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The map label for this gene is murF [H]

Identifier: 78065117

GI number: 78065117

Start: 524717

End: 526123

Strand: Direct

Name: murF [H]

Synonym: Bcep18194_A3641

Alternate gene names: 78065117

Gene position: 524717-526123 (Clockwise)

Preceding gene: 78065116

Following gene: 78065118

Centisome position: 14.2

GC content: 70.72

Gene sequence:

>1407_bases
ATGACGATGCTCAGTCTCGGCGAAGCCGCTCGCCTGATCCCCGGCGCAACCGTCCACGGCGACGCCGGCGTCACGTTCGA
CCGCGTGTCGACGGACAGCCGCACGGCCGGCCCCGGCGACCTGTTCGTCGCACTGAAAGGCGAGCGCTTCGACGCGCACG
ACTTCCTCGGCGACGTCGCTGCGCGCGGTGCCTCCGCGGCGCTGGTCGCGCACGTGCCGGCGGGTGTCACGATGCCGGCG
ATCGAAGGCGGCGAAACGCGTGCCGCACTCGGCGCGCTTGCGCATGGCTGGCGCAAACGATTCGCGTTGCCGCTCGTTGC
GGTGACGGGCAGCAACGGCAAGACGACCGTCAAGGAAATGATCGCGTCGATCTTCGCGGCGGCGGTCGGCGCCGACGCGC
GGCTCGCGACGGCCGGCAACCTGAACAACGATGTCGGCCTGCCGCTGACGCTGCTGCGCCTGTCGGCCGCGCATCGTCTC
GCGGTGATCGAGATCGGGATGAACCACCCGGGCGAGACCGAAATCCTCGCGCGCCTCACGGCGCCGACGGTCGCGCTCGT
CAACAACGCACAGCGCGAGCACCAGGAATTCATGGCGACGGTCGAAGCCGTCGCGCTCGAACACGCGGCCGTGATACACG
CGCTGCCGCCGGACGGCGTCGCGGTGTTCCCGGCCGATGACGCATATGCGGGCATCTGGCGCGTCGCCGCAACCGGCAAC
CGGATCCTCGATTTCGCGCTGCACGATGCCGGACGGCAGAACGACGCGCAGGTCACGGGCCGTCTGCACGGCGGCGAACT
CGCGATCGACACGCCCGCGGGCGTCGTCACGGTGCGGCTGCGCGCGCTCGGCGAGCACAATGCGCGTAACGCGCTGGCCG
CCACGGCCGCGGCGCTGGGTGCCGGCGTTGCACTGTCGGCGATCAAGCAGGGCCTCGAATCGTTCGAGCCGGTCAAGGGC
CGGCTGCAGGTGAAGCAGGCGATCGCAGGCAGCCTCGCGGGTGCAACGGTCGTCGACGATACGTACAACGCGAATCCCGA
TTCGATGCGTGCCGCGATCGACGTGCTCGCCGCGCAACCGGCGCCGCGCGTGCTGGTGATCGGCGACATGGGCGAGGTCG
GCGACGAAGGGCCGGCTTTCCATCGCGAGGTCGGTGCCTACGCGCGCGAGCGCGGGATCGACGCGCTGTTCGCGCTCGGC
GATGCGTCGCGCGATGCCTGCACGGCATACGGCGACACGGCCCGCCATTTCGGCGACGTCGGCGCGCTGGTTGAAGCACT
GCTCGCAGCGGGCTACGGCGCGCAGGCGACGCTGCTCGTGAAGGGCTCGCGGTACATGAAGATGGAGCGCGTGGTCGACG
CGCTGACGAACCAACCCGCGGCGGGCACTGCGCCCGCTGCACACTGA

Upstream 100 bases:

>100_bases
CCACGCAGGAAATCATGGGCAAGAAGCGCACGTTCTCCGATCAGGATCACGCACGGCTCGCGCTGGCAGCGCGCGCGACG
CACGGCAAGGGAGGCGCCGA

Downstream 100 bases:

>100_bases
GTAGAAGGAAGGAAGCATGCTGCTGGCGCTGGCGCAATGGCTGCAAGGAGACGCAAGCTTTTTGCGCTTGTTCACGTACC
TCACGTTCCGTGCGGTGATG

Product: UDP-N-acetylmuramoyl-tripeptide--D-alanyl-D- alanine ligase

Products: NA

Alternate protein names: D-alanyl-D-alanine-adding enzyme; UDP-MurNAc-pentapeptide synthetase [H]

Number of amino acids: Translated: 468; Mature: 467

Protein sequence:

>468_residues
MTMLSLGEAARLIPGATVHGDAGVTFDRVSTDSRTAGPGDLFVALKGERFDAHDFLGDVAARGASAALVAHVPAGVTMPA
IEGGETRAALGALAHGWRKRFALPLVAVTGSNGKTTVKEMIASIFAAAVGADARLATAGNLNNDVGLPLTLLRLSAAHRL
AVIEIGMNHPGETEILARLTAPTVALVNNAQREHQEFMATVEAVALEHAAVIHALPPDGVAVFPADDAYAGIWRVAATGN
RILDFALHDAGRQNDAQVTGRLHGGELAIDTPAGVVTVRLRALGEHNARNALAATAAALGAGVALSAIKQGLESFEPVKG
RLQVKQAIAGSLAGATVVDDTYNANPDSMRAAIDVLAAQPAPRVLVIGDMGEVGDEGPAFHREVGAYARERGIDALFALG
DASRDACTAYGDTARHFGDVGALVEALLAAGYGAQATLLVKGSRYMKMERVVDALTNQPAAGTAPAAH

Sequences:

>Translated_468_residues
MTMLSLGEAARLIPGATVHGDAGVTFDRVSTDSRTAGPGDLFVALKGERFDAHDFLGDVAARGASAALVAHVPAGVTMPA
IEGGETRAALGALAHGWRKRFALPLVAVTGSNGKTTVKEMIASIFAAAVGADARLATAGNLNNDVGLPLTLLRLSAAHRL
AVIEIGMNHPGETEILARLTAPTVALVNNAQREHQEFMATVEAVALEHAAVIHALPPDGVAVFPADDAYAGIWRVAATGN
RILDFALHDAGRQNDAQVTGRLHGGELAIDTPAGVVTVRLRALGEHNARNALAATAAALGAGVALSAIKQGLESFEPVKG
RLQVKQAIAGSLAGATVVDDTYNANPDSMRAAIDVLAAQPAPRVLVIGDMGEVGDEGPAFHREVGAYARERGIDALFALG
DASRDACTAYGDTARHFGDVGALVEALLAAGYGAQATLLVKGSRYMKMERVVDALTNQPAAGTAPAAH
>Mature_467_residues
TMLSLGEAARLIPGATVHGDAGVTFDRVSTDSRTAGPGDLFVALKGERFDAHDFLGDVAARGASAALVAHVPAGVTMPAI
EGGETRAALGALAHGWRKRFALPLVAVTGSNGKTTVKEMIASIFAAAVGADARLATAGNLNNDVGLPLTLLRLSAAHRLA
VIEIGMNHPGETEILARLTAPTVALVNNAQREHQEFMATVEAVALEHAAVIHALPPDGVAVFPADDAYAGIWRVAATGNR
ILDFALHDAGRQNDAQVTGRLHGGELAIDTPAGVVTVRLRALGEHNARNALAATAAALGAGVALSAIKQGLESFEPVKGR
LQVKQAIAGSLAGATVVDDTYNANPDSMRAAIDVLAAQPAPRVLVIGDMGEVGDEGPAFHREVGAYARERGIDALFALGD
ASRDACTAYGDTARHFGDVGALVEALLAAGYGAQATLLVKGSRYMKMERVVDALTNQPAAGTAPAAH

Specific function: Involved in cell wall formation. Catalyzes the final step in the synthesis of UDP-N-acetylmuramoyl-pentapeptide, the precursor of murein [H]

COG id: COG0770

COG function: function code M; UDP-N-acetylmuramyl pentapeptide synthase

Gene ontology:

Cell location: Cytoplasm (Probable) [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the MurCDEF family [H]

Homologues:

Organism=Escherichia coli, GI1786274, Length=438, Percent_Identity=43.1506849315069, Blast_Score=270, Evalue=1e-73,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004101
- InterPro:   IPR013221
- InterPro:   IPR000713
- InterPro:   IPR005863 [H]

Pfam domain/function: PF01225 Mur_ligase; PF02875 Mur_ligase_C; PF08245 Mur_ligase_M [H]

EC number: =6.3.2.10 [H]

Molecular weight: Translated: 48030; Mature: 47899

Theoretical pI: Translated: 6.11; Mature: 6.11

Prosite motif: PS00211 ABC_TRANSPORTER_1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
2.4 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
2.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTMLSLGEAARLIPGATVHGDAGVTFDRVSTDSRTAGPGDLFVALKGERFDAHDFLGDVA
CCCCCCCCHHHHCCCCEEECCCCCEEEECCCCCCCCCCCEEEEEEECCCCCHHHHHHHHH
ARGASAALVAHVPAGVTMPAIEGGETRAALGALAHGWRKRFALPLVAVTGSNGKTTVKEM
HCCCCEEEEEECCCCCEECCCCCCCHHHHHHHHHHHHHHHHCCEEEEEECCCCCHHHHHH
IASIFAAAVGADARLATAGNLNNDVGLPLTLLRLSAAHRLAVIEIGMNHPGETEILARLT
HHHHHHHHHCCCCEEEECCCCCCCCCCHHHHHHHHHCCEEEEEEECCCCCCCHHHHHHHC
APTVALVNNAQREHQEFMATVEAVALEHAAVIHALPPDGVAVFPADDAYAGIWRVAATGN
CCHHHHHHCHHHHHHHHHHHHHHHHHHHHHEEEECCCCCEEEECCCCCCCCEEEEEECCC
RILDFALHDAGRQNDAQVTGRLHGGELAIDTPAGVVTVRLRALGEHNARNALAATAAALG
CEEEEHHHCCCCCCCCEEEEEECCCEEEEECCCCEEEEEEEHHCCCCCHHHHHHHHHHHH
AGVALSAIKQGLESFEPVKGRLQVKQAIAGSLAGATVVDDTYNANPDSMRAAIDVLAAQP
HHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHCCCEEECCCCCCCHHHHHHHHHHHHCCC
APRVLVIGDMGEVGDEGPAFHREVGAYARERGIDALFALGDASRDACTAYGDTARHFGDV
CCCEEEEECCCCCCCCCCHHHHHHHHHHHHCCCCEEEEECCCCCCHHHHCCCHHHHHHHH
GALVEALLAAGYGAQATLLVKGSRYMKMERVVDALTNQPAAGTAPAAH
HHHHHHHHHCCCCCCEEEEEECCHHHHHHHHHHHHHCCCCCCCCCCCC
>Mature Secondary Structure 
TMLSLGEAARLIPGATVHGDAGVTFDRVSTDSRTAGPGDLFVALKGERFDAHDFLGDVA
CCCCCCCHHHHCCCCEEECCCCCEEEECCCCCCCCCCCEEEEEEECCCCCHHHHHHHHH
ARGASAALVAHVPAGVTMPAIEGGETRAALGALAHGWRKRFALPLVAVTGSNGKTTVKEM
HCCCCEEEEEECCCCCEECCCCCCCHHHHHHHHHHHHHHHHCCEEEEEECCCCCHHHHHH
IASIFAAAVGADARLATAGNLNNDVGLPLTLLRLSAAHRLAVIEIGMNHPGETEILARLT
HHHHHHHHHCCCCEEEECCCCCCCCCCHHHHHHHHHCCEEEEEEECCCCCCCHHHHHHHC
APTVALVNNAQREHQEFMATVEAVALEHAAVIHALPPDGVAVFPADDAYAGIWRVAATGN
CCHHHHHHCHHHHHHHHHHHHHHHHHHHHHEEEECCCCCEEEECCCCCCCCEEEEEECCC
RILDFALHDAGRQNDAQVTGRLHGGELAIDTPAGVVTVRLRALGEHNARNALAATAAALG
CEEEEHHHCCCCCCCCEEEEEECCCEEEEECCCCEEEEEEEHHCCCCCHHHHHHHHHHHH
AGVALSAIKQGLESFEPVKGRLQVKQAIAGSLAGATVVDDTYNANPDSMRAAIDVLAAQP
HHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHCCCEEECCCCCCCHHHHHHHHHHHHCCC
APRVLVIGDMGEVGDEGPAFHREVGAYARERGIDALFALGDASRDACTAYGDTARHFGDV
CCCEEEEECCCCCCCCCCHHHHHHHHHHHHCCCCEEEEECCCCCCHHHHCCCHHHHHHHH
GALVEALLAAGYGAQATLLVKGSRYMKMERVVDALTNQPAAGTAPAAH
HHHHHHHHHCCCCCCEEEEEECCHHHHHHHHHHHHHCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 7542800 [H]