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The map label for this gene is chiA [H]

Identifier: 78066288

GI number: 78066288

Start: 1833493

End: 1834848

Strand: Reverse

Name: chiA [H]

Synonym: Bcep18194_A4818

Alternate gene names: 78066288

Gene position: 1834848-1833493 (Counterclockwise)

Preceding gene: 78066289

Following gene: 78066283

Centisome position: 49.67

GC content: 67.63

Gene sequence:

>1356_bases
ATGAACAACAATGTGTTGCCGCGTTTCATCCCGCGCGGACTTGCAGCGGGTTGCCTTCTTGCAGTGGCGAGCGTGTCCCA
GGGCGCCGGCGTCTATGCGCCTTATGTCGACGTGACGCTTTATCCGACGCCGCTCGTCGATCAGATCGGCGTCCGGCAAG
GCATCCAGCAGTTCACGCTCGCGTTCGTGGTCGCGGGCAACGGTTGTGTGCCGTCGTGGGGCGGCGTGCAGCCGATCGGC
AACGGCGCGAGCGGCGGCCTGCTGACCGCCCTGTCAACGTCGATCGCGAGCTATCGCGCGAAAGGCGGCGAAGTCGCCGT
GTCGTTCGGCGGCGCGAACGGCACGCCGCTGATGCAGGCGTGCACGACGGTCCCCGCGCTGGCGAGCGCGTACCAGACCG
TGATCGACACGTACGGCCTCACGCACCTCGACTTCGACATCGAGGGTGTGTCGCAGCTGGATACGGCCGCGGTCGCGCGC
AACTTCCAGGCGGTCACGCAGTTGCAGGCGAGCTATGCGGCGAAAGGCAAGCCGCTGCACGTCACGCTGACGCTGCCGAC
GATGCCGACCGGGCTCACGCAGGACGGCCTGAACGTCGTCAACGCGGCGATCTCGAACCAGACCGTGTTCGATGCGGTGA
ACGTGATGGCGATGGATTACGGCCCCGCGAACCTCGACATGGGCGCGGCCGCGATCAGTGCCGCGCAGGCGCTGTATTCG
CAGCTCGACACGGCGTTCAAGTCGGCCGGCCAGCCGAAGACCAATGCTCAGCTCTGGCAGATGGTCGGCGTCACGCCGAT
GATCGGCGTGAACGATGTGCAAGGCGAAACCTTCACGCTCGCGAATGCGCAAAGCGTGCTGAACGCGGCGGTCGGCAACG
GCTACGGCTTCTTCGGCAACTGGTCGGTCGGCCGCGATCAGGCGTGCACGTCCGGCGGCACGTCTGCGTCGCCGACCTGT
TCGGGCGTCGCGCAGCAGCCGTACGCGTTCGCGACGATCTTCAAGCAGCTCGACGGCAAGTGGGGCGCGGGCGTCACGCA
GGATCCGAACTACGGCGGCGGCTCGGACGGCGGCACGCCGCAGCCGGGCGCGCCCTGGGCAGCCGGCCAGGTCTATACGG
CCGGTATGACGGTCACCTACCAGGGCACGACGTATCAGGCGCAATGGTGGACGCAGGGCGACGTGCCCGGCCAGGCCGCC
GTGTGGAAGCCGGTCGGCGGCGGCACGGCCACGTGGTCGGCGACCACCGCGTATTCAGGCGGCACCTGCGTGATGTACCA
GGGCGCGAAATACTGCGCGAAATGGTGGACGCAAGGCGACAACCCGAGTGCCGGTGGGGTGTGGGTGAAATCGTGA

Upstream 100 bases:

>100_bases
GAATCCTCCAAGATGGGCACGCCGTCGGACGGGCATGCCCGGTCGCCCGACCGGCGACGATACGCGGGCCAGCGTATCCC
TCTTCCACTTGGAGAACACA

Downstream 100 bases:

>100_bases
TGCGGCATTGCAGCCGCCGCTAGGCCTCAGGCCTGCGCATCAAGGACCGTTCACGGCGATCGCCAGCTACCGGATCGCCG
GCGGACGGCCCGCTGTTCGG

Product: glycosyl hydrolase family chitinase

Products: NA

Alternate protein names: Chitinase; Lysozyme [H]

Number of amino acids: Translated: 451; Mature: 451

Protein sequence:

>451_residues
MNNNVLPRFIPRGLAAGCLLAVASVSQGAGVYAPYVDVTLYPTPLVDQIGVRQGIQQFTLAFVVAGNGCVPSWGGVQPIG
NGASGGLLTALSTSIASYRAKGGEVAVSFGGANGTPLMQACTTVPALASAYQTVIDTYGLTHLDFDIEGVSQLDTAAVAR
NFQAVTQLQASYAAKGKPLHVTLTLPTMPTGLTQDGLNVVNAAISNQTVFDAVNVMAMDYGPANLDMGAAAISAAQALYS
QLDTAFKSAGQPKTNAQLWQMVGVTPMIGVNDVQGETFTLANAQSVLNAAVGNGYGFFGNWSVGRDQACTSGGTSASPTC
SGVAQQPYAFATIFKQLDGKWGAGVTQDPNYGGGSDGGTPQPGAPWAAGQVYTAGMTVTYQGTTYQAQWWTQGDVPGQAA
VWKPVGGGTATWSATTAYSGGTCVMYQGAKYCAKWWTQGDNPSAGGVWVKS

Sequences:

>Translated_451_residues
MNNNVLPRFIPRGLAAGCLLAVASVSQGAGVYAPYVDVTLYPTPLVDQIGVRQGIQQFTLAFVVAGNGCVPSWGGVQPIG
NGASGGLLTALSTSIASYRAKGGEVAVSFGGANGTPLMQACTTVPALASAYQTVIDTYGLTHLDFDIEGVSQLDTAAVAR
NFQAVTQLQASYAAKGKPLHVTLTLPTMPTGLTQDGLNVVNAAISNQTVFDAVNVMAMDYGPANLDMGAAAISAAQALYS
QLDTAFKSAGQPKTNAQLWQMVGVTPMIGVNDVQGETFTLANAQSVLNAAVGNGYGFFGNWSVGRDQACTSGGTSASPTC
SGVAQQPYAFATIFKQLDGKWGAGVTQDPNYGGGSDGGTPQPGAPWAAGQVYTAGMTVTYQGTTYQAQWWTQGDVPGQAA
VWKPVGGGTATWSATTAYSGGTCVMYQGAKYCAKWWTQGDNPSAGGVWVKS
>Mature_451_residues
MNNNVLPRFIPRGLAAGCLLAVASVSQGAGVYAPYVDVTLYPTPLVDQIGVRQGIQQFTLAFVVAGNGCVPSWGGVQPIG
NGASGGLLTALSTSIASYRAKGGEVAVSFGGANGTPLMQACTTVPALASAYQTVIDTYGLTHLDFDIEGVSQLDTAAVAR
NFQAVTQLQASYAAKGKPLHVTLTLPTMPTGLTQDGLNVVNAAISNQTVFDAVNVMAMDYGPANLDMGAAAISAAQALYS
QLDTAFKSAGQPKTNAQLWQMVGVTPMIGVNDVQGETFTLANAQSVLNAAVGNGYGFFGNWSVGRDQACTSGGTSASPTC
SGVAQQPYAFATIFKQLDGKWGAGVTQDPNYGGGSDGGTPQPGAPWAAGQVYTAGMTVTYQGTTYQAQWWTQGDVPGQAA
VWKPVGGGTATWSATTAYSGGTCVMYQGAKYCAKWWTQGDNPSAGGVWVKS

Specific function: Bifunctional enzyme with lysozyme/chitinase activity [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Periplasm [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 5 chitin-binding type-3 domains [H]

Homologues:

Organism=Escherichia coli, GI1789736, Length=318, Percent_Identity=39.622641509434, Blast_Score=206, Evalue=3e-54,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003610
- InterPro:   IPR017853
- InterPro:   IPR013781 [H]

Pfam domain/function: PF02839 CBM_5_12 [H]

EC number: =3.2.1.14; =3.2.1.17 [H]

Molecular weight: Translated: 46289; Mature: 46289

Theoretical pI: Translated: 4.91; Mature: 4.91

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNNNVLPRFIPRGLAAGCLLAVASVSQGAGVYAPYVDVTLYPTPLVDQIGVRQGIQQFTL
CCCCCCHHHHHHHHHHHHHHHHHHHCCCCCEEECEEEEEECCCCCHHHHHHHHHHHHEEE
AFVVAGNGCVPSWGGVQPIGNGASGGLLTALSTSIASYRAKGGEVAVSFGGANGTPLMQA
EEEEECCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHCCCCEEEEEECCCCCCHHHHH
CTTVPALASAYQTVIDTYGLTHLDFDIEGVSQLDTAAVARNFQAVTQLQASYAAKGKPLH
HHHHHHHHHHHHHHHHHCCCEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEE
VTLTLPTMPTGLTQDGLNVVNAAISNQTVFDAVNVMAMDYGPANLDMGAAAISAAQALYS
EEEECCCCCCCCCCCCHHHHHHHHCCCHHHHHHEEEEECCCCCCCCHHHHHHHHHHHHHH
QLDTAFKSAGQPKTNAQLWQMVGVTPMIGVNDVQGETFTLANAQSVLNAAVGNGYGFFGN
HHHHHHHHCCCCCCCHHHHHHHCCCCCCCCCCCCCCEEEECCHHHHHHHHHCCCCCCCCC
WSVGRDQACTSGGTSASPTCSGVAQQPYAFATIFKQLDGKWGAGVTQDPNYGGGSDGGTP
CCCCCCCHHCCCCCCCCCCCCCCCCCCHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCC
QPGAPWAAGQVYTAGMTVTYQGTTYQAQWWTQGDVPGQAAVWKPVGGGTATWSATTAYSG
CCCCCCCCCEEEECCEEEEECCCEEEEEEEECCCCCCCCEEEECCCCCCEECEEEEECCC
GTCVMYQGAKYCAKWWTQGDNPSAGGVWVKS
CEEEEECCHHHHHHHHCCCCCCCCCCEEEEC
>Mature Secondary Structure
MNNNVLPRFIPRGLAAGCLLAVASVSQGAGVYAPYVDVTLYPTPLVDQIGVRQGIQQFTL
CCCCCCHHHHHHHHHHHHHHHHHHHCCCCCEEECEEEEEECCCCCHHHHHHHHHHHHEEE
AFVVAGNGCVPSWGGVQPIGNGASGGLLTALSTSIASYRAKGGEVAVSFGGANGTPLMQA
EEEEECCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHCCCCEEEEEECCCCCCHHHHH
CTTVPALASAYQTVIDTYGLTHLDFDIEGVSQLDTAAVARNFQAVTQLQASYAAKGKPLH
HHHHHHHHHHHHHHHHHCCCEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEE
VTLTLPTMPTGLTQDGLNVVNAAISNQTVFDAVNVMAMDYGPANLDMGAAAISAAQALYS
EEEECCCCCCCCCCCCHHHHHHHHCCCHHHHHHEEEEECCCCCCCCHHHHHHHHHHHHHH
QLDTAFKSAGQPKTNAQLWQMVGVTPMIGVNDVQGETFTLANAQSVLNAAVGNGYGFFGN
HHHHHHHHCCCCCCCHHHHHHHCCCCCCCCCCCCCCEEEECCHHHHHHHHHCCCCCCCCC
WSVGRDQACTSGGTSASPTCSGVAQQPYAFATIFKQLDGKWGAGVTQDPNYGGGSDGGTP
CCCCCCCHHCCCCCCCCCCCCCCCCCCHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCC
QPGAPWAAGQVYTAGMTVTYQGTTYQAQWWTQGDVPGQAAVWKPVGGGTATWSATTAYSG
CCCCCCCCCEEEECCEEEEECCCEEEEEEEECCCCCCCCEEEECCCCCCEECEEEEECCC
GTCVMYQGAKYCAKWWTQGDNPSAGGVWVKS
CEEEEECCHHHHHHHHCCCCCCCCCCEEEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9278503; 2661540; 10419961; 10760150 [H]