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The map label for this gene is emrA [H]

Identifier: 78066359

GI number: 78066359

Start: 1904469

End: 1905848

Strand: Direct

Name: emrA [H]

Synonym: Bcep18194_A4889

Alternate gene names: 78066359

Gene position: 1904469-1905848 (Clockwise)

Preceding gene: 78066358

Following gene: 78066360

Centisome position: 51.55

GC content: 71.45

Gene sequence:

>1380_bases
ATGAGCAGCGACAACTCCCTGCAGACTCCCGCGCGACCGGCCGCCGCCGATCCTGCCAGCGCCCGCCAGCAACCGGCGGC
CGATCACACCGCGGCGCCGCGCGCGCCAGGCGGGCCGGGCGGGCCTGATGCCCGCACGCAACCGGCACCGGGCGGCGGCG
CACCCGCGACGCCGCCGCCACCGCCCGCTCGGCGCAAGCATCTGGTGCGCGCGCTCGTCGCCGTGTTCGCGCTCGGCATC
ATCGGCTGGGGCATCTGGTACGTGATCGTCGGGCGCTGGTACGAATCCACCGACAATGCGTACGCGCAAGGCAACGTCGT
CGAGCTGACGCCGCAAGTGACGGGCACCGTCGTGTCGATCGACGCGGACGACGGCAACCTCGTACGCGCCGGCACGCCGC
TCGTCAGTCTCGACCCGAGCGACGCGCAGATCGCGCTCGCAACCGCGCAGGCCGATCTCGCGGCAACGGTGCGCAAGGTG
CGCGGCCTGTACAGCAACGAACGCGGGCTGGCGAACAGCATCGACGGCGCACGCGCCGAACTCGATACGCGCCGCACCGC
GCTCGACAAGGCGAAGGCCGACTACAGCCGCCGCGAGGATCTCGGCCGCTCCGGCGCGATTTCGCGCGAGGAACTCGCGC
ATGCACGCGACACGCTGACGTCCGCGCAAAGCGCTTACGCGGCCGCGCAGAGCGCGCTGGCAACGTTGAGCGAGCAGCAC
CAGACGAGCAAGGCGCTGATCGACGACACCGCTGTCGCGTCACACCCGGACGTGCAGGCCGCGGCCGAACGACTGCGGGC
CGCCTATCTCGCGCATGCCCGCACCCAGGTTGTCACGCCCGTCACCGGCTATGTCGCGAAGCGTACGGTGCAGGTCGGGC
AGCGCGTGTCGCCGGGCGTGCCGCTGCTTGCGGTCGTGCCGCTGCGCGAGCTGTGGGTCGACGCGAATTTCACCGAGAAG
CAGCTCGAGCACATGCGCATCGGGCAGCCGGTCGAACTGACCGCCGACCTGTACGGTGACGCGGTGCGCTACACCGGCCG
CGTGCAGAGCCTGGGCGTCGGCACCGGCAGCGCGTTCTCGCTGCTGCCCGCGCAGAACGCGACCGGCAACTGGATCAAGA
TCGTGCAGCGGCTGCCGGTCCGCATCGAACTGACCGACCCGGCGCAGCTCGACGCGCATCCGCTGCGCATCGGCCTGTCG
ATGCACGCGCGCGTCGACCAGCACGACCGCAGCGGGGCGATGCTGTCGCATCGCGAGCAGCGCGCGCCGATATTCCACAC
CGACGTGTACCGCGACGAGCTCGCGCATGCCGACAGCCTGATCGCCGACGTCATCCATGCGAACCTGCCGGCGGCGGTGC
GTGCGGAGGCCGTGCGATGA

Upstream 100 bases:

>100_bases
GTCGCTGCAGCTCGTCGCCGCGCTCGGTGGCGGCTTCCGCGCCGAGCCGGACGACGCCGCGCTTGCCGCCACGCCTTCCC
AACGTCCTTGACATCGAACC

Downstream 100 bases:

>100_bases
GCGGCCCGATGGCGGCCGCCGACAGCGGCTTTCGTCCGTCGAGCGTCGGGCTGACGACCTTCGGCCTCGCGCTCGCGGTC
TTCATGCAGGTGCTGGACGG

Product: HlyD family secretion protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 459; Mature: 458

Protein sequence:

>459_residues
MSSDNSLQTPARPAAADPASARQQPAADHTAAPRAPGGPGGPDARTQPAPGGGAPATPPPPPARRKHLVRALVAVFALGI
IGWGIWYVIVGRWYESTDNAYAQGNVVELTPQVTGTVVSIDADDGNLVRAGTPLVSLDPSDAQIALATAQADLAATVRKV
RGLYSNERGLANSIDGARAELDTRRTALDKAKADYSRREDLGRSGAISREELAHARDTLTSAQSAYAAAQSALATLSEQH
QTSKALIDDTAVASHPDVQAAAERLRAAYLAHARTQVVTPVTGYVAKRTVQVGQRVSPGVPLLAVVPLRELWVDANFTEK
QLEHMRIGQPVELTADLYGDAVRYTGRVQSLGVGTGSAFSLLPAQNATGNWIKIVQRLPVRIELTDPAQLDAHPLRIGLS
MHARVDQHDRSGAMLSHREQRAPIFHTDVYRDELAHADSLIADVIHANLPAAVRAEAVR

Sequences:

>Translated_459_residues
MSSDNSLQTPARPAAADPASARQQPAADHTAAPRAPGGPGGPDARTQPAPGGGAPATPPPPPARRKHLVRALVAVFALGI
IGWGIWYVIVGRWYESTDNAYAQGNVVELTPQVTGTVVSIDADDGNLVRAGTPLVSLDPSDAQIALATAQADLAATVRKV
RGLYSNERGLANSIDGARAELDTRRTALDKAKADYSRREDLGRSGAISREELAHARDTLTSAQSAYAAAQSALATLSEQH
QTSKALIDDTAVASHPDVQAAAERLRAAYLAHARTQVVTPVTGYVAKRTVQVGQRVSPGVPLLAVVPLRELWVDANFTEK
QLEHMRIGQPVELTADLYGDAVRYTGRVQSLGVGTGSAFSLLPAQNATGNWIKIVQRLPVRIELTDPAQLDAHPLRIGLS
MHARVDQHDRSGAMLSHREQRAPIFHTDVYRDELAHADSLIADVIHANLPAAVRAEAVR
>Mature_458_residues
SSDNSLQTPARPAAADPASARQQPAADHTAAPRAPGGPGGPDARTQPAPGGGAPATPPPPPARRKHLVRALVAVFALGII
GWGIWYVIVGRWYESTDNAYAQGNVVELTPQVTGTVVSIDADDGNLVRAGTPLVSLDPSDAQIALATAQADLAATVRKVR
GLYSNERGLANSIDGARAELDTRRTALDKAKADYSRREDLGRSGAISREELAHARDTLTSAQSAYAAAQSALATLSEQHQ
TSKALIDDTAVASHPDVQAAAERLRAAYLAHARTQVVTPVTGYVAKRTVQVGQRVSPGVPLLAVVPLRELWVDANFTEKQ
LEHMRIGQPVELTADLYGDAVRYTGRVQSLGVGTGSAFSLLPAQNATGNWIKIVQRLPVRIELTDPAQLDAHPLRIGLSM
HARVDQHDRSGAMLSHREQRAPIFHTDVYRDELAHADSLIADVIHANLPAAVRAEAVR

Specific function: The emr locus confers resistance to substances of high hydrophobicity. EmrA probably participate in a transport system to extrude toxins and drugs from the cell [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cell inner membrane; Single-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the membrane fusion protein (MFP) (TC 8.A.1) family [H]

Homologues:

Organism=Escherichia coli, GI1789041, Length=394, Percent_Identity=44.9238578680203, Blast_Score=337, Evalue=8e-94,
Organism=Escherichia coli, GI1788711, Length=347, Percent_Identity=44.956772334294, Blast_Score=286, Evalue=2e-78,
Organism=Escherichia coli, GI1790519, Length=319, Percent_Identity=29.153605015674, Blast_Score=106, Evalue=4e-24,
Organism=Escherichia coli, GI87081951, Length=325, Percent_Identity=28.6153846153846, Blast_Score=101, Evalue=1e-22,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005694
- InterPro:   IPR006143 [H]

Pfam domain/function: PF00529 HlyD [H]

EC number: NA

Molecular weight: Translated: 48796; Mature: 48665

Theoretical pI: Translated: 7.57; Mature: 7.57

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
0.9 %Met     (Translated Protein)
0.9 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
0.7 %Met     (Mature Protein)
0.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSSDNSLQTPARPAAADPASARQQPAADHTAAPRAPGGPGGPDARTQPAPGGGAPATPPP
CCCCCCCCCCCCCCCCCCHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCC
PPARRKHLVRALVAVFALGIIGWGIWYVIVGRWYESTDNAYAQGNVVELTPQVTGTVVSI
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEECCCEEEECCCCCEEEEEE
DADDGNLVRAGTPLVSLDPSDAQIALATAQADLAATVRKVRGLYSNERGLANSIDGARAE
ECCCCCEEECCCCEEEECCCCCEEEEEEHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHH
LDTRRTALDKAKADYSRREDLGRSGAISREELAHARDTLTSAQSAYAAAQSALATLSEQH
HHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
QTSKALIDDTAVASHPDVQAAAERLRAAYLAHARTQVVTPVTGYVAKRTVQVGQRVSPGV
HHHHHHHHCHHHCCCCCHHHHHHHHHHHHHHHHHHHEECCHHHHHHHHHHHHCCCCCCCC
PLLAVVPLRELWVDANFTEKQLEHMRIGQPVELTADLYGDAVRYTGRVQSLGVGTGSAFS
CEEEECCHHHHHCCCCCCHHHHHHHHCCCCCEEHHHHHCCHHHHCCCHHEECCCCCCCEE
LLPAQNATGNWIKIVQRLPVRIELTDPAQLDAHPLRIGLSMHARVDQHDRSGAMLSHREQ
EECCCCCCCHHHHHHHHCCEEEEECCCCCCCCCEEEEEEEECCCCCCCCCCCCHHHHHHH
RAPIFHTDVYRDELAHADSLIADVIHANLPAAVRAEAVR
CCCEEEHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHCCC
>Mature Secondary Structure 
SSDNSLQTPARPAAADPASARQQPAADHTAAPRAPGGPGGPDARTQPAPGGGAPATPPP
CCCCCCCCCCCCCCCCCHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCC
PPARRKHLVRALVAVFALGIIGWGIWYVIVGRWYESTDNAYAQGNVVELTPQVTGTVVSI
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEECCCEEEECCCCCEEEEEE
DADDGNLVRAGTPLVSLDPSDAQIALATAQADLAATVRKVRGLYSNERGLANSIDGARAE
ECCCCCEEECCCCEEEECCCCCEEEEEEHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHH
LDTRRTALDKAKADYSRREDLGRSGAISREELAHARDTLTSAQSAYAAAQSALATLSEQH
HHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
QTSKALIDDTAVASHPDVQAAAERLRAAYLAHARTQVVTPVTGYVAKRTVQVGQRVSPGV
HHHHHHHHCHHHCCCCCHHHHHHHHHHHHHHHHHHHEECCHHHHHHHHHHHHCCCCCCCC
PLLAVVPLRELWVDANFTEKQLEHMRIGQPVELTADLYGDAVRYTGRVQSLGVGTGSAFS
CEEEECCHHHHHCCCCCCHHHHHHHHCCCCCEEHHHHHCCHHHHCCCHHEECCCCCCCEE
LLPAQNATGNWIKIVQRLPVRIELTDPAQLDAHPLRIGLSMHARVDQHDRSGAMLSHREQ
EECCCCCCCHHHHHHHHCCEEEEECCCCCCCCCEEEEEEEECCCCCCCCCCCCHHHHHHH
RAPIFHTDVYRDELAHADSLIADVIHANLPAAVRAEAVR
CCCEEEHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 1409590; 9205837; 9278503; 8203018 [H]