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The map label for this gene is flhF

Identifier: 78064849

GI number: 78064849

Start: 230348

End: 232111

Strand: Direct

Name: flhF

Synonym: Bcep18194_A3372

Alternate gene names: 78064849

Gene position: 230348-232111 (Clockwise)

Preceding gene: 78064848

Following gene: 78064850

Centisome position: 6.24

GC content: 70.46

Gene sequence:

>1764_bases
TTGAACATTCGCAAATTCACCGGCGCAACGAGCCGCGACGCACTTCGTCTCGTGCGCGAGGCACTCGGCGCCGACGCGGT
CGTGCTGTCGAACCGCACGCTCGATGACGGCAGCGTGGAAATCGTCGCACTGGCAGATTCGGACCTGGCCGCCGTGGCGC
CGCCGGCTGCGGTGCGCCCGCGCATCGCTGCGCCGCGCATGCCGGAATCCGTGCCGGCCGCCGCCGTGCCGGGCATCGTG
TCGCGCCCGGGTGCGGCGCTGGCCCGTCCGGCGGTCAATCCGTATGCAGCAGGCGAAGGCGGGCTGCCGGACGTGTTCTC
GTCGGTGTTCGGCGCAAGCGCCGAAGCAGACGACACGGTGGCGCAACCTTCGGCGATCGATGACGCTGCGCTGGCCGCGG
CGGCGCCGTCTTCCGCCGCCGCCCCGACTGCCGCCGCCCCGGCCGCTGCTTCCGAACCCGCACCGTGGCTGGTCGAGCAC
GCGAAGCGCCTGACGCAGCAGCGCGATGCGCTGATGGCGCGTGCGCAGGCAACGCCCGCCGCGCCGCAGGCGAGCGTGCC
GGCCCCGCAGGCCGCCGCGAGCGCGACGCCGCCCGAGTGGGCACGCGACATCGTGCGCGACGCCGAACGCCGGATGCCGG
CCGCCGCCACCCGCGCGCCCGACACGAACGCGAACGCGAACGCCGCGTATGCCGCGAAGGCGGCCGAGCGCACGCGCGTG
TCCGCCGACGCCGCCGCCGCGGTGGCCGACGCCGTGAAGTCGCGCATCGAGCGGATCGTCAACGATACGGTGATGCAGGA
GCTCGGCGAGCTGCGCGGGATGATGGAAGAGCAGTTCGACAGCCTGATGTGGCACGACCGCCAGCGTCGCAGCGCCGTGC
ATGGCGCGCTGACGAAGCACCTGTTCGCGGCCGGTTTCTCCGCGCAGCTCGTGCGGATGCTGGTCGACAACATGCCGGCC
GGCGACGGCGCGCAGACCTTCGAACAGGCCACCGAATGGGCGCAGTCGGTGCTTGCGTCGAACCTGCCGGTGCTCGAAAG
TGAAGATGCGCTGATGGAGCGCGGCGGCGTGTTCGCGCTGATGGGGCCGACGGGCGTCGGCAAGACGACCACCACCGCGA
AGCTCGCTGCGCGCTGCGTGATGCGCTTCGGCGCGAGCAAGGTCGCGCTGCTGACCACCGACAGCTACCGGATCGGCGGC
CACGAGCAGCTGCGCATTTTCGGCAAGATCCTCGGCGTGCCCGTGCACGCGGTGAAGGATGCGGGCGACCTCGCACTGGC
GCTGTCCGAGCTGCGCAACAAGCACATCGTGCTGATCGACACGATCGGCATGAGCCAGCGCGACCGCGCGGTGTCCGACC
AGATCGCGATGCTGCACGGTGCGAACGCGCCGGTTCAGCGCCTGCTGCTGCTCAACGCGACGAGCCACGGCGACACGCTC
AACGAAGTCGTGCAGGCGTACCGCAGCGCGGGCGAGCATCCGGACCTGGCCGGCTGCATCCTCACGAAGCTCGACGAGGC
GACCCACCTCGGCGGCGTGCTCGACACGGTGATCCGCTACAAGCTGCCGATCCATTACGTGTCGACCGGCCAGAAGGTGC
CGGAGAACCTGTACGTCGCCTCGACCAAATTCCTGCTGAAGAGCGCGTTCTGTGTACCGCGCGACGGCTCGCCCTTCGTG
CCGCAAGACGAGGACATGCCGACGCTGCTTTCCGCACTGACCGCACGTTCCACCGCCGAGCTGCACGAGGTGCGATTTGG
ATAA

Upstream 100 bases:

>100_bases
GTTTCCTGCGCCGCAGCCTGCCGCAATTGAAAGTGCTGTCGTATGCCGAAGTGCCGGACACACGCACGATCAAAGTCGTT
AACGTCATCGGGGGTTCCGC

Downstream 100 bases:

>100_bases
ACGAATCATCGATCAGGCCGAAGGGCTGCGGCGCCTGCTGGCCGGGCGCGCATCGCGCATCGTCGCGGTGACGGGCGGGC
CGGCGGGTGTCGGCTGCACG

Product: flagellar biosynthesis regulator FlhF

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 587; Mature: 587

Protein sequence:

>587_residues
MNIRKFTGATSRDALRLVREALGADAVVLSNRTLDDGSVEIVALADSDLAAVAPPAAVRPRIAAPRMPESVPAAAVPGIV
SRPGAALARPAVNPYAAGEGGLPDVFSSVFGASAEADDTVAQPSAIDDAALAAAAPSSAAAPTAAAPAAASEPAPWLVEH
AKRLTQQRDALMARAQATPAAPQASVPAPQAAASATPPEWARDIVRDAERRMPAAATRAPDTNANANAAYAAKAAERTRV
SADAAAAVADAVKSRIERIVNDTVMQELGELRGMMEEQFDSLMWHDRQRRSAVHGALTKHLFAAGFSAQLVRMLVDNMPA
GDGAQTFEQATEWAQSVLASNLPVLESEDALMERGGVFALMGPTGVGKTTTTAKLAARCVMRFGASKVALLTTDSYRIGG
HEQLRIFGKILGVPVHAVKDAGDLALALSELRNKHIVLIDTIGMSQRDRAVSDQIAMLHGANAPVQRLLLLNATSHGDTL
NEVVQAYRSAGEHPDLAGCILTKLDEATHLGGVLDTVIRYKLPIHYVSTGQKVPENLYVASTKFLLKSAFCVPRDGSPFV
PQDEDMPTLLSALTARSTAELHEVRFG

Sequences:

>Translated_587_residues
MNIRKFTGATSRDALRLVREALGADAVVLSNRTLDDGSVEIVALADSDLAAVAPPAAVRPRIAAPRMPESVPAAAVPGIV
SRPGAALARPAVNPYAAGEGGLPDVFSSVFGASAEADDTVAQPSAIDDAALAAAAPSSAAAPTAAAPAAASEPAPWLVEH
AKRLTQQRDALMARAQATPAAPQASVPAPQAAASATPPEWARDIVRDAERRMPAAATRAPDTNANANAAYAAKAAERTRV
SADAAAAVADAVKSRIERIVNDTVMQELGELRGMMEEQFDSLMWHDRQRRSAVHGALTKHLFAAGFSAQLVRMLVDNMPA
GDGAQTFEQATEWAQSVLASNLPVLESEDALMERGGVFALMGPTGVGKTTTTAKLAARCVMRFGASKVALLTTDSYRIGG
HEQLRIFGKILGVPVHAVKDAGDLALALSELRNKHIVLIDTIGMSQRDRAVSDQIAMLHGANAPVQRLLLLNATSHGDTL
NEVVQAYRSAGEHPDLAGCILTKLDEATHLGGVLDTVIRYKLPIHYVSTGQKVPENLYVASTKFLLKSAFCVPRDGSPFV
PQDEDMPTLLSALTARSTAELHEVRFG
>Mature_587_residues
MNIRKFTGATSRDALRLVREALGADAVVLSNRTLDDGSVEIVALADSDLAAVAPPAAVRPRIAAPRMPESVPAAAVPGIV
SRPGAALARPAVNPYAAGEGGLPDVFSSVFGASAEADDTVAQPSAIDDAALAAAAPSSAAAPTAAAPAAASEPAPWLVEH
AKRLTQQRDALMARAQATPAAPQASVPAPQAAASATPPEWARDIVRDAERRMPAAATRAPDTNANANAAYAAKAAERTRV
SADAAAAVADAVKSRIERIVNDTVMQELGELRGMMEEQFDSLMWHDRQRRSAVHGALTKHLFAAGFSAQLVRMLVDNMPA
GDGAQTFEQATEWAQSVLASNLPVLESEDALMERGGVFALMGPTGVGKTTTTAKLAARCVMRFGASKVALLTTDSYRIGG
HEQLRIFGKILGVPVHAVKDAGDLALALSELRNKHIVLIDTIGMSQRDRAVSDQIAMLHGANAPVQRLLLLNATSHGDTL
NEVVQAYRSAGEHPDLAGCILTKLDEATHLGGVLDTVIRYKLPIHYVSTGQKVPENLYVASTKFLLKSAFCVPRDGSPFV
PQDEDMPTLLSALTARSTAELHEVRFG

Specific function: Necessary for flagellar biosynthesis. May be involved in translocation of the flagellum [H]

COG id: COG1419

COG function: function code N; Flagellar GTP-binding protein

Gene ontology:

Cell location: Cell membrane; Peripheral membrane protein; Cytoplasmic side [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the GTP-binding SRP family [H]

Homologues:

Organism=Escherichia coli, GI1789874, Length=190, Percent_Identity=28.9473684210526, Blast_Score=70, Evalue=5e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003593
- InterPro:   IPR000897
- InterPro:   IPR020006 [H]

Pfam domain/function: PF00448 SRP54 [H]

EC number: NA

Molecular weight: Translated: 61617; Mature: 61617

Theoretical pI: Translated: 6.33; Mature: 6.33

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNIRKFTGATSRDALRLVREALGADAVVLSNRTLDDGSVEIVALADSDLAAVAPPAAVRP
CCCCCCCCCCHHHHHHHHHHHHCCCEEEEECCCCCCCCEEEEEECCCCCHHCCCCHHCCC
RIAAPRMPESVPAAAVPGIVSRPGAALARPAVNPYAAGEGGLPDVFSSVFGASAEADDTV
CCCCCCCCCCCCCHHCCCCCCCCCHHHHCCCCCCCCCCCCCCHHHHHHHHCCCCCCCCCC
AQPSAIDDAALAAAAPSSAAAPTAAAPAAASEPAPWLVEHAKRLTQQRDALMARAQATPA
CCCCCCCHHHHHHCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCC
APQASVPAPQAAASATPPEWARDIVRDAERRMPAAATRAPDTNANANAAYAAKAAERTRV
CCCCCCCCCCHHCCCCCHHHHHHHHHHHHHCCCCHHCCCCCCCCCCCHHHHHHHHHHHHH
SADAAAAVADAVKSRIERIVNDTVMQELGELRGMMEEQFDSLMWHDRQRRSAVHGALTKH
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LFAAGFSAQLVRMLVDNMPAGDGAQTFEQATEWAQSVLASNLPVLESEDALMERGGVFAL
HHHHCHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHCCCEEEE
MGPTGVGKTTTTAKLAARCVMRFGASKVALLTTDSYRIGGHEQLRIFGKILGVPVHAVKD
ECCCCCCCCHHHHHHHHHHHHHHCCCEEEEEEECCEECCCHHHHHHHHHHHCCCHHHHHC
AGDLALALSELRNKHIVLIDTIGMSQRDRAVSDQIAMLHGANAPVQRLLLLNATSHGDTL
CHHHHHHHHHHCCCCEEEEEECCCCCHHHHHHHHHHHHHCCCCHHHHHHHHCCCCCCHHH
NEVVQAYRSAGEHPDLAGCILTKLDEATHLGGVLDTVIRYKLPIHYVSTGQKVPENLYVA
HHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCEEEECCCCCCCCHHHHH
STKFLLKSAFCVPRDGSPFVPQDEDMPTLLSALTARSTAELHEVRFG
HHHHHHHHHCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MNIRKFTGATSRDALRLVREALGADAVVLSNRTLDDGSVEIVALADSDLAAVAPPAAVRP
CCCCCCCCCCHHHHHHHHHHHHCCCEEEEECCCCCCCCEEEEEECCCCCHHCCCCHHCCC
RIAAPRMPESVPAAAVPGIVSRPGAALARPAVNPYAAGEGGLPDVFSSVFGASAEADDTV
CCCCCCCCCCCCCHHCCCCCCCCCHHHHCCCCCCCCCCCCCCHHHHHHHHCCCCCCCCCC
AQPSAIDDAALAAAAPSSAAAPTAAAPAAASEPAPWLVEHAKRLTQQRDALMARAQATPA
CCCCCCCHHHHHHCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCC
APQASVPAPQAAASATPPEWARDIVRDAERRMPAAATRAPDTNANANAAYAAKAAERTRV
CCCCCCCCCCHHCCCCCHHHHHHHHHHHHHCCCCHHCCCCCCCCCCCHHHHHHHHHHHHH
SADAAAAVADAVKSRIERIVNDTVMQELGELRGMMEEQFDSLMWHDRQRRSAVHGALTKH
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LFAAGFSAQLVRMLVDNMPAGDGAQTFEQATEWAQSVLASNLPVLESEDALMERGGVFAL
HHHHCHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHCCCEEEE
MGPTGVGKTTTTAKLAARCVMRFGASKVALLTTDSYRIGGHEQLRIFGKILGVPVHAVKD
ECCCCCCCCHHHHHHHHHHHHHHCCCEEEEEEECCEECCCHHHHHHHHHHHCCCHHHHHC
AGDLALALSELRNKHIVLIDTIGMSQRDRAVSDQIAMLHGANAPVQRLLLLNATSHGDTL
CHHHHHHHHHHCCCCEEEEEECCCCCHHHHHHHHHHHHHCCCCHHHHHHHHCCCCCCHHH
NEVVQAYRSAGEHPDLAGCILTKLDEATHLGGVLDTVIRYKLPIHYVSTGQKVPENLYVA
HHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCEEEECCCCCCCCHHHHH
STKFLLKSAFCVPRDGSPFVPQDEDMPTLLSALTARSTAELHEVRFG
HHHHHHHHHCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 9503621 [H]