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The map label for this gene is truB

Identifier: 78066113

GI number: 78066113

Start: 1627059

End: 1628027

Strand: Direct

Name: truB

Synonym: Bcep18194_A4643

Alternate gene names: 78066113

Gene position: 1627059-1628027 (Clockwise)

Preceding gene: 78066112

Following gene: 78066118

Centisome position: 44.04

GC content: 67.49

Gene sequence:

>969_bases
ATGCGGCCGCCAAGGACGACTGAGCTCGACCGGCCTATGACGACTGCAGCATCCCAACGTCCGCGCGTGCCCCGGCGCCT
GCTGGACGGTGTCCTCCTGCTCGACAAGCCGGTCGGCCTGTCGAGCAACGACGCGCTGATTCGCGCGAAGCGTCTGCTGC
TCGCGAAGAAAGCCGGTCACACCGGCACGCTCGATCCGCTGGCTTCGGGCCTGCTGCCGCTGTGCTTCGGCGAGGCGACG
AAGTTCTCGCAGGATCTGCTCGAAGCGGACAAGACCTACGAAGCGACGATGCGTCTCGGCCAGCGTACGGCCACCGGCGA
CGCGGAAGGCGAGGTGATCGACACGCGGCCGGTCGAATGCGACCGCGCAGCGGTGGAAGCCGCGCTCGTGCGCTTCACCG
GCGAGATCGTGCAGGTGCCACCGATGTATTCGGCGCTCAAGCGCGATGGCAAGCCGCTGTACGAATATGCGCGCGCGGGG
CAAACCGTCGAGCGCGAGGGCCGCAACGTGACGATCCTCGCTCTCGCGTTGCTCGCATGCGACTTGCCTGACGTGACGTT
TCGCGTGACCTGCAGCAAGGGCACGTATGTGCGCACGCTGGCGGAGGATATCGGCGAGGCACTCGGTTGCGGTGCGCACC
TGACGATGCTGCGTCGCACGGGTGTTGGTGCGCTGACGCTCGAGCATGCGGTGACGCTCGATGCGCTGTCCGATGCCGAC
GACGCGTCGCGCGATGCGTGGCTCCAGCCGGTCGACGCGCTGCTGTCGACATTTCCGCTGGTTCGTCTCGATGAAACCAG
CGCGAAGCGGTTCCTGCACGGCCAGCGTCTGCCGCTGTCGGCGCTCGACCCGATCGATGCGGCCGAAGGCGAGCGTGTGC
GTGTATATGACGCGACTCGGCTGCTCGGCGTGGCTCGCAAGGCGAACGGCGTACTGGCGCCGGAACGGCTCGTCGTTACG
GCGGCCTGA

Upstream 100 bases:

>100_bases
CTGCATTTCCACTACGACCAGACGATCGAGAAGGCCGTGGAAATGTCGCGCCTGATCAAGGAAGCGAACTCGACGCGCGC
GAAGGACGACGACGAGGCCG

Downstream 100 bases:

>100_bases
CACAGCCGCGCGAACGCGCTTCCGTCGAAATGAAAAAAGCCCGGTCGATGTCGACCGGGCTTTTTTGTTTGCGTTCAGGT
TGCGTGTTCAGTGCGCAGCC

Product: tRNA pseudouridine synthase B

Products: pseudouridine 5'-phosphate; H2O

Alternate protein names: tRNA pseudouridine 55 synthase; Psi55 synthase; tRNA pseudouridylate synthase; tRNA-uridine isomerase

Number of amino acids: Translated: 322; Mature: 322

Protein sequence:

>322_residues
MRPPRTTELDRPMTTAASQRPRVPRRLLDGVLLLDKPVGLSSNDALIRAKRLLLAKKAGHTGTLDPLASGLLPLCFGEAT
KFSQDLLEADKTYEATMRLGQRTATGDAEGEVIDTRPVECDRAAVEAALVRFTGEIVQVPPMYSALKRDGKPLYEYARAG
QTVEREGRNVTILALALLACDLPDVTFRVTCSKGTYVRTLAEDIGEALGCGAHLTMLRRTGVGALTLEHAVTLDALSDAD
DASRDAWLQPVDALLSTFPLVRLDETSAKRFLHGQRLPLSALDPIDAAEGERVRVYDATRLLGVARKANGVLAPERLVVT
AA

Sequences:

>Translated_322_residues
MRPPRTTELDRPMTTAASQRPRVPRRLLDGVLLLDKPVGLSSNDALIRAKRLLLAKKAGHTGTLDPLASGLLPLCFGEAT
KFSQDLLEADKTYEATMRLGQRTATGDAEGEVIDTRPVECDRAAVEAALVRFTGEIVQVPPMYSALKRDGKPLYEYARAG
QTVEREGRNVTILALALLACDLPDVTFRVTCSKGTYVRTLAEDIGEALGCGAHLTMLRRTGVGALTLEHAVTLDALSDAD
DASRDAWLQPVDALLSTFPLVRLDETSAKRFLHGQRLPLSALDPIDAAEGERVRVYDATRLLGVARKANGVLAPERLVVT
AA
>Mature_322_residues
MRPPRTTELDRPMTTAASQRPRVPRRLLDGVLLLDKPVGLSSNDALIRAKRLLLAKKAGHTGTLDPLASGLLPLCFGEAT
KFSQDLLEADKTYEATMRLGQRTATGDAEGEVIDTRPVECDRAAVEAALVRFTGEIVQVPPMYSALKRDGKPLYEYARAG
QTVEREGRNVTILALALLACDLPDVTFRVTCSKGTYVRTLAEDIGEALGCGAHLTMLRRTGVGALTLEHAVTLDALSDAD
DASRDAWLQPVDALLSTFPLVRLDETSAKRFLHGQRLPLSALDPIDAAEGERVRVYDATRLLGVARKANGVLAPERLVVT
AA

Specific function: Responsible for synthesis of pseudouridine from uracil- 55 in the psi GC loop of transfer RNAs

COG id: COG0130

COG function: function code J; Pseudouridine synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the pseudouridine synthase truB family. Type 1 subfamily

Homologues:

Organism=Homo sapiens, GI21040257, Length=251, Percent_Identity=36.6533864541833, Blast_Score=132, Evalue=4e-31,
Organism=Homo sapiens, GI215599015, Length=298, Percent_Identity=29.1946308724832, Blast_Score=82, Evalue=8e-16,
Organism=Homo sapiens, GI4503337, Length=298, Percent_Identity=29.1946308724832, Blast_Score=82, Evalue=9e-16,
Organism=Escherichia coli, GI2367200, Length=316, Percent_Identity=45.253164556962, Blast_Score=253, Evalue=1e-68,
Organism=Caenorhabditis elegans, GI17553978, Length=271, Percent_Identity=28.0442804428044, Blast_Score=96, Evalue=3e-20,
Organism=Saccharomyces cerevisiae, GI6323204, Length=285, Percent_Identity=29.4736842105263, Blast_Score=100, Evalue=3e-22,
Organism=Saccharomyces cerevisiae, GI6324037, Length=199, Percent_Identity=33.1658291457286, Blast_Score=98, Evalue=1e-21,
Organism=Drosophila melanogaster, GI281364189, Length=261, Percent_Identity=27.9693486590038, Blast_Score=86, Evalue=3e-17,
Organism=Drosophila melanogaster, GI281364187, Length=261, Percent_Identity=27.9693486590038, Blast_Score=86, Evalue=3e-17,
Organism=Drosophila melanogaster, GI281364185, Length=261, Percent_Identity=27.9693486590038, Blast_Score=86, Evalue=3e-17,
Organism=Drosophila melanogaster, GI281364183, Length=261, Percent_Identity=27.9693486590038, Blast_Score=86, Evalue=3e-17,
Organism=Drosophila melanogaster, GI62471759, Length=261, Percent_Identity=27.9693486590038, Blast_Score=86, Evalue=3e-17,
Organism=Drosophila melanogaster, GI17975520, Length=261, Percent_Identity=27.9693486590038, Blast_Score=86, Evalue=3e-17,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): TRUB_BURS3 (Q39H28)

Other databases:

- EMBL:   CP000151
- RefSeq:   YP_368882.1
- ProteinModelPortal:   Q39H28
- SMR:   Q39H28
- STRING:   Q39H28
- GeneID:   3749846
- GenomeReviews:   CP000151_GR
- KEGG:   bur:Bcep18194_A4643
- NMPDR:   fig|269483.3.peg.5962
- eggNOG:   COG0130
- HOGENOM:   HBG397258
- OMA:   LGCGAYV
- ProtClustDB:   PRK01851
- BioCyc:   BSP36773:BCEP18194_A4643-MONOMER
- HAMAP:   MF_01080
- InterPro:   IPR002501
- InterPro:   IPR020103
- InterPro:   IPR015947
- InterPro:   IPR014780
- InterPro:   IPR015240
- TIGRFAMs:   TIGR00431

Pfam domain/function: PF09157 TruB-C_2; PF01509 TruB_N; SSF55120 PsdUridine_synth_cat_dom; SSF88697 PUA-like

EC number: 4.2.1.70

Molecular weight: Translated: 34803; Mature: 34803

Theoretical pI: Translated: 6.92; Mature: 6.92

Prosite motif: NA

Important sites: ACT_SITE 65-65

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRPPRTTELDRPMTTAASQRPRVPRRLLDGVLLLDKPVGLSSNDALIRAKRLLLAKKAGH
CCCCCCCCCCCCHHHHHCCCCCCHHHHHCCEEEEECCCCCCCCHHHHHHHHHHHHHCCCC
TGTLDPLASGLLPLCFGEATKFSQDLLEADKTYEATMRLGQRTATGDAEGEVIDTRPVEC
CCCHHHHHHCCHHHHHCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCEEECCCCCH
DRAAVEAALVRFTGEIVQVPPMYSALKRDGKPLYEYARAGQTVEREGRNVTILALALLAC
HHHHHHHHHHHHCCCEEECCCHHHHHHHCCCHHHHHHHCCCCHHHCCCCCHHHHHHHHHC
DLPDVTFRVTCSKGTYVRTLAEDIGEALGCGAHLTMLRRTGVGALTLEHAVTLDALSDAD
CCCCCEEEEEECCCCHHHHHHHHHHHHHCCCHHHHHHHHCCCCEEEHHHHHHHHHHCCCC
DASRDAWLQPVDALLSTFPLVRLDETSAKRFLHGQRLPLSALDPIDAAEGERVRVYDATR
CCCHHHHHHHHHHHHHHCCCEEECCHHHHHHHCCCCCCCCCCCCCCCCCCCEEEEEHHHH
LLGVARKANGVLAPERLVVTAA
HHHHHHHCCCCCCCCEEEEEEC
>Mature Secondary Structure
MRPPRTTELDRPMTTAASQRPRVPRRLLDGVLLLDKPVGLSSNDALIRAKRLLLAKKAGH
CCCCCCCCCCCCHHHHHCCCCCCHHHHHCCEEEEECCCCCCCCHHHHHHHHHHHHHCCCC
TGTLDPLASGLLPLCFGEATKFSQDLLEADKTYEATMRLGQRTATGDAEGEVIDTRPVEC
CCCHHHHHHCCHHHHHCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCEEECCCCCH
DRAAVEAALVRFTGEIVQVPPMYSALKRDGKPLYEYARAGQTVEREGRNVTILALALLAC
HHHHHHHHHHHHCCCEEECCCHHHHHHHCCCHHHHHHHCCCCHHHCCCCCHHHHHHHHHC
DLPDVTFRVTCSKGTYVRTLAEDIGEALGCGAHLTMLRRTGVGALTLEHAVTLDALSDAD
CCCCCEEEEEECCCCHHHHHHHHHHHHHCCCHHHHHHHHCCCCEEEHHHHHHHHHHCCCC
DASRDAWLQPVDALLSTFPLVRLDETSAKRFLHGQRLPLSALDPIDAAEGERVRVYDATR
CCCHHHHHHHHHHHHHHCCCEEECCHHHHHHHCCCCCCCCCCCCCCCCCCCEEEEEHHHH
LLGVARKANGVLAPERLVVTAA
HHHHHHHCCCCCCCCEEEEEEC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: uracil; D-ribose 5-phosphate

Specific reaction: uracil + D-ribose 5-phosphate = pseudouridine 5'-phosphate + H2O

General reaction: addition of H2O; elimination of H2O; C-O bond cleavage [C]

Inhibitor: 1-(Tetrahydro-2-furanyl)-5-fluorouracil; 5-fluorouracil [C]

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA