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The map label for this gene is penA [H]

Identifier: 78065115

GI number: 78065115

Start: 521338

End: 523185

Strand: Direct

Name: penA [H]

Synonym: Bcep18194_A3639

Alternate gene names: 78065115

Gene position: 521338-523185 (Clockwise)

Preceding gene: 78065114

Following gene: 78065116

Centisome position: 14.11

GC content: 65.1

Gene sequence:

>1848_bases
ATGAAGCCGTCCCAGAAGCGCCAGAACGTGAAATTCTCGTCGAGCCCCGTGCTGGGCGTGCATCTGCCGATGTGGCGCTC
GAAGCTCGTCGTGTTCATGCTGTTCATGGCGTTCGTCGCGCTGGCCGCGCGCGCGTTCTGGATCCAGGGGCCCGGCAACG
CGTTCTATCGGAAGCAGGGCGAAAGCCGCTACCAGCGCACGCTCGATCTGCCGGCGACGCGCGGCAAGATCCTCGACCGT
AACGGGCTCGTGCTCGCGACGAGCCTGCCCGTGCGTGCGATCTGGGCGATTCCCGACGCGGTGCCGGACGATCTCGACGC
CGACAAGGTCAACCAGCTCGGCAAGCTCCTCGGCATGACGTCGAAGGAACTACGCGTGAAGCTGTCGGAAGACAAGGGTT
TCGTCTACGTCAAGCGCCAGGTGCCGATCGACGTCGCGGACAAAGTCGCTGCACTCGACATCCCTGGTATCTATCAGCGC
AACGAATACAAGCGCTTCTATCCGGAAGGCGAGATCACCGCTCACCTGATCGGCTTCACGAACGTCGAGGACGAAGGGCA
GGAGGGCGTCGAGCTGGGCGACCAGAAGATGCTGTCCGGCACGTCGGGCGTGCGTCGCGTGATCAAGGACCGCATGGGGC
ACATCGTCGAGGACGTCGCCGAGCAGATCCCGCCGCACAACGGCACGGATGTCGACCTGTCGATCGACAGCAAGATCCAG
TACATCGCGTACGCGAACCTGAAGGCCGCCGTCGAGAAGTTCAAGGCGAAGGCCGGCGCGGCGATGGTCGTCGACGTGCG
CACCGGCGAGGTGCTGGCACTCGTCAACTACCCGACGTACAACCCGAACGACCGCTCGCGCATGACGGGCGAGCAGTTGC
GCAACCGGATCATGACCGACGTGTTCGAGCCGGGCTCGATCATGAAGCCGTTTACGGTGTCGCTCGCACTCGACCTGCAT
CGCGTGACGCCGAACACGCTCGTCGAGACGGGCAACGGGCATTTCGTGCTCGACGGCGCACCGATTACCGACGACGCGGG
TTTCGGCACGCTGACGGTCGGCGGTGTGATCCAGAAGTCGAGCAACATCGGCGCGACGAAGATCGCGATGACGATGCGGC
CCGAGGAAATGTGGAATATGTATACGAGCATCGGCCTCGGCCAGGCGCCGAAGGTCGGATTCCCGGGCGCGGTGGCTGGC
CGCCTGCGTCCGTGGAAGAGCTGGCGCCGCATCGAACAGGCGACGATGTCGTACGGCTACGGCCTGTCGGTGTCGCTGTT
CCAGCTCGCGCGTGCCTACACGGCGATCGCGCATGACGGCGAGCTGATGCCCGTGACCATTTTCAAGACCGACCCCAACC
AGCCGGTCGTGGGCACGCAGGTGTTCAACCCGACCACCGCGCGCGAAGTGCGCGCGATGCTCGAGACGGTGGTCGCGCCG
GGTGGCACGTCGCCGGATGCGGCCGTGCCGGGTTATCGCGTCGGCGGCAAGAGCGGCACCGCGTACAAGCATGAAGGCCA
CGGCTACACGCGCAAGTACCGCGCGTCGTTCGTCGGGATGGCGCCGATGCCGAATCCGCGCATCGTCGTCGCGGTGTCGG
TCGACGAACCGACCGCCGGCAGCCACTTCGGCGGCCAGGTGTCCGGCCCCGTATTCTCGGCGATCGCCGGTGACACGATG
CGTGCGCTGAACGTCCCACCGAACATGCCGATCAAGCAGCTCGTCGTGTCTGACGATTCGCCGGCAGCACCTTCCGCAAC
CGGGCCGCAAAAGCTGGCCGCGGGTAGCGGTGCGAAGCATATGATCGTGTCCAGCACCACGCGTAATTCACCAGGAGTTG
TTCGATGA

Upstream 100 bases:

>100_bases
GCACGCAATACCTGACGCTGCCGCCGGGCGCTGCGAAGGCGATCGATGCGCCGATCCCGGCTTCGGCGGACACGGCCGCC
AAGGGTAAGGGAGGCGCGCG

Downstream 100 bases:

>100_bases
GCGCCGCTCGCAGTTCCCATCCGGCGCATCAGCAGATCGCAGCCGCGCTTGCGTGGCTGCGTCAGCATGTGGCCCCCGCC
GCGCAACTGCATGCCGACAC

Product: peptidoglycan synthetase FtsI

Products: [GlcNAc-(1->4)-Mur2Ac(oyl-L-Ala-gamma-D-Glu-L-Lys-D-Ala-D-Ala)]n+1-; $diphosphoundecaprenol; undecaprenyl diphosphate

Alternate protein names: PBP-2 [H]

Number of amino acids: Translated: 615; Mature: 615

Protein sequence:

>615_residues
MKPSQKRQNVKFSSSPVLGVHLPMWRSKLVVFMLFMAFVALAARAFWIQGPGNAFYRKQGESRYQRTLDLPATRGKILDR
NGLVLATSLPVRAIWAIPDAVPDDLDADKVNQLGKLLGMTSKELRVKLSEDKGFVYVKRQVPIDVADKVAALDIPGIYQR
NEYKRFYPEGEITAHLIGFTNVEDEGQEGVELGDQKMLSGTSGVRRVIKDRMGHIVEDVAEQIPPHNGTDVDLSIDSKIQ
YIAYANLKAAVEKFKAKAGAAMVVDVRTGEVLALVNYPTYNPNDRSRMTGEQLRNRIMTDVFEPGSIMKPFTVSLALDLH
RVTPNTLVETGNGHFVLDGAPITDDAGFGTLTVGGVIQKSSNIGATKIAMTMRPEEMWNMYTSIGLGQAPKVGFPGAVAG
RLRPWKSWRRIEQATMSYGYGLSVSLFQLARAYTAIAHDGELMPVTIFKTDPNQPVVGTQVFNPTTAREVRAMLETVVAP
GGTSPDAAVPGYRVGGKSGTAYKHEGHGYTRKYRASFVGMAPMPNPRIVVAVSVDEPTAGSHFGGQVSGPVFSAIAGDTM
RALNVPPNMPIKQLVVSDDSPAAPSATGPQKLAAGSGAKHMIVSSTTRNSPGVVR

Sequences:

>Translated_615_residues
MKPSQKRQNVKFSSSPVLGVHLPMWRSKLVVFMLFMAFVALAARAFWIQGPGNAFYRKQGESRYQRTLDLPATRGKILDR
NGLVLATSLPVRAIWAIPDAVPDDLDADKVNQLGKLLGMTSKELRVKLSEDKGFVYVKRQVPIDVADKVAALDIPGIYQR
NEYKRFYPEGEITAHLIGFTNVEDEGQEGVELGDQKMLSGTSGVRRVIKDRMGHIVEDVAEQIPPHNGTDVDLSIDSKIQ
YIAYANLKAAVEKFKAKAGAAMVVDVRTGEVLALVNYPTYNPNDRSRMTGEQLRNRIMTDVFEPGSIMKPFTVSLALDLH
RVTPNTLVETGNGHFVLDGAPITDDAGFGTLTVGGVIQKSSNIGATKIAMTMRPEEMWNMYTSIGLGQAPKVGFPGAVAG
RLRPWKSWRRIEQATMSYGYGLSVSLFQLARAYTAIAHDGELMPVTIFKTDPNQPVVGTQVFNPTTAREVRAMLETVVAP
GGTSPDAAVPGYRVGGKSGTAYKHEGHGYTRKYRASFVGMAPMPNPRIVVAVSVDEPTAGSHFGGQVSGPVFSAIAGDTM
RALNVPPNMPIKQLVVSDDSPAAPSATGPQKLAAGSGAKHMIVSSTTRNSPGVVR
>Mature_615_residues
MKPSQKRQNVKFSSSPVLGVHLPMWRSKLVVFMLFMAFVALAARAFWIQGPGNAFYRKQGESRYQRTLDLPATRGKILDR
NGLVLATSLPVRAIWAIPDAVPDDLDADKVNQLGKLLGMTSKELRVKLSEDKGFVYVKRQVPIDVADKVAALDIPGIYQR
NEYKRFYPEGEITAHLIGFTNVEDEGQEGVELGDQKMLSGTSGVRRVIKDRMGHIVEDVAEQIPPHNGTDVDLSIDSKIQ
YIAYANLKAAVEKFKAKAGAAMVVDVRTGEVLALVNYPTYNPNDRSRMTGEQLRNRIMTDVFEPGSIMKPFTVSLALDLH
RVTPNTLVETGNGHFVLDGAPITDDAGFGTLTVGGVIQKSSNIGATKIAMTMRPEEMWNMYTSIGLGQAPKVGFPGAVAG
RLRPWKSWRRIEQATMSYGYGLSVSLFQLARAYTAIAHDGELMPVTIFKTDPNQPVVGTQVFNPTTAREVRAMLETVVAP
GGTSPDAAVPGYRVGGKSGTAYKHEGHGYTRKYRASFVGMAPMPNPRIVVAVSVDEPTAGSHFGGQVSGPVFSAIAGDTM
RALNVPPNMPIKQLVVSDDSPAAPSATGPQKLAAGSGAKHMIVSSTTRNSPGVVR

Specific function: Synthesis of cross-linked peptidoglycan from the lipid intermediates [H]

COG id: COG0768

COG function: function code M; Cell division protein FtsI/penicillin-binding protein 2

Gene ontology:

Cell location: Cell inner membrane; Peripheral membrane protein [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the transpeptidase family [H]

Homologues:

Organism=Escherichia coli, GI1786272, Length=575, Percent_Identity=40.8695652173913, Blast_Score=402, Evalue=1e-113,
Organism=Escherichia coli, GI1786854, Length=602, Percent_Identity=23.421926910299, Blast_Score=102, Evalue=6e-23,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012338
- InterPro:   IPR005311
- InterPro:   IPR001460 [H]

Pfam domain/function: PF03717 PBP_dimer; PF00905 Transpeptidase [H]

EC number: 2.4.1.129

Molecular weight: Translated: 66594; Mature: 66594

Theoretical pI: Translated: 9.97; Mature: 9.97

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
3.7 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
3.7 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKPSQKRQNVKFSSSPVLGVHLPMWRSKLVVFMLFMAFVALAARAFWIQGPGNAFYRKQG
CCCCCCCCCCEECCCCEEEEECCHHHHHHHHHHHHHHHHHHHHHEEEEECCCCHHHHCCC
ESRYQRTLDLPATRGKILDRNGLVLATSLPVRAIWAIPDAVPDDLDADKVNQLGKLLGMT
HHHHHHEECCCCCCCCEECCCCEEEEECCCEEEEEECCCCCCCCCCHHHHHHHHHHHCCC
SKELRVKLSEDKGFVYVKRQVPIDVADKVAALDIPGIYQRNEYKRFYPEGEITAHLIGFT
CCCEEEEEECCCCEEEEEEECCCCHHCCEEEECCCCCCCCCCCHHCCCCCCEEEEEEEEC
NVEDEGQEGVELGDQKMLSGTSGVRRVIKDRMGHIVEDVAEQIPPHNGTDVDLSIDSKIQ
CCCCCCCCCHHCCCHHHHCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEEECCCEE
YIAYANLKAAVEKFKAKAGAAMVVDVRTGEVLALVNYPTYNPNDRSRMTGEQLRNRIMTD
EEEEHHHHHHHHHHHHCCCCEEEEEECCCCEEEEEECCCCCCCCHHHCCHHHHHHHHHHH
VFEPGSIMKPFTVSLALDLHRVTPNTLVETGNGHFVLDGAPITDDAGFGTLTVGGVIQKS
HCCCCCCCCCEEEEEEEEEEECCCCCEEECCCCEEEECCCCCCCCCCCCEEEECCEEECC
SNIGATKIAMTMRPEEMWNMYTSIGLGQAPKVGFPGAVAGRLRPWKSWRRIEQATMSYGY
CCCCCEEEEEEECHHHHHHHHHHCCCCCCCCCCCCCHHHCCCCCHHHHHHHHHHHHHCCC
GLSVSLFQLARAYTAIAHDGELMPVTIFKTDPNQPVVGTQVFNPTTAREVRAMLETVVAP
CCHHHHHHHHHHHHHHHCCCCEEEEEEEECCCCCCEEEEEECCCCHHHHHHHHHHHHHCC
GGTSPDAAVPGYRVGGKSGTAYKHEGHGYTRKYRASFVGMAPMPNPRIVVAVSVDEPTAG
CCCCCCCCCCCEEECCCCCCCEEECCCCCCCEEHHHEEECCCCCCCCEEEEEEECCCCCC
SHFGGQVSGPVFSAIAGDTMRALNVPPNMPIKQLVVSDDSPAAPSATGPQKLAAGSGAKH
CCCCCCCCCHHHHHHHCCCCEEECCCCCCCHHHEEECCCCCCCCCCCCCHHHHCCCCCCE
MIVSSTTRNSPGVVR
EEEEECCCCCCCCCC
>Mature Secondary Structure
MKPSQKRQNVKFSSSPVLGVHLPMWRSKLVVFMLFMAFVALAARAFWIQGPGNAFYRKQG
CCCCCCCCCCEECCCCEEEEECCHHHHHHHHHHHHHHHHHHHHHEEEEECCCCHHHHCCC
ESRYQRTLDLPATRGKILDRNGLVLATSLPVRAIWAIPDAVPDDLDADKVNQLGKLLGMT
HHHHHHEECCCCCCCCEECCCCEEEEECCCEEEEEECCCCCCCCCCHHHHHHHHHHHCCC
SKELRVKLSEDKGFVYVKRQVPIDVADKVAALDIPGIYQRNEYKRFYPEGEITAHLIGFT
CCCEEEEEECCCCEEEEEEECCCCHHCCEEEECCCCCCCCCCCHHCCCCCCEEEEEEEEC
NVEDEGQEGVELGDQKMLSGTSGVRRVIKDRMGHIVEDVAEQIPPHNGTDVDLSIDSKIQ
CCCCCCCCCHHCCCHHHHCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEEECCCEE
YIAYANLKAAVEKFKAKAGAAMVVDVRTGEVLALVNYPTYNPNDRSRMTGEQLRNRIMTD
EEEEHHHHHHHHHHHHCCCCEEEEEECCCCEEEEEECCCCCCCCHHHCCHHHHHHHHHHH
VFEPGSIMKPFTVSLALDLHRVTPNTLVETGNGHFVLDGAPITDDAGFGTLTVGGVIQKS
HCCCCCCCCCEEEEEEEEEEECCCCCEEECCCCEEEECCCCCCCCCCCCEEEECCEEECC
SNIGATKIAMTMRPEEMWNMYTSIGLGQAPKVGFPGAVAGRLRPWKSWRRIEQATMSYGY
CCCCCEEEEEEECHHHHHHHHHHCCCCCCCCCCCCCHHHCCCCCHHHHHHHHHHHHHCCC
GLSVSLFQLARAYTAIAHDGELMPVTIFKTDPNQPVVGTQVFNPTTAREVRAMLETVVAP
CCHHHHHHHHHHHHHHHCCCCEEEEEEEECCCCCCEEEEEECCCCHHHHHHHHHHHHHCC
GGTSPDAAVPGYRVGGKSGTAYKHEGHGYTRKYRASFVGMAPMPNPRIVVAVSVDEPTAG
CCCCCCCCCCCEEECCCCCCCEEECCCCCCCEEHHHEEECCCCCCCCEEEEEEECCCCCC
SHFGGQVSGPVFSAIAGDTMRALNVPPNMPIKQLVVSDDSPAAPSATGPQKLAAGSGAKH
CCCCCCCCCHHHHHHHCCCCEEECCCCCCCHHHEEECCCCCCCCCCCCCHHHHCCCCCCE
MIVSSTTRNSPGVVR
EEEEECCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: [GlcNAc-(1->4)-Mur2Ac(oyl-L-Ala-gamma-D-Glu-L-Lys-D-Ala-D-Ala)]n-; $diphosphoundecaprenol; GlcNAc-(1->4)-Mur2Ac(oyl-L-Ala-gamma-D-Glu-L-Lys-D-Ala-D-Ala)-; $diphosphoundecaprenol

Specific reaction: [GlcNAc-(1->4)-Mur2Ac(oyl-L-Ala-gamma-D-Glu-L-Lys-D-Ala-D-Ala)]n- $diphosphoundecaprenol + GlcNAc-(1->4)-Mur2Ac(oyl-L-Ala-gamma-D-Glu- $L-Lys-D-Ala-D-Ala)-diphosphoundecaprenol = [GlcNAc-(1->4)- $Mur2Ac(oyl-L-Ala-gamma-

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 3126399 [H]