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The map label for this gene is sucB [H]

Identifier: 78066120

GI number: 78066120

Start: 1638518

End: 1639792

Strand: Direct

Name: sucB [H]

Synonym: Bcep18194_A4650

Alternate gene names: 78066120

Gene position: 1638518-1639792 (Clockwise)

Preceding gene: 78066119

Following gene: 78066121

Centisome position: 44.35

GC content: 64.55

Gene sequence:

>1275_bases
ATGGCTATCGTAGAAGTCAAAGTCCCCCAGCTTTCGGAGTCGGTTTCGGAAGCCACCATGCTGCAGTGGAAGAAGAAGCC
GGGCGAAGCAGTTGCTCAAGACGAAATCCTGATCGAACTCGAGACCGACAAGGTCGTGCTCGAAGTGCCGGCACCGGCAG
CGGGCGTGCTCGCGCAAGTGCTGCAGAACGACGGTGACACCGTCGTTGCCGATCAGATCATCGCGACGATCGACACCGAA
GCGAAGGCGGGTGCAGCCGAAGCCGCCGCAGGCGCCGCCGAAGTCAAGCCGGCAGCAGCGCCTGCCGCAGCCGCACCGGC
AGCACAGCCGGTCGCCGCAGCCGCATCGTCGACGACCGCATCGCCGGCCGCATCGAAGCTGCTGGCCGAGAAGGGCCTGT
CGGCGGGCGACGTCGCAGGTTCGGGCCGTGACGGCCGCGTCACGAAGGGCGACGCGCTGGCAGCAGGCAGCGCACCGAAG
GCCGCTCCGGCTGCCGCACCGGCCAAGACGGCCGCTGCGAAGCCGGCGCTGCCGGAAGTGAAGGTGCCGGCATCGGCCGC
GACCTGGCTGAACGACCGTCCGGAACAGCGCGTGCCGATGTCGCGCCTGCGTGCGCGTATCGCCGAGCGTCTGCTCGAGT
CGCAGCAGACCAACGCGATCCTGACGACGTTCAACGAAGTGAACATGGCTCCGGTCATGGAACTGCGTAACAAGTACAAG
GACAAGTTCGAGAAGGAACATGGCGTGAAGCTCGGCTTCATGTCGTTCTTCGTGAAGGCAGCTGTCCACGCGCTGAAGAA
GTTCCCGCTCGTGAACGCGTCGATCGACGGTAACGACATCGTCTACCACGGCTACTTCGACATCGGTATCGCTGTCGGCT
CGCCGCGCGGCCTCGTCGTGCCGATCCTGCGCAACGCGGATCAACTGAGCCTCGCCGAGATCGAAAAGAAGATCGCCGAG
TTCGGCCAGAAGGCGAAGGACGGCAAGCTGTCGATCGAGGAAATGACGGGCGGTACGTTCTCGATCTCGAACGGCGGTGT
GTTCGGCTCGATGCTGTCGACCCCGATCATCAACCCGCCGCAGTCGGCCATCCTCGGCGTGCACGCGACGAAGGAGCGTC
CGGTCGTCGAAAACGGCCAGATCGTGATCCGTCCGATCAACTACCTCGCGCTGTCGTACGACCACCGCATCATCGACGGC
CGCGAAGCCGTGCTGTCGCTCGTCGCGATGAAGGATGCGCTGGAAGATCCGGCACGTCTGCTGCTCGACCTGTAA

Upstream 100 bases:

>100_bases
TCCCGTCTCTTTTGGCCCGCCGGGCGCATTGCGCGCCGCACCGGCCGAAGGAATCGCACGAACCTCGTCATTACCCAGAT
TAAGCATCCAGGAAAATCAC

Downstream 100 bases:

>100_bases
GCCGAGTTTCACTGCATTGACCCGCACCGCACGGGCGCGCCACGCGCGTGGCCCGTGCGGCTGCACAAGAAGAAAGGATT
GTCATGTCCAAGGAATTTGA

Product: dihydrolipoamide succinyltransferase

Products: NA

Alternate protein names: 2-oxoglutarate dehydrogenase complex component E2; OGDC-E2; Dihydrolipoamide succinyltransferase component of 2-oxoglutarate dehydrogenase complex [H]

Number of amino acids: Translated: 424; Mature: 423

Protein sequence:

>424_residues
MAIVEVKVPQLSESVSEATMLQWKKKPGEAVAQDEILIELETDKVVLEVPAPAAGVLAQVLQNDGDTVVADQIIATIDTE
AKAGAAEAAAGAAEVKPAAAPAAAAPAAQPVAAAASSTTASPAASKLLAEKGLSAGDVAGSGRDGRVTKGDALAAGSAPK
AAPAAAPAKTAAAKPALPEVKVPASAATWLNDRPEQRVPMSRLRARIAERLLESQQTNAILTTFNEVNMAPVMELRNKYK
DKFEKEHGVKLGFMSFFVKAAVHALKKFPLVNASIDGNDIVYHGYFDIGIAVGSPRGLVVPILRNADQLSLAEIEKKIAE
FGQKAKDGKLSIEEMTGGTFSISNGGVFGSMLSTPIINPPQSAILGVHATKERPVVENGQIVIRPINYLALSYDHRIIDG
REAVLSLVAMKDALEDPARLLLDL

Sequences:

>Translated_424_residues
MAIVEVKVPQLSESVSEATMLQWKKKPGEAVAQDEILIELETDKVVLEVPAPAAGVLAQVLQNDGDTVVADQIIATIDTE
AKAGAAEAAAGAAEVKPAAAPAAAAPAAQPVAAAASSTTASPAASKLLAEKGLSAGDVAGSGRDGRVTKGDALAAGSAPK
AAPAAAPAKTAAAKPALPEVKVPASAATWLNDRPEQRVPMSRLRARIAERLLESQQTNAILTTFNEVNMAPVMELRNKYK
DKFEKEHGVKLGFMSFFVKAAVHALKKFPLVNASIDGNDIVYHGYFDIGIAVGSPRGLVVPILRNADQLSLAEIEKKIAE
FGQKAKDGKLSIEEMTGGTFSISNGGVFGSMLSTPIINPPQSAILGVHATKERPVVENGQIVIRPINYLALSYDHRIIDG
REAVLSLVAMKDALEDPARLLLDL
>Mature_423_residues
AIVEVKVPQLSESVSEATMLQWKKKPGEAVAQDEILIELETDKVVLEVPAPAAGVLAQVLQNDGDTVVADQIIATIDTEA
KAGAAEAAAGAAEVKPAAAPAAAAPAAQPVAAAASSTTASPAASKLLAEKGLSAGDVAGSGRDGRVTKGDALAAGSAPKA
APAAAPAKTAAAKPALPEVKVPASAATWLNDRPEQRVPMSRLRARIAERLLESQQTNAILTTFNEVNMAPVMELRNKYKD
KFEKEHGVKLGFMSFFVKAAVHALKKFPLVNASIDGNDIVYHGYFDIGIAVGSPRGLVVPILRNADQLSLAEIEKKIAEF
GQKAKDGKLSIEEMTGGTFSISNGGVFGSMLSTPIINPPQSAILGVHATKERPVVENGQIVIRPINYLALSYDHRIIDGR
EAVLSLVAMKDALEDPARLLLDL

Specific function: The 2-oxoglutarate dehydrogenase complex catalyzes the overall conversion of 2-oxoglutarate to succinyl-CoA and CO(2). It contains multiple copies of three enzymatic components:2- oxoglutarate dehydrogenase (E1), dihydrolipoamide succinyltransferase (E2)

COG id: COG0508

COG function: function code C; Pyruvate/2-oxoglutarate dehydrogenase complex, dihydrolipoamide acyltransferase (E2) component, and related enzymes

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 lipoyl-binding domain [H]

Homologues:

Organism=Homo sapiens, GI31711992, Length=443, Percent_Identity=30.0225733634311, Blast_Score=172, Evalue=4e-43,
Organism=Homo sapiens, GI110671329, Length=435, Percent_Identity=28.0459770114943, Blast_Score=165, Evalue=7e-41,
Organism=Homo sapiens, GI203098753, Length=458, Percent_Identity=29.4759825327511, Blast_Score=159, Evalue=3e-39,
Organism=Homo sapiens, GI203098816, Length=458, Percent_Identity=29.4759825327511, Blast_Score=159, Evalue=4e-39,
Organism=Homo sapiens, GI260898739, Length=161, Percent_Identity=37.888198757764, Blast_Score=100, Evalue=2e-21,
Organism=Escherichia coli, GI1786946, Length=427, Percent_Identity=53.1615925058548, Blast_Score=438, Evalue=1e-124,
Organism=Escherichia coli, GI1786305, Length=430, Percent_Identity=31.8604651162791, Blast_Score=169, Evalue=3e-43,
Organism=Caenorhabditis elegans, GI25146366, Length=429, Percent_Identity=43.1235431235431, Blast_Score=328, Evalue=3e-90,
Organism=Caenorhabditis elegans, GI17560088, Length=452, Percent_Identity=31.1946902654867, Blast_Score=177, Evalue=1e-44,
Organism=Caenorhabditis elegans, GI17537937, Length=443, Percent_Identity=26.1851015801354, Blast_Score=162, Evalue=4e-40,
Organism=Caenorhabditis elegans, GI17538894, Length=316, Percent_Identity=32.9113924050633, Blast_Score=141, Evalue=7e-34,
Organism=Saccharomyces cerevisiae, GI6320352, Length=419, Percent_Identity=43.9140811455847, Blast_Score=331, Evalue=1e-91,
Organism=Saccharomyces cerevisiae, GI6324258, Length=454, Percent_Identity=30.3964757709251, Blast_Score=159, Evalue=1e-39,
Organism=Drosophila melanogaster, GI24645909, Length=242, Percent_Identity=54.9586776859504, Blast_Score=283, Evalue=2e-76,
Organism=Drosophila melanogaster, GI18859875, Length=435, Percent_Identity=28.9655172413793, Blast_Score=155, Evalue=4e-38,
Organism=Drosophila melanogaster, GI20129315, Length=228, Percent_Identity=34.2105263157895, Blast_Score=130, Evalue=2e-30,
Organism=Drosophila melanogaster, GI24582497, Length=228, Percent_Identity=34.2105263157895, Blast_Score=130, Evalue=2e-30,

Paralogues:

None

Copy number: 420 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 3096 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 3,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003016
- InterPro:   IPR001078
- InterPro:   IPR000089
- InterPro:   IPR023213
- InterPro:   IPR004167
- InterPro:   IPR011053
- InterPro:   IPR006255 [H]

Pfam domain/function: PF00198 2-oxoacid_dh; PF00364 Biotin_lipoyl; PF02817 E3_binding [H]

EC number: =2.3.1.61 [H]

Molecular weight: Translated: 44287; Mature: 44156

Theoretical pI: Translated: 5.80; Mature: 5.80

Prosite motif: PS50968 BIOTINYL_LIPOYL ; PS00189 LIPOYL

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
2.1 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
1.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAIVEVKVPQLSESVSEATMLQWKKKPGEAVAQDEILIELETDKVVLEVPAPAAGVLAQV
CEEEEEECCHHHHHHHHHHHHHHHCCCCCCCCCCCEEEEEECCEEEEECCCCHHHHHHHH
LQNDGDTVVADQIIATIDTEAKAGAAEAAAGAAEVKPAAAPAAAAPAAQPVAAAASSTTA
HHCCCCEEEHHHHHHHCCCCCCCCCHHHHCCCHHCCCCCCCCCCCCCCCCHHHHCCCCCC
SPAASKLLAEKGLSAGDVAGSGRDGRVTKGDALAAGSAPKAAPAAAPAKTAAAKPALPEV
CHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCEECCCCCCCCCCCCCCHHHCCCCCCCCC
KVPASAATWLNDRPEQRVPMSRLRARIAERLLESQQTNAILTTFNEVNMAPVMELRNKYK
CCCCHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHCCCEEEEECCCCCCHHHHHHHHHHH
DKFEKEHGVKLGFMSFFVKAAVHALKKFPLVNASIDGNDIVYHGYFDIGIAVGSPRGLVV
HHHHHHHCCCHHHHHHHHHHHHHHHHHCCCEECCCCCCEEEEEEEEEEEEEECCCCCEEE
PILRNADQLSLAEIEKKIAEFGQKAKDGKLSIEEMTGGTFSISNGGVFGSMLSTPIINPP
EEECCCCCHHHHHHHHHHHHHCCCCCCCCEEEEECCCCEEEECCCCEEHHHHCCCCCCCC
QSAILGVHATKERPVVENGQIVIRPINYLALSYDHRIIDGREAVLSLVAMKDALEDPARL
HHHEEEEECCCCCCCCCCCCEEEEECCEEEEECCCEEECCHHHHHHHHHHHHHHCCHHHH
LLDL
HHCC
>Mature Secondary Structure 
AIVEVKVPQLSESVSEATMLQWKKKPGEAVAQDEILIELETDKVVLEVPAPAAGVLAQV
EEEEEECCHHHHHHHHHHHHHHHCCCCCCCCCCCEEEEEECCEEEEECCCCHHHHHHHH
LQNDGDTVVADQIIATIDTEAKAGAAEAAAGAAEVKPAAAPAAAAPAAQPVAAAASSTTA
HHCCCCEEEHHHHHHHCCCCCCCCCHHHHCCCHHCCCCCCCCCCCCCCCCHHHHCCCCCC
SPAASKLLAEKGLSAGDVAGSGRDGRVTKGDALAAGSAPKAAPAAAPAKTAAAKPALPEV
CHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCEECCCCCCCCCCCCCCHHHCCCCCCCCC
KVPASAATWLNDRPEQRVPMSRLRARIAERLLESQQTNAILTTFNEVNMAPVMELRNKYK
CCCCHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHCCCEEEEECCCCCCHHHHHHHHHHH
DKFEKEHGVKLGFMSFFVKAAVHALKKFPLVNASIDGNDIVYHGYFDIGIAVGSPRGLVV
HHHHHHHCCCHHHHHHHHHHHHHHHHHCCCEECCCCCCEEEEEEEEEEEEEECCCCCEEE
PILRNADQLSLAEIEKKIAEFGQKAKDGKLSIEEMTGGTFSISNGGVFGSMLSTPIINPP
EEECCCCCHHHHHHHHHHHHHCCCCCCCCEEEEECCCCEEEECCCCEEHHHHCCCCCCCC
QSAILGVHATKERPVVENGQIVIRPINYLALSYDHRIIDGREAVLSLVAMKDALEDPARL
HHHEEEEECCCCCCCCCCCCEEEEECCEEEEECCCEEECCHHHHHHHHHHHHHHCCHHHH
LLDL
HHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8867378 [H]