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The map label for this gene is alkJ [H]

Identifier: 78064740

GI number: 78064740

Start: 95773

End: 97506

Strand: Direct

Name: alkJ [H]

Synonym: Bcep18194_A3263

Alternate gene names: 78064740

Gene position: 95773-97506 (Clockwise)

Preceding gene: 78064737

Following gene: 78064742

Centisome position: 2.59

GC content: 68.11

Gene sequence:

>1734_bases
ATGCTGGAAATGACGCGCGGCGTTGCGCCGCGTGACAGGAGACAAACCGTGACTACCCAACGCACGCTAGAAGGCGAATT
CGACTACGTGATCGTCGGCGCGGGCACCGCGGGCTGCGTGCTCGCGAACCGCCTGACCGAAGATCCCGATATCCATGTGC
TGCTGCTCGAAGCAGGCGGCAAGGACGACTATCACTGGATCCATATCCCGGTCGGCTATCTGTATTGCATCGGCAACCCG
CGCACCGACTGGCTGTACAAGACGCAGCCCGAAGCGGCGCTCAACGGCCGTGCGCTGTCGTATCCGCGCGGCCGCGTGCT
CGGCGGCTGCTCGTCGATCAACGGGATGATCTACATGCGTGGCCAGCGCGAGGATTACGACAGCTGGGCACAGGAAACCG
GCGACGCCGGCTGGTCGTGGGACAGCGTGCTGCCGATCTTCAAGCGCAGCGAGGATCACCATGCGGGCGAGAGCGACGCG
CACGGCGCGGGCGGCTACTGGCGCGTCGAGAAGCAGCGGCTGCGCTGGGAGATCCTCGAATCGTTCGCGCAGGCTGCGCA
GCAGACGGGCATCCCGGCAACCGACGATTTCAACCGCGGCGACAATGCCGGGGTCGGCTATTTCGAGGTGAACCAAAAGC
GCGGCGTGCGCTGGAATACGTCGAAGGCGTTCCTGCGGCCCGCGATGGCGCGCCCGAACCTGACCGTGATCACCGGCGCG
CAGGCGCAACGCGTGATCTTCGACGGGCGGCGCGCGGTCGGCGTCGAATATCACGGCGGCGGCACCGATTACGTTGCACG
GGCGCGCTCGGAGGTGCTGCTGACGTCCGGCGCGGTGAATTCGCCGCAGCTGCTCGAACTGTCGGGCATCGGCGACGGCC
AGCGGCTGCAGGCGCTCGGCATCGACGTCGTGCAGGATCTGCCGGGCGTCGGCGAAAACCTGCAGGATCACCTGCAATTG
CGGATGGCGTTTCGCGTCGAAGGCGTGCGCACGCTCAACACGCTGTCCGCGCACTGGTGGGGCAAGCTGATGATCGGCGC
CGAATATGCGTTGCTGCAGCGCGGGCCGATGTCGATGGCGCCGTCGCAGCTCGGCGCGTTCGCCAAATCCGATCCCGATG
ATCCCGCGCTCACGCGCCCCGATCTCGAATACCACGTGCAGCCGCTGTCGCTCGAACGCTTCGGCGAGCCGCTGCACAGC
TTCAACGCGTTTACGGCGTCCGTGTGCCATTTGCGGCCGACGTCGCGCGGCAGCGTGCATATCGCGAGCGCCGATCCGGG
CGCGGCACCCGCGATCGCGCCGAACTATCTGTCGACCGACCACGATCGTCGCGTCGCCGCGAACGCGCTGCGGCTCACGC
GCCGGATCGCGTCCGCGCCGGCGCTCGCGCGCTATCGTCCCGAGGAAATCCTGCCCGGCCTGCAGTACCGGAGCGAAGCC
GAGCTGATCGAGGCGGCCGGCGCGGTCGGCACGACGATCTTCCATCCGGTCGGCACCTGCCGGATGGGGCGTGCGGACGA
CGAGCGCGCAGTGGTCGATAGCCGGCTGCGCGTGCGCGGCATCGCCGGGCTGCGGATCGTCGATGCATCGGTGATGCCGT
TCATTACGTCGGGCAACACCAATTCGCCGACGCTGATGATCGCCGAGCGCGCGAGCGACATGATTCGTGCCGATCGCCGC
GCGGCGCGCGAAGCGACGCCGGTGCGCACGGAGCCGGCGGTGACGGCCGCGTGA

Upstream 100 bases:

>100_bases
TAACGCAGTTCAGCATAACTCATTGGATTGGCGGCCGGCGGCCGGAGTACGCTTCATCGGTCGTGGCGCGCGGGGCCGCA
AGCGCGGCTCGCGACGCACT

Downstream 100 bases:

>100_bases
CGGAACGCTGACACGCACGACAGCGGGCCGTCATGATGACGCCCGCTGATTCGCTATGCGAAACAAGCTGCCGTTGCAGC
GCCGGTGCCGATGCTGATGG

Product: glucose-methanol-choline oxidoreductase

Products: Betaine Aldehyde; Reduced Acceptor. [C]

Alternate protein names: NA

Number of amino acids: Translated: 577; Mature: 577

Protein sequence:

>577_residues
MLEMTRGVAPRDRRQTVTTQRTLEGEFDYVIVGAGTAGCVLANRLTEDPDIHVLLLEAGGKDDYHWIHIPVGYLYCIGNP
RTDWLYKTQPEAALNGRALSYPRGRVLGGCSSINGMIYMRGQREDYDSWAQETGDAGWSWDSVLPIFKRSEDHHAGESDA
HGAGGYWRVEKQRLRWEILESFAQAAQQTGIPATDDFNRGDNAGVGYFEVNQKRGVRWNTSKAFLRPAMARPNLTVITGA
QAQRVIFDGRRAVGVEYHGGGTDYVARARSEVLLTSGAVNSPQLLELSGIGDGQRLQALGIDVVQDLPGVGENLQDHLQL
RMAFRVEGVRTLNTLSAHWWGKLMIGAEYALLQRGPMSMAPSQLGAFAKSDPDDPALTRPDLEYHVQPLSLERFGEPLHS
FNAFTASVCHLRPTSRGSVHIASADPGAAPAIAPNYLSTDHDRRVAANALRLTRRIASAPALARYRPEEILPGLQYRSEA
ELIEAAGAVGTTIFHPVGTCRMGRADDERAVVDSRLRVRGIAGLRIVDASVMPFITSGNTNSPTLMIAERASDMIRADRR
AAREATPVRTEPAVTAA

Sequences:

>Translated_577_residues
MLEMTRGVAPRDRRQTVTTQRTLEGEFDYVIVGAGTAGCVLANRLTEDPDIHVLLLEAGGKDDYHWIHIPVGYLYCIGNP
RTDWLYKTQPEAALNGRALSYPRGRVLGGCSSINGMIYMRGQREDYDSWAQETGDAGWSWDSVLPIFKRSEDHHAGESDA
HGAGGYWRVEKQRLRWEILESFAQAAQQTGIPATDDFNRGDNAGVGYFEVNQKRGVRWNTSKAFLRPAMARPNLTVITGA
QAQRVIFDGRRAVGVEYHGGGTDYVARARSEVLLTSGAVNSPQLLELSGIGDGQRLQALGIDVVQDLPGVGENLQDHLQL
RMAFRVEGVRTLNTLSAHWWGKLMIGAEYALLQRGPMSMAPSQLGAFAKSDPDDPALTRPDLEYHVQPLSLERFGEPLHS
FNAFTASVCHLRPTSRGSVHIASADPGAAPAIAPNYLSTDHDRRVAANALRLTRRIASAPALARYRPEEILPGLQYRSEA
ELIEAAGAVGTTIFHPVGTCRMGRADDERAVVDSRLRVRGIAGLRIVDASVMPFITSGNTNSPTLMIAERASDMIRADRR
AAREATPVRTEPAVTAA
>Mature_577_residues
MLEMTRGVAPRDRRQTVTTQRTLEGEFDYVIVGAGTAGCVLANRLTEDPDIHVLLLEAGGKDDYHWIHIPVGYLYCIGNP
RTDWLYKTQPEAALNGRALSYPRGRVLGGCSSINGMIYMRGQREDYDSWAQETGDAGWSWDSVLPIFKRSEDHHAGESDA
HGAGGYWRVEKQRLRWEILESFAQAAQQTGIPATDDFNRGDNAGVGYFEVNQKRGVRWNTSKAFLRPAMARPNLTVITGA
QAQRVIFDGRRAVGVEYHGGGTDYVARARSEVLLTSGAVNSPQLLELSGIGDGQRLQALGIDVVQDLPGVGENLQDHLQL
RMAFRVEGVRTLNTLSAHWWGKLMIGAEYALLQRGPMSMAPSQLGAFAKSDPDDPALTRPDLEYHVQPLSLERFGEPLHS
FNAFTASVCHLRPTSRGSVHIASADPGAAPAIAPNYLSTDHDRRVAANALRLTRRIASAPALARYRPEEILPGLQYRSEA
ELIEAAGAVGTTIFHPVGTCRMGRADDERAVVDSRLRVRGIAGLRIVDASVMPFITSGNTNSPTLMIAERASDMIRADRR
AAREATPVRTEPAVTAA

Specific function: Converts aliphatic medium-chain-length alcohols into aldehydes. May be linked to the electron transfer chain [H]

COG id: COG2303

COG function: function code E; Choline dehydrogenase and related flavoproteins

Gene ontology:

Cell location: Cell inner membrane [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the GMC oxidoreductase family [H]

Homologues:

Organism=Homo sapiens, GI217272839, Length=550, Percent_Identity=41.4545454545455, Blast_Score=401, Evalue=1e-112,
Organism=Escherichia coli, GI1786503, Length=552, Percent_Identity=40.7608695652174, Blast_Score=389, Evalue=1e-109,
Organism=Caenorhabditis elegans, GI17532301, Length=558, Percent_Identity=34.7670250896057, Blast_Score=343, Evalue=2e-94,
Organism=Drosophila melanogaster, GI45549471, Length=597, Percent_Identity=36.6834170854271, Blast_Score=345, Evalue=7e-95,
Organism=Drosophila melanogaster, GI45551458, Length=601, Percent_Identity=36.7720465890183, Blast_Score=344, Evalue=9e-95,
Organism=Drosophila melanogaster, GI24642055, Length=602, Percent_Identity=36.046511627907, Blast_Score=342, Evalue=5e-94,
Organism=Drosophila melanogaster, GI24642042, Length=575, Percent_Identity=36, Blast_Score=328, Evalue=7e-90,
Organism=Drosophila melanogaster, GI24642059, Length=569, Percent_Identity=36.2038664323374, Blast_Score=320, Evalue=2e-87,
Organism=Drosophila melanogaster, GI24642048, Length=574, Percent_Identity=35.0174216027875, Blast_Score=311, Evalue=1e-84,
Organism=Drosophila melanogaster, GI24650267, Length=569, Percent_Identity=35.1493848857645, Blast_Score=296, Evalue=3e-80,
Organism=Drosophila melanogaster, GI18859995, Length=592, Percent_Identity=34.6283783783784, Blast_Score=291, Evalue=7e-79,
Organism=Drosophila melanogaster, GI24642035, Length=579, Percent_Identity=32.9879101899827, Blast_Score=285, Evalue=4e-77,
Organism=Drosophila melanogaster, GI24642039, Length=577, Percent_Identity=36.0485268630849, Blast_Score=284, Evalue=1e-76,
Organism=Drosophila melanogaster, GI17137792, Length=557, Percent_Identity=34.8294434470377, Blast_Score=284, Evalue=1e-76,
Organism=Drosophila melanogaster, GI18859993, Length=589, Percent_Identity=31.748726655348, Blast_Score=272, Evalue=4e-73,
Organism=Drosophila melanogaster, GI24642037, Length=578, Percent_Identity=32.6989619377163, Blast_Score=270, Evalue=3e-72,
Organism=Drosophila melanogaster, GI24642051, Length=575, Percent_Identity=32.3478260869565, Blast_Score=259, Evalue=5e-69,
Organism=Drosophila melanogaster, GI24642057, Length=594, Percent_Identity=28.956228956229, Blast_Score=168, Evalue=1e-41,
Organism=Drosophila melanogaster, GI24645930, Length=582, Percent_Identity=28.1786941580756, Blast_Score=150, Evalue=2e-36,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012132
- InterPro:   IPR000172
- InterPro:   IPR007867 [H]

Pfam domain/function: PF05199 GMC_oxred_C; PF00732 GMC_oxred_N [H]

EC number: 1.1.99.1 [C]

Molecular weight: Translated: 63156; Mature: 63156

Theoretical pI: Translated: 7.07; Mature: 7.07

Prosite motif: PS00624 GMC_OXRED_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLEMTRGVAPRDRRQTVTTQRTLEGEFDYVIVGAGTAGCVLANRLTEDPDIHVLLLEAGG
CCCCCCCCCCCCHHHHHHHHHEECCCCCEEEEECCCHHHHHHHHCCCCCCEEEEEEECCC
KDDYHWIHIPVGYLYCIGNPRTDWLYKTQPEAALNGRALSYPRGRVLGGCSSINGMIYMR
CCCEEEEEEEEEEEEECCCCCCCCEEECCCCHHHCCCEECCCCCCEEECCCCCCCEEEEE
GQREDYDSWAQETGDAGWSWDSVLPIFKRSEDHHAGESDAHGAGGYWRVEKQRLRWEILE
CCCCCHHHHHHHCCCCCCCHHHHHHHHHCCCCCCCCCCCCCCCCCEEEEHHHHHHHHHHH
SFAQAAQQTGIPATDDFNRGDNAGVGYFEVNQKRGVRWNTSKAFLRPAMARPNLTVITGA
HHHHHHHHCCCCCCCCCCCCCCCCCEEEEECCCCCCEECCCHHHHHHHHCCCCEEEEECC
QAQRVIFDGRRAVGVEYHGGGTDYVARARSEVLLTSGAVNSPQLLELSGIGDGQRLQALG
CCCEEEECCCEEEEEEEECCCCHHHHHCCCCEEEECCCCCCCCEEEEECCCCCCEEHHHC
IDVVQDLPGVGENLQDHLQLRMAFRVEGVRTLNTLSAHWWGKLMIGAEYALLQRGPMSMA
HHHHHHCCCCCCCHHHHHHHHHHHHHCCCHHHHHHHHHHHEEEEECCHHHHHCCCCCCCC
PSQLGAFAKSDPDDPALTRPDLEYHVQPLSLERFGEPLHSFNAFTASVCHLRPTSRGSVH
HHHHHHHHCCCCCCCCCCCCCHHEEECCCCHHHHCCHHHHHHHHHEEEEEECCCCCCCEE
IASADPGAAPAIAPNYLSTDHDRRVAANALRLTRRIASAPALARYRPEEILPGLQYRSEA
EECCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHCCCHHHHCCHHHHCCCCCCCCHH
ELIEAAGAVGTTIFHPVGTCRMGRADDERAVVDSRLRVRGIAGLRIVDASVMPFITSGNT
HHHHHHCCCCHHHHCCCCCCCCCCCCCHHHHHHHHHEEEECCCEEEECCCCCEEEECCCC
NSPTLMIAERASDMIRADRRAAREATPVRTEPAVTAA
CCCEEEEEHHHHHHHHHHHHHHHCCCCCCCCCCCCCC
>Mature Secondary Structure
MLEMTRGVAPRDRRQTVTTQRTLEGEFDYVIVGAGTAGCVLANRLTEDPDIHVLLLEAGG
CCCCCCCCCCCCHHHHHHHHHEECCCCCEEEEECCCHHHHHHHHCCCCCCEEEEEEECCC
KDDYHWIHIPVGYLYCIGNPRTDWLYKTQPEAALNGRALSYPRGRVLGGCSSINGMIYMR
CCCEEEEEEEEEEEEECCCCCCCCEEECCCCHHHCCCEECCCCCCEEECCCCCCCEEEEE
GQREDYDSWAQETGDAGWSWDSVLPIFKRSEDHHAGESDAHGAGGYWRVEKQRLRWEILE
CCCCCHHHHHHHCCCCCCCHHHHHHHHHCCCCCCCCCCCCCCCCCEEEEHHHHHHHHHHH
SFAQAAQQTGIPATDDFNRGDNAGVGYFEVNQKRGVRWNTSKAFLRPAMARPNLTVITGA
HHHHHHHHCCCCCCCCCCCCCCCCCEEEEECCCCCCEECCCHHHHHHHHCCCCEEEEECC
QAQRVIFDGRRAVGVEYHGGGTDYVARARSEVLLTSGAVNSPQLLELSGIGDGQRLQALG
CCCEEEECCCEEEEEEEECCCCHHHHHCCCCEEEECCCCCCCCEEEEECCCCCCEEHHHC
IDVVQDLPGVGENLQDHLQLRMAFRVEGVRTLNTLSAHWWGKLMIGAEYALLQRGPMSMA
HHHHHHCCCCCCCHHHHHHHHHHHHHCCCHHHHHHHHHHHEEEEECCHHHHHCCCCCCCC
PSQLGAFAKSDPDDPALTRPDLEYHVQPLSLERFGEPLHSFNAFTASVCHLRPTSRGSVH
HHHHHHHHCCCCCCCCCCCCCHHEEECCCCHHHHCCHHHHHHHHHEEEEEECCCCCCCEE
IASADPGAAPAIAPNYLSTDHDRRVAANALRLTRRIASAPALARYRPEEILPGLQYRSEA
EECCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHCCCHHHHCCHHHHCCCCCCCCHH
ELIEAAGAVGTTIFHPVGTCRMGRADDERAVVDSRLRVRGIAGLRIVDASVMPFITSGNT
HHHHHHCCCCHHHHCCCCCCCCCCCCCHHHHHHHHHEEEECCCEEEECCCCCEEEECCCC
NSPTLMIAERASDMIRADRRAAREATPVRTEPAVTAA
CCCEEEEEHHHHHHHHHHHHHHHCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: Coenzyme Q [C]

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): 7 {choline}} 1.1 {phenazine}} [C]

Substrates: Choline; Acceptor [C]

Specific reaction: Choline + Acceptor = Betaine Aldehyde + Reduced Acceptor. [C]

General reaction: Redox reaction [C]

Inhibitor: 2-Dimethylaminoethanol; Betaine aldehyde; Monoethanolamine; Semicarbazide [C]

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 1453953 [H]