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The map label for this gene is nylA [H]

Identifier: 78064855

GI number: 78064855

Start: 236806

End: 238284

Strand: Reverse

Name: nylA [H]

Synonym: Bcep18194_A3378

Alternate gene names: 78064855

Gene position: 238284-236806 (Counterclockwise)

Preceding gene: 78064856

Following gene: 78064829

Centisome position: 6.45

GC content: 71.74

Gene sequence:

>1479_bases
ATGCACTCGGACTATCTCGCGCATGACGCGATCGGCCTCGCCACACTGGTACGCGAACGCAAGGCGAGCCCGCGCGAACT
GCTCGACGCCGCGATCGGCCAGGCCGAAGCGGTCAACAGTGCGATCAACGCGATCGTGCTGCAGGACTACGAAGCCGCAC
GCCAGCGTGCGGCCGCCACACCCGACGGCGGCGCCGACGCGCCGTTCGCCGGCGTTCCCTATCTCGTGAAGGATCTCGGC
GCGGCGGTCGCCGGGCTGCCGCTGTCGATGGGCAGCCGGCACTACCGGTACTTCGTGCCCGCCGACGATTCGCCGGTGAT
CGCGCGCAGCCGCGCGGCCGGTCTCAACGTGTTCGGCAAGACCAACACGTCGGAGATCGGCCAGATGCCGTACACGGAAC
CCGAACTGTTCGGCGCGTGCCGCAACCCGTGGAATCTCGACCACACGCCCGGCGGTTCGAGCGGCGGTGCGGCGGCGGCC
GTCGCGGCCGGCATCGTGCCGATCGCGCATGCGTCCGACGGCGGCGGCTCGATCCGCATTCCGGCGTCGTGCTGCGGGCT
GTTCGGCCTGAAGCCGAGCCGCAACCCGGTACTCGTCGACCTGCCTTCGAACGGCGAGCTGGTCGTCCAGCATGCGGTCA
CGCGCAGCGTGCGCGACAGCGCGCTGCTGCTCGACGTGACGACCGGCCAGACCTTGCCGGCCGGCGCGCCCGGCACGTTC
CTCGGCGAGCTCGACACGCCGCCCGGCCCGCTGCGGATCGGCCTCGTGCTCGACCCGATGCTTGCGCCGGCGCTCGCCGA
CGACACGCGCGCGGCGCTCGACGACGCGGCCGCGCTGCTCGAATCGCTCGGCCACCATGTCGAGCCGGCGACGCTGCAAG
TCAACTACGCGCGCGCGGCCGAAACCTTCCTCACGCTGTGGGCGACGATCGCCGAGGAGATGGTGCTGGGCGCACGCGCG
CTGACCGGCCGCACGCCGAAGCGCGGCGAATTCGAAGCCGCGACGTGGGCGATGGCCGTGGTCGGCCGGCGCCTCGCGCG
CACGCGCCTGCCGGACGTGCTCGAATGGCAGCGCCAGCTCACCGTGCAGGTGGCCGGCCTCGTGTCGCGCTACGACGCGA
TCCTGTGCGCGTCGCTCGCGGGCCCGCCGGTGAAGATCGGCGAGTTGCAGCCGACGCCGTTCGAAACGACGCAGATGAAG
GTGCTCGCCGCACTGCCGGTGAAACCGCTGCTGAAGGAAATGCTCGCGAAGTCGTCGGAAAAGGCGTTCACGTGGGCCGG
CTGCACCGAGCTGTTCAATCTGACCGGCCAGCCGGCGATGTCGGTGCCGCTCTACTGGAACGCGCGCGGGCTGCCGATCG
GCGTGCAGTTCGCCGCGCGTCACGCCGACGACGCGCTGCTGCTCCGGCTCGCCCGGCAGCTCGAACAGGCGCGGCCGTGG
TTCGAGCGCCGCCCGCCGCTGATGCAGGCGCAGCGCTGA

Upstream 100 bases:

>100_bases
CCTCGCCGAGCACCTCTAAAAACGGCCGGCCGCGGGCCCCGCGCACCGGGCCCGCGTTATGCTGCTGTCATCGCCACGGG
TCACAACGGGGGTGACTGCG

Downstream 100 bases:

>100_bases
CGCGCGCGGCCGCGCTCCGGAGGTCTCGAAAAAAAAGCCCCGCCGGCATCACCGGCGGGGCTTTTACGCATGCCATCGCG
TGAGCGCGCTTACACGACGA

Product: amidase

Products: NA

Alternate protein names: Nylon oligomers-degrading enzyme EI [H]

Number of amino acids: Translated: 492; Mature: 492

Protein sequence:

>492_residues
MHSDYLAHDAIGLATLVRERKASPRELLDAAIGQAEAVNSAINAIVLQDYEAARQRAAATPDGGADAPFAGVPYLVKDLG
AAVAGLPLSMGSRHYRYFVPADDSPVIARSRAAGLNVFGKTNTSEIGQMPYTEPELFGACRNPWNLDHTPGGSSGGAAAA
VAAGIVPIAHASDGGGSIRIPASCCGLFGLKPSRNPVLVDLPSNGELVVQHAVTRSVRDSALLLDVTTGQTLPAGAPGTF
LGELDTPPGPLRIGLVLDPMLAPALADDTRAALDDAAALLESLGHHVEPATLQVNYARAAETFLTLWATIAEEMVLGARA
LTGRTPKRGEFEAATWAMAVVGRRLARTRLPDVLEWQRQLTVQVAGLVSRYDAILCASLAGPPVKIGELQPTPFETTQMK
VLAALPVKPLLKEMLAKSSEKAFTWAGCTELFNLTGQPAMSVPLYWNARGLPIGVQFAARHADDALLLRLARQLEQARPW
FERRPPLMQAQR

Sequences:

>Translated_492_residues
MHSDYLAHDAIGLATLVRERKASPRELLDAAIGQAEAVNSAINAIVLQDYEAARQRAAATPDGGADAPFAGVPYLVKDLG
AAVAGLPLSMGSRHYRYFVPADDSPVIARSRAAGLNVFGKTNTSEIGQMPYTEPELFGACRNPWNLDHTPGGSSGGAAAA
VAAGIVPIAHASDGGGSIRIPASCCGLFGLKPSRNPVLVDLPSNGELVVQHAVTRSVRDSALLLDVTTGQTLPAGAPGTF
LGELDTPPGPLRIGLVLDPMLAPALADDTRAALDDAAALLESLGHHVEPATLQVNYARAAETFLTLWATIAEEMVLGARA
LTGRTPKRGEFEAATWAMAVVGRRLARTRLPDVLEWQRQLTVQVAGLVSRYDAILCASLAGPPVKIGELQPTPFETTQMK
VLAALPVKPLLKEMLAKSSEKAFTWAGCTELFNLTGQPAMSVPLYWNARGLPIGVQFAARHADDALLLRLARQLEQARPW
FERRPPLMQAQR
>Mature_492_residues
MHSDYLAHDAIGLATLVRERKASPRELLDAAIGQAEAVNSAINAIVLQDYEAARQRAAATPDGGADAPFAGVPYLVKDLG
AAVAGLPLSMGSRHYRYFVPADDSPVIARSRAAGLNVFGKTNTSEIGQMPYTEPELFGACRNPWNLDHTPGGSSGGAAAA
VAAGIVPIAHASDGGGSIRIPASCCGLFGLKPSRNPVLVDLPSNGELVVQHAVTRSVRDSALLLDVTTGQTLPAGAPGTF
LGELDTPPGPLRIGLVLDPMLAPALADDTRAALDDAAALLESLGHHVEPATLQVNYARAAETFLTLWATIAEEMVLGARA
LTGRTPKRGEFEAATWAMAVVGRRLARTRLPDVLEWQRQLTVQVAGLVSRYDAILCASLAGPPVKIGELQPTPFETTQMK
VLAALPVKPLLKEMLAKSSEKAFTWAGCTELFNLTGQPAMSVPLYWNARGLPIGVQFAARHADDALLLRLARQLEQARPW
FERRPPLMQAQR

Specific function: Specifically catalyzes the hydrolysis of 6-aminohexanoic acid cyclic dimer (1,8-diazacyclotetradecane-2,9-dione) to form the linear dimer 6-aminohexanoyl-6-aminohexanoic acid. Is inactive on 6-aminohexanoic acid oligomers (degree of polymerization 2 to 6)

COG id: COG0154

COG function: function code J; Asp-tRNAAsn/Glu-tRNAGln amidotransferase A subunit and related amidases

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the amidase family [H]

Homologues:

Organism=Homo sapiens, GI195972892, Length=206, Percent_Identity=35.4368932038835, Blast_Score=108, Evalue=1e-23,
Organism=Homo sapiens, GI222831590, Length=524, Percent_Identity=25.5725190839695, Blast_Score=86, Evalue=8e-17,
Organism=Caenorhabditis elegans, GI17537465, Length=505, Percent_Identity=24.1584158415842, Blast_Score=98, Evalue=1e-20,
Organism=Caenorhabditis elegans, GI17556264, Length=298, Percent_Identity=29.1946308724832, Blast_Score=83, Evalue=4e-16,
Organism=Caenorhabditis elegans, GI17556276, Length=317, Percent_Identity=26.4984227129338, Blast_Score=79, Evalue=5e-15,
Organism=Caenorhabditis elegans, GI17556278, Length=316, Percent_Identity=26.5822784810127, Blast_Score=78, Evalue=9e-15,
Organism=Caenorhabditis elegans, GI17543272, Length=473, Percent_Identity=25.1585623678647, Blast_Score=75, Evalue=8e-14,
Organism=Caenorhabditis elegans, GI32565337, Length=258, Percent_Identity=29.0697674418605, Blast_Score=67, Evalue=1e-11,
Organism=Caenorhabditis elegans, GI17538252, Length=175, Percent_Identity=31.4285714285714, Blast_Score=67, Evalue=2e-11,
Organism=Drosophila melanogaster, GI45550774, Length=504, Percent_Identity=25.5952380952381, Blast_Score=110, Evalue=2e-24,
Organism=Drosophila melanogaster, GI24648435, Length=504, Percent_Identity=25.5952380952381, Blast_Score=110, Evalue=2e-24,
Organism=Drosophila melanogaster, GI24648437, Length=504, Percent_Identity=25.5952380952381, Blast_Score=110, Evalue=2e-24,
Organism=Drosophila melanogaster, GI24644968, Length=511, Percent_Identity=25.2446183953033, Blast_Score=106, Evalue=4e-23,
Organism=Drosophila melanogaster, GI21356731, Length=203, Percent_Identity=31.5270935960591, Blast_Score=94, Evalue=2e-19,
Organism=Drosophila melanogaster, GI24648441, Length=427, Percent_Identity=24.824355971897, Blast_Score=86, Evalue=5e-17,
Organism=Drosophila melanogaster, GI24648439, Length=427, Percent_Identity=24.824355971897, Blast_Score=86, Evalue=5e-17,
Organism=Drosophila melanogaster, GI161078093, Length=234, Percent_Identity=31.1965811965812, Blast_Score=81, Evalue=2e-15,
Organism=Drosophila melanogaster, GI24652985, Length=200, Percent_Identity=31.5, Blast_Score=79, Evalue=5e-15,
Organism=Drosophila melanogaster, GI19922090, Length=200, Percent_Identity=31.5, Blast_Score=79, Evalue=5e-15,
Organism=Drosophila melanogaster, GI24652981, Length=200, Percent_Identity=31.5, Blast_Score=79, Evalue=5e-15,
Organism=Drosophila melanogaster, GI24652983, Length=200, Percent_Identity=31.5, Blast_Score=79, Evalue=5e-15,
Organism=Drosophila melanogaster, GI24648113, Length=482, Percent_Identity=28.4232365145228, Blast_Score=79, Evalue=7e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000120
- InterPro:   IPR020556 [H]

Pfam domain/function: PF01425 Amidase [H]

EC number: =3.5.2.12 [H]

Molecular weight: Translated: 52187; Mature: 52187

Theoretical pI: Translated: 6.79; Mature: 6.79

Prosite motif: PS00571 AMIDASES

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MHSDYLAHDAIGLATLVRERKASPRELLDAAIGQAEAVNSAINAIVLQDYEAARQRAAAT
CCCCHHHHHHHHHHHHHHHHCCCHHHHHHHHHCHHHHHHHHHHHHEECCHHHHHHHCCCC
PDGGADAPFAGVPYLVKDLGAAVAGLPLSMGSRHYRYFVPADDSPVIARSRAAGLNVFGK
CCCCCCCCCCCCHHHHHHHHHHHHCCCCCCCCCEEEEEECCCCCCCEEHHCCCCCEEECC
TNTSEIGQMPYTEPELFGACRNPWNLDHTPGGSSGGAAAAVAAGIVPIAHASDGGGSIRI
CCCHHHCCCCCCCHHHHHHCCCCCCCCCCCCCCCCCHHHHHHHCCEEEEECCCCCCEEEE
PASCCGLFGLKPSRNPVLVDLPSNGELVVQHAVTRSVRDSALLLDVTTGQTLPAGAPGTF
CHHHHHHCCCCCCCCCEEEECCCCCCEEEHHHHHHHHCCCEEEEEEECCCCCCCCCCCCE
LGELDTPPGPLRIGLVLDPMLAPALADDTRAALDDAAALLESLGHHVEPATLQVNYARAA
ECCCCCCCCCEEEEEEECCHHHHHHCCHHHHHHHHHHHHHHHHCCCCCCCEEEEEHHHHH
ETFLTLWATIAEEMVLGARALTGRTPKRGEFEAATWAMAVVGRRLARTRLPDVLEWQRQL
HHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCHHHHHHHHH
TVQVAGLVSRYDAILCASLAGPPVKIGELQPTPFETTQMKVLAALPVKPLLKEMLAKSSE
HHHHHHHHHHHHHHHHHHCCCCCEEECCCCCCCCCHHHHHHHHHCCHHHHHHHHHHCCCC
KAFTWAGCTELFNLTGQPAMSVPLYWNARGLPIGVQFAARHADDALLLRLARQLEQARPW
CCEEEHHHHHHHCCCCCCCEECEEEECCCCCCEEHHHHHHCCCHHHHHHHHHHHHHHCCH
FERRPPLMQAQR
HHHCCCCCCCCC
>Mature Secondary Structure
MHSDYLAHDAIGLATLVRERKASPRELLDAAIGQAEAVNSAINAIVLQDYEAARQRAAAT
CCCCHHHHHHHHHHHHHHHHCCCHHHHHHHHHCHHHHHHHHHHHHEECCHHHHHHHCCCC
PDGGADAPFAGVPYLVKDLGAAVAGLPLSMGSRHYRYFVPADDSPVIARSRAAGLNVFGK
CCCCCCCCCCCCHHHHHHHHHHHHCCCCCCCCCEEEEEECCCCCCCEEHHCCCCCEEECC
TNTSEIGQMPYTEPELFGACRNPWNLDHTPGGSSGGAAAAVAAGIVPIAHASDGGGSIRI
CCCHHHCCCCCCCHHHHHHCCCCCCCCCCCCCCCCCHHHHHHHCCEEEEECCCCCCEEEE
PASCCGLFGLKPSRNPVLVDLPSNGELVVQHAVTRSVRDSALLLDVTTGQTLPAGAPGTF
CHHHHHHCCCCCCCCCEEEECCCCCCEEEHHHHHHHHCCCEEEEEEECCCCCCCCCCCCE
LGELDTPPGPLRIGLVLDPMLAPALADDTRAALDDAAALLESLGHHVEPATLQVNYARAA
ECCCCCCCCCEEEEEEECCHHHHHHCCHHHHHHHHHHHHHHHHCCCCCCCEEEEEHHHHH
ETFLTLWATIAEEMVLGARALTGRTPKRGEFEAATWAMAVVGRRLARTRLPDVLEWQRQL
HHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHCCCHHHHHHHHH
TVQVAGLVSRYDAILCASLAGPPVKIGELQPTPFETTQMKVLAALPVKPLLKEMLAKSSE
HHHHHHHHHHHHHHHHHHCCCCCEEECCCCCCCCCHHHHHHHHHCCHHHHHHHHHHCCCC
KAFTWAGCTELFNLTGQPAMSVPLYWNARGLPIGVQFAARHADDALLLRLARQLEQARPW
CCEEEHHHHHHHCCCCCCCEECEEEECCCCCCEEHHHHHHCCCHHHHHHHHHHHHHHCCH
FERRPPLMQAQR
HHHCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 2722746 [H]