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The map label for this gene is amiD [H]

Identifier: 78066160

GI number: 78066160

Start: 1686866

End: 1687738

Strand: Direct

Name: amiD [H]

Synonym: Bcep18194_A4690

Alternate gene names: 78066160

Gene position: 1686866-1687738 (Clockwise)

Preceding gene: 78066159

Following gene: 78066161

Centisome position: 45.66

GC content: 68.73

Gene sequence:

>873_bases
ATGACCCTGTTACGCGTTGGTGCATCCTGCGCCGTGCTGTGCCTGCTGGCCGCCTGCACGTCGCCGTCGCTCGTCGAACG
CGGCACCTACTACGCCGACACGAGCCTGCACGCGCGCGGCGCCGATTCGCGGGTCCGCTTTCTCGTGATGCATTACACCG
AAAGCGACGAGGCGAAATCGCTGCGCACGCTGACGGGCGATTCGGTCAGCGTGCACTACGTCATCCCGCCGCAGGCGCGT
ATCGAGCGCGGGATGCCGGTCGTCTACCAGCTCGTTCCGGAATCGGAGCGCGCGTGGCACGCGGGCGTCAGCGAGTGGCA
GGGCACGACCGAGTTGAACGCGGTGTCGATCGGCATCGAGAACGTGAACCGCGGGCCGCTTGATCCGCAAAACCGCACCT
GGCAGCCTTATCCGCCCGAACAGGTCGCGGCGCTGACCCGGCTGTCGAAGGACATCGTCGCGCGCTACGGGATTCCGCCG
ACGCGCGTGGTCGGACACAGCGACATCGCGCCACAACGCAAGATCGATCCGGGGCCGCTGTTTCCGTGGCATGCGCTCGC
CCAGGCCGGCGTCGGCGCGTGGCCGGACGATGCGACCGTGACCGCGCGGCTCGGCGGTCGTGATCCGCATGCGCCGGTCG
ACGTGCGCGAACTGCAGCTCAAGCTCGCGCGCTACGGTTACGACTTGGCCACCGATGGCGTGCTCGACGCCCGCACGCGA
CGCGTGTTCGCGGCTTTCCAGATGCATTTCAGGCCGTCCGGCTATGCGGGCAACCCGGACGCCGAAACCGACGCAATCGC
GCAGGCGTTGCTCGACAAGTACTTCCCCGGCACGCAACCGGCGGACAACGCGCCCGAGGCGGGCGCGCCCTGA

Upstream 100 bases:

>100_bases
TGACACAGCCCGTCCCGCGCGCCCTGCGCGGGCCTCGAATGACCCGAACGACGCGTGCCGCGATCCGATGTGGCACGCGG
CTCTCCGGAGCTCGCGACCG

Downstream 100 bases:

>100_bases
GTCCACAGCGCACGGCACCCCGAAAAACGCGGGGCGGTCATCCGAGTCCGGTAAACTGGCGGTTTTCCGCAATCCCGCAG
CATTACCACTCCATGACTCA

Product: N-acetylmuramyl-L-alanine amidase, negative regulator of AmpC, AmpD

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 290; Mature: 289

Protein sequence:

>290_residues
MTLLRVGASCAVLCLLAACTSPSLVERGTYYADTSLHARGADSRVRFLVMHYTESDEAKSLRTLTGDSVSVHYVIPPQAR
IERGMPVVYQLVPESERAWHAGVSEWQGTTELNAVSIGIENVNRGPLDPQNRTWQPYPPEQVAALTRLSKDIVARYGIPP
TRVVGHSDIAPQRKIDPGPLFPWHALAQAGVGAWPDDATVTARLGGRDPHAPVDVRELQLKLARYGYDLATDGVLDARTR
RVFAAFQMHFRPSGYAGNPDAETDAIAQALLDKYFPGTQPADNAPEAGAP

Sequences:

>Translated_290_residues
MTLLRVGASCAVLCLLAACTSPSLVERGTYYADTSLHARGADSRVRFLVMHYTESDEAKSLRTLTGDSVSVHYVIPPQAR
IERGMPVVYQLVPESERAWHAGVSEWQGTTELNAVSIGIENVNRGPLDPQNRTWQPYPPEQVAALTRLSKDIVARYGIPP
TRVVGHSDIAPQRKIDPGPLFPWHALAQAGVGAWPDDATVTARLGGRDPHAPVDVRELQLKLARYGYDLATDGVLDARTR
RVFAAFQMHFRPSGYAGNPDAETDAIAQALLDKYFPGTQPADNAPEAGAP
>Mature_289_residues
TLLRVGASCAVLCLLAACTSPSLVERGTYYADTSLHARGADSRVRFLVMHYTESDEAKSLRTLTGDSVSVHYVIPPQARI
ERGMPVVYQLVPESERAWHAGVSEWQGTTELNAVSIGIENVNRGPLDPQNRTWQPYPPEQVAALTRLSKDIVARYGIPPT
RVVGHSDIAPQRKIDPGPLFPWHALAQAGVGAWPDDATVTARLGGRDPHAPVDVRELQLKLARYGYDLATDGVLDARTRR
VFAAFQMHFRPSGYAGNPDAETDAIAQALLDKYFPGTQPADNAPEAGAP

Specific function: Unknown

COG id: COG3023

COG function: function code V; Negative regulator of beta-lactamase expression

Gene ontology:

Cell location: Cell outer membrane; Lipid-anchor (Probable) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the N-acetylmuramoyl-L-alanine amidase 2 family [H]

Homologues:

Organism=Escherichia coli, GI1787092, Length=252, Percent_Identity=53.5714285714286, Blast_Score=253, Evalue=9e-69,
Organism=Escherichia coli, GI1786300, Length=112, Percent_Identity=41.0714285714286, Blast_Score=71, Evalue=1e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002502
- InterPro:   IPR002477 [H]

Pfam domain/function: PF01510 Amidase_2 [H]

EC number: =3.5.1.28 [H]

Molecular weight: Translated: 31545; Mature: 31414

Theoretical pI: Translated: 6.51; Mature: 6.51

Prosite motif: PS00013 PROKAR_LIPOPROTEIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
1.4 %Met     (Translated Protein)
2.4 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
1.0 %Met     (Mature Protein)
2.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTLLRVGASCAVLCLLAACTSPSLVERGTYYADTSLHARGADSRVRFLVMHYTESDEAKS
CCEEECCHHHHHHHHHHHCCCCHHHHCCCEEECCCEECCCCCCEEEEEEEEECCCCHHHH
LRTLTGDSVSVHYVIPPQARIERGMPVVYQLVPESERAWHAGVSEWQGTTELNAVSIGIE
HHHCCCCCEEEEEEECCHHHHHCCCCEEEEECCCCCHHHHCCHHHCCCCCCCEEEEEECC
NVNRGPLDPQNRTWQPYPPEQVAALTRLSKDIVARYGIPPTRVVGHSDIAPQRKIDPGPL
CCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCHHEECCCCCCCCCCCCCCCC
FPWHALAQAGVGAWPDDATVTARLGGRDPHAPVDVRELQLKLARYGYDLATDGVLDARTR
CHHHHHHHCCCCCCCCCCEEEEEECCCCCCCCCCHHHHHHHHHHHCCCCCCCCCHHHHHH
RVFAAFQMHFRPSGYAGNPDAETDAIAQALLDKYFPGTQPADNAPEAGAP
HHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHCCCCCCCCCCCCCCCC
>Mature Secondary Structure 
TLLRVGASCAVLCLLAACTSPSLVERGTYYADTSLHARGADSRVRFLVMHYTESDEAKS
CEEECCHHHHHHHHHHHCCCCHHHHCCCEEECCCEECCCCCCEEEEEEEEECCCCHHHH
LRTLTGDSVSVHYVIPPQARIERGMPVVYQLVPESERAWHAGVSEWQGTTELNAVSIGIE
HHHCCCCCEEEEEEECCHHHHHCCCCEEEEECCCCCHHHHCCHHHCCCCCCCEEEEEECC
NVNRGPLDPQNRTWQPYPPEQVAALTRLSKDIVARYGIPPTRVVGHSDIAPQRKIDPGPL
CCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCCCHHEECCCCCCCCCCCCCCCC
FPWHALAQAGVGAWPDDATVTARLGGRDPHAPVDVRELQLKLARYGYDLATDGVLDARTR
CHHHHHHHCCCCCCCCCCEEEEEECCCCCCCCCCHHHHHHHHHHHCCCCCCCCCHHHHHH
RVFAAFQMHFRPSGYAGNPDAETDAIAQALLDKYFPGTQPADNAPEAGAP
HHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHCCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 8905232; 9278503 [H]