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The map label for this gene is rnhA [H]

Identifier: 78065898

GI number: 78065898

Start: 1384168

End: 1384611

Strand: Reverse

Name: rnhA [H]

Synonym: Bcep18194_A4427

Alternate gene names: 78065898

Gene position: 1384611-1384168 (Counterclockwise)

Preceding gene: 78065899

Following gene: 78065897

Centisome position: 37.48

GC content: 65.09

Gene sequence:

>444_bases
ATGACTACCGATACCATCGACATCTATACCGACGGCGCCTGCAAGGGCAACCCCGGCCCCGGCGGCTGGGGCGCACTGAT
GCGCTACGGCGACAAAGAGAAGGAGCTGTTCGGCGGCGAGCCGAACACGACCAACAACCGCATGGAGCTGATGGGCGTGA
TCGCCGCGCTCGAAGCGCTGAAGCGGCCGTGCCAGGTGATCGTGCATACCGACTCGCAATACGTGCAGAAAGGCATCAGC
GAGTGGATCCACGGCTGGAAGAAGAAAGGCTGGATCACCGCGGCAAAGACGCCCGTGAAGAACGCCGACCTGTGGAAGCG
GCTCGACGCGCTCGTCGCGCAGCACGAGGTCGAGTGGCGCTGGGTCAAGGGCCACGCGGGCCATCCCGAAAACGAGCGCG
CGGACGCACTCGCGAATCGCGGCGTCGAATCGCTCACGGCCTGA

Upstream 100 bases:

>100_bases
TGCGCGGCATGCGCCTCGTCGGCCAGATCAAGATGAAGAAGCCCGTGCTCGCACCGGGCCTGACGCCGGCGGCCTCCCCG
ACCACCCATCAAGAACATTC

Downstream 100 bases:

>100_bases
ACGCGGGCCGCTCCCCTGTTTCTATTGTTTTTCCCGACATGCGCCAGATCATTCTCGATACCGAAACCACCGGCCTGAAC
GCCCGCACGGGCGACCGCCT

Product: ribonuclease H

Products: NA

Alternate protein names: RNase H [H]

Number of amino acids: Translated: 147; Mature: 146

Protein sequence:

>147_residues
MTTDTIDIYTDGACKGNPGPGGWGALMRYGDKEKELFGGEPNTTNNRMELMGVIAALEALKRPCQVIVHTDSQYVQKGIS
EWIHGWKKKGWITAAKTPVKNADLWKRLDALVAQHEVEWRWVKGHAGHPENERADALANRGVESLTA

Sequences:

>Translated_147_residues
MTTDTIDIYTDGACKGNPGPGGWGALMRYGDKEKELFGGEPNTTNNRMELMGVIAALEALKRPCQVIVHTDSQYVQKGIS
EWIHGWKKKGWITAAKTPVKNADLWKRLDALVAQHEVEWRWVKGHAGHPENERADALANRGVESLTA
>Mature_146_residues
TTDTIDIYTDGACKGNPGPGGWGALMRYGDKEKELFGGEPNTTNNRMELMGVIAALEALKRPCQVIVHTDSQYVQKGISE
WIHGWKKKGWITAAKTPVKNADLWKRLDALVAQHEVEWRWVKGHAGHPENERADALANRGVESLTA

Specific function: Endonuclease that specifically degrades the RNA of RNA- DNA hybrids [H]

COG id: COG0328

COG function: function code L; Ribonuclease HI

Gene ontology:

Cell location: Cytoplasm (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 RNase H domain [H]

Homologues:

Organism=Homo sapiens, GI21359816, Length=151, Percent_Identity=33.7748344370861, Blast_Score=74, Evalue=5e-14,
Organism=Escherichia coli, GI1786408, Length=133, Percent_Identity=65.4135338345865, Blast_Score=197, Evalue=3e-52,
Organism=Caenorhabditis elegans, GI115532542, Length=147, Percent_Identity=40.8163265306122, Blast_Score=96, Evalue=9e-21,
Organism=Caenorhabditis elegans, GI17505482, Length=131, Percent_Identity=32.824427480916, Blast_Score=64, Evalue=3e-11,
Organism=Drosophila melanogaster, GI17137476, Length=143, Percent_Identity=36.3636363636364, Blast_Score=82, Evalue=1e-16,
Organism=Drosophila melanogaster, GI45552509, Length=143, Percent_Identity=36.3636363636364, Blast_Score=82, Evalue=1e-16,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR022892
- InterPro:   IPR012337
- InterPro:   IPR002156 [H]

Pfam domain/function: PF00075 RnaseH [H]

EC number: =3.1.26.4 [H]

Molecular weight: Translated: 16309; Mature: 16177

Theoretical pI: Translated: 7.63; Mature: 7.63

Prosite motif: PS50879 RNASE_H

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
4.1 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTTDTIDIYTDGACKGNPGPGGWGALMRYGDKEKELFGGEPNTTNNRMELMGVIAALEAL
CCCCEEEEEECCCCCCCCCCCHHHHHHHCCCCHHHHCCCCCCCCCHHHHHHHHHHHHHHH
KRPCQVIVHTDSQYVQKGISEWIHGWKKKGWITAAKTPVKNADLWKRLDALVAQHEVEWR
HCCCEEEEECCHHHHHHHHHHHHHHHHCCCCEEECCCCCCCHHHHHHHHHHHHHHCCCEE
WVKGHAGHPENERADALANRGVESLTA
EEECCCCCCCCHHHHHHHHCCHHHHCC
>Mature Secondary Structure 
TTDTIDIYTDGACKGNPGPGGWGALMRYGDKEKELFGGEPNTTNNRMELMGVIAALEAL
CCCEEEEEECCCCCCCCCCCHHHHHHHCCCCHHHHCCCCCCCCCHHHHHHHHHHHHHHH
KRPCQVIVHTDSQYVQKGISEWIHGWKKKGWITAAKTPVKNADLWKRLDALVAQHEVEWR
HCCCEEEEECCHHHHHHHHHHHHHHHHCCCCEEECCCCCCCHHHHHHHHHHHHHHCCCEE
WVKGHAGHPENERADALANRGVESLTA
EEECCCCCCCCHHHHHHHHCCHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 14500782 [H]