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The map label for this gene is odhL [H]

Identifier: 78066121

GI number: 78066121

Start: 1639876

End: 1641306

Strand: Direct

Name: odhL [H]

Synonym: Bcep18194_A4651

Alternate gene names: 78066121

Gene position: 1639876-1641306 (Clockwise)

Preceding gene: 78066120

Following gene: 78066122

Centisome position: 44.39

GC content: 64.57

Gene sequence:

>1431_bases
ATGTCCAAGGAATTTGACGTCGTCGTGATCGGCGCCGGCCCCGGCGGCTACATCGCCGCGATCCGCGCCGCGCAGCTCGG
CAAGACCGTTGCCTGTATCGAGAAGTGGAAGAACCCGGCCGGCGCGCTGAAGCTTGGCGGTACGTGCCTGAACGTCGGCT
GCATTCCGTCGAAGGCGCTGCTCGCGTCGTCGGAAGAGTTCGAGAACACGTCGCACCACCTGGCCGACCACGGCATCACG
GTCGACGGCGTGAAGATCGACGTCGCGAAGATGCTCGGCCGCAAGGACGCGATCGTCGAGAAGATGACGAGCGGGATCGA
GTTCCTGTTCAAGAAGAACAAGATCACCTGGCTGAAGGGCCATGGCAAGTTCACCGGCAAGACCGACGCCGGCGTGCAGA
TCGAAGTGAGCGGCGAAGGTGAAACCGAAGTCGTCACCGCGAAGAACGTGATCATTGCGACGGGCTCGAAGGCGCGTCAC
CTGCCGGGCATCCCGGTCGACAACAAGATCGTGTCGGACAACGAAGGTGCACTGACGTTCGACGCGGTGCCGAAGAAGCT
TGCCGTGATCGGCGCAGGCGTGATCGGCCTCGAGCTCGGCTCGGTGTGGCGTCGCCTGGGTGCCGAAGTGACGGTGCTCG
AAGCGCTGCCGGCCTTCCTCGGCGCAGCTGACGAAGCGCTCGCGAAGGAAGCGGCCAAGCTGTTCAAGAAGCAGGGCCTC
GACATCAACCTCGGCGTGCAGATCGGCGAAGTGAAGGCGACCGCGAACGGCGTGTCGATCGCCTACACGGACAAGGACGG
CAACGCGCAGACGCTCGACGCCGACCGCCTGATCGTGTCGGTCGGCCGCGTGCCGAACACCGACAACCTCGGCCTCGAGG
CTATCGGCCTGAAGGCGAACGAACGCGGCTTCATCGACGTGGACGACCACTGCCGCACGGCGGTGCCGAACGTGTACGCG
ATCGGCGACGTGGTGCGTGGCCCGATGCTCGCGCACAAGGCGGAAGACGAAGGCGTGCTGGTCGCGGAAGTGATCGATGG
CCAGAAGCCGCACATCGACTACAACTGCATTCCGTGGGTGATCTACACGTACCCGGAAATCGCATGGGTCGGCAAGACGG
AGCAGCAGCTGAAGGCGGAAGGCCGCGAGATCAAGTCGGGCAAGTTCCCGTTCTCGATCAACGGCCGCGCGCTCGGCATG
AACGCGCCGGACGGCTTCGTGAAGATGATCGCGGACGCGAAGACCGACGAACTGCTCGGCGTGCACGTGATCGCGGCGAA
CGCGTCGGACCTGATCGCGGAAGCCGTGGTGGCGATGGAATTCAAGGCGGCGTCGGAAGACATCGCCCGCATCTGCCATC
CGCACCCGTCGATGTCGGAAGTGATGCGCGAAGCGGCGCTCGCCGTCGACAAGCGCTCGCTGAACAGCTGA

Upstream 100 bases:

>100_bases
TGCTGCTCGACCTGTAAGCCGAGTTTCACTGCATTGACCCGCACCGCACGGGCGCGCCACGCGCGTGGCCCGTGCGGCTG
CACAAGAAGAAAGGATTGTC

Downstream 100 bases:

>100_bases
GCGCGGTGTAGCGCCGGCCGGCAATCGGCCAGCGCAGCCGTCTCATGACGAGGGCGGGCGGGATTTCCCGTCCGCCTTCG
TTTTTTCCGGTTTGCCCGAT

Product: dihydrolipoamide dehydrogenase

Products: NA

Alternate protein names: Dihydrolipoamide dehydrogenase; E3 component of 2-oxoglutarate dehydrogenase complex [H]

Number of amino acids: Translated: 476; Mature: 475

Protein sequence:

>476_residues
MSKEFDVVVIGAGPGGYIAAIRAAQLGKTVACIEKWKNPAGALKLGGTCLNVGCIPSKALLASSEEFENTSHHLADHGIT
VDGVKIDVAKMLGRKDAIVEKMTSGIEFLFKKNKITWLKGHGKFTGKTDAGVQIEVSGEGETEVVTAKNVIIATGSKARH
LPGIPVDNKIVSDNEGALTFDAVPKKLAVIGAGVIGLELGSVWRRLGAEVTVLEALPAFLGAADEALAKEAAKLFKKQGL
DINLGVQIGEVKATANGVSIAYTDKDGNAQTLDADRLIVSVGRVPNTDNLGLEAIGLKANERGFIDVDDHCRTAVPNVYA
IGDVVRGPMLAHKAEDEGVLVAEVIDGQKPHIDYNCIPWVIYTYPEIAWVGKTEQQLKAEGREIKSGKFPFSINGRALGM
NAPDGFVKMIADAKTDELLGVHVIAANASDLIAEAVVAMEFKAASEDIARICHPHPSMSEVMREAALAVDKRSLNS

Sequences:

>Translated_476_residues
MSKEFDVVVIGAGPGGYIAAIRAAQLGKTVACIEKWKNPAGALKLGGTCLNVGCIPSKALLASSEEFENTSHHLADHGIT
VDGVKIDVAKMLGRKDAIVEKMTSGIEFLFKKNKITWLKGHGKFTGKTDAGVQIEVSGEGETEVVTAKNVIIATGSKARH
LPGIPVDNKIVSDNEGALTFDAVPKKLAVIGAGVIGLELGSVWRRLGAEVTVLEALPAFLGAADEALAKEAAKLFKKQGL
DINLGVQIGEVKATANGVSIAYTDKDGNAQTLDADRLIVSVGRVPNTDNLGLEAIGLKANERGFIDVDDHCRTAVPNVYA
IGDVVRGPMLAHKAEDEGVLVAEVIDGQKPHIDYNCIPWVIYTYPEIAWVGKTEQQLKAEGREIKSGKFPFSINGRALGM
NAPDGFVKMIADAKTDELLGVHVIAANASDLIAEAVVAMEFKAASEDIARICHPHPSMSEVMREAALAVDKRSLNS
>Mature_475_residues
SKEFDVVVIGAGPGGYIAAIRAAQLGKTVACIEKWKNPAGALKLGGTCLNVGCIPSKALLASSEEFENTSHHLADHGITV
DGVKIDVAKMLGRKDAIVEKMTSGIEFLFKKNKITWLKGHGKFTGKTDAGVQIEVSGEGETEVVTAKNVIIATGSKARHL
PGIPVDNKIVSDNEGALTFDAVPKKLAVIGAGVIGLELGSVWRRLGAEVTVLEALPAFLGAADEALAKEAAKLFKKQGLD
INLGVQIGEVKATANGVSIAYTDKDGNAQTLDADRLIVSVGRVPNTDNLGLEAIGLKANERGFIDVDDHCRTAVPNVYAI
GDVVRGPMLAHKAEDEGVLVAEVIDGQKPHIDYNCIPWVIYTYPEIAWVGKTEQQLKAEGREIKSGKFPFSINGRALGMN
APDGFVKMIADAKTDELLGVHVIAANASDLIAEAVVAMEFKAASEDIARICHPHPSMSEVMREAALAVDKRSLNS

Specific function: The branched-chain alpha-keto dehydrogenase complex catalyzes the overall conversion of alpha-keto acids to acyl-CoA and CO(2). It contains multiple copies of 3 enzymatic components:branched-chain alpha-keto acid decarboxylase (E1), lipoamide acyltransfer

COG id: COG1249

COG function: function code C; Pyruvate/2-oxoglutarate dehydrogenase complex, dihydrolipoamide dehydrogenase (E3) component, and related enzymes

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-I pyridine nucleotide-disulfide oxidoreductase family [H]

Homologues:

Organism=Homo sapiens, GI91199540, Length=469, Percent_Identity=47.7611940298507, Blast_Score=428, Evalue=1e-120,
Organism=Homo sapiens, GI50301238, Length=470, Percent_Identity=28.7234042553192, Blast_Score=172, Evalue=4e-43,
Organism=Homo sapiens, GI33519430, Length=468, Percent_Identity=25.4273504273504, Blast_Score=127, Evalue=2e-29,
Organism=Homo sapiens, GI33519428, Length=468, Percent_Identity=25.4273504273504, Blast_Score=127, Evalue=2e-29,
Organism=Homo sapiens, GI33519426, Length=468, Percent_Identity=25.4273504273504, Blast_Score=127, Evalue=2e-29,
Organism=Homo sapiens, GI148277065, Length=468, Percent_Identity=25.4273504273504, Blast_Score=127, Evalue=2e-29,
Organism=Homo sapiens, GI148277071, Length=468, Percent_Identity=25.4273504273504, Blast_Score=127, Evalue=3e-29,
Organism=Homo sapiens, GI22035672, Length=474, Percent_Identity=27.6371308016878, Blast_Score=127, Evalue=3e-29,
Organism=Homo sapiens, GI291045266, Length=479, Percent_Identity=24.6346555323591, Blast_Score=114, Evalue=2e-25,
Organism=Homo sapiens, GI291045268, Length=474, Percent_Identity=23.4177215189873, Blast_Score=100, Evalue=5e-21,
Organism=Escherichia coli, GI1786307, Length=468, Percent_Identity=38.6752136752137, Blast_Score=318, Evalue=4e-88,
Organism=Escherichia coli, GI87082354, Length=475, Percent_Identity=30.9473684210526, Blast_Score=209, Evalue=3e-55,
Organism=Escherichia coli, GI1789915, Length=465, Percent_Identity=30.1075268817204, Blast_Score=185, Evalue=4e-48,
Organism=Escherichia coli, GI87081717, Length=474, Percent_Identity=28.6919831223629, Blast_Score=165, Evalue=8e-42,
Organism=Caenorhabditis elegans, GI32565766, Length=471, Percent_Identity=48.619957537155, Blast_Score=440, Evalue=1e-124,
Organism=Caenorhabditis elegans, GI17557007, Length=494, Percent_Identity=28.3400809716599, Blast_Score=143, Evalue=2e-34,
Organism=Caenorhabditis elegans, GI71982272, Length=489, Percent_Identity=27.4028629856851, Blast_Score=130, Evalue=2e-30,
Organism=Caenorhabditis elegans, GI71983429, Length=468, Percent_Identity=27.7777777777778, Blast_Score=119, Evalue=3e-27,
Organism=Caenorhabditis elegans, GI71983419, Length=468, Percent_Identity=27.7777777777778, Blast_Score=119, Evalue=3e-27,
Organism=Caenorhabditis elegans, GI17559934, Length=193, Percent_Identity=30.5699481865285, Blast_Score=74, Evalue=1e-13,
Organism=Saccharomyces cerevisiae, GI6321091, Length=482, Percent_Identity=46.0580912863071, Blast_Score=411, Evalue=1e-115,
Organism=Saccharomyces cerevisiae, GI6325240, Length=477, Percent_Identity=30.398322851153, Blast_Score=220, Evalue=5e-58,
Organism=Saccharomyces cerevisiae, GI6325166, Length=474, Percent_Identity=26.5822784810127, Blast_Score=151, Evalue=2e-37,
Organism=Drosophila melanogaster, GI21358499, Length=470, Percent_Identity=49.7872340425532, Blast_Score=454, Evalue=1e-128,
Organism=Drosophila melanogaster, GI24640553, Length=490, Percent_Identity=27.9591836734694, Blast_Score=130, Evalue=2e-30,
Organism=Drosophila melanogaster, GI24640549, Length=490, Percent_Identity=27.9591836734694, Blast_Score=130, Evalue=2e-30,
Organism=Drosophila melanogaster, GI24640551, Length=490, Percent_Identity=27.9591836734694, Blast_Score=130, Evalue=3e-30,
Organism=Drosophila melanogaster, GI17737741, Length=489, Percent_Identity=26.3803680981595, Blast_Score=122, Evalue=6e-28,

Paralogues:

None

Copy number: 380 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 160 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 1880 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). 6,000 Molecules/Cell In: Glucose minimal

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR016156
- InterPro:   IPR013027
- InterPro:   IPR006258
- InterPro:   IPR004099
- InterPro:   IPR012999
- InterPro:   IPR001327 [H]

Pfam domain/function: PF00070 Pyr_redox; PF07992 Pyr_redox_2; PF02852 Pyr_redox_dim [H]

EC number: =1.8.1.4 [H]

Molecular weight: Translated: 50395; Mature: 50264

Theoretical pI: Translated: 6.00; Mature: 6.00

Prosite motif: PS00076 PYRIDINE_REDOX_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSKEFDVVVIGAGPGGYIAAIRAAQLGKTVACIEKWKNPAGALKLGGTCLNVGCIPSKAL
CCCCCCEEEEECCCCCHHHHHHHHHHCCHHHHHHHHCCCCCCEEECCEEEEECCCCCHHH
LASSEEFENTSHHLADHGITVDGVKIDVAKMLGRKDAIVEKMTSGIEFLFKKNKITWLKG
HCCCHHHCCCHHHHHHCCEEEECEEEEHHHHCCCHHHHHHHHHHHHHEEEECCCEEEEEC
HGKFTGKTDAGVQIEVSGEGETEVVTAKNVIIATGSKARHLPGIPVDNKIVSDNEGALTF
CCEECCCCCCCEEEEECCCCCEEEEEECEEEEECCCCCCCCCCCCCCCCEECCCCCCEEE
DAVPKKLAVIGAGVIGLELGSVWRRLGAEVTVLEALPAFLGAADEALAKEAAKLFKKQGL
CCCCCEEHHHCCCHHHHHHHHHHHHHCCCEEEHHHHHHHHCCHHHHHHHHHHHHHHHCCC
DINLGVQIGEVKATANGVSIAYTDKDGNAQTLDADRLIVSVGRVPNTDNLGLEAIGLKAN
EEEECEEEEEEEEECCCEEEEEECCCCCCEEECCCEEEEEECCCCCCCCCCEEEEEEECC
ERGFIDVDDHCRTAVPNVYAIGDVVRGPMLAHKAEDEGVLVAEVIDGQKPHIDYNCIPWV
CCCCEECCCCHHHCCCCEEEEHHHHCCCHHEECCCCCCEEEEEECCCCCCCCCCCCCCEE
IYTYPEIAWVGKTEQQLKAEGREIKSGKFPFSINGRALGMNAPDGFVKMIADAKTDELLG
EEECCCEEEECCCHHHHHHCCCCCCCCCCCEEECCEEEECCCCHHHHHHHHCCCCCCEEE
VHVIAANASDLIAEAVVAMEFKAASEDIARICHPHPSMSEVMREAALAVDKRSLNS
EEEEECCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHCCCC
>Mature Secondary Structure 
SKEFDVVVIGAGPGGYIAAIRAAQLGKTVACIEKWKNPAGALKLGGTCLNVGCIPSKAL
CCCCCEEEEECCCCCHHHHHHHHHHCCHHHHHHHHCCCCCCEEECCEEEEECCCCCHHH
LASSEEFENTSHHLADHGITVDGVKIDVAKMLGRKDAIVEKMTSGIEFLFKKNKITWLKG
HCCCHHHCCCHHHHHHCCEEEECEEEEHHHHCCCHHHHHHHHHHHHHEEEECCCEEEEEC
HGKFTGKTDAGVQIEVSGEGETEVVTAKNVIIATGSKARHLPGIPVDNKIVSDNEGALTF
CCEECCCCCCCEEEEECCCCCEEEEEECEEEEECCCCCCCCCCCCCCCCEECCCCCCEEE
DAVPKKLAVIGAGVIGLELGSVWRRLGAEVTVLEALPAFLGAADEALAKEAAKLFKKQGL
CCCCCEEHHHCCCHHHHHHHHHHHHHCCCEEEHHHHHHHHCCHHHHHHHHHHHHHHHCCC
DINLGVQIGEVKATANGVSIAYTDKDGNAQTLDADRLIVSVGRVPNTDNLGLEAIGLKAN
EEEECEEEEEEEEECCCEEEEEECCCCCCEEECCCEEEEEECCCCCCCCCCEEEEEEECC
ERGFIDVDDHCRTAVPNVYAIGDVVRGPMLAHKAEDEGVLVAEVIDGQKPHIDYNCIPWV
CCCCEECCCCHHHCCCCEEEEHHHHCCCHHEECCCCCCEEEEEECCCCCCCCCCCCCCEE
IYTYPEIAWVGKTEQQLKAEGREIKSGKFPFSINGRALGMNAPDGFVKMIADAKTDELLG
EEECCCEEEECCCHHHHHHCCCCCCCCCCCEEECCEEEECCCCHHHHHHHHCCCCCCEEE
VHVIAANASDLIAEAVVAMEFKAASEDIARICHPHPSMSEVMREAALAVDKRSLNS
EEEEECCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8867378 [H]