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The map label for this gene is ynfL [H]

Identifier: 78066289

GI number: 78066289

Start: 1835111

End: 1836013

Strand: Reverse

Name: ynfL [H]

Synonym: Bcep18194_A4819

Alternate gene names: 78066289

Gene position: 1836013-1835111 (Counterclockwise)

Preceding gene: 78066291

Following gene: 78066288

Centisome position: 49.7

GC content: 70.54

Gene sequence:

>903_bases
ATGCTCGACCTGCGCCGCCTCCGCTATTTCGTGGTCGTCGCCGACGAACTGCACTTCGGCCGCGCGGCGTTGCGCCTGCG
GGTCGCGCAGCCGCCGCTGACGCGCCATATCGCCGCACTCGAAAGCGAGCTCGGCGTCCGGTTGTTCGAACGTTCGACAC
GTGCGGTGCGGCTGACGCCGGAAGGCGCCGCGTTCCTCGACCATGCTCGCAACGTCGTCAGCGCGGCCGCCGAGGCAGAA
GCGAGCGCACGCAAGATTGCGCAAGGCATCGCCGGGCGGATCGTGATCGGCTATGCGAGCTCGATTCCGATGTCCGACAC
GTTCCCGGACGTCATCCGCGTCGCGAGCCGCACGATGCCCGACGTCGAACTCGCGTTCCGCGAGGCGGCGACCGCCAGCC
AGCGCCAGCAGATCGCGGACGGCACGATGGACATCGGTTTCGGCTGGGCGTGGAACGGTGCGCCTGGCGTGCCGGTGGCG
TCGCTCGTCGTGTCGCGCGAACCGCTCGTCGCGGCGGTGCCGGCCGGCGATCCGCTCGCGAGCCGTGCGAGCGTCGACAT
CGCGGAGCTTGCGCACGCGACCTTCGTCACCTTTCCGCCCGGTTACGGTTCGGCACTGACCGCTGCACTGGACGACCTGT
GCGCGCAAGCCGGATTCGTGCCGCGGATCGGCGCGACCGCCGCGCAGATCACGACGCTCGTGTCGCTGGTGGCCGCCGAA
CGCGGCATCGCGATCGTGCCGGGTTTCACGGCGACGCTGCAGCGGCCGGGCGTCGTCTACGTGCCGCTCGCCGGCACGCG
CGCGGTCGAGCAGATCGTGTCGTGGAACGAGCCGTTCGCGTCGGTGTGCGTCGAGCGCTTCGTCGCACTGGCACGCTCGC
TGGCGCAGCCTCCATCTGCTTGA

Upstream 100 bases:

>100_bases
GTTCATCGGAGCTGCCTGGTCGTCGATGCCGACAGTCTGACGAGTGCGTCGTGTGCACGCCAATACCGTTTTCATAGCGA
TTGATACCGGAACGACAACA

Downstream 100 bases:

>100_bases
AGCGCAACGAACGACGCGCGTCGGGCCGCCCTCACTCACTCACTCGATCGATCGGGAAAATCACCCGCGTCACGCGTTCC
GGAACTGTGTCGAATCGGCA

Product: LysR family transcriptional regulator

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 300; Mature: 300

Protein sequence:

>300_residues
MLDLRRLRYFVVVADELHFGRAALRLRVAQPPLTRHIAALESELGVRLFERSTRAVRLTPEGAAFLDHARNVVSAAAEAE
ASARKIAQGIAGRIVIGYASSIPMSDTFPDVIRVASRTMPDVELAFREAATASQRQQIADGTMDIGFGWAWNGAPGVPVA
SLVVSREPLVAAVPAGDPLASRASVDIAELAHATFVTFPPGYGSALTAALDDLCAQAGFVPRIGATAAQITTLVSLVAAE
RGIAIVPGFTATLQRPGVVYVPLAGTRAVEQIVSWNEPFASVCVERFVALARSLAQPPSA

Sequences:

>Translated_300_residues
MLDLRRLRYFVVVADELHFGRAALRLRVAQPPLTRHIAALESELGVRLFERSTRAVRLTPEGAAFLDHARNVVSAAAEAE
ASARKIAQGIAGRIVIGYASSIPMSDTFPDVIRVASRTMPDVELAFREAATASQRQQIADGTMDIGFGWAWNGAPGVPVA
SLVVSREPLVAAVPAGDPLASRASVDIAELAHATFVTFPPGYGSALTAALDDLCAQAGFVPRIGATAAQITTLVSLVAAE
RGIAIVPGFTATLQRPGVVYVPLAGTRAVEQIVSWNEPFASVCVERFVALARSLAQPPSA
>Mature_300_residues
MLDLRRLRYFVVVADELHFGRAALRLRVAQPPLTRHIAALESELGVRLFERSTRAVRLTPEGAAFLDHARNVVSAAAEAE
ASARKIAQGIAGRIVIGYASSIPMSDTFPDVIRVASRTMPDVELAFREAATASQRQQIADGTMDIGFGWAWNGAPGVPVA
SLVVSREPLVAAVPAGDPLASRASVDIAELAHATFVTFPPGYGSALTAALDDLCAQAGFVPRIGATAAQITTLVSLVAAE
RGIAIVPGFTATLQRPGVVYVPLAGTRAVEQIVSWNEPFASVCVERFVALARSLAQPPSA

Specific function: Unknown

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH lysR-type DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1787879, Length=271, Percent_Identity=38.3763837638376, Blast_Score=180, Evalue=8e-47,
Organism=Escherichia coli, GI2367136, Length=262, Percent_Identity=34.7328244274809, Blast_Score=145, Evalue=3e-36,
Organism=Escherichia coli, GI1788887, Length=293, Percent_Identity=30.3754266211604, Blast_Score=130, Evalue=1e-31,
Organism=Escherichia coli, GI1790399, Length=267, Percent_Identity=29.9625468164794, Blast_Score=106, Evalue=1e-24,
Organism=Escherichia coli, GI1788297, Length=246, Percent_Identity=24.7967479674797, Blast_Score=87, Evalue=1e-18,
Organism=Escherichia coli, GI157672245, Length=260, Percent_Identity=26.1538461538462, Blast_Score=81, Evalue=8e-17,
Organism=Escherichia coli, GI145693105, Length=273, Percent_Identity=26.007326007326, Blast_Score=80, Evalue=1e-16,
Organism=Escherichia coli, GI1788748, Length=252, Percent_Identity=26.5873015873016, Blast_Score=73, Evalue=2e-14,
Organism=Escherichia coli, GI1787601, Length=269, Percent_Identity=25.6505576208178, Blast_Score=72, Evalue=5e-14,
Organism=Escherichia coli, GI1787530, Length=262, Percent_Identity=24.0458015267176, Blast_Score=65, Evalue=7e-12,
Organism=Escherichia coli, GI145693193, Length=109, Percent_Identity=33.0275229357798, Blast_Score=64, Evalue=2e-11,

Paralogues:

None

Copy number: 10-20 Molecules/Cell [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000847
- InterPro:   IPR005119
- InterPro:   IPR011991 [H]

Pfam domain/function: PF00126 HTH_1; PF03466 LysR_substrate [H]

EC number: NA

Molecular weight: Translated: 31630; Mature: 31630

Theoretical pI: Translated: 7.58; Mature: 7.58

Prosite motif: PS50931 HTH_LYSR

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
1.3 %Met     (Translated Protein)
2.0 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
1.3 %Met     (Mature Protein)
2.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLDLRRLRYFVVVADELHFGRAALRLRVAQPPLTRHIAALESELGVRLFERSTRAVRLTP
CCCHHHEEEEEEEECCHHHCHHHEEEEECCCCHHHHHHHHHHHHHHHHHHCCCCEEEECC
EGAAFLDHARNVVSAAAEAEASARKIAQGIAGRIVIGYASSIPMSDTFPDVIRVASRTMP
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCEEEEEECCCCCCCCCHHHHHHHHHCCCC
DVELAFREAATASQRQQIADGTMDIGFGWAWNGAPGVPVASLVVSREPLVAAVPAGDPLA
HHHHHHHHHHHHHHHHHHHCCCEECCCEEECCCCCCCCHHHHHHCCCCCEEEECCCCCCC
SRASVDIAELAHATFVTFPPGYGSALTAALDDLCAQAGFVPRIGATAAQITTLVSLVAAE
HHCCCHHHHHHHHEEEECCCCCCHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHC
RGIAIVPGFTATLQRPGVVYVPLAGTRAVEQIVSWNEPFASVCVERFVALARSLAQPPSA
CCEEEECCCHHHHCCCCEEEEECCCHHHHHHHHCCCCCHHHHHHHHHHHHHHHHCCCCCC
>Mature Secondary Structure
MLDLRRLRYFVVVADELHFGRAALRLRVAQPPLTRHIAALESELGVRLFERSTRAVRLTP
CCCHHHEEEEEEEECCHHHCHHHEEEEECCCCHHHHHHHHHHHHHHHHHHCCCCEEEECC
EGAAFLDHARNVVSAAAEAEASARKIAQGIAGRIVIGYASSIPMSDTFPDVIRVASRTMP
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCEEEEEECCCCCCCCCHHHHHHHHHCCCC
DVELAFREAATASQRQQIADGTMDIGFGWAWNGAPGVPVASLVVSREPLVAAVPAGDPLA
HHHHHHHHHHHHHHHHHHHCCCEECCCEEECCCCCCCCHHHHHHCCCCCEEEECCCCCCC
SRASVDIAELAHATFVTFPPGYGSALTAALDDLCAQAGFVPRIGATAAQITTLVSLVAAE
HHCCCHHHHHHHHEEEECCCCCCHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHC
RGIAIVPGFTATLQRPGVVYVPLAGTRAVEQIVSWNEPFASVCVERFVALARSLAQPPSA
CCEEEECCCHHHHCCCCEEEEECCCHHHHHHHHCCCCCHHHHHHHHHHHHHHHHCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9097039; 9278503 [H]