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The map label for this gene is tfdEI [H]

Identifier: 78064856

GI number: 78064856

Start: 238366

End: 239058

Strand: Reverse

Name: tfdEI [H]

Synonym: Bcep18194_A3379

Alternate gene names: 78064856

Gene position: 239058-238366 (Counterclockwise)

Preceding gene: 78064859

Following gene: 78064855

Centisome position: 6.47

GC content: 69.26

Gene sequence:

>693_bases
GTGACTGCCCAATGGATCGACATCCCCGCCGGTAACGACAGCTTCGGCGGCTATCTCGCGCTGCCCAAGCGCGGCACCGG
CCCCGCCGTCATCATCATCCAGGAGATCTTCGGCGTGAACTCGCACATCCGCGCGGTCGCCGACCAGTACGCGTCCGACG
GCTTCGTCGCGCTCGCGCCGGACGTGTTCTGGCGCACGCAGCCGCGCGTCGAGCTGACCTACGAAGGCGCGGATCGCGAC
AAGGGCATCGAGCTGATGAAAAAGACCGACGTGGGTCTCGCGGTCGCGGACATCGGCGCGGCAGCCGATGCACTGCGCGC
ACGCCCCGAAGTGGCCGGCAAGGTCGCCGCGATCGGCTACTGCTTCGGCGGCCAGCTCGCGTACCGCGCGGCGGCGACCG
GCAAGATCGACGCGGCCGTGGCCTACTACGGCGGCGGCATCCAGAACGCGCTCGACCTCGCCGGCAAGGTCACGCAGCCG
ATCCTGTTCCACTACGCGGAAAACGACCACGGCATCCCGCTCACGGCCGTCGACCAGGTGAAGGCCGCATTCGCCGGCCA
CGACCATGCGTCGTTCCACGTGTACCCGGGCGCCGAGCACGGCTTCAACTGCACGGACCGCGCGTCGTACAACCAGCGCG
CGTCGGCGCTCGCGCATGGCCGCACGCTGACCTTCCTCGCCGAGCACCTCTAA

Upstream 100 bases:

>100_bases
GGCGCGTGCCCTGCACCGTCACACGATCGGTGGCACACTAGGCCGCCCTCTTTCACCCCGGGGCGCGCATCGCGCACCCC
GTTCGCCAGGAGTTCAGAAC

Downstream 100 bases:

>100_bases
AAACGGCCGGCCGCGGGCCCCGCGCACCGGGCCCGCGTTATGCTGCTGTCATCGCCACGGGTCACAACGGGGGTGACTGC
GATGCACTCGGACTATCTCG

Product: carboxymethylenebutenolidase

Products: NA

Alternate protein names: Carboxymethylenebutenolidase I; Dienelactone hydrolase I; DLH I [H]

Number of amino acids: Translated: 230; Mature: 229

Protein sequence:

>230_residues
MTAQWIDIPAGNDSFGGYLALPKRGTGPAVIIIQEIFGVNSHIRAVADQYASDGFVALAPDVFWRTQPRVELTYEGADRD
KGIELMKKTDVGLAVADIGAAADALRARPEVAGKVAAIGYCFGGQLAYRAAATGKIDAAVAYYGGGIQNALDLAGKVTQP
ILFHYAENDHGIPLTAVDQVKAAFAGHDHASFHVYPGAEHGFNCTDRASYNQRASALAHGRTLTFLAEHL

Sequences:

>Translated_230_residues
MTAQWIDIPAGNDSFGGYLALPKRGTGPAVIIIQEIFGVNSHIRAVADQYASDGFVALAPDVFWRTQPRVELTYEGADRD
KGIELMKKTDVGLAVADIGAAADALRARPEVAGKVAAIGYCFGGQLAYRAAATGKIDAAVAYYGGGIQNALDLAGKVTQP
ILFHYAENDHGIPLTAVDQVKAAFAGHDHASFHVYPGAEHGFNCTDRASYNQRASALAHGRTLTFLAEHL
>Mature_229_residues
TAQWIDIPAGNDSFGGYLALPKRGTGPAVIIIQEIFGVNSHIRAVADQYASDGFVALAPDVFWRTQPRVELTYEGADRDK
GIELMKKTDVGLAVADIGAAADALRARPEVAGKVAAIGYCFGGQLAYRAAATGKIDAAVAYYGGGIQNALDLAGKVTQPI
LFHYAENDHGIPLTAVDQVKAAFAGHDHASFHVYPGAEHGFNCTDRASYNQRASALAHGRTLTFLAEHL

Specific function: Ring cleavage of cyclic ester dienelactone to produce maleylacetate [H]

COG id: COG0412

COG function: function code Q; Dienelactone hydrolase and related enzymes

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the dienelactone hydrolase family [H]

Homologues:

Organism=Escherichia coli, GI48994982, Length=242, Percent_Identity=29.3388429752066, Blast_Score=97, Evalue=7e-22,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002925 [H]

Pfam domain/function: PF01738 DLH [H]

EC number: =3.1.1.45 [H]

Molecular weight: Translated: 24384; Mature: 24252

Theoretical pI: Translated: 6.42; Mature: 6.42

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
0.9 %Met     (Translated Protein)
1.7 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
0.4 %Met     (Mature Protein)
1.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTAQWIDIPAGNDSFGGYLALPKRGTGPAVIIIQEIFGVNSHIRAVADQYASDGFVALAP
CCCEEEECCCCCCCCCCEEEECCCCCCCEEEEEEHHHCCCHHHHHHHHHHCCCCEEEECC
DVFWRTQPRVELTYEGADRDKGIELMKKTDVGLAVADIGAAADALRARPEVAGKVAAIGY
CHHCCCCCCEEEEECCCCCCCCHHEEECCCCCEEEEHHHHHHHHHHCCHHHHCCEEEEEE
CFGGQLAYRAAATGKIDAAVAYYGGGIQNALDLAGKVTQPILFHYAENDHGIPLTAVDQV
EECCCEEEEEECCCCCEEEEEEECCCHHHHHHHHHHHCCCEEEEECCCCCCCCEEHHHHH
KAAFAGHDHASFHVYPGAEHGFNCTDRASYNQRASALAHGRTLTFLAEHL
HHHHCCCCCCEEEEECCCCCCCCCCCCCCCHHHHHHHHCCCHHHHHHHCC
>Mature Secondary Structure 
TAQWIDIPAGNDSFGGYLALPKRGTGPAVIIIQEIFGVNSHIRAVADQYASDGFVALAP
CCEEEECCCCCCCCCCEEEECCCCCCCEEEEEEHHHCCCHHHHHHHHHHCCCCEEEECC
DVFWRTQPRVELTYEGADRDKGIELMKKTDVGLAVADIGAAADALRARPEVAGKVAAIGY
CHHCCCCCCEEEEECCCCCCCCHHEEECCCCCEEEEHHHHHHHHHHCCHHHHCCEEEEEE
CFGGQLAYRAAATGKIDAAVAYYGGGIQNALDLAGKVTQPILFHYAENDHGIPLTAVDQV
EECCCEEEEEECCCCCEEEEEEECCCHHHHHHHHHHHCCCEEEEECCCCCCCCEEHHHHH
KAAFAGHDHASFHVYPGAEHGFNCTDRASYNQRASALAHGRTLTFLAEHL
HHHHCCCCCCEEEEECCCCCCCCCCCCCCCHHHHHHHHCCCHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 2185214 [H]