| Definition | Burkholderia sp. 383 chromosome 1, complete genome. |
|---|---|
| Accession | NC_007510 |
| Length | 3,694,126 |
Click here to switch to the map view.
The map label for this gene is mrcA [H]
Identifier: 78064963
GI number: 78064963
Start: 348154
End: 350544
Strand: Reverse
Name: mrcA [H]
Synonym: Bcep18194_A3486
Alternate gene names: 78064963
Gene position: 350544-348154 (Counterclockwise)
Preceding gene: 78064974
Following gene: 78064955
Centisome position: 9.49
GC content: 66.42
Gene sequence:
>2391_bases ATGCCTATGCAATCCACGACTCCTACGTCCCCGCCTCCCGCCCCGGAGCCGAAGAAGCGGCCCTGGTGGCAAAAAGTCCT GCTCGGATTCGCCACGATGTGCGTGGCGCTCGTCGTGGCCGGCGGCCTCGTGCTCGGCTACGCGCTCGTCGTCGCCTGGC CGAACATGCCTTCGCTCGACGCGCTGACCGACTATCGGCCGAAGGTGCCGCTGCGCATCTATACGTCCGACCACGTGCTG ATCGGCGAATTCGGCGAAGAGCGCCGCGACGTCGTCCATTTCAAGGACGTGCCCGACTCGCTGAAGAATGCGATCCTCGC GATCGAGGACGCGCGCTTCTACGATCACGGCGGCGTCGACCTCACCGGCATCGTCCGCGCCGGCTTCGTCGCGCTGACGA ACGGCCATGCGTCGCAGGGCGCGAGCACGATCACGATGCAGGTCGCGCGCAACTTCTTCCTGTCGAGCGAGAAGACCTAC ACGCGCAAGATCTACGAGATGCTGCTCGCGTACCGGATCGAGCGTGCGCTGACGAAGGATCAGATTCTCGAGGTCTACAT GAATCAGATCTATCTCGGCCAGCGCGCGTACGGCTTCGCGAGCGCCGCGCGGGTCTATTTCGGCAAGGACCTGAAGGACA TCACGCTCGCGGAAGCCGCGATGCTCGCGGGGCTGCCGAAGGCGCCGTCCGCATATAACCCGGTCGTGAACCCGAAGCGC GCGAAGGTGCGCCAGGAATACATCCTGCAACGGATGCTCGAGCTGAACTTCATCTCGCGCGAGCAGTACGACGAGGCCGT CGCACAGCCGCTCGTCGTCAAGGGTCCGGGCCGCGATTTCAGCGTGCACGCCGAGTACGTCGCGGAAATGGTCCGACAAA TGATGTACGCGCAGTACCGTGAGGAAACCTACACGCGCGGCTTCAACGTCGTCACGACGATCGACTCCGCCGACCAGCAG GTCGCGTACACCGCGCTGCGCAAGGGCATCATGGATTACGAACGCCGGCACGGCTATCGGGGGCCGGAAGGCTTCATCGA GCTGCCGGCCGGTGCCGACGACCGCGAACAGGCGATCGACGATGCGCTGCTCGAACACCCCGACAACGGTGAGCTGATTG CCGCGGTCGTCACGGCCGCCAGCCCGCGCCAGATCACGGTTGCGTTCATCGACGGCAGCAATGCGACGATCGAAGGCGAC AACCTGCGCTTCGCGTCGGGCGCGCTGTCGGCCAATGCGCAGCCGAATCGCCGCATCCGCCCGGGCGCGATCGTCCGCGT CGTGAAGAACGACACCGGCAAATGGTCGATCACGCAGTTGCCGCAGGTCGAGGGGGCGTTCCTGTCGATCGTCCCGCAGG ACGGCGCGATCCGCTCGCTCGTCGGCGGCTTCGACTACAACAAGAACAAGTTCAACCACGTCACGCAGGCGTGGCGGCAA CCGGGTTCGTCGTTCAAGCCGTTCATCTATTCGGCGTCGCTCGACAAGGGCCTCGGGCCGGCAACCGTGATCAACGACGG CCCGCTGTATTTCAGCGCCGCCGAAACGGGCGGCCAGCCGTGGGAGCCGAAGAACTACGGTGGCGGCTTCGAAGGCCCGA TGTCGATGCGCACCGCGCTGCAGCGCTCGCGCAACCTCGTGTCGATCCGGATCCTGAACCACATCGGCACGAAGTACGCG CAGCAGTACATCACGCGCTTCGGCTTCGACGCCGAACGCCATCCGGCCTACCTGCCGATGGCACTCGGCGCGGGCCAGGT CACGCCGCTGCAGATGGCGGGCGCGTACTCGGTCTTCGCGAACGGCGGCTACCGCGTCAATCCGTACCTGATCGCCGAAG TCACCGATCCGAACGGCGCGATCGTCGTGCGCGCGCAGCCGCTCGTCGCCGAGCAGAACGCGCCGCGTGCGATCGACGCG CGCAATGCGTACGTGATGAACAGCCTGCTGCAGAGCGTCGCGCAGCGCGGCACGGGCGCGCGGACCAACGTGCTGAAGCG CACCGACCTCGCGGGCAAGACCGGCACGACGAACGACTCGCACGACGCGTGGTTCGCGGGCTACCAGCACACGCTCGCCG CGATTGCGTGGATCGGCTACGACAACCCGCGCAGCCTCGGCGATCGCGAAACGGGGGGCGGCCTGTCGCTGCCGGTCTGG ATCGACTACATGGGCGCCGCGCTGAAGGGCGTGCCCGAGTTCAAGCCGACCGCGCCGGACGGCGTCGAGTCGCTCGGCGG CGAGCTGTACTTCACCGAGTTCGCGCCGGGCCACGGCTTCGTGTCGACGGTTGGCGTACCGCAAGCACCGGCCGCGCAGA ACGTCGACGCAACCGTGCCGCACGTCGACGAGCAGGAAAAGCAGGACATCATGAACCTGTTCGCGCACTGA
Upstream 100 bases:
>100_bases GCTATGCTGGAAGGACGCACACCCGCGGCTTCGCGGCGGGTAGCCCCCGCGTCAGGACCGGTCTTACCGGGCAGCTATAA TCGCGGGACTGTTTTCCGGT
Downstream 100 bases:
>100_bases TCACCGGCCCGGACATAAAAAAAGCGCCCCGCGGGGCGCTTTTTCTTTGGCACGGGCGCATCGGGACGCGGCCCCGGCTT CGGCCTCGGCGGTTACGGCG
Product: penicillin-binding protein 1A
Products: NA
Alternate protein names: PBP-1a; PBP1a; Penicillin-insensitive transglycosylase; Peptidoglycan TGase; Penicillin-sensitive transpeptidase; DD-transpeptidase [H]
Number of amino acids: Translated: 796; Mature: 795
Protein sequence:
>796_residues MPMQSTTPTSPPPAPEPKKRPWWQKVLLGFATMCVALVVAGGLVLGYALVVAWPNMPSLDALTDYRPKVPLRIYTSDHVL IGEFGEERRDVVHFKDVPDSLKNAILAIEDARFYDHGGVDLTGIVRAGFVALTNGHASQGASTITMQVARNFFLSSEKTY TRKIYEMLLAYRIERALTKDQILEVYMNQIYLGQRAYGFASAARVYFGKDLKDITLAEAAMLAGLPKAPSAYNPVVNPKR AKVRQEYILQRMLELNFISREQYDEAVAQPLVVKGPGRDFSVHAEYVAEMVRQMMYAQYREETYTRGFNVVTTIDSADQQ VAYTALRKGIMDYERRHGYRGPEGFIELPAGADDREQAIDDALLEHPDNGELIAAVVTAASPRQITVAFIDGSNATIEGD NLRFASGALSANAQPNRRIRPGAIVRVVKNDTGKWSITQLPQVEGAFLSIVPQDGAIRSLVGGFDYNKNKFNHVTQAWRQ PGSSFKPFIYSASLDKGLGPATVINDGPLYFSAAETGGQPWEPKNYGGGFEGPMSMRTALQRSRNLVSIRILNHIGTKYA QQYITRFGFDAERHPAYLPMALGAGQVTPLQMAGAYSVFANGGYRVNPYLIAEVTDPNGAIVVRAQPLVAEQNAPRAIDA RNAYVMNSLLQSVAQRGTGARTNVLKRTDLAGKTGTTNDSHDAWFAGYQHTLAAIAWIGYDNPRSLGDRETGGGLSLPVW IDYMGAALKGVPEFKPTAPDGVESLGGELYFTEFAPGHGFVSTVGVPQAPAAQNVDATVPHVDEQEKQDIMNLFAH
Sequences:
>Translated_796_residues MPMQSTTPTSPPPAPEPKKRPWWQKVLLGFATMCVALVVAGGLVLGYALVVAWPNMPSLDALTDYRPKVPLRIYTSDHVL IGEFGEERRDVVHFKDVPDSLKNAILAIEDARFYDHGGVDLTGIVRAGFVALTNGHASQGASTITMQVARNFFLSSEKTY TRKIYEMLLAYRIERALTKDQILEVYMNQIYLGQRAYGFASAARVYFGKDLKDITLAEAAMLAGLPKAPSAYNPVVNPKR AKVRQEYILQRMLELNFISREQYDEAVAQPLVVKGPGRDFSVHAEYVAEMVRQMMYAQYREETYTRGFNVVTTIDSADQQ VAYTALRKGIMDYERRHGYRGPEGFIELPAGADDREQAIDDALLEHPDNGELIAAVVTAASPRQITVAFIDGSNATIEGD NLRFASGALSANAQPNRRIRPGAIVRVVKNDTGKWSITQLPQVEGAFLSIVPQDGAIRSLVGGFDYNKNKFNHVTQAWRQ PGSSFKPFIYSASLDKGLGPATVINDGPLYFSAAETGGQPWEPKNYGGGFEGPMSMRTALQRSRNLVSIRILNHIGTKYA QQYITRFGFDAERHPAYLPMALGAGQVTPLQMAGAYSVFANGGYRVNPYLIAEVTDPNGAIVVRAQPLVAEQNAPRAIDA RNAYVMNSLLQSVAQRGTGARTNVLKRTDLAGKTGTTNDSHDAWFAGYQHTLAAIAWIGYDNPRSLGDRETGGGLSLPVW IDYMGAALKGVPEFKPTAPDGVESLGGELYFTEFAPGHGFVSTVGVPQAPAAQNVDATVPHVDEQEKQDIMNLFAH >Mature_795_residues PMQSTTPTSPPPAPEPKKRPWWQKVLLGFATMCVALVVAGGLVLGYALVVAWPNMPSLDALTDYRPKVPLRIYTSDHVLI GEFGEERRDVVHFKDVPDSLKNAILAIEDARFYDHGGVDLTGIVRAGFVALTNGHASQGASTITMQVARNFFLSSEKTYT RKIYEMLLAYRIERALTKDQILEVYMNQIYLGQRAYGFASAARVYFGKDLKDITLAEAAMLAGLPKAPSAYNPVVNPKRA KVRQEYILQRMLELNFISREQYDEAVAQPLVVKGPGRDFSVHAEYVAEMVRQMMYAQYREETYTRGFNVVTTIDSADQQV AYTALRKGIMDYERRHGYRGPEGFIELPAGADDREQAIDDALLEHPDNGELIAAVVTAASPRQITVAFIDGSNATIEGDN LRFASGALSANAQPNRRIRPGAIVRVVKNDTGKWSITQLPQVEGAFLSIVPQDGAIRSLVGGFDYNKNKFNHVTQAWRQP GSSFKPFIYSASLDKGLGPATVINDGPLYFSAAETGGQPWEPKNYGGGFEGPMSMRTALQRSRNLVSIRILNHIGTKYAQ QYITRFGFDAERHPAYLPMALGAGQVTPLQMAGAYSVFANGGYRVNPYLIAEVTDPNGAIVVRAQPLVAEQNAPRAIDAR NAYVMNSLLQSVAQRGTGARTNVLKRTDLAGKTGTTNDSHDAWFAGYQHTLAAIAWIGYDNPRSLGDRETGGGLSLPVWI DYMGAALKGVPEFKPTAPDGVESLGGELYFTEFAPGHGFVSTVGVPQAPAAQNVDATVPHVDEQEKQDIMNLFAH
Specific function: Cell wall formation. Synthesis of cross-linked peptidoglycan from the lipid intermediates. The enzyme has a penicillin-insensitive transglycosylase N-terminal domain (formation of linear glycan strands) and a penicillin-sensitive transpeptidase C-terminal
COG id: COG5009
COG function: function code M; Membrane carboxypeptidase/penicillin-binding protein
Gene ontology:
Cell location: Cell inner membrane; Single-pass type II membrane protein [H]
Metaboloic importance: Non_Essential [C]
Operon status: Not Known
Operon components: None
Similarity: In the C-terminal section; belongs to the transpeptidase family [H]
Homologues:
Organism=Escherichia coli, GI87082258, Length=803, Percent_Identity=38.2316313823163, Blast_Score=522, Evalue=1e-149, Organism=Escherichia coli, GI1786343, Length=249, Percent_Identity=38.5542168674699, Blast_Score=150, Evalue=2e-37, Organism=Escherichia coli, GI1788867, Length=229, Percent_Identity=32.3144104803493, Blast_Score=117, Evalue=4e-27, Organism=Escherichia coli, GI1789601, Length=178, Percent_Identity=32.5842696629214, Blast_Score=91, Evalue=4e-19,
Paralogues:
None
Copy number: NA
Swissprot (AC and ID): NA
Other databases:
- InterPro: IPR012338 - InterPro: IPR001264 - InterPro: IPR011816 - InterPro: IPR001460 [H]
Pfam domain/function: PF00912 Transgly; PF00905 Transpeptidase [H]
EC number: 2.4.2.-; 3.4.-.- [C]
Molecular weight: Translated: 87212; Mature: 87081
Theoretical pI: Translated: 7.09; Mature: 7.09
Prosite motif: NA
Important sites: NA
Signals:
None
Transmembrane regions:
None
Cys/Met content:
0.1 %Cys (Translated Protein) 2.5 %Met (Translated Protein) 2.6 %Cys+Met (Translated Protein) 0.1 %Cys (Mature Protein) 2.4 %Met (Mature Protein) 2.5 %Cys+Met (Mature Protein)
Secondary structure:
>Translated Secondary Structure MPMQSTTPTSPPPAPEPKKRPWWQKVLLGFATMCVALVVAGGLVLGYALVVAWPNMPSLD CCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCH ALTDYRPKVPLRIYTSDHVLIGEFGEERRDVVHFKDVPDSLKNAILAIEDARFYDHGGVD HHHCCCCCCCEEEEECCEEEEECCCCCHHCEEEECCCCHHHHHHEEEEECCEEECCCCCC LTGIVRAGFVALTNGHASQGASTITMQVARNFFLSSEKTYTRKIYEMLLAYRIERALTKD HHHHHHCCEEEEECCCCCCCCHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHH QILEVYMNQIYLGQRAYGFASAARVYFGKDLKDITLAEAAMLAGLPKAPSAYNPVVNPKR HHHHHHHHHHHHCCHHHCHHHHHHHHHCCCCHHHHHHHHHHHHCCCCCCCCCCCCCCCHH AKVRQEYILQRMLELNFISREQYDEAVAQPLVVKGPGRDFSVHAEYVAEMVRQMMYAQYR HHHHHHHHHHHHHHHHHHHHHHHHHHHHCCEEEECCCCCCEECHHHHHHHHHHHHHHHHH EETYTRGFNVVTTIDSADQQVAYTALRKGIMDYERRHGYRGPEGFIELPAGADDREQAID HHHHHCCCEEEEEECCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCEECCCCCCHHHHHHH DALLEHPDNGELIAAVVTAASPRQITVAFIDGSNATIEGDNLRFASGALSANAQPNRRIR HHHHCCCCCCCEEEEEEECCCCCEEEEEEEECCCCEEECCCEEEECCCCCCCCCCCCCCC PGAIVRVVKNDTGKWSITQLPQVEGAFLSIVPQDGAIRSLVGGFDYNKNKFNHVTQAWRQ CCCEEEEEECCCCCEEEECCCCCCCCEEEEECCCCHHHHHHCCCCCCCCHHHHHHHHHHC PGSSFKPFIYSASLDKGLGPATVINDGPLYFSAAETGGQPWEPKNYGGGFEGPMSMRTAL CCCCCCCEEEECCCCCCCCCEEEECCCCEEEEECCCCCCCCCCCCCCCCCCCHHHHHHHH QRSRNLVSIRILNHIGTKYAQQYITRFGFDAERHPAYLPMALGAGQVTPLQMAGAYSVFA HHCCCEEEEEHHHHHHHHHHHHHHHHHCCCCCCCCCEEEEECCCCCCCCCHHCCCEEEEE NGGYRVNPYLIAEVTDPNGAIVVRAQPLVAEQNAPRAIDARNAYVMNSLLQSVAQRGTGA CCCEEECCEEEEEEECCCCEEEEEECCEEECCCCCCEECCCHHHHHHHHHHHHHHCCCCC RTNVLKRTDLAGKTGTTNDSHDAWFAGYQHTLAAIAWIGYDNPRSLGDRETGGGLSLPVW HHHHHHHCCCCCCCCCCCCCCCHHHHHHHHHHHHHHEECCCCCCCCCCCCCCCCCCCHHH IDYMGAALKGVPEFKPTAPDGVESLGGELYFTEFAPGHGFVSTVGVPQAPAAQNVDATVP HHHHHHHHCCCCCCCCCCCCHHHHCCCEEEEEEECCCCCCEEECCCCCCCCCCCCCCCCC HVDEQEKQDIMNLFAH CCCCHHHHHHHHHHCC >Mature Secondary Structure PMQSTTPTSPPPAPEPKKRPWWQKVLLGFATMCVALVVAGGLVLGYALVVAWPNMPSLD CCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCH ALTDYRPKVPLRIYTSDHVLIGEFGEERRDVVHFKDVPDSLKNAILAIEDARFYDHGGVD HHHCCCCCCCEEEEECCEEEEECCCCCHHCEEEECCCCHHHHHHEEEEECCEEECCCCCC LTGIVRAGFVALTNGHASQGASTITMQVARNFFLSSEKTYTRKIYEMLLAYRIERALTKD HHHHHHCCEEEEECCCCCCCCHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHH QILEVYMNQIYLGQRAYGFASAARVYFGKDLKDITLAEAAMLAGLPKAPSAYNPVVNPKR HHHHHHHHHHHHCCHHHCHHHHHHHHHCCCCHHHHHHHHHHHHCCCCCCCCCCCCCCCHH AKVRQEYILQRMLELNFISREQYDEAVAQPLVVKGPGRDFSVHAEYVAEMVRQMMYAQYR HHHHHHHHHHHHHHHHHHHHHHHHHHHHCCEEEECCCCCCEECHHHHHHHHHHHHHHHHH EETYTRGFNVVTTIDSADQQVAYTALRKGIMDYERRHGYRGPEGFIELPAGADDREQAID HHHHHCCCEEEEEECCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCEECCCCCCHHHHHHH DALLEHPDNGELIAAVVTAASPRQITVAFIDGSNATIEGDNLRFASGALSANAQPNRRIR HHHHCCCCCCCEEEEEEECCCCCEEEEEEEECCCCEEECCCEEEECCCCCCCCCCCCCCC PGAIVRVVKNDTGKWSITQLPQVEGAFLSIVPQDGAIRSLVGGFDYNKNKFNHVTQAWRQ CCCEEEEEECCCCCEEEECCCCCCCCEEEEECCCCHHHHHHCCCCCCCCHHHHHHHHHHC PGSSFKPFIYSASLDKGLGPATVINDGPLYFSAAETGGQPWEPKNYGGGFEGPMSMRTAL CCCCCCCEEEECCCCCCCCCEEEECCCCEEEEECCCCCCCCCCCCCCCCCCCHHHHHHHH QRSRNLVSIRILNHIGTKYAQQYITRFGFDAERHPAYLPMALGAGQVTPLQMAGAYSVFA HHCCCEEEEEHHHHHHHHHHHHHHHHHCCCCCCCCCEEEEECCCCCCCCCHHCCCEEEEE NGGYRVNPYLIAEVTDPNGAIVVRAQPLVAEQNAPRAIDARNAYVMNSLLQSVAQRGTGA CCCEEECCEEEEEEECCCCEEEEEECCEEECCCCCCEECCCHHHHHHHHHHHHHHCCCCC RTNVLKRTDLAGKTGTTNDSHDAWFAGYQHTLAAIAWIGYDNPRSLGDRETGGGLSLPVW HHHHHHHCCCCCCCCCCCCCCCHHHHHHHHHHHHHHEECCCCCCCCCCCCCCCCCCCHHH IDYMGAALKGVPEFKPTAPDGVESLGGELYFTEFAPGHGFVSTVGVPQAPAAQNVDATVP HHHHHHHHCCCCCCCCCCCCHHHHCCCEEEEEEECCCCCCEEECCCCCCCCCCCCCCCCC HVDEQEKQDIMNLFAH CCCCHHHHHHHHHHCC
PDB accession: NA
Resolution: NA
Structure class: Alpha Beta
Cofactors: NA
Metal ions: NA
Kcat value (1/min): NA
Specific activity: NA
Km value (mM): NA
Substrates: NA
Specific reaction: NA
General reaction: Transferases; Acyltransferases; Aminoacyltransferases [C]
Inhibitor: NA
Structure determination priority: 6.0
TargetDB status: NA
Availability: NA
References: NA