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The map label for this gene is yafC [C]

Identifier: 78065958

GI number: 78065958

Start: 1452005

End: 1452928

Strand: Direct

Name: yafC [C]

Synonym: Bcep18194_A4487

Alternate gene names: 78065958

Gene position: 1452005-1452928 (Clockwise)

Preceding gene: 78065952

Following gene: 78065959

Centisome position: 39.31

GC content: 66.45

Gene sequence:

>924_bases
ATGGACAAGTGGACGCAGATTGAATTTTTCGTGCAGGTCGCCGAACTGGGCAGCCTGTCGAAGGCCGCCGAGCGGCTCGG
GATGTCGAGCGCGGCGGCGAGCCGCTGCCTGAACGCGCTGGAGGAGCGGCTGGGTGCGCGGCTCGTCGAGCGCACCACGC
GGCGCCTGTGGCTGACCGACGCCGGGCATGAATATCACCGGCGCTGCGTCGCGATCCTGATGGAACTGTCGGAAGCCGAT
GCGGCGGTCAACGAAACGAGCGTCAATCCGGCCGGCACGCTGCGCGTGACCGCGTCGGTCTCGTTCGCGTCGATCTATAT
CGCACCGGCGTTGCCCGAGTTCCACCGGCGCTACCCGAACCTGAACGTACAGATCGTCGCGGCGAACCGCTATCCGGATT
TCATCGAGGCCGGCATCGACGTGGCGATCCGCACGCGCGAGCATGAAGCGGATTCGGGCATCACCGTGCGCAAGCTCGCC
GAGACGCGGCGCGTGCTGGCCGCGTCGCCCGGGTATCTGGCGAAGCACGGGGCGCCCGCGACGCCCGATGCGCTGTGCGA
CCACCGGCTGCTGGTCTACAACCTCGCGAACGATCCGTACGTGCTGCATTTCCGCCACGGCGCGAAGAAGCAGTCGGTCA
CGATCAAGAGCGTGCTCGATGCGAACGAAGGGCAGGTGATCCGCGCCGCGGCACTGGCCGGGCTCGGCATCCTGATCCAG
CCGCTCTACATCATTCACGACGATATCGTCGCGGGGAAGCTGGTGCCGGTGCTCACCGACTGGCAACTGCCGGCGCTCAC
CGTCAATATCGCGTACCAGAGCCGCCGTCACCAGCCGGCGAAGATCCGCGTGTTCACCGAGTTCCTGATCGAGCGTTTCG
AACGCCTCGGGCTCGCACGCAAATGGAAGGAAGTCGGCCGCTGA

Upstream 100 bases:

>100_bases
TCGTTGGGTGGCGCGTTGACGTAACATTAATTCCTCCTTGAATCGTAAAAAAGATGGTTTGGAGGAGTTTTGTTCTCCGC
CAAGTGGAAGAATCGAACGC

Downstream 100 bases:

>100_bases
GCGCAGGCCGGCGCTGGGCCGTCGCGCATTTCTCCGTGTGACGGAGAACTGTTCTCCCGAACGTCGTCTTTTTCGCCGCT
GTCGTGCGCTTTACATTGCG

Product: LysR family transcriptional regulator

Products: NA

Alternate protein names: ORF2 [H]

Number of amino acids: Translated: 307; Mature: 307

Protein sequence:

>307_residues
MDKWTQIEFFVQVAELGSLSKAAERLGMSSAAASRCLNALEERLGARLVERTTRRLWLTDAGHEYHRRCVAILMELSEAD
AAVNETSVNPAGTLRVTASVSFASIYIAPALPEFHRRYPNLNVQIVAANRYPDFIEAGIDVAIRTREHEADSGITVRKLA
ETRRVLAASPGYLAKHGAPATPDALCDHRLLVYNLANDPYVLHFRHGAKKQSVTIKSVLDANEGQVIRAAALAGLGILIQ
PLYIIHDDIVAGKLVPVLTDWQLPALTVNIAYQSRRHQPAKIRVFTEFLIERFERLGLARKWKEVGR

Sequences:

>Translated_307_residues
MDKWTQIEFFVQVAELGSLSKAAERLGMSSAAASRCLNALEERLGARLVERTTRRLWLTDAGHEYHRRCVAILMELSEAD
AAVNETSVNPAGTLRVTASVSFASIYIAPALPEFHRRYPNLNVQIVAANRYPDFIEAGIDVAIRTREHEADSGITVRKLA
ETRRVLAASPGYLAKHGAPATPDALCDHRLLVYNLANDPYVLHFRHGAKKQSVTIKSVLDANEGQVIRAAALAGLGILIQ
PLYIIHDDIVAGKLVPVLTDWQLPALTVNIAYQSRRHQPAKIRVFTEFLIERFERLGLARKWKEVGR
>Mature_307_residues
MDKWTQIEFFVQVAELGSLSKAAERLGMSSAAASRCLNALEERLGARLVERTTRRLWLTDAGHEYHRRCVAILMELSEAD
AAVNETSVNPAGTLRVTASVSFASIYIAPALPEFHRRYPNLNVQIVAANRYPDFIEAGIDVAIRTREHEADSGITVRKLA
ETRRVLAASPGYLAKHGAPATPDALCDHRLLVYNLANDPYVLHFRHGAKKQSVTIKSVLDANEGQVIRAAALAGLGILIQ
PLYIIHDDIVAGKLVPVLTDWQLPALTVNIAYQSRRHQPAKIRVFTEFLIERFERLGLARKWKEVGR

Specific function: Unknown

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH lysR-type DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1786401, Length=289, Percent_Identity=34.9480968858132, Blast_Score=163, Evalue=1e-41,
Organism=Escherichia coli, GI145693193, Length=291, Percent_Identity=31.9587628865979, Blast_Score=152, Evalue=2e-38,
Organism=Escherichia coli, GI1789440, Length=286, Percent_Identity=29.7202797202797, Blast_Score=131, Evalue=7e-32,
Organism=Escherichia coli, GI87081978, Length=290, Percent_Identity=31.0344827586207, Blast_Score=129, Evalue=2e-31,
Organism=Escherichia coli, GI1789639, Length=298, Percent_Identity=28.5234899328859, Blast_Score=125, Evalue=5e-30,
Organism=Escherichia coli, GI1787128, Length=283, Percent_Identity=28.2685512367491, Blast_Score=120, Evalue=1e-28,
Organism=Escherichia coli, GI1787589, Length=282, Percent_Identity=26.9503546099291, Blast_Score=103, Evalue=1e-23,
Organism=Escherichia coli, GI1789173, Length=275, Percent_Identity=26.5454545454545, Blast_Score=83, Evalue=2e-17,
Organism=Escherichia coli, GI157672245, Length=120, Percent_Identity=35, Blast_Score=80, Evalue=2e-16,
Organism=Escherichia coli, GI1788706, Length=267, Percent_Identity=25.4681647940075, Blast_Score=79, Evalue=4e-16,
Organism=Escherichia coli, GI1788748, Length=151, Percent_Identity=34.4370860927152, Blast_Score=76, Evalue=2e-15,
Organism=Escherichia coli, GI1787601, Length=176, Percent_Identity=28.9772727272727, Blast_Score=74, Evalue=9e-15,
Organism=Escherichia coli, GI1788481, Length=298, Percent_Identity=24.8322147651007, Blast_Score=67, Evalue=1e-12,
Organism=Escherichia coli, GI1787806, Length=243, Percent_Identity=27.1604938271605, Blast_Score=66, Evalue=3e-12,
Organism=Escherichia coli, GI1787879, Length=143, Percent_Identity=32.1678321678322, Blast_Score=64, Evalue=1e-11,

Paralogues:

None

Copy number: 10-20 Molecules/Cell [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000847
- InterPro:   IPR005119
- InterPro:   IPR011991 [H]

Pfam domain/function: PF00126 HTH_1; PF03466 LysR_substrate [H]

EC number: NA

Molecular weight: Translated: 34147; Mature: 34147

Theoretical pI: Translated: 9.51; Mature: 9.51

Prosite motif: PS50931 HTH_LYSR

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
1.0 %Met     (Translated Protein)
2.0 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
1.0 %Met     (Mature Protein)
2.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDKWTQIEFFVQVAELGSLSKAAERLGMSSAAASRCLNALEERLGARLVERTTRRLWLTD
CCCCHHHHHHHHHHHHCCHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHEEEEEC
AGHEYHRRCVAILMELSEADAAVNETSVNPAGTLRVTASVSFASIYIAPALPEFHRRYPN
CCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCEEEEEEEEEEEEEEEECCCHHHHHHCCC
LNVQIVAANRYPDFIEAGIDVAIRTREHEADSGITVRKLAETRRVLAASPGYLAKHGAPA
CCEEEEECCCCCHHHHCCCCEEEEECCCCCCCCCHHHHHHHHHHHHHCCCCCHHCCCCCC
TPDALCDHRLLVYNLANDPYVLHFRHGAKKQSVTIKSVLDANEGQVIRAAALAGLGILIQ
CHHHHHCCEEEEEEECCCCEEEEEECCCCCCCEEHHHHHCCCCCCEEHHHHHHHHHHHHH
PLYIIHDDIVAGKLVPVLTDWQLPALTVNIAYQSRRHQPAKIRVFTEFLIERFERLGLAR
HHHHHCCCHHHCCEEEEEECCCCCEEEEEEEECCCCCCCCEEHHHHHHHHHHHHHHCHHH
KWKEVGR
HHHHHCC
>Mature Secondary Structure
MDKWTQIEFFVQVAELGSLSKAAERLGMSSAAASRCLNALEERLGARLVERTTRRLWLTD
CCCCHHHHHHHHHHHHCCHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHEEEEEC
AGHEYHRRCVAILMELSEADAAVNETSVNPAGTLRVTASVSFASIYIAPALPEFHRRYPN
CCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCEEEEEEEEEEEEEEEECCCHHHHHHCCC
LNVQIVAANRYPDFIEAGIDVAIRTREHEADSGITVRKLAETRRVLAASPGYLAKHGAPA
CCEEEEECCCCCHHHHCCCCEEEEECCCCCCCCCHHHHHHHHHHHHHCCCCCHHCCCCCC
TPDALCDHRLLVYNLANDPYVLHFRHGAKKQSVTIKSVLDANEGQVIRAAALAGLGILIQ
CHHHHHCCEEEEEEECCCCEEEEEECCCCCCCEEHHHHHCCCCCCEEHHHHHHHHHHHHH
PLYIIHDDIVAGKLVPVLTDWQLPALTVNIAYQSRRHQPAKIRVFTEFLIERFERLGLAR
HHHHHCCCHHHCCEEEEEECCCCCEEEEEEEECCCCCCCCEEHHHHHHHHHHHHHHCHHH
KWKEVGR
HHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8635745; 7542800 [H]