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The map label for this gene is sepA [H]

Identifier: 78066237

GI number: 78066237

Start: 1764045

End: 1765244

Strand: Direct

Name: sepA [H]

Synonym: Bcep18194_A4767

Alternate gene names: 78066237

Gene position: 1764045-1765244 (Clockwise)

Preceding gene: 78066234

Following gene: 78066238

Centisome position: 47.75

GC content: 71.67

Gene sequence:

>1200_bases
ATGAATAACAATCGCTCCCTGTTGCGCCACCGACTGGCGCCGTTCGCGCTGGCGGCCGTGCTGGCCCTGGCCGGATGTGG
AAAGGGCGACAAGGACGCCGCGCCGGAGGCCGCGAAACAGGCAACGGTCGTGACGGTGCGCCCGACGGCGGTGCCGATGA
CCGTCGAATTGCCGGGCCGGCTCGATGCTTACCGGCAGGCGGAAGTCCGCGCGCGGGTCGCGGGCATCGTGACCGCGCGC
ACCTATGAGGAAGGCCAGGAAGTGAAGCAGGGCGCGGTGCTGTTCCGCATCGATCCCGCGCCGCTGAAGGCCGCGCGCGA
CGCCGCGCAGGGCGCGCTTGCGAAGGCGCAGGCCGCCGCGCTCGCGGCGAGCGACAAGCGCCGCCGCTACGACGATCTCG
TGCGCGATCGCGCGGTGAGCGAACGCGACCATACCGAGGCCGTTGCCGGCGACACGCAGGCTAAGGCCGATGTCGCGTCA
GCGAAGGCGGAGCTCGCACGGGCACAGTTGCAGCTCGACTATGCGACCGTCACCGCGCCGATCGCGGGCCGCGCACGGCG
CGCACTCGTGACCGAAGGCGCGCTGGTCGGGCAGGACCAGGCGACGCCGCTCACGACCGTCGAGCAGCTCGATCCGATCT
ACGTGAACTTCTCGCAGCCGGCCGCCGACGTCGATGCGCTGCGCCGCGCGGTGAAGAGCGGACGTGCGACGGGCATCGCG
CAGCACGACATCGCGGTGACGCTGCTGCGCGCCGACGGCACCGCGTATCCGCTGAAGGGCAAGCTGCTGTTCAGCGATCT
CGCGGTCGATCCGACCACCGACACCGTCGCGATGCGCGCGCTGTTCCCGAATCCGGAGCGCGAATTGCTGCCGGGCGCGT
ATGTGCGGATCGCGCTCGACACGGCGGTCGACCAGCGGGCGATCCTCGTGCCGCGCGATGCGCTGCTGCGCACGGCCGAC
CGCACGTCGGTGCGGGTCGTCGGCACGAACGGCAAGGTCAAGGACGTCGAGGTCGCAGCCGACCAGATGAGCGGCCGTGA
CTGGCGCATCACGCGCGGCCTCGCGGGCGGCGAGCGCGTGATCGTCGACAACGCCGCGCAGTTCGCGCCCGATACGGCCG
TCAAGCCCGTCGAGCAGGCGCCGCCGTCGAAGGCAGCGCCGGCAGCGGCCGCTTCGCAGGCGGCTGCCCGTCAAACCTGA

Upstream 100 bases:

>100_bases
GTCAGATTGAAGTGCCGTGTATTTAATCAGTCGTGACTGATTATAATCGGCCACCCTGAGCCGCCGCATTGTATCGGTGG
CGAACGATCCGAGGTCACAC

Downstream 100 bases:

>100_bases
CTGGTTAACACCACACCACCATGGCACGTTTCTTCATCGATCGCCCCGTCTTCGCGTGGGTGATCGCAATCTTCATCATG
CTGGGCGGCGCATTCGCGAT

Product: periplasmic multidrug efflux lipoprotein precursor

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 399; Mature: 399

Protein sequence:

>399_residues
MNNNRSLLRHRLAPFALAAVLALAGCGKGDKDAAPEAAKQATVVTVRPTAVPMTVELPGRLDAYRQAEVRARVAGIVTAR
TYEEGQEVKQGAVLFRIDPAPLKAARDAAQGALAKAQAAALAASDKRRRYDDLVRDRAVSERDHTEAVAGDTQAKADVAS
AKAELARAQLQLDYATVTAPIAGRARRALVTEGALVGQDQATPLTTVEQLDPIYVNFSQPAADVDALRRAVKSGRATGIA
QHDIAVTLLRADGTAYPLKGKLLFSDLAVDPTTDTVAMRALFPNPERELLPGAYVRIALDTAVDQRAILVPRDALLRTAD
RTSVRVVGTNGKVKDVEVAADQMSGRDWRITRGLAGGERVIVDNAAQFAPDTAVKPVEQAPPSKAAPAAAASQAAARQT

Sequences:

>Translated_399_residues
MNNNRSLLRHRLAPFALAAVLALAGCGKGDKDAAPEAAKQATVVTVRPTAVPMTVELPGRLDAYRQAEVRARVAGIVTAR
TYEEGQEVKQGAVLFRIDPAPLKAARDAAQGALAKAQAAALAASDKRRRYDDLVRDRAVSERDHTEAVAGDTQAKADVAS
AKAELARAQLQLDYATVTAPIAGRARRALVTEGALVGQDQATPLTTVEQLDPIYVNFSQPAADVDALRRAVKSGRATGIA
QHDIAVTLLRADGTAYPLKGKLLFSDLAVDPTTDTVAMRALFPNPERELLPGAYVRIALDTAVDQRAILVPRDALLRTAD
RTSVRVVGTNGKVKDVEVAADQMSGRDWRITRGLAGGERVIVDNAAQFAPDTAVKPVEQAPPSKAAPAAAASQAAARQT
>Mature_399_residues
MNNNRSLLRHRLAPFALAAVLALAGCGKGDKDAAPEAAKQATVVTVRPTAVPMTVELPGRLDAYRQAEVRARVAGIVTAR
TYEEGQEVKQGAVLFRIDPAPLKAARDAAQGALAKAQAAALAASDKRRRYDDLVRDRAVSERDHTEAVAGDTQAKADVAS
AKAELARAQLQLDYATVTAPIAGRARRALVTEGALVGQDQATPLTTVEQLDPIYVNFSQPAADVDALRRAVKSGRATGIA
QHDIAVTLLRADGTAYPLKGKLLFSDLAVDPTTDTVAMRALFPNPERELLPGAYVRIALDTAVDQRAILVPRDALLRTAD
RTSVRVVGTNGKVKDVEVAADQMSGRDWRITRGLAGGERVIVDNAAQFAPDTAVKPVEQAPPSKAAPAAAASQAAARQT

Specific function: Probable periplasmic linker protein component of the sepABC efflux pump with unknown specificity [H]

COG id: COG0845

COG function: function code M; Membrane-fusion protein

Gene ontology:

Cell location: Cell inner membrane; Lipid-anchor (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the membrane fusion protein (MFP) (TC 8.A.1) family [H]

Homologues:

Organism=Escherichia coli, GI1789665, Length=381, Percent_Identity=40.6824146981627, Blast_Score=244, Evalue=6e-66,
Organism=Escherichia coli, GI1786668, Length=390, Percent_Identity=41.2820512820513, Blast_Score=242, Evalue=2e-65,
Organism=Escherichia coli, GI1789929, Length=371, Percent_Identity=37.1967654986523, Blast_Score=223, Evalue=1e-59,
Organism=Escherichia coli, GI87082036, Length=335, Percent_Identity=31.9402985074627, Blast_Score=129, Evalue=4e-31,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006143 [H]

Pfam domain/function: PF00529 HlyD [H]

EC number: NA

Molecular weight: Translated: 42145; Mature: 42145

Theoretical pI: Translated: 9.96; Mature: 9.96

Prosite motif: PS00013 PROKAR_LIPOPROTEIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
1.0 %Met     (Translated Protein)
1.3 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
1.0 %Met     (Mature Protein)
1.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNNNRSLLRHRLAPFALAAVLALAGCGKGDKDAAPEAAKQATVVTVRPTAVPMTVELPGR
CCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHCEEEEEECCCCEEEEEECCCC
LDAYRQAEVRARVAGIVTARTYEEGQEVKQGAVLFRIDPAPLKAARDAAQGALAKAQAAA
CCHHHHHHHHHHHHHHEEEHHHHHHHHHHCCEEEEEECCCHHHHHHHHHHHHHHHHHHHH
LAASDKRRRYDDLVRDRAVSERDHTEAVAGDTQAKADVASAKAELARAQLQLDYATVTAP
HHHHHHHHHHHHHHHHHHCCCCCCHHHHCCCCHHHHHHHHHHHHHHHHHHEEEEHEEECC
IAGRARRALVTEGALVGQDQATPLTTVEQLDPIYVNFSQPAADVDALRRAVKSGRATGIA
CCCHHHHHHHCCCCEECCCCCCCCCHHHHCCCEEEECCCCCHHHHHHHHHHHCCCCCCEE
QHDIAVTLLRADGTAYPLKGKLLFSDLAVDPTTDTVAMRALFPNPERELLPGAYVRIALD
ECCEEEEEEECCCCCCCCCCEEEEECCCCCCCCCCEEEEEECCCCCHHHCCCEEEEEEEE
TAVDQRAILVPRDALLRTADRTSVRVVGTNGKVKDVEVAADQMSGRDWRITRGLAGGERV
CCCCCCEEECCHHHHHHCCCCCEEEEEECCCCEEEEEEHHHHHCCCCEEEEECCCCCCEE
IVDNAAQFAPDTAVKPVEQAPPSKAAPAAAASQAAARQT
EEECCHHHCCCCCCCCHHHCCCCCCCCHHHHHHHHHCCC
>Mature Secondary Structure
MNNNRSLLRHRLAPFALAAVLALAGCGKGDKDAAPEAAKQATVVTVRPTAVPMTVELPGR
CCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHCEEEEEECCCCEEEEEECCCC
LDAYRQAEVRARVAGIVTARTYEEGQEVKQGAVLFRIDPAPLKAARDAAQGALAKAQAAA
CCHHHHHHHHHHHHHHEEEHHHHHHHHHHCCEEEEEECCCHHHHHHHHHHHHHHHHHHHH
LAASDKRRRYDDLVRDRAVSERDHTEAVAGDTQAKADVASAKAELARAQLQLDYATVTAP
HHHHHHHHHHHHHHHHHHCCCCCCHHHHCCCCHHHHHHHHHHHHHHHHHHEEEEHEEECC
IAGRARRALVTEGALVGQDQATPLTTVEQLDPIYVNFSQPAADVDALRRAVKSGRATGIA
CCCHHHHHHHCCCCEECCCCCCCCCHHHHCCCEEEECCCCCHHHHHHHHHHHCCCCCCEE
QHDIAVTLLRADGTAYPLKGKLLFSDLAVDPTTDTVAMRALFPNPERELLPGAYVRIALD
ECCEEEEEEECCCCCCCCCCEEEEECCCCCCCCCCEEEEEECCCCCHHHCCCEEEEEEEE
TAVDQRAILVPRDALLRTADRTSVRVVGTNGKVKDVEVAADQMSGRDWRITRGLAGGERV
CCCCCCEEECCHHHHHHCCCCCEEEEEECCCCEEEEEEHHHHHCCCCEEEEECCCCCCEE
IVDNAAQFAPDTAVKPVEQAPPSKAAPAAAASQAAARQT
EEECCHHHCCCCCCCCHHHCCCCCCCCHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 9037074 [H]