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The map label for this gene is emrB [H]

Identifier: 78066114

GI number: 78066114

Start: 1628115

End: 1629674

Strand: Reverse

Name: emrB [H]

Synonym: Bcep18194_A4644

Alternate gene names: 78066114

Gene position: 1629674-1628115 (Counterclockwise)

Preceding gene: 78066115

Following gene: 78066105

Centisome position: 44.12

GC content: 65.32

Gene sequence:

>1560_bases
ATGGCACAGGCTCCTGTCTCTCACCCGCCCTTGCAGGGCGGGCAACTCGTGATCGGGACGATCGCGGTATCGCTCGCGGT
GTTCATGAACGTGCTCGACACGTCCATCGCGAACGTCGCGATCCCGACCATCTCGGGCGACCTCGGCGTGTCGTCCGACC
AGGGCACGTGGGTCATCACGTCGTTCGCTGTCGCGAACGCAATCTCGGTGCCGCTGACCGGCTGGCTGACTGACCGCTTC
GGGCAGGTGCGCCTGTTCCTCGCGTCGATCATCCTCTTCGTGATTTCGTCGTGGATGTGCGGGCTCTCGCCCAACCTGCC
GTTCCTGCTTGGGTCACGCGTGCTGCAAGGCGCGGTGGCGGGCCCGATGATTCCGCTGTCGCAAGCGCTCCTGCTGTCGA
GCTATCCGCGCGCCAAAGCGCCCATGGCGCTCGCGCTATGGGCAATGACGACACTGATTGCCCCCGTTGCCGGCCCGATT
CTCGGCGGCTGGATCTCGGACAACTACTCGTGGCCGTGGATCTTCTACGTCAACATCCCGGTCGGCATCGCCGCCGCCGC
CGCGACGTGGGCGATCTACCGCAACCGGGAGTCGGCCGTGCGCCGCGCGCCGATCGACGGCGTAGGCCTCGCGCTGCTGG
TCATCTGGGTCGGCTCGCTGCAGATCATGCTCGACAAGGGCAAGGATCTCGACTGGTTCGCGTCGACGACCATCATCGTG
CTCGCGCTGACCGCCGTCATTGCGTTCGCGTTCTTTGTCATCTGGGAATTGACCGCCGAGCATCCGGTTGTCGACCTGTC
GCTGTTCCGCATGCGGAACTTCACCGGCGGCACGGTTGCGCTGTCGATCGGGTATGGCCTGTACTTCGGCAACCTCGTGC
TGTTGCCGCTGTGGCTGCAGACGCAGATCGGCTACACCGCGACCGACGCCGGTCTCGTGATGGCGCCGGTCGGGCTGTTC
GCCATCCTGCTGTCGCCGCTCACCGGGAAGTATCTGCCGCGCACCGATCCGCGCTTTATCTCGACGGCGTCGTTCCTGAC
GTTTGCGCTGTGCTTCTGGATGCGCTCGCGCTACACGACGGGCGTCGACGAATGGTCGCTGACGCTGCCGACGCTCGTGC
AGGGCATCGCGATGGCCGGCTTCTTCATCCCGCTGGTGTCGATCACGCTGTCCGGCCTGCCCGGCCACCGCATCCCCGCG
GCATCGGGCCTGTCGAACTTCGTGCGGATCATGTGCGGCGGTATCGGTACGTCGATCTTCCAGACGGCCTGGGACCATCG
CAACAACTTCCATCACGCGCAACTGGTCGAGCAGACGAACGTCTACAACCCGACGTTCAACCAGGCCGTCACGCAGATGG
GCAACCTCGGGCTGACGCAGGACCAGGCGCACGGGCTGATCAACAACATGGCCACGCAGCAGGCCGCGCAGCTCGGCGTG
AACGATCTGTTCTACATCTCGGCGGCGATCTTCGTGCTGCTGATCGGGCTGATCTGGATCACAAAGCCCGAGCGCTCCGG
TGGTGGCGATGCAGGTGCCGCGGCATCGGCTGCGCACTGA

Upstream 100 bases:

>100_bases
GCCGAGAACGCAGGCAGCAACGCATCGGCGCCGGCCGCGTCGAAGTCGAATGCCGTCGCGAAGATGATGTAACCGTTCCG
ACTCCGGAAAAACAACCGAC

Downstream 100 bases:

>100_bases
ACACGCAACCTGAACGCAAACAAAAAAGCCCGGTCGACATCGACCGGGCTTTTTTCATTTCGACGGAAGCGCGTTCGCGC
GGCTGTGTCAGGCCGCCGTA

Product: EmrB/QacA family drug resistance transporter

Products: Proton [Cytoplasm]; multidrug [Periplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 519; Mature: 518

Protein sequence:

>519_residues
MAQAPVSHPPLQGGQLVIGTIAVSLAVFMNVLDTSIANVAIPTISGDLGVSSDQGTWVITSFAVANAISVPLTGWLTDRF
GQVRLFLASIILFVISSWMCGLSPNLPFLLGSRVLQGAVAGPMIPLSQALLLSSYPRAKAPMALALWAMTTLIAPVAGPI
LGGWISDNYSWPWIFYVNIPVGIAAAAATWAIYRNRESAVRRAPIDGVGLALLVIWVGSLQIMLDKGKDLDWFASTTIIV
LALTAVIAFAFFVIWELTAEHPVVDLSLFRMRNFTGGTVALSIGYGLYFGNLVLLPLWLQTQIGYTATDAGLVMAPVGLF
AILLSPLTGKYLPRTDPRFISTASFLTFALCFWMRSRYTTGVDEWSLTLPTLVQGIAMAGFFIPLVSITLSGLPGHRIPA
ASGLSNFVRIMCGGIGTSIFQTAWDHRNNFHHAQLVEQTNVYNPTFNQAVTQMGNLGLTQDQAHGLINNMATQQAAQLGV
NDLFYISAAIFVLLIGLIWITKPERSGGGDAGAAASAAH

Sequences:

>Translated_519_residues
MAQAPVSHPPLQGGQLVIGTIAVSLAVFMNVLDTSIANVAIPTISGDLGVSSDQGTWVITSFAVANAISVPLTGWLTDRF
GQVRLFLASIILFVISSWMCGLSPNLPFLLGSRVLQGAVAGPMIPLSQALLLSSYPRAKAPMALALWAMTTLIAPVAGPI
LGGWISDNYSWPWIFYVNIPVGIAAAAATWAIYRNRESAVRRAPIDGVGLALLVIWVGSLQIMLDKGKDLDWFASTTIIV
LALTAVIAFAFFVIWELTAEHPVVDLSLFRMRNFTGGTVALSIGYGLYFGNLVLLPLWLQTQIGYTATDAGLVMAPVGLF
AILLSPLTGKYLPRTDPRFISTASFLTFALCFWMRSRYTTGVDEWSLTLPTLVQGIAMAGFFIPLVSITLSGLPGHRIPA
ASGLSNFVRIMCGGIGTSIFQTAWDHRNNFHHAQLVEQTNVYNPTFNQAVTQMGNLGLTQDQAHGLINNMATQQAAQLGV
NDLFYISAAIFVLLIGLIWITKPERSGGGDAGAAASAAH
>Mature_518_residues
AQAPVSHPPLQGGQLVIGTIAVSLAVFMNVLDTSIANVAIPTISGDLGVSSDQGTWVITSFAVANAISVPLTGWLTDRFG
QVRLFLASIILFVISSWMCGLSPNLPFLLGSRVLQGAVAGPMIPLSQALLLSSYPRAKAPMALALWAMTTLIAPVAGPIL
GGWISDNYSWPWIFYVNIPVGIAAAAATWAIYRNRESAVRRAPIDGVGLALLVIWVGSLQIMLDKGKDLDWFASTTIIVL
ALTAVIAFAFFVIWELTAEHPVVDLSLFRMRNFTGGTVALSIGYGLYFGNLVLLPLWLQTQIGYTATDAGLVMAPVGLFA
ILLSPLTGKYLPRTDPRFISTASFLTFALCFWMRSRYTTGVDEWSLTLPTLVQGIAMAGFFIPLVSITLSGLPGHRIPAA
SGLSNFVRIMCGGIGTSIFQTAWDHRNNFHHAQLVEQTNVYNPTFNQAVTQMGNLGLTQDQAHGLINNMATQQAAQLGVN
DLFYISAAIFVLLIGLIWITKPERSGGGDAGAAASAAH

Specific function: Translocase that confers resistance to substances of high hydrophobicity [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the major facilitator superfamily. EmrB family [H]

Homologues:

Organism=Escherichia coli, GI1789042, Length=498, Percent_Identity=55.2208835341365, Blast_Score=538, Evalue=1e-154,
Organism=Escherichia coli, GI1788710, Length=510, Percent_Identity=48.2352941176471, Blast_Score=466, Evalue=1e-132,
Organism=Escherichia coli, GI1790195, Length=374, Percent_Identity=33.1550802139037, Blast_Score=169, Evalue=5e-43,
Organism=Escherichia coli, GI1788392, Length=402, Percent_Identity=30.0995024875622, Blast_Score=136, Evalue=3e-33,
Organism=Escherichia coli, GI87081983, Length=420, Percent_Identity=23.5714285714286, Blast_Score=120, Evalue=2e-28,
Organism=Saccharomyces cerevisiae, GI6322958, Length=188, Percent_Identity=22.8723404255319, Blast_Score=70, Evalue=1e-12,
Organism=Saccharomyces cerevisiae, GI6321663, Length=150, Percent_Identity=28, Blast_Score=67, Evalue=6e-12,
Organism=Saccharomyces cerevisiae, GI6325455, Length=126, Percent_Identity=29.3650793650794, Blast_Score=66, Evalue=1e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004638
- InterPro:   IPR020846
- InterPro:   IPR011701
- InterPro:   IPR016196 [H]

Pfam domain/function: PF07690 MFS_1 [H]

EC number: NA

Molecular weight: Translated: 55538; Mature: 55406

Theoretical pI: Translated: 7.66; Mature: 7.66

Prosite motif: PS50850 MFS

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAQAPVSHPPLQGGQLVIGTIAVSLAVFMNVLDTSIANVAIPTISGDLGVSSDQGTWVIT
CCCCCCCCCCCCCCCEEHHHHHHHHHHHHHHHHHHHHHEEEEEECCCCCCCCCCCCEEEE
SFAVANAISVPLTGWLTDRFGQVRLFLASIILFVISSWMCGLSPNLPFLLGSRVLQGAVA
HHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHC
GPMIPLSQALLLSSYPRAKAPMALALWAMTTLIAPVAGPILGGWISDNYSWPWIFYVNIP
CCCHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCEEEEEECC
VGIAAAAATWAIYRNRESAVRRAPIDGVGLALLVIWVGSLQIMLDKGKDLDWFASTTIIV
HHHHHHHHHHHHHHCHHHHHHHCCCCHHHHHHHHHHHHCCEEEEECCCCCCHHHHHHHHH
LALTAVIAFAFFVIWELTAEHPVVDLSLFRMRNFTGGTVALSIGYGLYFGNLVLLPLWLQ
HHHHHHHHHHHHHHHHHHCCCCEEHHHHHHHHCCCCCEEEEEECHHHHHHHHHHHHHHHH
TQIGYTATDAGLVMAPVGLFAILLSPLTGKYLPRTDPRFISTASFLTFALCFWMRSRYTT
HHCCCEECCCCHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCC
GVDEWSLTLPTLVQGIAMAGFFIPLVSITLSGLPGHRIPAASGLSNFVRIMCGGIGTSIF
CCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHH
QTAWDHRNNFHHAQLVEQTNVYNPTFNQAVTQMGNLGLTQDQAHGLINNMATQQAAQLGV
HHHHHHCCCCCHHHHHHHCCCCCCCHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHCCC
NDLFYISAAIFVLLIGLIWITKPERSGGGDAGAAASAAH
HHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCC
>Mature Secondary Structure 
AQAPVSHPPLQGGQLVIGTIAVSLAVFMNVLDTSIANVAIPTISGDLGVSSDQGTWVIT
CCCCCCCCCCCCCCEEHHHHHHHHHHHHHHHHHHHHHEEEEEECCCCCCCCCCCCEEEE
SFAVANAISVPLTGWLTDRFGQVRLFLASIILFVISSWMCGLSPNLPFLLGSRVLQGAVA
HHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHC
GPMIPLSQALLLSSYPRAKAPMALALWAMTTLIAPVAGPILGGWISDNYSWPWIFYVNIP
CCCHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCEEEEEECC
VGIAAAAATWAIYRNRESAVRRAPIDGVGLALLVIWVGSLQIMLDKGKDLDWFASTTIIV
HHHHHHHHHHHHHHCHHHHHHHCCCCHHHHHHHHHHHHCCEEEEECCCCCCHHHHHHHHH
LALTAVIAFAFFVIWELTAEHPVVDLSLFRMRNFTGGTVALSIGYGLYFGNLVLLPLWLQ
HHHHHHHHHHHHHHHHHHCCCCEEHHHHHHHHCCCCCEEEEEECHHHHHHHHHHHHHHHH
TQIGYTATDAGLVMAPVGLFAILLSPLTGKYLPRTDPRFISTASFLTFALCFWMRSRYTT
HHCCCEECCCCHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCC
GVDEWSLTLPTLVQGIAMAGFFIPLVSITLSGLPGHRIPAASGLSNFVRIMCGGIGTSIF
CCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHH
QTAWDHRNNFHHAQLVEQTNVYNPTFNQAVTQMGNLGLTQDQAHGLINNMATQQAAQLGV
HHHHHHCCCCCHHHHHHHCCCCCCCHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHCCC
NDLFYISAAIFVLLIGLIWITKPERSGGGDAGAAASAAH
HHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Proton [Periplasm]; multidrug [Cytoplasm] [C]

Specific reaction: Proton [Periplasm] + multidrug [Cytoplasm] = Proton [Cytoplasm] + multidrug [Periplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]