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The map label for this gene is murE

Identifier: 78065116

GI number: 78065116

Start: 523182

End: 524720

Strand: Direct

Name: murE

Synonym: Bcep18194_A3640

Alternate gene names: 78065116

Gene position: 523182-524720 (Clockwise)

Preceding gene: 78065115

Following gene: 78065117

Centisome position: 14.16

GC content: 70.24

Gene sequence:

>1539_bases
ATGAGCGCCGCTCGCAGTTCCCATCCGGCGCATCAGCAGATCGCAGCCGCGCTTGCGTGGCTGCGTCAGCATGTGGCCCC
CGCCGCGCAACTGCATGCCGACACGCGCAGCCTCAAGGCTGGCGACGTGTTCGTCGCGTACGCGGTCGACGGGGCAGACA
ATCGTGCCTTCATCGCCGACGCCGTCGCGCGCGGTGCGGCCGCCGTGCTGTATCAGCCGGAAGGGCTGGCCGCTGCGCCG
GCCGTTCCTGTCGCGCTTGCGGTGCCTGCGCTCGACCAGCTCGCCGGCGAGATCGCCAGCAGCTGGTATGGTGATCCGAG
CGACAGCCTGCTCGCGATCGGTGTGACCGGCACGAACGGCAAGACGTCGTGCACGCAATGGATCGCCGCCGCGTTGACGG
CGCTTCACCAGCCGTGCGCGGTGATCGGCACGCTCGGCACCGGGATGCCCGGCCAGCTCGTGCCGACCGGCTTCACGACC
CCCGATGCGTCGCAGTTGCAGCGCAGCCTTGCGCAGCTGCGCGATGCGGGCGCGAAGGCGGTCGCGATGGAAGTGTCGTC
GCACGCGCTGCACCAGGGGCGCGTGAACGGTACGGCGTTCGACATCGCGGTGTTTACGAATCTAACGCAGGACCACCTCG
ACTATCACGGCACGTTCGATGCATACGAGGCCGCGAAGGCGAAGCTGTTCGCATGGCGCGGCCTGCGCGCGGCGGTCATC
AACCGCGACGACGCAGCCGGACAGCGCCTGCTCGAGAAACTGGCCGGCCGCGTGCGCACGATCGCGTACGGAATCGGCGA
TGCATCGGCGCCGGACGCCGATCGCGAACTGGTCGCGCTCGACGTGCGCGCCACCACGACCGGCACGGCGTTCCGCCTGC
GTTCGTCCTGGGGCGACGCGGACGTCGAGGTCGGCACGCTCGGCACGTTCAACGTCAGCAACCTGCTCGCGGTGCTCGGC
TCGCTGCTCGCGGCCGACGTGCCGTTCGACGCGGCGCTCGCCGAGATCGCACGGCTCGAGTCCGTCAACGGCCGGATGCA
GCGGCTCGGCGGCCGGCTGCAGAACGACGAACCGCTCGTCGTGATCGACTACGCACACACGCCCGACGCGCTCGAAAAGA
CGCTCGACGCGCTGCGCCCGATCGCCACCGCGCGCGGCGGCCGGCTCGTCTGCATGTTCGGCTGCGGCGGCGATCGCGAT
GCGACGAAGCGCCCGCTGATGGGCGCGATCGCGGAGCGGTTCGCCGATGAAACCGTCGTCACGAGCGACAACCCGCGCAG
CGAGGATCCGCAGCGCATCATCGACCAGATCGTCGCGGGCATGACCGCGCCCGATCACGCACGCCGTATCGAGGATCGCG
CGAGCGCGATCCTGCAGGCCGTGCGCGGCGCCGCACGCGAGGATGTCGTCGTGCTGGCCGGCAAGGGCCACGAGGCCACG
CAGGAAATCATGGGCAAGAAGCGCACGTTCTCCGATCAGGATCACGCACGGCTCGCGCTGGCAGCGCGCGCGACGCACGG
CAAGGGAGGCGCCGAATGA

Upstream 100 bases:

>100_bases
CAGCACCTTCCGCAACCGGGCCGCAAAAGCTGGCCGCGGGTAGCGGTGCGAAGCATATGATCGTGTCCAGCACCACGCGT
AATTCACCAGGAGTTGTTCG

Downstream 100 bases:

>100_bases
CGATGCTCAGTCTCGGCGAAGCCGCTCGCCTGATCCCCGGCGCAACCGTCCACGGCGACGCCGGCGTCACGTTCGACCGC
GTGTCGACGGACAGCCGCAC

Product: UDP-N-acetylmuramoylalanyl-D-glutamate--2, 6-diaminopimelate ligase

Products: NA

Alternate protein names: Meso-A2pm-adding enzyme; Meso-diaminopimelate-adding enzyme; UDP-MurNAc-L-Ala-D-Glu:meso-diaminopimelate ligase; UDP-MurNAc-tripeptide synthetase; UDP-N-acetylmuramyl-tripeptide synthetase [H]

Number of amino acids: Translated: 512; Mature: 511

Protein sequence:

>512_residues
MSAARSSHPAHQQIAAALAWLRQHVAPAAQLHADTRSLKAGDVFVAYAVDGADNRAFIADAVARGAAAVLYQPEGLAAAP
AVPVALAVPALDQLAGEIASSWYGDPSDSLLAIGVTGTNGKTSCTQWIAAALTALHQPCAVIGTLGTGMPGQLVPTGFTT
PDASQLQRSLAQLRDAGAKAVAMEVSSHALHQGRVNGTAFDIAVFTNLTQDHLDYHGTFDAYEAAKAKLFAWRGLRAAVI
NRDDAAGQRLLEKLAGRVRTIAYGIGDASAPDADRELVALDVRATTTGTAFRLRSSWGDADVEVGTLGTFNVSNLLAVLG
SLLAADVPFDAALAEIARLESVNGRMQRLGGRLQNDEPLVVIDYAHTPDALEKTLDALRPIATARGGRLVCMFGCGGDRD
ATKRPLMGAIAERFADETVVTSDNPRSEDPQRIIDQIVAGMTAPDHARRIEDRASAILQAVRGAAREDVVVLAGKGHEAT
QEIMGKKRTFSDQDHARLALAARATHGKGGAE

Sequences:

>Translated_512_residues
MSAARSSHPAHQQIAAALAWLRQHVAPAAQLHADTRSLKAGDVFVAYAVDGADNRAFIADAVARGAAAVLYQPEGLAAAP
AVPVALAVPALDQLAGEIASSWYGDPSDSLLAIGVTGTNGKTSCTQWIAAALTALHQPCAVIGTLGTGMPGQLVPTGFTT
PDASQLQRSLAQLRDAGAKAVAMEVSSHALHQGRVNGTAFDIAVFTNLTQDHLDYHGTFDAYEAAKAKLFAWRGLRAAVI
NRDDAAGQRLLEKLAGRVRTIAYGIGDASAPDADRELVALDVRATTTGTAFRLRSSWGDADVEVGTLGTFNVSNLLAVLG
SLLAADVPFDAALAEIARLESVNGRMQRLGGRLQNDEPLVVIDYAHTPDALEKTLDALRPIATARGGRLVCMFGCGGDRD
ATKRPLMGAIAERFADETVVTSDNPRSEDPQRIIDQIVAGMTAPDHARRIEDRASAILQAVRGAAREDVVVLAGKGHEAT
QEIMGKKRTFSDQDHARLALAARATHGKGGAE
>Mature_511_residues
SAARSSHPAHQQIAAALAWLRQHVAPAAQLHADTRSLKAGDVFVAYAVDGADNRAFIADAVARGAAAVLYQPEGLAAAPA
VPVALAVPALDQLAGEIASSWYGDPSDSLLAIGVTGTNGKTSCTQWIAAALTALHQPCAVIGTLGTGMPGQLVPTGFTTP
DASQLQRSLAQLRDAGAKAVAMEVSSHALHQGRVNGTAFDIAVFTNLTQDHLDYHGTFDAYEAAKAKLFAWRGLRAAVIN
RDDAAGQRLLEKLAGRVRTIAYGIGDASAPDADRELVALDVRATTTGTAFRLRSSWGDADVEVGTLGTFNVSNLLAVLGS
LLAADVPFDAALAEIARLESVNGRMQRLGGRLQNDEPLVVIDYAHTPDALEKTLDALRPIATARGGRLVCMFGCGGDRDA
TKRPLMGAIAERFADETVVTSDNPRSEDPQRIIDQIVAGMTAPDHARRIEDRASAILQAVRGAAREDVVVLAGKGHEATQ
EIMGKKRTFSDQDHARLALAARATHGKGGAE

Specific function: Catalyzes the addition of meso-diaminopimelic acid to the nucleotide precursor UDP-N-acetylmuramoyl-L-alanyl-D-glutamate (UMAG) in the biosynthesis of bacterial cell-wall peptidoglycan [H]

COG id: COG0769

COG function: function code M; UDP-N-acetylmuramyl tripeptide synthase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the MurCDEF family. MurE subfamily [H]

Homologues:

Organism=Escherichia coli, GI1786273, Length=470, Percent_Identity=45.1063829787234, Blast_Score=322, Evalue=4e-89,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004101
- InterPro:   IPR013221
- InterPro:   IPR005761 [H]

Pfam domain/function: PF02875 Mur_ligase_C; PF08245 Mur_ligase_M [H]

EC number: =6.3.2.13 [H]

Molecular weight: Translated: 53710; Mature: 53579

Theoretical pI: Translated: 6.40; Mature: 6.40

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
2.3 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
2.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSAARSSHPAHQQIAAALAWLRQHVAPAAQLHADTRSLKAGDVFVAYAVDGADNRAFIAD
CCCCCCCCCHHHHHHHHHHHHHHHCCHHHHHHHHHHCCCCCCEEEEEEECCCCCCHHHHH
AVARGAAAVLYQPEGLAAAPAVPVALAVPALDQLAGEIASSWYGDPSDSLLAIGVTGTNG
HHHCCCEEEEECCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCEEEEEEECCCC
KTSCTQWIAAALTALHQPCAVIGTLGTGMPGQLVPTGFTTPDASQLQRSLAQLRDAGAKA
CHHHHHHHHHHHHHHHCCHHHHHHCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHCCCCE
VAMEVSSHALHQGRVNGTAFDIAVFTNLTQDHLDYHGTFDAYEAAKAKLFAWRGLRAAVI
EEEHHHHHHHHCCCCCCEEEEEHHHHCCCHHHHCCCCCCHHHHHHHHHHHHHCCCEEEEE
NRDDAAGQRLLEKLAGRVRTIAYGIGDASAPDADRELVALDVRATTTGTAFRLRSSWGDA
CCCHHHHHHHHHHHHHHHHEEEEECCCCCCCCCCCCEEEEEEEECCCCCEEEEECCCCCC
DVEVGTLGTFNVSNLLAVLGSLLAADVPFDAALAEIARLESVNGRMQRLGGRLQNDEPLV
CEEECCCCCCCHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHCHHHHHHCCCCCCCCCEE
VIDYAHTPDALEKTLDALRPIATARGGRLVCMFGCGGDRDATKRPLMGAIAERFADETVV
EEEECCCCHHHHHHHHHHHHHHHCCCCEEEEEEECCCCCCCCCCHHHHHHHHHHCCCCEE
TSDNPRSEDPQRIIDQIVAGMTAPDHARRIEDRASAILQAVRGAAREDVVVLAGKGHEAT
ECCCCCCCCHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHCCCCCCEEEEECCCCHHH
QEIMGKKRTFSDQDHARLALAARATHGKGGAE
HHHHCCCCCCCCCCHHEEEEEEECCCCCCCCC
>Mature Secondary Structure 
SAARSSHPAHQQIAAALAWLRQHVAPAAQLHADTRSLKAGDVFVAYAVDGADNRAFIAD
CCCCCCCCHHHHHHHHHHHHHHHCCHHHHHHHHHHCCCCCCEEEEEEECCCCCCHHHHH
AVARGAAAVLYQPEGLAAAPAVPVALAVPALDQLAGEIASSWYGDPSDSLLAIGVTGTNG
HHHCCCEEEEECCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCEEEEEEECCCC
KTSCTQWIAAALTALHQPCAVIGTLGTGMPGQLVPTGFTTPDASQLQRSLAQLRDAGAKA
CHHHHHHHHHHHHHHHCCHHHHHHCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHCCCCE
VAMEVSSHALHQGRVNGTAFDIAVFTNLTQDHLDYHGTFDAYEAAKAKLFAWRGLRAAVI
EEEHHHHHHHHCCCCCCEEEEEHHHHCCCHHHHCCCCCCHHHHHHHHHHHHHCCCEEEEE
NRDDAAGQRLLEKLAGRVRTIAYGIGDASAPDADRELVALDVRATTTGTAFRLRSSWGDA
CCCHHHHHHHHHHHHHHHHEEEEECCCCCCCCCCCCEEEEEEEECCCCCEEEEECCCCCC
DVEVGTLGTFNVSNLLAVLGSLLAADVPFDAALAEIARLESVNGRMQRLGGRLQNDEPLV
CEEECCCCCCCHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHCHHHHHHCCCCCCCCCEE
VIDYAHTPDALEKTLDALRPIATARGGRLVCMFGCGGDRDATKRPLMGAIAERFADETVV
EEEECCCCHHHHHHHHHHHHHHHCCCCEEEEEEECCCCCCCCCCHHHHHHHHHHCCCCEE
TSDNPRSEDPQRIIDQIVAGMTAPDHARRIEDRASAILQAVRGAAREDVVVLAGKGHEAT
ECCCCCCCCHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHCCCCCCEEEEECCCCHHH
QEIMGKKRTFSDQDHARLALAARATHGKGGAE
HHHHCCCCCCCCCCHHEEEEEEECCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11823852 [H]