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The map label for this gene is rob [H]

Identifier: 78064783

GI number: 78064783

Start: 148205

End: 149083

Strand: Reverse

Name: rob [H]

Synonym: Bcep18194_A3306

Alternate gene names: 78064783

Gene position: 149083-148205 (Counterclockwise)

Preceding gene: 78064788

Following gene: 78064780

Centisome position: 4.04

GC content: 66.67

Gene sequence:

>879_bases
ATGACCGCCGCAACGAAACCCGACCAGCCCGCCTGGCTTCGCTATGCGGCGAGCGTGGCGCGCGTCCACGAGTACATCCA
CGCGCACCTCGACGGCCCCCTCGACCTGAACCGGCTCGCCGAAGTCGCGTGCCTGTCGCCGTATCACTGGCAGCGCGTCT
ATCGCGCGCTGTATGGCGAGTCGATCGTGCTGACGGTGCGGCGGATGCGGATCGTGCGCGCGTCCGAGGATCTCGTGTGC
ACGGCGCGGCCGATCGACGAAATCGCGCGGCGCGCAGGCTACGGTTCGACGCACGCGTTCGCGCGGGCGTTTCGCGACGC
ATACGGCGTGCCTCCCGTGCAGCATCGGCGCACGGGCAGCCACCGGCCCATCTATCCGTTACAGCGAGGAGAAGAAGACA
TGTTCAAGCATGAAGTCGAGATCCGGCAGCTACCGGAATTACACGGCTATGCAGTGGCGCACACCGGTTCGTACTTGAAG
GTCAGCGAAGCGTTCATGCGAATCGGCGCGTGGGTCGGGCAGCACGGGTTGATCGGCCCCGGCGCGAAGATGATCGGCAT
TTTTTACGACGATCCGGACACGACACCCGAAGCGCAGTTGCGCGCGAAGGCCTGCTTCATGCCGGCCGACGGTGCGCCTG
ACGTCGAATCCGCGCCGCCGGTGGAGCACGCGACGGTAGCCGGCGGCGAATACGCGGTGCTGCTGCATACGGGGCCGTAT
GCGGACCTGAAGACGGCGTACCAGTGGCTCTATGGCGACTGGCTGCGTCACTCGGGTCGCGAAGCCGCCGACGCACCGCC
GTTCGAGCTGTACCTCAATACGCCGATGGATGCGGCGCCAGCCGACCTGCGCACCGAGATCTACCTGCCGCTGCGCTGA

Upstream 100 bases:

>100_bases
AATAAAAAAGGGCCTGATTTGTCGGCCTCCTACGCATTTCCGCAAGATTTATCCATTCGGCCCGTGCTACCGTGACGTCG
GTTCCCGCCCGGACACGCCG

Downstream 100 bases:

>100_bases
TCGACAGGCGGTACGCCGCTCCCTGAAACGAATCGCGCCGGCGGGCGTTCACGCGCCGCCGGCGCGAATGCGCGGACAAA
CTGCCTTACGCAGCCTGCGT

Product: AraC family transcriptional regulator

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 292; Mature: 291

Protein sequence:

>292_residues
MTAATKPDQPAWLRYAASVARVHEYIHAHLDGPLDLNRLAEVACLSPYHWQRVYRALYGESIVLTVRRMRIVRASEDLVC
TARPIDEIARRAGYGSTHAFARAFRDAYGVPPVQHRRTGSHRPIYPLQRGEEDMFKHEVEIRQLPELHGYAVAHTGSYLK
VSEAFMRIGAWVGQHGLIGPGAKMIGIFYDDPDTTPEAQLRAKACFMPADGAPDVESAPPVEHATVAGGEYAVLLHTGPY
ADLKTAYQWLYGDWLRHSGREAADAPPFELYLNTPMDAAPADLRTEIYLPLR

Sequences:

>Translated_292_residues
MTAATKPDQPAWLRYAASVARVHEYIHAHLDGPLDLNRLAEVACLSPYHWQRVYRALYGESIVLTVRRMRIVRASEDLVC
TARPIDEIARRAGYGSTHAFARAFRDAYGVPPVQHRRTGSHRPIYPLQRGEEDMFKHEVEIRQLPELHGYAVAHTGSYLK
VSEAFMRIGAWVGQHGLIGPGAKMIGIFYDDPDTTPEAQLRAKACFMPADGAPDVESAPPVEHATVAGGEYAVLLHTGPY
ADLKTAYQWLYGDWLRHSGREAADAPPFELYLNTPMDAAPADLRTEIYLPLR
>Mature_291_residues
TAATKPDQPAWLRYAASVARVHEYIHAHLDGPLDLNRLAEVACLSPYHWQRVYRALYGESIVLTVRRMRIVRASEDLVCT
ARPIDEIARRAGYGSTHAFARAFRDAYGVPPVQHRRTGSHRPIYPLQRGEEDMFKHEVEIRQLPELHGYAVAHTGSYLKV
SEAFMRIGAWVGQHGLIGPGAKMIGIFYDDPDTTPEAQLRAKACFMPADGAPDVESAPPVEHATVAGGEYAVLLHTGPYA
DLKTAYQWLYGDWLRHSGREAADAPPFELYLNTPMDAAPADLRTEIYLPLR

Specific function: Binds to the right arm of the replication origin oriC of the chromosome. Rob binding may influence the formation of the nucleoprotein structure, required for oriC function in the initiation of replication [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH araC/xylS-type DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1790857, Length=284, Percent_Identity=26.056338028169, Blast_Score=79, Evalue=4e-16,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR010499
- InterPro:   IPR009057
- InterPro:   IPR012287
- InterPro:   IPR018062
- InterPro:   IPR020449
- InterPro:   IPR018060
- InterPro:   IPR011256 [H]

Pfam domain/function: PF06445 AraC_E_bind; PF00165 HTH_AraC [H]

EC number: NA

Molecular weight: Translated: 32641; Mature: 32510

Theoretical pI: Translated: 6.89; Mature: 6.89

Prosite motif: PS00041 HTH_ARAC_FAMILY_1 ; PS01124 HTH_ARAC_FAMILY_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTAATKPDQPAWLRYAASVARVHEYIHAHLDGPLDLNRLAEVACLSPYHWQRVYRALYGE
CCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHCCCHHHHHHHHHHHCC
SIVLTVRRMRIVRASEDLVCTARPIDEIARRAGYGSTHAFARAFRDAYGVPPVQHRRTGS
HHEEHHHHHHHHHCCCCEEEECCCHHHHHHHCCCCCHHHHHHHHHHHCCCCCCHHCCCCC
HRPIYPLQRGEEDMFKHEVEIRQLPELHGYAVAHTGSYLKVSEAFMRIGAWVGQHGLIGP
CCCCCCCCCCCHHHHHHHHHHHHCCHHCCEEEEECCCHHHHHHHHHHHHHHHCCCCCCCC
GAKMIGIFYDDPDTTPEAQLRAKACFMPADGAPDVESAPPVEHATVAGGEYAVLLHTGPY
CCEEEEEEECCCCCCHHHHHHHHEEECCCCCCCCCCCCCCCCCEEECCCCEEEEEECCCC
ADLKTAYQWLYGDWLRHSGREAADAPPFELYLNTPMDAAPADLRTEIYLPLR
HHHHHHHHHHHHHHHHHCCCCCCCCCCEEEEECCCCCCCCCCCCEEEEECCC
>Mature Secondary Structure 
TAATKPDQPAWLRYAASVARVHEYIHAHLDGPLDLNRLAEVACLSPYHWQRVYRALYGE
CCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHCCCHHHHHHHHHHHCC
SIVLTVRRMRIVRASEDLVCTARPIDEIARRAGYGSTHAFARAFRDAYGVPPVQHRRTGS
HHEEHHHHHHHHHCCCCEEEECCCHHHHHHHCCCCCHHHHHHHHHHHCCCCCCHHCCCCC
HRPIYPLQRGEEDMFKHEVEIRQLPELHGYAVAHTGSYLKVSEAFMRIGAWVGQHGLIGP
CCCCCCCCCCCHHHHHHHHHHHHCCHHCCEEEEECCCHHHHHHHHHHHHHHHCCCCCCCC
GAKMIGIFYDDPDTTPEAQLRAKACFMPADGAPDVESAPPVEHATVAGGEYAVLLHTGPY
CCEEEEEEECCCCCCHHHHHHHHEEECCCCCCCCCCCCCCCCCEEECCCCEEEEEECCCC
ADLKTAYQWLYGDWLRHSGREAADAPPFELYLNTPMDAAPADLRTEIYLPLR
HHHHHHHHHHHHHHHHHCCCCCCCCCCEEEEECCCCCCCCCCCCEEEEECCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]