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The map label for this gene is glcF

Identifier: 78065294

GI number: 78065294

Start: 718106

End: 719332

Strand: Reverse

Name: glcF

Synonym: Bcep18194_A3818

Alternate gene names: 78065294

Gene position: 719332-718106 (Counterclockwise)

Preceding gene: 78065295

Following gene: 78065293

Centisome position: 19.47

GC content: 68.46

Gene sequence:

>1227_bases
ATGCAAACGAACCTCGCCGATTTCATCCGCAATACGCCCGACGGCGACGAGGCCGACGCGATATTGCGCAAGTGCGTGCA
TTGCGGGTTTTGTACCGCGACCTGCCCGACCTACCAGTTGCTCGGCGACGAACTCGACGGCCCGCGCGGCCGTATCTACC
TGATCAAGCAGATGGTCGAAGGCGCGCCCGTCACGCGCAGCACGCAGCAGCACCTCGACCGCTGCCTCACGTGCCGCAGC
TGCGAGACGACCTGCCCGTCCGGCGTCCAGTACGGCCGGCTCGTGGAGATCGGCCGCAAGCATGTCGAGGCGCAGGTGCA
GCGCCCGGTGCAGCAGCGTCTCGTGCGCCGCCTGCTCGCGAGCCTCGTGCCGAACGAGGCGCTGTTCTCGCCGGTGATGC
GGCTCGGCCAGCACGTGCGGCCGCTGCTGCCGAAGCGGCTGCGCGACAAGGTGCCGCCGCGCACGCGGCTGCTCGAATGG
CCGCACCTCACCCATACGCGCAAGATGTTGATGCTCGGCGGCTGCGTGCAGCCTTCGATGCTGCCGAACATCAACATCGC
GACCGCGCGCGTGCTGGACGCGCTCGGCATCGAGACGGTCGTCTCGCCCGACGCGGGCTGCTGCGGCGCGATCCGCCTGC
ACCTGAACTATCACGACGAAGCGCTCGACGACGCGCGCCGCAACATCGACGCATGGTGGCCCCATGTCGAACAGGGCGTC
GAGGCGATCGTGATGAACGCGTCGGGCTGTGGCGCCACGGTGCTCGAATACGCGCACCTGCTGCGCGACGACCCGGCCTA
CGCGGAGAAGGCGCAGCGCATCGTGTCGCTCACGCGCGACATCTCCGACGTGCTGCTCGCGTTCGAGGCCGAACTCGCGA
CGCTCGCGCGGCGCCGTGCGATCCATACGGTCGCGTACCACCCGCCGTGCACGCTGCAACACGGCCAGCAGTCGCGCGGC
AAGGTCGAACGCCTGCTGGAGACGCTCGGCATCGACGTGCGGCTGCCGGCCGACAGCCATCTGTGCTGCGGGTCGGCCGG
CACCTATTCGCTGACGCAGCCGTCGCTGTCGTACCGGTTGCGCAAGCAGAAGCTGCTGAAGCTGCAGGCGCTCGAACCGC
AGATGATCGTCTCCGGCAACGTCGGCTGCATCGCGCACCTGCAGAGCGGCACGCAGATTCCGGTCGCCCACTGGATCCAG
CTCGTCGAGCATCTGCTGTACGGCTAG

Upstream 100 bases:

>100_bases
CCCGCCCCGATGATGAAAATCCACCGCGGGCTGAAGGCCGCGTTCGATCCCGCGCGCATCTTCAACCGCGGCCGGCTCTA
CCCCGATCTCTGAGGGCTCG

Downstream 100 bases:

>100_bases
GGCCGGTTCATGCGAATGACGGATGGGCGCCGGGCCTCGCGCGCGGCGTCCGTATAATGGGCGGATCGATGCGTCGCGTG
CCGCGGCGCATCCACACCAT

Product: glycolate oxidase iron-sulfur subunit

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 408; Mature: 408

Protein sequence:

>408_residues
MQTNLADFIRNTPDGDEADAILRKCVHCGFCTATCPTYQLLGDELDGPRGRIYLIKQMVEGAPVTRSTQQHLDRCLTCRS
CETTCPSGVQYGRLVEIGRKHVEAQVQRPVQQRLVRRLLASLVPNEALFSPVMRLGQHVRPLLPKRLRDKVPPRTRLLEW
PHLTHTRKMLMLGGCVQPSMLPNINIATARVLDALGIETVVSPDAGCCGAIRLHLNYHDEALDDARRNIDAWWPHVEQGV
EAIVMNASGCGATVLEYAHLLRDDPAYAEKAQRIVSLTRDISDVLLAFEAELATLARRRAIHTVAYHPPCTLQHGQQSRG
KVERLLETLGIDVRLPADSHLCCGSAGTYSLTQPSLSYRLRKQKLLKLQALEPQMIVSGNVGCIAHLQSGTQIPVAHWIQ
LVEHLLYG

Sequences:

>Translated_408_residues
MQTNLADFIRNTPDGDEADAILRKCVHCGFCTATCPTYQLLGDELDGPRGRIYLIKQMVEGAPVTRSTQQHLDRCLTCRS
CETTCPSGVQYGRLVEIGRKHVEAQVQRPVQQRLVRRLLASLVPNEALFSPVMRLGQHVRPLLPKRLRDKVPPRTRLLEW
PHLTHTRKMLMLGGCVQPSMLPNINIATARVLDALGIETVVSPDAGCCGAIRLHLNYHDEALDDARRNIDAWWPHVEQGV
EAIVMNASGCGATVLEYAHLLRDDPAYAEKAQRIVSLTRDISDVLLAFEAELATLARRRAIHTVAYHPPCTLQHGQQSRG
KVERLLETLGIDVRLPADSHLCCGSAGTYSLTQPSLSYRLRKQKLLKLQALEPQMIVSGNVGCIAHLQSGTQIPVAHWIQ
LVEHLLYG
>Mature_408_residues
MQTNLADFIRNTPDGDEADAILRKCVHCGFCTATCPTYQLLGDELDGPRGRIYLIKQMVEGAPVTRSTQQHLDRCLTCRS
CETTCPSGVQYGRLVEIGRKHVEAQVQRPVQQRLVRRLLASLVPNEALFSPVMRLGQHVRPLLPKRLRDKVPPRTRLLEW
PHLTHTRKMLMLGGCVQPSMLPNINIATARVLDALGIETVVSPDAGCCGAIRLHLNYHDEALDDARRNIDAWWPHVEQGV
EAIVMNASGCGATVLEYAHLLRDDPAYAEKAQRIVSLTRDISDVLLAFEAELATLARRRAIHTVAYHPPCTLQHGQQSRG
KVERLLETLGIDVRLPADSHLCCGSAGTYSLTQPSLSYRLRKQKLLKLQALEPQMIVSGNVGCIAHLQSGTQIPVAHWIQ
LVEHLLYG

Specific function: Unknown

COG id: COG0247

COG function: function code C; Fe-S oxidoreductase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 2 4Fe-4S ferredoxin-type domains [H]

Homologues:

Organism=Escherichia coli, GI48994913, Length=407, Percent_Identity=53.3169533169533, Blast_Score=436, Evalue=1e-123,
Organism=Escherichia coli, GI1786497, Length=236, Percent_Identity=22.0338983050847, Blast_Score=75, Evalue=8e-15,
Organism=Escherichia coli, GI1788576, Length=416, Percent_Identity=22.1153846153846, Blast_Score=74, Evalue=1e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR017896
- InterPro:   IPR017900
- InterPro:   IPR004017
- InterPro:   IPR012285
- InterPro:   IPR012257 [H]

Pfam domain/function: PF02754 CCG [H]

EC number: NA

Molecular weight: Translated: 45394; Mature: 45394

Theoretical pI: Translated: 8.26; Mature: 8.26

Prosite motif: PS00198 4FE4S_FERREDOXIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

3.9 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
5.9 %Cys+Met (Translated Protein)
3.9 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
5.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQTNLADFIRNTPDGDEADAILRKCVHCGFCTATCPTYQLLGDELDGPRGRIYLIKQMVE
CCCCHHHHHCCCCCCCHHHHHHHHHHHCCCCCCCCCHHHHHCCCCCCCCCHHHHHHHHHC
GAPVTRSTQQHLDRCLTCRSCETTCPSGVQYGRLVEIGRKHVEAQVQRPVQQRLVRRLLA
CCCCCCHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
SLVPNEALFSPVMRLGQHVRPLLPKRLRDKVPPRTRLLEWPHLTHTRKMLMLGGCVQPSM
HHCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHCCCCCCHHHHHHHHHHCCCCCCCC
LPNINIATARVLDALGIETVVSPDAGCCGAIRLHLNYHDEALDDARRNIDAWWPHVEQGV
CCCCCHHHHHHHHHHCCHHHCCCCCCCCEEEEEEECCCHHHHHHHHHCCHHCCHHHHCCC
EAIVMNASGCGATVLEYAHLLRDDPAYAEKAQRIVSLTRDISDVLLAFEAELATLARRRA
CEEEECCCCCCHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
IHTVAYHPPCTLQHGQQSRGKVERLLETLGIDVRLPADSHLCCGSAGTYSLTQPSLSYRL
HHHEEECCCCCCCCCCCHHHHHHHHHHHHCCCEECCCCCCEECCCCCCCCCCCCHHHHHH
RKQKLLKLQALEPQMIVSGNVGCIAHLQSGTQIPVAHWIQLVEHLLYG
HHHHHHHHHCCCCCEEEECCCCEEEEECCCCCCCHHHHHHHHHHHHCC
>Mature Secondary Structure
MQTNLADFIRNTPDGDEADAILRKCVHCGFCTATCPTYQLLGDELDGPRGRIYLIKQMVE
CCCCHHHHHCCCCCCCHHHHHHHHHHHCCCCCCCCCHHHHHCCCCCCCCCHHHHHHHHHC
GAPVTRSTQQHLDRCLTCRSCETTCPSGVQYGRLVEIGRKHVEAQVQRPVQQRLVRRLLA
CCCCCCHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
SLVPNEALFSPVMRLGQHVRPLLPKRLRDKVPPRTRLLEWPHLTHTRKMLMLGGCVQPSM
HHCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHCCCCCCHHHHHHHHHHCCCCCCCC
LPNINIATARVLDALGIETVVSPDAGCCGAIRLHLNYHDEALDDARRNIDAWWPHVEQGV
CCCCCHHHHHHHHHHCCHHHCCCCCCCCEEEEEEECCCHHHHHHHHHCCHHCCHHHHCCC
EAIVMNASGCGATVLEYAHLLRDDPAYAEKAQRIVSLTRDISDVLLAFEAELATLARRRA
CEEEECCCCCCHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
IHTVAYHPPCTLQHGQQSRGKVERLLETLGIDVRLPADSHLCCGSAGTYSLTQPSLSYRL
HHHEEECCCCCCCCCCCHHHHHHHHHHHHCCCEECCCCCCEECCCCCCCCCCCCHHHHHH
RKQKLLKLQALEPQMIVSGNVGCIAHLQSGTQIPVAHWIQLVEHLLYG
HHHHHHHHHCCCCCEEEECCCCEEEEECCCCCCCHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: Fe [C]

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8606183; 9278503 [H]