCCCCCCEEEEEECHHHEECCCCCCCEEEHHHHHHHHHHCCCCCCCCCCCCCCCCC
PGF
CCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11677608; 12644504 [H]