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The map label for this gene is rlmB [H]

Identifier: 78066159

GI number: 78066159

Start: 1686025

End: 1686768

Strand: Direct

Name: rlmB [H]

Synonym: Bcep18194_A4689

Alternate gene names: 78066159

Gene position: 1686025-1686768 (Clockwise)

Preceding gene: 78066158

Following gene: 78066160

Centisome position: 45.64

GC content: 68.68

Gene sequence:

>744_bases
ATGTCACGTCTGAAGGTTCTTTACGGTTTTCATGCGGTGACCGCACGTTTGCGGCACGATGCATCGACGGTTGCGGAGGT
GCTGTACGACCAGACGCGCCGTGACCGCCGGATGCAGGACTTCCTGCACACCGCGAAGGAAGCGGGCGTGCGGCTGATCG
CGGCCGACGAAACGCGCCTGTGGGGCCTCGCGCATACCGAGCGTCACCAGGGCGTCGTCGCGCGTGTCGAGGACATCCCG
CTCGCACAGAACCTGTCGGAGCTGCTCGACGGCATCCAGGGCCCGGCGCTGCTGCTGGTGCTCGACGGCGTCACCGATCC
GCACAACCTCGGCGCGTGCCTGCGTGTCGCCGATTCGGCCGGCGCGCACGCGGTGATCGCGCCGCGCGACCGTGCGGTCG
GCCTCAACGCCACCGCGGCCAAGGTCGCGAGCGGCGCGGCCGATACGGTGCCGTACATCACGGTGACGAACCTCGCCCGC
GCGCTGCGCGAGCTGAAGGAGGCCGGCGTGTGGATCATCGGCACGTCGGACGAGGCATCGGCAACGCTCTACGAAACGAA
GCTCGACGGCCCCGTCGCGCTCGTGATGGGCGCGGAAGGCGAAGGCATGCGCCGGCTGACGCGCGACACCTGCGACGAAG
TGATGAGCATTCCGATGGCCGGCAGCGTGGAAAGCCTGAACGTATCGGTCGCAAGCGGCGTGTGCCTGTACGAAGCCGTG
CGCCAGCGGCGCGTGAAGGGCTGA

Upstream 100 bases:

>100_bases
CGTCACCGGCCATCAGCCGGCAACGCGGCGCTTTCCTTTTCCATGGATCGCGGCTGCGCGCGTCGCGCGGTCGCACGTTT
GATCGAAGGTTGTTCCAGTC

Downstream 100 bases:

>100_bases
CACAGCCCGTCCCGCGCGCCCTGCGCGGGCCTCGAATGACCCGAACGACGCGTGCCGCGATCCGATGTGGCACGCGGCTC
TCCGGAGCTCGCGACCGATG

Product: RNA methyltransferase TrmH, group 3

Products: NA

Alternate protein names: 23S rRNA Gm2251 2'-O-methyltransferase [H]

Number of amino acids: Translated: 247; Mature: 246

Protein sequence:

>247_residues
MSRLKVLYGFHAVTARLRHDASTVAEVLYDQTRRDRRMQDFLHTAKEAGVRLIAADETRLWGLAHTERHQGVVARVEDIP
LAQNLSELLDGIQGPALLLVLDGVTDPHNLGACLRVADSAGAHAVIAPRDRAVGLNATAAKVASGAADTVPYITVTNLAR
ALRELKEAGVWIIGTSDEASATLYETKLDGPVALVMGAEGEGMRRLTRDTCDEVMSIPMAGSVESLNVSVASGVCLYEAV
RQRRVKG

Sequences:

>Translated_247_residues
MSRLKVLYGFHAVTARLRHDASTVAEVLYDQTRRDRRMQDFLHTAKEAGVRLIAADETRLWGLAHTERHQGVVARVEDIP
LAQNLSELLDGIQGPALLLVLDGVTDPHNLGACLRVADSAGAHAVIAPRDRAVGLNATAAKVASGAADTVPYITVTNLAR
ALRELKEAGVWIIGTSDEASATLYETKLDGPVALVMGAEGEGMRRLTRDTCDEVMSIPMAGSVESLNVSVASGVCLYEAV
RQRRVKG
>Mature_246_residues
SRLKVLYGFHAVTARLRHDASTVAEVLYDQTRRDRRMQDFLHTAKEAGVRLIAADETRLWGLAHTERHQGVVARVEDIPL
AQNLSELLDGIQGPALLLVLDGVTDPHNLGACLRVADSAGAHAVIAPRDRAVGLNATAAKVASGAADTVPYITVTNLARA
LRELKEAGVWIIGTSDEASATLYETKLDGPVALVMGAEGEGMRRLTRDTCDEVMSIPMAGSVESLNVSVASGVCLYEAVR
QRRVKG

Specific function: Specifically methylates the ribose of guanosine 2251 in 23S rRNA [H]

COG id: COG0566

COG function: function code J; rRNA methylases

Gene ontology:

Cell location: Cytoplasm (Potential) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the RNA methyltransferase TrmH family. RlmB subfamily [H]

Homologues:

Organism=Homo sapiens, GI40068479, Length=263, Percent_Identity=26.9961977186312, Blast_Score=82, Evalue=5e-16,
Organism=Escherichia coli, GI1790623, Length=241, Percent_Identity=54.7717842323651, Blast_Score=256, Evalue=1e-69,
Organism=Escherichia coli, GI1788935, Length=192, Percent_Identity=33.3333333333333, Blast_Score=87, Evalue=1e-18,
Organism=Escherichia coli, GI1790083, Length=157, Percent_Identity=36.9426751592357, Blast_Score=81, Evalue=6e-17,
Organism=Caenorhabditis elegans, GI17555908, Length=256, Percent_Identity=25.390625, Blast_Score=71, Evalue=5e-13,
Organism=Saccharomyces cerevisiae, GI6324775, Length=184, Percent_Identity=30.9782608695652, Blast_Score=74, Evalue=3e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004441
- InterPro:   IPR001537
- InterPro:   IPR013123 [H]

Pfam domain/function: PF00588 SpoU_methylase; PF08032 SpoU_sub_bind [H]

EC number: 2.1.1.- [C]

Molecular weight: Translated: 26503; Mature: 26371

Theoretical pI: Translated: 6.63; Mature: 6.63

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSRLKVLYGFHAVTARLRHDASTVAEVLYDQTRRDRRMQDFLHTAKEAGVRLIAADETRL
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCEEEEEECCCEE
WGLAHTERHQGVVARVEDIPLAQNLSELLDGIQGPALLLVLDGVTDPHNLGACLRVADSA
EECCHHHHHCCCEEECCCCCCHHHHHHHHHCCCCCEEEEEEECCCCCHHHHHHHEECCCC
GAHAVIAPRDRAVGLNATAAKVASGAADTVPYITVTNLARALRELKEAGVWIIGTSDEAS
CCEEEECCCCCCCCCCHHHHHHHCCCCCCCCEEEHHHHHHHHHHHHHCCEEEEECCCCCC
ATLYETKLDGPVALVMGAEGEGMRRLTRDTCDEVMSIPMAGSVESLNVSVASGVCLYEAV
EEEEEEECCCCEEEEECCCCCHHHHHHHHHHHHHHHCCCCCCCHHCEEHHHCCHHHHHHH
RQRRVKG
HHHCCCC
>Mature Secondary Structure 
SRLKVLYGFHAVTARLRHDASTVAEVLYDQTRRDRRMQDFLHTAKEAGVRLIAADETRL
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCEEEEEECCCEE
WGLAHTERHQGVVARVEDIPLAQNLSELLDGIQGPALLLVLDGVTDPHNLGACLRVADSA
EECCHHHHHCCCEEECCCCCCHHHHHHHHHCCCCCEEEEEEECCCCCHHHHHHHEECCCC
GAHAVIAPRDRAVGLNATAAKVASGAADTVPYITVTNLARALRELKEAGVWIIGTSDEAS
CCEEEECCCCCCCCCCHHHHHHHCCCCCCCCEEEHHHHHHHHHHHHHCCEEEEECCCCCC
ATLYETKLDGPVALVMGAEGEGMRRLTRDTCDEVMSIPMAGSVESLNVSVASGVCLYEAV
EEEEEEECCCCEEEEECCCCCHHHHHHHHHHHHHHHCCCCCCCHHCEEHHHCCHHHHHHH
RQRRVKG
HHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11823852 [H]