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The map label for this gene is rstA [H]

Identifier: 78065965

GI number: 78065965

Start: 1460635

End: 1461360

Strand: Reverse

Name: rstA [H]

Synonym: Bcep18194_A4494

Alternate gene names: 78065965

Gene position: 1461360-1460635 (Counterclockwise)

Preceding gene: 78065969

Following gene: 78065964

Centisome position: 39.56

GC content: 68.32

Gene sequence:

>726_bases
ATGAACGAAGATCCACTCAAATACCGTGTACTGCTGATCGAGGACGACGACCGCCTGGCGCAGCTCGTCCGCGAATACCT
CGACGGCTATGAATTCGCGGTGACGGTCGTGCGCCGCGGCGACCTCGCCGTCGCGGCGGTGCGCGAGCACCAGCCGGCCC
TCGTGATACTCGACCTGATGCTGCCGAACCTCGACGGGATGGAAGTCTGCCGGCGCATCCGCGCGTTCACCAACGTCCCC
GTGCTGATCCTGACCGCGCGCCTGGACGTCTACGACCAGGTCGCGGGCCTCGAGACGGGCGCCGACGACTACGTGACGAA
GCCGATCGAGCCGCGCGTGCTGGTCGCCCGCGCCCGTGCGCTGCTGCGCCGTGCGCAGCCGGCCGTCGCGGAAGCGCCCG
TGGCCACGCCCGAAGCGCTCGTGTTCGGCGAACTGGCGATCTCGCCGCCGAACCGCACCGTCACGTGGCGCGGCGAGCCG
GTCGACCTGAAGACCGCCGAATTCAACCTGCTGCTGATCCTCGCGCGCGCGGCCGGCACCGTGCTGAGCCGCGACGACAT
CCTGAAGCAGTTGCGCGGGATCGAGTTCGACGGACTCGACCGCTCGGTCGATTCCGGCATCTCGAAGCTGCGCCGCCGCT
TCGAGGATGCGTCGTCGGAGCCGCACAAGATCAAGACGATCTGGGGCCGCGGCTACCTGTTCAGCCCTTCTGCGTGGGAC
GAATAA

Upstream 100 bases:

>100_bases
TCTCGCTCCCCCGGCCTTCGGCGCGCCGGATAGAATTCGCGTGCCGCGCCGCGCACCGGCTTGCCGGGGCGTGAGTTGGC
GCGTGCGCCCCGAGGAGACC

Downstream 100 bases:

>100_bases
ATGCTCCGCCCGTTGATCCGGCTGTATCTCGTCGTCATCCTGTTCGTCGCCGCGTCGATCATCTTCATCCAGCTGTCGTT
CGACCGGATCTTCTACGATC

Product: two component transcriptional regulator

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 241; Mature: 241

Protein sequence:

>241_residues
MNEDPLKYRVLLIEDDDRLAQLVREYLDGYEFAVTVVRRGDLAVAAVREHQPALVILDLMLPNLDGMEVCRRIRAFTNVP
VLILTARLDVYDQVAGLETGADDYVTKPIEPRVLVARARALLRRAQPAVAEAPVATPEALVFGELAISPPNRTVTWRGEP
VDLKTAEFNLLLILARAAGTVLSRDDILKQLRGIEFDGLDRSVDSGISKLRRRFEDASSEPHKIKTIWGRGYLFSPSAWD
E

Sequences:

>Translated_241_residues
MNEDPLKYRVLLIEDDDRLAQLVREYLDGYEFAVTVVRRGDLAVAAVREHQPALVILDLMLPNLDGMEVCRRIRAFTNVP
VLILTARLDVYDQVAGLETGADDYVTKPIEPRVLVARARALLRRAQPAVAEAPVATPEALVFGELAISPPNRTVTWRGEP
VDLKTAEFNLLLILARAAGTVLSRDDILKQLRGIEFDGLDRSVDSGISKLRRRFEDASSEPHKIKTIWGRGYLFSPSAWD
E
>Mature_241_residues
MNEDPLKYRVLLIEDDDRLAQLVREYLDGYEFAVTVVRRGDLAVAAVREHQPALVILDLMLPNLDGMEVCRRIRAFTNVP
VLILTARLDVYDQVAGLETGADDYVTKPIEPRVLVARARALLRRAQPAVAEAPVATPEALVFGELAISPPNRTVTWRGEP
VDLKTAEFNLLLILARAAGTVLSRDDILKQLRGIEFDGLDRSVDSGISKLRRRFEDASSEPHKIKTIWGRGYLFSPSAWD
E

Specific function: Member of the two-component regulatory system rstB/rstA [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasmic [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 response regulatory domain [H]

Homologues:

Organism=Escherichia coli, GI145693140, Length=236, Percent_Identity=42.7966101694915, Blast_Score=181, Evalue=5e-47,
Organism=Escherichia coli, GI1788394, Length=232, Percent_Identity=37.9310344827586, Blast_Score=167, Evalue=5e-43,
Organism=Escherichia coli, GI1789809, Length=233, Percent_Identity=37.3390557939914, Blast_Score=164, Evalue=3e-42,
Organism=Escherichia coli, GI1790860, Length=224, Percent_Identity=36.1607142857143, Blast_Score=128, Evalue=3e-31,
Organism=Escherichia coli, GI2367329, Length=236, Percent_Identity=34.7457627118644, Blast_Score=126, Evalue=1e-30,
Organism=Escherichia coli, GI1786599, Length=230, Percent_Identity=33.9130434782609, Blast_Score=124, Evalue=5e-30,
Organism=Escherichia coli, GI1790863, Length=234, Percent_Identity=31.6239316239316, Blast_Score=116, Evalue=1e-27,
Organism=Escherichia coli, GI87082012, Length=226, Percent_Identity=34.5132743362832, Blast_Score=116, Evalue=1e-27,
Organism=Escherichia coli, GI1786784, Length=227, Percent_Identity=32.15859030837, Blast_Score=110, Evalue=6e-26,
Organism=Escherichia coli, GI1786911, Length=228, Percent_Identity=34.2105263157895, Blast_Score=108, Evalue=3e-25,
Organism=Escherichia coli, GI1789402, Length=225, Percent_Identity=35.5555555555556, Blast_Score=104, Evalue=6e-24,
Organism=Escherichia coli, GI1787229, Length=231, Percent_Identity=29.004329004329, Blast_Score=103, Evalue=8e-24,
Organism=Escherichia coli, GI1787375, Length=227, Percent_Identity=30.8370044052863, Blast_Score=96, Evalue=2e-21,
Organism=Escherichia coli, GI1790552, Length=229, Percent_Identity=32.7510917030568, Blast_Score=95, Evalue=4e-21,
Organism=Escherichia coli, GI1790437, Length=141, Percent_Identity=32.6241134751773, Blast_Score=67, Evalue=1e-12,
Organism=Saccharomyces cerevisiae, GI6322000, Length=103, Percent_Identity=33.0097087378641, Blast_Score=67, Evalue=2e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011006
- InterPro:   IPR001867
- InterPro:   IPR016032
- InterPro:   IPR001789
- InterPro:   IPR011991 [H]

Pfam domain/function: PF00072 Response_reg; PF00486 Trans_reg_C [H]

EC number: NA

Molecular weight: Translated: 26971; Mature: 26971

Theoretical pI: Translated: 5.05; Mature: 5.05

Prosite motif: PS50110 RESPONSE_REGULATORY

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
1.2 %Met     (Translated Protein)
1.7 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
1.2 %Met     (Mature Protein)
1.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNEDPLKYRVLLIEDDDRLAQLVREYLDGYEFAVTVVRRGDLAVAAVREHQPALVILDLM
CCCCCEEEEEEEEECCHHHHHHHHHHHCCCEEEEEEEECCCEEEEECCCCCCEEEEEEEC
LPNLDGMEVCRRIRAFTNVPVLILTARLDVYDQVAGLETGADDYVTKPIEPRVLVARARA
CCCCCHHHHHHHHHHHCCCCEEEEEEHHHHHHHHHCCCCCCCCCCCCCCCCHHHHHHHHH
LLRRAQPAVAEAPVATPEALVFGELAISPPNRTVTWRGEPVDLKTAEFNLLLILARAAGT
HHHHCCCCHHCCCCCCCCCEEEEEEEECCCCCEEEECCCCCEEEECCCEEEEEEEHHHCC
VLSRDDILKQLRGIEFDGLDRSVDSGISKLRRRFEDASSEPHKIKTIWGRGYLFSPSAWD
CCCHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHCCCCCCEEEEEECCCEEECCCCCC
E
C
>Mature Secondary Structure
MNEDPLKYRVLLIEDDDRLAQLVREYLDGYEFAVTVVRRGDLAVAAVREHQPALVILDLM
CCCCCEEEEEEEEECCHHHHHHHHHHHCCCEEEEEEEECCCEEEEECCCCCCEEEEEEEC
LPNLDGMEVCRRIRAFTNVPVLILTARLDVYDQVAGLETGADDYVTKPIEPRVLVARARA
CCCCCHHHHHHHHHHHCCCCEEEEEEHHHHHHHHHCCCCCCCCCCCCCCCCHHHHHHHHH
LLRRAQPAVAEAPVATPEALVFGELAISPPNRTVTWRGEPVDLKTAEFNLLLILARAAGT
HHHHCCCCHHCCCCCCCCCEEEEEEEECCCCCEEEECCCCCEEEECCCEEEEEEEHHHCC
VLSRDDILKQLRGIEFDGLDRSVDSGISKLRRRFEDASSEPHKIKTIWGRGYLFSPSAWD
CCCHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHCCCCCCEEEEEECCCEEECCCCCC
E
C

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9097039; 9278503; 1495392 [H]