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The map label for this gene is emrB [H]

Identifier: 78066360

GI number: 78066360

Start: 1905845

End: 1907383

Strand: Direct

Name: emrB [H]

Synonym: Bcep18194_A4890

Alternate gene names: 78066360

Gene position: 1905845-1907383 (Clockwise)

Preceding gene: 78066359

Following gene: 78066362

Centisome position: 51.59

GC content: 67.38

Gene sequence:

>1539_bases
ATGAGCGGCCCGATGGCGGCCGCCGACAGCGGCTTTCGTCCGTCGAGCGTCGGGCTGACGACCTTCGGCCTCGCGCTCGC
GGTCTTCATGCAGGTGCTGGACGGCACCGTCGCGAACGTGTCGCTGCCGACGATCGCCGGCAACTTCGGCGTGAGCACGA
CGCAGAGCGCGTGGGTCGTCACGACGTTCTCGGTCAGCAATGCGATCGCGCTGCCGCTGACCGGGTTTCTCGTCAAGCGC
GTCGGACAGGTGCGGCTGTTCGTGTGGGCGACGCTTGCGTTCACGTTCGCATCGCTGCTGTGCGGCTTCGCGCAGAACCT
GCCGCAGCTCATTGCGTTCCGCGCGCTGCAGGGCCTGGTGGCCGGGCCGATGATCCCGACCACGCAAGCGCTGATGCTGT
CGATCTACCCGCCGCAGCGGCGCGGCTTCGCGCTGTCGATGATCGCGATGGTCACCGTCGTCGCGCCGATCACGGGGCCC
GTGTTCGGCGGCTGGGTCACCGAGCACTACTCGTGGCGCTGGGCGTTCCTGATTAACCTGCCGATCGGCGTGTTCGCCGC
GATGTGCGTGTTCGCGCAGATGCGTGCGCGCGTCGAGACGACGGTTGCCGCGCGTGTCGACTATATCGGCCTCGCCGCGT
TGATCGTCGGCGTCGGCGCGCTGCAGATCGTGCTCGACAAGGGCAATGAAGCCGACTGGTTCAACTCGACGTTCATCGTC
GTGATGTCGGTGGTCGCCGCGTTCGGCATCGCGCTGTTCCTGATCTGGGAGCTCAACGAGCCGAATCCGATCGTGAACCT
GCGGCTCTTCGCGCATCGCAACTTCGCGGTCGGCACGCTGACGCTCGTGCTCGCGTATTCGGCGTTCTTCGCGGTCAACG
TGATCGTGCCGCAATGGCTGCAGCGCACGCTCGGCTACACGGCATTCTGGGCAGGGCTCGCCGTCGCGCCGATGGGCGTG
ATCCCGGTGCTGATGACGCCGTTCATGGGCAAGTACGCGCCGCGCTTCAACATGCGGATGCTCGTGTGCTGCGCGTTCGC
GATCCTCGGCACGTCGTCGTTCCTGCGCGCGGGCTTCGTGCCCGACATCGACTTCACGCACATCGCACTGATCCAGCTGC
TGCAGGGCCTCGGCCTCGCGCTGTTCATCATGCCGATCAACAGCATCCTGCTGTCCGACCTGAAGCCGGACGAGATCGCC
GCCGGCTCCGGCCTGTCGACGTTCCTGCGCACGCTCGGCGCGAGCTTCGCGGTGTCGATCACGTCGTTCCTGTGGGACCG
GCGCGCGATCCTGCATCACGCGCAGCTCAGCGAGCACGTGAGCGGGTTCGATCCGGCCGTGCGGGCCGAAGTCGCGCGGC
TTGGCGGTGGCGATCCGCAACGCGCGGCCACGCTGCTGAGCAACCTGGTCGACGCACAGGCGTACCAGATCTCATTCAAC
GACGTTTCGTTCGCGCTCGGCTGCGTGTATCTCGCGGTGATCGCGGCGGTCTGGCTCGCCCGTCCGCCGTTCGGCCCCGG
TGCCGCCGCGAAGCGGTGA

Upstream 100 bases:

>100_bases
ACACCGACGTGTACCGCGACGAGCTCGCGCATGCCGACAGCCTGATCGCCGACGTCATCCATGCGAACCTGCCGGCGGCG
GTGCGTGCGGAGGCCGTGCG

Downstream 100 bases:

>100_bases
CGAAGCTGCGCGGCCTCGGACGACGCGCCGGTGATGCCGTCAAGCAGACGAGGGCACGGGCCGGGGCGGTGCCGGCAGTC
GTGGTAGTTGCCGGGTAGGG

Product: EmrB/QacA family drug resistance transporter

Products: Proton [Cytoplasm]; multidrug [Periplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 512; Mature: 511

Protein sequence:

>512_residues
MSGPMAAADSGFRPSSVGLTTFGLALAVFMQVLDGTVANVSLPTIAGNFGVSTTQSAWVVTTFSVSNAIALPLTGFLVKR
VGQVRLFVWATLAFTFASLLCGFAQNLPQLIAFRALQGLVAGPMIPTTQALMLSIYPPQRRGFALSMIAMVTVVAPITGP
VFGGWVTEHYSWRWAFLINLPIGVFAAMCVFAQMRARVETTVAARVDYIGLAALIVGVGALQIVLDKGNEADWFNSTFIV
VMSVVAAFGIALFLIWELNEPNPIVNLRLFAHRNFAVGTLTLVLAYSAFFAVNVIVPQWLQRTLGYTAFWAGLAVAPMGV
IPVLMTPFMGKYAPRFNMRMLVCCAFAILGTSSFLRAGFVPDIDFTHIALIQLLQGLGLALFIMPINSILLSDLKPDEIA
AGSGLSTFLRTLGASFAVSITSFLWDRRAILHHAQLSEHVSGFDPAVRAEVARLGGGDPQRAATLLSNLVDAQAYQISFN
DVSFALGCVYLAVIAAVWLARPPFGPGAAAKR

Sequences:

>Translated_512_residues
MSGPMAAADSGFRPSSVGLTTFGLALAVFMQVLDGTVANVSLPTIAGNFGVSTTQSAWVVTTFSVSNAIALPLTGFLVKR
VGQVRLFVWATLAFTFASLLCGFAQNLPQLIAFRALQGLVAGPMIPTTQALMLSIYPPQRRGFALSMIAMVTVVAPITGP
VFGGWVTEHYSWRWAFLINLPIGVFAAMCVFAQMRARVETTVAARVDYIGLAALIVGVGALQIVLDKGNEADWFNSTFIV
VMSVVAAFGIALFLIWELNEPNPIVNLRLFAHRNFAVGTLTLVLAYSAFFAVNVIVPQWLQRTLGYTAFWAGLAVAPMGV
IPVLMTPFMGKYAPRFNMRMLVCCAFAILGTSSFLRAGFVPDIDFTHIALIQLLQGLGLALFIMPINSILLSDLKPDEIA
AGSGLSTFLRTLGASFAVSITSFLWDRRAILHHAQLSEHVSGFDPAVRAEVARLGGGDPQRAATLLSNLVDAQAYQISFN
DVSFALGCVYLAVIAAVWLARPPFGPGAAAKR
>Mature_511_residues
SGPMAAADSGFRPSSVGLTTFGLALAVFMQVLDGTVANVSLPTIAGNFGVSTTQSAWVVTTFSVSNAIALPLTGFLVKRV
GQVRLFVWATLAFTFASLLCGFAQNLPQLIAFRALQGLVAGPMIPTTQALMLSIYPPQRRGFALSMIAMVTVVAPITGPV
FGGWVTEHYSWRWAFLINLPIGVFAAMCVFAQMRARVETTVAARVDYIGLAALIVGVGALQIVLDKGNEADWFNSTFIVV
MSVVAAFGIALFLIWELNEPNPIVNLRLFAHRNFAVGTLTLVLAYSAFFAVNVIVPQWLQRTLGYTAFWAGLAVAPMGVI
PVLMTPFMGKYAPRFNMRMLVCCAFAILGTSSFLRAGFVPDIDFTHIALIQLLQGLGLALFIMPINSILLSDLKPDEIAA
GSGLSTFLRTLGASFAVSITSFLWDRRAILHHAQLSEHVSGFDPAVRAEVARLGGGDPQRAATLLSNLVDAQAYQISFND
VSFALGCVYLAVIAAVWLARPPFGPGAAAKR

Specific function: Translocase that confers resistance to substances of high hydrophobicity [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the major facilitator superfamily. EmrB family [H]

Homologues:

Organism=Escherichia coli, GI1789042, Length=489, Percent_Identity=46.0122699386503, Blast_Score=456, Evalue=1e-129,
Organism=Escherichia coli, GI1788710, Length=487, Percent_Identity=40.6570841889117, Blast_Score=404, Evalue=1e-114,
Organism=Escherichia coli, GI1790195, Length=401, Percent_Identity=28.6783042394015, Blast_Score=160, Evalue=2e-40,
Organism=Escherichia coli, GI1788392, Length=403, Percent_Identity=28.287841191067, Blast_Score=128, Evalue=9e-31,
Organism=Escherichia coli, GI87081983, Length=426, Percent_Identity=24.8826291079812, Blast_Score=119, Evalue=6e-28,
Organism=Escherichia coli, GI1788509, Length=136, Percent_Identity=30.8823529411765, Blast_Score=65, Evalue=1e-11,
Organism=Escherichia coli, GI87082320, Length=211, Percent_Identity=25.5924170616114, Blast_Score=64, Evalue=3e-11,
Organism=Saccharomyces cerevisiae, GI6322958, Length=204, Percent_Identity=27.9411764705882, Blast_Score=80, Evalue=8e-16,
Organism=Saccharomyces cerevisiae, GI6325455, Length=130, Percent_Identity=33.0769230769231, Blast_Score=79, Evalue=2e-15,
Organism=Saccharomyces cerevisiae, GI6319770, Length=305, Percent_Identity=24.9180327868852, Blast_Score=67, Evalue=8e-12,
Organism=Saccharomyces cerevisiae, GI6321663, Length=150, Percent_Identity=25.3333333333333, Blast_Score=65, Evalue=2e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004638
- InterPro:   IPR020846
- InterPro:   IPR011701
- InterPro:   IPR016196 [H]

Pfam domain/function: PF07690 MFS_1 [H]

EC number: NA

Molecular weight: Translated: 54835; Mature: 54704

Theoretical pI: Translated: 9.44; Mature: 9.44

Prosite motif: PS50850 MFS

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
4.1 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
3.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSGPMAAADSGFRPSSVGLTTFGLALAVFMQVLDGTVANVSLPTIAGNFGVSTTQSAWVV
CCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHCCCCCEEECCCEECCCCCCCCCCEEEE
TTFSVSNAIALPLTGFLVKRVGQVRLFVWATLAFTFASLLCGFAQNLPQLIAFRALQGLV
EEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
AGPMIPTTQALMLSIYPPQRRGFALSMIAMVTVVAPITGPVFGGWVTEHYSWRWAFLINL
CCCCCCCCHHHEEEECCCCCCCHHHHHHHHHHHHHHHCCCHHCCHHHHCCCEEEEEEEEC
PIGVFAAMCVFAQMRARVETTVAARVDYIGLAALIVGVGALQIVLDKGNEADWFNSTFIV
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEEEECCCCCCCCCHHHHH
VMSVVAAFGIALFLIWELNEPNPIVNLRLFAHRNFAVGTLTLVLAYSAFFAVNVIVPQWL
HHHHHHHHHHHHHHEEECCCCCCEEEEEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHH
QRTLGYTAFWAGLAVAPMGVIPVLMTPFMGKYAPRFNMRMLVCCAFAILGTSSFLRAGFV
HHHHCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHCCHHHHHCCCC
PDIDFTHIALIQLLQGLGLALFIMPINSILLSDLKPDEIAAGSGLSTFLRTLGASFAVSI
CCCCHHHHHHHHHHHHCCHHHHHHHHHHHHHCCCCCCHHCCCCCHHHHHHHHCCHHHHHH
TSFLWDRRAILHHAQLSEHVSGFDPAVRAEVARLGGGDPQRAATLLSNLVDAQAYQISFN
HHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHCCCCCHHHHHHHHHHHHHCCEEEEEEC
DVSFALGCVYLAVIAAVWLARPPFGPGAAAKR
HHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCC
>Mature Secondary Structure 
SGPMAAADSGFRPSSVGLTTFGLALAVFMQVLDGTVANVSLPTIAGNFGVSTTQSAWVV
CCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHCCCCCEEECCCEECCCCCCCCCCEEEE
TTFSVSNAIALPLTGFLVKRVGQVRLFVWATLAFTFASLLCGFAQNLPQLIAFRALQGLV
EEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
AGPMIPTTQALMLSIYPPQRRGFALSMIAMVTVVAPITGPVFGGWVTEHYSWRWAFLINL
CCCCCCCCHHHEEEECCCCCCCHHHHHHHHHHHHHHHCCCHHCCHHHHCCCEEEEEEEEC
PIGVFAAMCVFAQMRARVETTVAARVDYIGLAALIVGVGALQIVLDKGNEADWFNSTFIV
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEEEECCCCCCCCCHHHHH
VMSVVAAFGIALFLIWELNEPNPIVNLRLFAHRNFAVGTLTLVLAYSAFFAVNVIVPQWL
HHHHHHHHHHHHHHEEECCCCCCEEEEEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHHH
QRTLGYTAFWAGLAVAPMGVIPVLMTPFMGKYAPRFNMRMLVCCAFAILGTSSFLRAGFV
HHHHCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHCCHHHHHCCCC
PDIDFTHIALIQLLQGLGLALFIMPINSILLSDLKPDEIAAGSGLSTFLRTLGASFAVSI
CCCCHHHHHHHHHHHHCCHHHHHHHHHHHHHCCCCCCHHCCCCCHHHHHHHHCCHHHHHH
TSFLWDRRAILHHAQLSEHVSGFDPAVRAEVARLGGGDPQRAATLLSNLVDAQAYQISFN
HHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHCCCCCHHHHHHHHHHHHHCCEEEEEEC
DVSFALGCVYLAVIAAVWLARPPFGPGAAAKR
HHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Proton [Periplasm]; multidrug [Cytoplasm] [C]

Specific reaction: Proton [Periplasm] + multidrug [Cytoplasm] = Proton [Cytoplasm] + multidrug [Periplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]