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The map label for this gene is oprM [H]

Identifier: 78066239

GI number: 78066239

Start: 1768402

End: 1769859

Strand: Direct

Name: oprM [H]

Synonym: Bcep18194_A4769

Alternate gene names: 78066239

Gene position: 1768402-1769859 (Clockwise)

Preceding gene: 78066238

Following gene: 78066241

Centisome position: 47.87

GC content: 71.95

Gene sequence:

>1458_bases
ATGTTTGCGCTGAATGCACGCGCTGCGCTGCGGGTCCAGGTTGCGCTCGCCGCCGCGCTCGCGCTGGCCGGCTGCACGCT
CGCGCCGCGTTACGAGCGGCCGGCGGCGCCGGTGCCGGCGACCTATGCGCCGGTCGACGGCAGTGCCGCACCGGCGGCCG
AGGTGTCGCCGGCTAACGCTGCGCTGCTCGACGACTGGCGCACCTACTTTACCGACGCCGCGCTGCAGGCGTGGATCGAC
GCGGCGCTCGCGAACAACCGCGACCTGCGGGTCGCGGCCGGGCGCCTCGAGGAAGCGCGCGCGCTGTACGGCGTGCAGCG
CGCGGACCTGATGCCGTCGGTCGATGCGAATCTGGGCTATGACCGTGCGCGCCAGTACGACCCGGTCGTACGCGAAAGCG
CGATCAGCGGGCTGTATCGCGCGGGCGTCGGCGTCAGCGCGTACGAGCTCGACCTGTTTGGCCGCGTGCGCAGCCTGTCC
GACGCGGCGCTCGCCGAATATTTCGCGACGGCGAACGCGCAACGCACGGTTCGCATCGGCGTGATCGCCGACGTGGCGGG
CGCCTATGTATCGGAGCGCTCGCTGCACGAGCAGCTCGCGCTGGCGCAGCGCACGCTGGATGCTCGCCAACGCATGGCCG
CGCTCACGCAGCGCCGCTATGCGGCCGGCACGAGCGACGCGATCGAGCTGCGCTCGGCCGAGATGCTGGTGGCATCCGCG
CGCGCGTCGCAGGCCGCGCTGGAGCGCGAGCATGCGCAGTCCGTGCGGGCGCTGCAGTTGCTCGCGGGCGACTTCGTGCG
CAACGTGCCCGACGACATGACCGCGCTCGACACGCTGTCGATCGCGCCGGTGGCGCCCGGCGCACCGAGTACGCTGCTCG
AACGGCGGCCGGACATCCGGCAGGCGGAGGCACGGCTCAAGGCCGCCAACGCGCAGATCGGCGCCGCGCGCGCGGCGTTC
TTCCCGCGCATCACGCTGACGAGCGACTACGGCTCGGTCAGCGACGCGTTCTCGAGCCTGTTCACCGGCGGCACGAGCGT
GTGGACGTTCGCGCCGCGCCTCACGCTGCCGATCTTCGCGGGCGGACGCAATCGCGCGAACCTCGACGTCGCGAACGCGC
GCAAGCACATCGCGGTTGCCGATTACGAGAAGACGGTGCAGACCGCGTTTCGCGAGGTGGCCGACGCATTCGCCGCGCGC
GACTGGATCGATCGCCAGCTTGCCGCGCAGCAGGACGTGTATGCGGCCGACGGCGCGCGGCTGAAGCTCGCCGAGCGTCG
CTACGCGGGCGGTGTCGCGACGTATCTCGAACTGCTCGACGCACAGCGCAGCACCTACGAGTCGGGGCAGGAGCTGATCC
GGCTCAAGCAGCTCAGGCTCGCGAATGCGATCACGTTGTACCGCGCGCTCGGCGGCGGCTGGTCGCCCGCGTCGGCGGAG
GCCGCGGCTTCCGCGTAA

Upstream 100 bases:

>100_bases
TCCTCGTCCCGCTGTTTTTCGTGATCGTCGGCCGCCTGTTCGACGTCGGCCCGCGCCGGCGCGGCGGGGCACAGCCGGCG
ACGATGGAGGGTTCTCAATA

Downstream 100 bases:

>100_bases
TGCCCGTCGCCTGACGCGACCACGCGTCGGGCGGCATCACGGCGGCCGCACGCTTCGACGTGCTGCTGCCGCTTCCTTCG
ATCTCTACCGCTGTTCCAGC

Product: RND efflux system outer membrane lipoprotein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 485; Mature: 485

Protein sequence:

>485_residues
MFALNARAALRVQVALAAALALAGCTLAPRYERPAAPVPATYAPVDGSAAPAAEVSPANAALLDDWRTYFTDAALQAWID
AALANNRDLRVAAGRLEEARALYGVQRADLMPSVDANLGYDRARQYDPVVRESAISGLYRAGVGVSAYELDLFGRVRSLS
DAALAEYFATANAQRTVRIGVIADVAGAYVSERSLHEQLALAQRTLDARQRMAALTQRRYAAGTSDAIELRSAEMLVASA
RASQAALEREHAQSVRALQLLAGDFVRNVPDDMTALDTLSIAPVAPGAPSTLLERRPDIRQAEARLKAANAQIGAARAAF
FPRITLTSDYGSVSDAFSSLFTGGTSVWTFAPRLTLPIFAGGRNRANLDVANARKHIAVADYEKTVQTAFREVADAFAAR
DWIDRQLAAQQDVYAADGARLKLAERRYAGGVATYLELLDAQRSTYESGQELIRLKQLRLANAITLYRALGGGWSPASAE
AAASA

Sequences:

>Translated_485_residues
MFALNARAALRVQVALAAALALAGCTLAPRYERPAAPVPATYAPVDGSAAPAAEVSPANAALLDDWRTYFTDAALQAWID
AALANNRDLRVAAGRLEEARALYGVQRADLMPSVDANLGYDRARQYDPVVRESAISGLYRAGVGVSAYELDLFGRVRSLS
DAALAEYFATANAQRTVRIGVIADVAGAYVSERSLHEQLALAQRTLDARQRMAALTQRRYAAGTSDAIELRSAEMLVASA
RASQAALEREHAQSVRALQLLAGDFVRNVPDDMTALDTLSIAPVAPGAPSTLLERRPDIRQAEARLKAANAQIGAARAAF
FPRITLTSDYGSVSDAFSSLFTGGTSVWTFAPRLTLPIFAGGRNRANLDVANARKHIAVADYEKTVQTAFREVADAFAAR
DWIDRQLAAQQDVYAADGARLKLAERRYAGGVATYLELLDAQRSTYESGQELIRLKQLRLANAITLYRALGGGWSPASAE
AAASA
>Mature_485_residues
MFALNARAALRVQVALAAALALAGCTLAPRYERPAAPVPATYAPVDGSAAPAAEVSPANAALLDDWRTYFTDAALQAWID
AALANNRDLRVAAGRLEEARALYGVQRADLMPSVDANLGYDRARQYDPVVRESAISGLYRAGVGVSAYELDLFGRVRSLS
DAALAEYFATANAQRTVRIGVIADVAGAYVSERSLHEQLALAQRTLDARQRMAALTQRRYAAGTSDAIELRSAEMLVASA
RASQAALEREHAQSVRALQLLAGDFVRNVPDDMTALDTLSIAPVAPGAPSTLLERRPDIRQAEARLKAANAQIGAARAAF
FPRITLTSDYGSVSDAFSSLFTGGTSVWTFAPRLTLPIFAGGRNRANLDVANARKHIAVADYEKTVQTAFREVADAFAAR
DWIDRQLAAQQDVYAADGARLKLAERRYAGGVATYLELLDAQRSTYESGQELIRLKQLRLANAITLYRALGGGWSPASAE
AAASA

Specific function: The ability to export antibiotics and solvents is dramatically decreased in the presence of the proton conductor carbonyl cyanide m-chlorophenylhydrazone (CCCP), showing that an energized inner membrane is required for efflux. It is thought that the mexB

COG id: COG1538

COG function: function code MU; Outer membrane protein

Gene ontology:

Cell location: Cell outer membrane; Lipid-anchor. Note=Attached to the outer membrane by a lipid anchor and via the transmembrane beta-barrel. The membrane anchor is not necessary for antibiotic efflux. In one report (PubMed:10889211) the Cys-18- Gly mutant is reported

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the outer membrane factor (OMF) (TC 1.B.17) family [H]

Homologues:

Organism=Escherichia coli, GI1786785, Length=459, Percent_Identity=39.8692810457516, Blast_Score=316, Evalue=2e-87,
Organism=Escherichia coli, GI2367348, Length=468, Percent_Identity=25.2136752136752, Blast_Score=96, Evalue=4e-21,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003423
- InterPro:   IPR010131 [H]

Pfam domain/function: PF02321 OEP [H]

EC number: NA

Molecular weight: Translated: 51902; Mature: 51902

Theoretical pI: Translated: 8.74; Mature: 8.74

Prosite motif: PS00013 PROKAR_LIPOPROTEIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
1.0 %Met     (Translated Protein)
1.2 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
1.0 %Met     (Mature Protein)
1.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MFALNARAALRVQVALAAALALAGCTLAPRYERPAAPVPATYAPVDGSAAPAAEVSPANA
CCCCCCCHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCH
ALLDDWRTYFTDAALQAWIDAALANNRDLRVAAGRLEEARALYGVQRADLMPSVDANLGY
HHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEHHHHHHHHHHHCCHHHHCCCCCCCCCCH
DRARQYDPVVRESAISGLYRAGVGVSAYELDLFGRVRSLSDAALAEYFATANAQRTVRIG
HHHHHCCHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEE
VIADVAGAYVSERSLHEQLALAQRTLDARQRMAALTQRRYAAGTSDAIELRSAEMLVASA
EHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHH
RASQAALEREHAQSVRALQLLAGDFVRNVPDDMTALDTLSIAPVAPGAPSTLLERRPDIR
HHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHCEECCCCCCCCHHHHHCCCCHH
QAEARLKAANAQIGAARAAFFPRITLTSDYGSVSDAFSSLFTGGTSVWTFAPRLTLPIFA
HHHHHHHHHCCHHHHHHHHHCCEEEEECCCCCHHHHHHHHHCCCCCEEEECCCEEEEEEE
GGRNRANLDVANARKHIAVADYEKTVQTAFREVADAFAARDWIDRQLAAQQDVYAADGAR
CCCCCCCCCHHHCCCCEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHCCCCE
LKLAERRYAGGVATYLELLDAQRSTYESGQELIRLKQLRLANAITLYRALGGGWSPASAE
EEEHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCH
AAASA
HHCCC
>Mature Secondary Structure
MFALNARAALRVQVALAAALALAGCTLAPRYERPAAPVPATYAPVDGSAAPAAEVSPANA
CCCCCCCHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCH
ALLDDWRTYFTDAALQAWIDAALANNRDLRVAAGRLEEARALYGVQRADLMPSVDANLGY
HHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEHHHHHHHHHHHCCHHHHCCCCCCCCCCH
DRARQYDPVVRESAISGLYRAGVGVSAYELDLFGRVRSLSDAALAEYFATANAQRTVRIG
HHHHHCCHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEE
VIADVAGAYVSERSLHEQLALAQRTLDARQRMAALTQRRYAAGTSDAIELRSAEMLVASA
EHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHH
RASQAALEREHAQSVRALQLLAGDFVRNVPDDMTALDTLSIAPVAPGAPSTLLERRPDIR
HHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHCEECCCCCCCCHHHHHCCCCHH
QAEARLKAANAQIGAARAAFFPRITLTSDYGSVSDAFSSLFTGGTSVWTFAPRLTLPIFA
HHHHHHHHHCCHHHHHHHHHCCEEEEECCCCCHHHHHHHHHCCCCCEEEECCCEEEEEEE
GGRNRANLDVANARKHIAVADYEKTVQTAFREVADAFAARDWIDRQLAAQQDVYAADGAR
CCCCCCCCCHHHCCCCEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHCCCCE
LKLAERRYAGGVATYLELLDAQRSTYESGQELIRLKQLRLANAITLYRALGGGWSPASAE
EEEHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCH
AAASA
HHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 8226684; 10889211; 10984043; 7968531; 8540696; 9401051; 9603892; 10952562 [H]