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The map label for this gene is priA [H]

Identifier: 78064770

GI number: 78064770

Start: 128342

End: 130603

Strand: Direct

Name: priA [H]

Synonym: Bcep18194_A3293

Alternate gene names: 78064770

Gene position: 128342-130603 (Clockwise)

Preceding gene: 78064769

Following gene: 78064771

Centisome position: 3.47

GC content: 72.24

Gene sequence:

>2262_bases
ATGAGCGGCACCTATCTGCGCGTCGCGCTCGACCATCCGCTCGCCACCCTGTTCGATTACCGCTGCGACGCGCAACCGGT
GCCCGGGCCCGGCACGCTCGTGCAGGTGCCGTTCGGCAAGCGGCAGGCCGTCGGGCTCGTCTGCGAAGTGACGACTCACA
CCGATGTGCCGCCATCCCGGCTGCGCGCAGTCGACGCGATCTGCACCGACCTGCCGCCGTTGTCGCCGGACTGGCTCGCG
CTCGTGTCGTTCGCCGCCGACTACTACCAGCGCGGCCGTGGCGAGGTCGCGCTGCCGGCGCTGCCGCAGGCGCTGCGCGA
TGCCGGCCGCTGGGGGCGGCTGCTCGCGCCCGAGGTGCGCTACCGGCCGACGGAAGCCGGCCGCGCGGCGCTGCCCGATG
CATTGCCCGCGCGCGGGGCCGCGCTGCGGCGGCTCGCGCAGGCCCTGGTCGACACCGATTCGCTGGCGCTGCCCGATGCG
CGCGCGCTGCACCCGAAGGCGGCGGCGACGCTCGACGACTGGGCGGCGCGCGGCTGGGTCGACGTCGAGGAGATCGGCTG
GGCCGACGCGCCTGTGCCCAAAGCTGTGGATAACCTGTTGGCAGCCGGTGGACGAACTGTGCCGCCGGCGCTCACGGACC
AGCAGGCCGAGGCGCTCGACGCGATTCGCGCCGCACAGGGCTTCGCGCCGTTCCTGCTGCACGGCGTGACGGGCAGCGGC
AAGACCGAGGTCTATCTGCATGCGCTCGCATCGCTGCTCGATGCACGGCCGGACGCGCAAGCGCTCGTGCTCGTCCCCGA
AATCAACCTGACGCCGCAGTTCGAGGCCGCGTTTCGCGCGCGCTTCGCGGGGGCGCTCGCCGACGACGCGATCGTCACGC
TGCACAGCGGGCTCGCCGAGGGCGAGCGTGCGCGCAACTGGCTCGCCGCGCATACGGGCCGCGCGCGGATCGTGCTCGGC
ACGCGCCTCGCAGTGCTCGCGTCGATGCCGACACTCGCGTTGATCGTCGTCGACGAGGAGCACGAGCCCGCATACAAGCA
GCAGGAAGGGCTGCGCTATTCGGCACGCGATCTCGCCGTGTGGCGCGCGAAGCAGCTCGGCATCACGGTCGTGCTCGGCT
CGGCGACGCCGTCGCTCGAAAGCTGGTGGCAGGCCGAGCAGGGCCGTTACACGCGGCTCACGCTGTCGCGCCGCGCGGTG
GCTGACGCGACGCTGCCGACCGTGCGCCTGATCGACCTCGAAGAGGAGCGGCGGCGCGGGCGCGCGTCGATGGGCGGGCT
GTCGGGGCCGCTCGTCGCGGCGCTGAAGGCGCGGCTCGAGCGCGGCGAACAGAGCCTCGTGTTCCTGAACCGGCGCGGTT
ATGCGCCGCAGCTCGCGTGCGACGCATGCGGCTGGGTTGCCGGCTGCCCGCGCTGCAGCGCGTACGTCGTGCTGCACAAG
CCCGAGCACGCGCTGCGCTGCCATCACTGCGGCTGGGAATCGCGCATTCCGCGGTCGTGCCCCGAATGCGGGAACGTCGA
CATCGCGCCGCTCGGGCGCGGTACGCAACGGATCGAGGAGGCGCTCGCCGAGACCGTGCCGGGTGCGCGCGTGCTGCGGA
TCGACGCGGACAGCACACGTCGCAAGGGCAGTGCGCAGGCGCTCTTTTCCGACGTGCACGCGGGCGAGGTCGATATCCTC
GTCGGCACGCAGATGATCGCGAAGGGGCACGACTTCCAGCGCGTGTCACTCGTCGGCGTGCTCAATGCCGATACTGCGCT
GTTCTCGCACGACTTCCGCGCGAGCGAACGGCTGTTCGCGCAGCTGATGCAGGTGAGCGGCCGCGCAGGGCGCGCCGGGC
TGCCGGGCGAGGTGCTCGTGCAGACGCGTTACCCGCGTCATGCGCTGTACCACGCGCTCGGGCGGCAGGATTACGTCGGC
TTCGCGAATTCGACGCTTGGCGAGCGCCGCGACGCGCACCTGCCGCCGTTCGTCTACCAGGCGCTGCTGCGCGCCGAAGG
GCGCACGCTCGACGCGGCGCTCGCGTTCCTGCTGCAAGCCGCGGCCGCGTTGCCGGGGCTGCCGGGTGCCGATCGCGTGA
CCGTCTACGACGCGGTGCCGATGACGATCGTCAAGGTCGCGAACGTCCATCGCGCGCAGTTGCTGCTCGAAAGCGCGTCG
CGGGCGGCGCTGCAGCATGCGCTGCGCGCATGGCAGCCGGAGCTGCGCGCGCTGAAGGGCGTGCTGCGGTGGAGCGTCGA
GGTCGATCCGCTGGATATCTGA

Upstream 100 bases:

>100_bases
CGCCGAATCTGAAGTTTTACTTTAAGTTCGCTGCCACGATGCGGGCGCGGATGTCGGCCGTCCATGCCCATGCGGCGCAT
ACCGCCGCCGGAGCGCCGTG

Downstream 100 bases:

>100_bases
GTATCTGAGCGGCGCGCCATGCGGCGTTCGTGCTGCGCGCCGCGGTATCGAAGCCCGGGCCGGGTGCCCTCGAACCGTCT
CTCGCCGCTCGGCCGCGCCC

Product: primosome assembly protein PriA

Products: NA

Alternate protein names: ATP-dependent helicase priA; Replication factor Y [H]

Number of amino acids: Translated: 753; Mature: 752

Protein sequence:

>753_residues
MSGTYLRVALDHPLATLFDYRCDAQPVPGPGTLVQVPFGKRQAVGLVCEVTTHTDVPPSRLRAVDAICTDLPPLSPDWLA
LVSFAADYYQRGRGEVALPALPQALRDAGRWGRLLAPEVRYRPTEAGRAALPDALPARGAALRRLAQALVDTDSLALPDA
RALHPKAAATLDDWAARGWVDVEEIGWADAPVPKAVDNLLAAGGRTVPPALTDQQAEALDAIRAAQGFAPFLLHGVTGSG
KTEVYLHALASLLDARPDAQALVLVPEINLTPQFEAAFRARFAGALADDAIVTLHSGLAEGERARNWLAAHTGRARIVLG
TRLAVLASMPTLALIVVDEEHEPAYKQQEGLRYSARDLAVWRAKQLGITVVLGSATPSLESWWQAEQGRYTRLTLSRRAV
ADATLPTVRLIDLEEERRRGRASMGGLSGPLVAALKARLERGEQSLVFLNRRGYAPQLACDACGWVAGCPRCSAYVVLHK
PEHALRCHHCGWESRIPRSCPECGNVDIAPLGRGTQRIEEALAETVPGARVLRIDADSTRRKGSAQALFSDVHAGEVDIL
VGTQMIAKGHDFQRVSLVGVLNADTALFSHDFRASERLFAQLMQVSGRAGRAGLPGEVLVQTRYPRHALYHALGRQDYVG
FANSTLGERRDAHLPPFVYQALLRAEGRTLDAALAFLLQAAAALPGLPGADRVTVYDAVPMTIVKVANVHRAQLLLESAS
RAALQHALRAWQPELRALKGVLRWSVEVDPLDI

Sequences:

>Translated_753_residues
MSGTYLRVALDHPLATLFDYRCDAQPVPGPGTLVQVPFGKRQAVGLVCEVTTHTDVPPSRLRAVDAICTDLPPLSPDWLA
LVSFAADYYQRGRGEVALPALPQALRDAGRWGRLLAPEVRYRPTEAGRAALPDALPARGAALRRLAQALVDTDSLALPDA
RALHPKAAATLDDWAARGWVDVEEIGWADAPVPKAVDNLLAAGGRTVPPALTDQQAEALDAIRAAQGFAPFLLHGVTGSG
KTEVYLHALASLLDARPDAQALVLVPEINLTPQFEAAFRARFAGALADDAIVTLHSGLAEGERARNWLAAHTGRARIVLG
TRLAVLASMPTLALIVVDEEHEPAYKQQEGLRYSARDLAVWRAKQLGITVVLGSATPSLESWWQAEQGRYTRLTLSRRAV
ADATLPTVRLIDLEEERRRGRASMGGLSGPLVAALKARLERGEQSLVFLNRRGYAPQLACDACGWVAGCPRCSAYVVLHK
PEHALRCHHCGWESRIPRSCPECGNVDIAPLGRGTQRIEEALAETVPGARVLRIDADSTRRKGSAQALFSDVHAGEVDIL
VGTQMIAKGHDFQRVSLVGVLNADTALFSHDFRASERLFAQLMQVSGRAGRAGLPGEVLVQTRYPRHALYHALGRQDYVG
FANSTLGERRDAHLPPFVYQALLRAEGRTLDAALAFLLQAAAALPGLPGADRVTVYDAVPMTIVKVANVHRAQLLLESAS
RAALQHALRAWQPELRALKGVLRWSVEVDPLDI
>Mature_752_residues
SGTYLRVALDHPLATLFDYRCDAQPVPGPGTLVQVPFGKRQAVGLVCEVTTHTDVPPSRLRAVDAICTDLPPLSPDWLAL
VSFAADYYQRGRGEVALPALPQALRDAGRWGRLLAPEVRYRPTEAGRAALPDALPARGAALRRLAQALVDTDSLALPDAR
ALHPKAAATLDDWAARGWVDVEEIGWADAPVPKAVDNLLAAGGRTVPPALTDQQAEALDAIRAAQGFAPFLLHGVTGSGK
TEVYLHALASLLDARPDAQALVLVPEINLTPQFEAAFRARFAGALADDAIVTLHSGLAEGERARNWLAAHTGRARIVLGT
RLAVLASMPTLALIVVDEEHEPAYKQQEGLRYSARDLAVWRAKQLGITVVLGSATPSLESWWQAEQGRYTRLTLSRRAVA
DATLPTVRLIDLEEERRRGRASMGGLSGPLVAALKARLERGEQSLVFLNRRGYAPQLACDACGWVAGCPRCSAYVVLHKP
EHALRCHHCGWESRIPRSCPECGNVDIAPLGRGTQRIEEALAETVPGARVLRIDADSTRRKGSAQALFSDVHAGEVDILV
GTQMIAKGHDFQRVSLVGVLNADTALFSHDFRASERLFAQLMQVSGRAGRAGLPGEVLVQTRYPRHALYHALGRQDYVGF
ANSTLGERRDAHLPPFVYQALLRAEGRTLDAALAFLLQAAAALPGLPGADRVTVYDAVPMTIVKVANVHRAQLLLESASR
AALQHALRAWQPELRALKGVLRWSVEVDPLDI

Specific function: Recognizes a specific hairpin sequence on phiX ssDNA. This structure is then recognized and bound by proteins priB and priC. Formation of the primosome proceeds with the subsequent actions of dnaB, dnaC, dnaT and primase. PriA then functions as a helicase

COG id: COG1198

COG function: function code L; Primosomal protein N' (replication factor Y) - superfamily II helicase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 helicase C-terminal domain [H]

Homologues:

Organism=Escherichia coli, GI1790370, Length=763, Percent_Identity=41.0222804718218, Blast_Score=475, Evalue=1e-135,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR014001
- InterPro:   IPR011545
- InterPro:   IPR001650
- InterPro:   IPR014021
- InterPro:   IPR005259 [H]

Pfam domain/function: PF00270 DEAD; PF00271 Helicase_C [H]

EC number: NA

Molecular weight: Translated: 81385; Mature: 81254

Theoretical pI: Translated: 8.09; Mature: 8.09

Prosite motif: PS00036 BZIP_BASIC

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
0.8 %Met     (Translated Protein)
2.3 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
0.7 %Met     (Mature Protein)
2.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSGTYLRVALDHPLATLFDYRCDAQPVPGPGTLVQVPFGKRQAVGLVCEVTTHTDVPPSR
CCCCEEEEEECCCHHHHHHCCCCCCCCCCCCCEEECCCCCCCCEEEEEEECCCCCCCHHH
LRAVDAICTDLPPLSPDWLALVSFAADYYQRGRGEVALPALPQALRDAGRWGRLLAPEVR
HHHHHHHHHCCCCCCHHHHHHHHHHHHHHHCCCCCEECCHHHHHHHHHCCCCCCCCCHHC
YRPTEAGRAALPDALPARGAALRRLAQALVDTDSLALPDARALHPKAAATLDDWAARGWV
CCCCCCCCCCCCCCCCCCHHHHHHHHHHHHCCCCCCCCCCHHCCCHHHCCHHHHHHCCCC
DVEEIGWADAPVPKAVDNLLAAGGRTVPPALTDQQAEALDAIRAAQGFAPFLLHGVTGSG
CHHHCCCCCCCCHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHCCCCHHHEECCCCCC
KTEVYLHALASLLDARPDAQALVLVPEINLTPQFEAAFRARFAGALADDAIVTLHSGLAE
CHHHHHHHHHHHHHCCCCCCEEEEEECCCCCCCHHHHHHHHHHHHHCCCHHEEEHHCCCC
GERARNWLAAHTGRARIVLGTRLAVLASMPTLALIVVDEEHEPAYKQQEGLRYSARDLAV
CHHHHHHHHHCCCCEEEEECHHHHHHHCCCEEEEEEEECCCCCCHHHHHCCCCCHHHHHH
WRAKQLGITVVLGSATPSLESWWQAEQGRYTRLTLSRRAVADATLPTVRLIDLEEERRRG
HHHHHCCEEEEECCCCCCHHHHHHHCCCCEEEEEEHHHHHHCCCCCEEEEEECHHHHHCC
RASMGGLSGPLVAALKARLERGEQSLVFLNRRGYAPQLACDACGWVAGCPRCSAYVVLHK
CHHCCCCCHHHHHHHHHHHHCCCCEEEEEECCCCCCCHHHHHHHHHCCCCCCCEEEEEEC
PEHALRCHHCGWESRIPRSCPECGNVDIAPLGRGTQRIEEALAETVPGARVLRIDADSTR
CCHHHHHHCCCCCCCCCCCCCCCCCCCEECCCCCHHHHHHHHHHHCCCCEEEEECCCCCC
RKGSAQALFSDVHAGEVDILVGTQMIAKGHDFQRVSLVGVLNADTALFSHDFRASERLFA
CCCHHHHHHHHCCCCCEEEEECCHHHHCCCCCEEEEEEEEECCCHHHHHCCCHHHHHHHH
QLMQVSGRAGRAGLPGEVLVQTRYPRHALYHALGRQDYVGFANSTLGERRDAHLPPFVYQ
HHHHHCCCCCCCCCCCCEEEECCCCHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCHHHHH
ALLRAEGRTLDAALAFLLQAAAALPGLPGADRVTVYDAVPMTIVKVANVHRAQLLLESAS
HHHHHCCCHHHHHHHHHHHHHHHCCCCCCCCEEEEEECCHHHHHHHHHHHHHHHHHHHHH
RAALQHALRAWQPELRALKGVLRWSVEVDPLDI
HHHHHHHHHHCCHHHHHHHHHHHHEEECCCCCC
>Mature Secondary Structure 
SGTYLRVALDHPLATLFDYRCDAQPVPGPGTLVQVPFGKRQAVGLVCEVTTHTDVPPSR
CCCEEEEEECCCHHHHHHCCCCCCCCCCCCCEEECCCCCCCCEEEEEEECCCCCCCHHH
LRAVDAICTDLPPLSPDWLALVSFAADYYQRGRGEVALPALPQALRDAGRWGRLLAPEVR
HHHHHHHHHCCCCCCHHHHHHHHHHHHHHHCCCCCEECCHHHHHHHHHCCCCCCCCCHHC
YRPTEAGRAALPDALPARGAALRRLAQALVDTDSLALPDARALHPKAAATLDDWAARGWV
CCCCCCCCCCCCCCCCCCHHHHHHHHHHHHCCCCCCCCCCHHCCCHHHCCHHHHHHCCCC
DVEEIGWADAPVPKAVDNLLAAGGRTVPPALTDQQAEALDAIRAAQGFAPFLLHGVTGSG
CHHHCCCCCCCCHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHCCCCHHHEECCCCCC
KTEVYLHALASLLDARPDAQALVLVPEINLTPQFEAAFRARFAGALADDAIVTLHSGLAE
CHHHHHHHHHHHHHCCCCCCEEEEEECCCCCCCHHHHHHHHHHHHHCCCHHEEEHHCCCC
GERARNWLAAHTGRARIVLGTRLAVLASMPTLALIVVDEEHEPAYKQQEGLRYSARDLAV
CHHHHHHHHHCCCCEEEEECHHHHHHHCCCEEEEEEEECCCCCCHHHHHCCCCCHHHHHH
WRAKQLGITVVLGSATPSLESWWQAEQGRYTRLTLSRRAVADATLPTVRLIDLEEERRRG
HHHHHCCEEEEECCCCCCHHHHHHHCCCCEEEEEEHHHHHHCCCCCEEEEEECHHHHHCC
RASMGGLSGPLVAALKARLERGEQSLVFLNRRGYAPQLACDACGWVAGCPRCSAYVVLHK
CHHCCCCCHHHHHHHHHHHHCCCCEEEEEECCCCCCCHHHHHHHHHCCCCCCCEEEEEEC
PEHALRCHHCGWESRIPRSCPECGNVDIAPLGRGTQRIEEALAETVPGARVLRIDADSTR
CCHHHHHHCCCCCCCCCCCCCCCCCCCEECCCCCHHHHHHHHHHHCCCCEEEEECCCCCC
RKGSAQALFSDVHAGEVDILVGTQMIAKGHDFQRVSLVGVLNADTALFSHDFRASERLFA
CCCHHHHHHHHCCCCCEEEEECCHHHHCCCCCEEEEEEEEECCCHHHHHCCCHHHHHHHH
QLMQVSGRAGRAGLPGEVLVQTRYPRHALYHALGRQDYVGFANSTLGERRDAHLPPFVYQ
HHHHHCCCCCCCCCCCCEEEECCCCHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCHHHHH
ALLRAEGRTLDAALAFLLQAAAALPGLPGADRVTVYDAVPMTIVKVANVHRAQLLLESAS
HHHHHCCCHHHHHHHHHHHHHHHCCCCCCCCEEEEEECCHHHHHHHHHHHHHHHHHHHHH
RAALQHALRAWQPELRALKGVLRWSVEVDPLDI
HHHHHHHHHHCCHHHHHHHHHHHHEEECCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 7542800 [H]