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The map label for this gene is outE [H]

Identifier: 78064720

GI number: 78064720

Start: 73275

End: 74774

Strand: Reverse

Name: outE [H]

Synonym: Bcep18194_A3243

Alternate gene names: 78064720

Gene position: 74774-73275 (Counterclockwise)

Preceding gene: 78064721

Following gene: 78064719

Centisome position: 2.02

GC content: 68.47

Gene sequence:

>1500_bases
GTGAGCGACGTGCTCGCGTCTCCCTCCCACGACGGCGCGCCGGGCGAACGGCAGCCGCCGTCGCCGCTCGCCGCGCGATT
GTTGCCGTACGGCTTCGCGAAGAGCGGCCAGGTGCTGATCGCGCATCAGCTCGACGACACGCTCGAGGTGTGGATCAGCG
AACGCACGAGCGACGCCGCGCTCGCGGAGATCGCGCGCAACTTCGGCTCGATCTCCGTGCATCGCGTGCCGGCCGACGAG
CTCGCGCAGGCGATCAACCATGCGTACGCACGCCAGGACGGCAGCGCCGCGCAGGTGGTCGGCGAGGTCGAAGGCGAAGT
CGACCTGTCGCGGCTGATGCAGGACATCCCCGAAGTCGAAGATCTGCTCGAATCGGAAGACGACGCGCCGATCATCCGGA
TGATCAACGCGCTGCTCACGCAAGCGGCACGCGAACAGGCGTCCGACATTCACATCGAGCCGTTCGAGAACGCATCGGTC
GTGCGCTTTCGCGTCGACGGCACGCTGCGCGATGTCGTGCGGCCGAAGAAGGCGCTGCACGGCGCGCTGATCTCGCGGAT
CAAGATCATGGCGCAGCTCGACATCGCGGAGAAACGCCTGCCGCAGGACGGCCGCATCACGCTGCGCGTCGGCGGACGGC
CGGTCGACGTGCGCGTATCGACGCTGCCGACCGGGCACGGCGAGCGTGCGGTGCTGCGTCTGCTGGAAAAGGATGCGGCG
CGCCTGAACCTCGAAGCGCTCGGGATGGGCCGCGACACGCTCGCGCAATTCGACAAGCTGATCGCGCGCCCGCACGGCAT
CGTGCTCGTCACGGGCCCGACGGGTTCGGGCAAGACGACCACGCTGTATGCGTCGATGTCGCGGCTCGAAACCGCGACGA
CCAACATCATGACCGTCGAGGACCCGATCGAGTACGACCTCGGCGGCATCGGCCAGACGCAGGTGAACGAGCGGATCGGG
ATGACCTTTGCCCGTGCGCTGCGCTCGATCCTGCGCCAGGATCCGGACGTGATCATGATCGGCGAAATCCGCGACCTCGA
AACCGCGCAGATCGCGGTGCAGGCCTCGCTGACGGGCCACCTCGTGCTCGCGACGCTGCACACGAACGATGCGGCCTCGG
CCGTCACGCGTCTGACCGACATGGGCGTCGAGCCCTATCTGCTCGCGTCGTCGCTGCTCGGCGTGCTCGCGCAGCGGCTC
GTGCGGCAGCTCTGCCCCGTCTGCAAGGAAGAACGCCAGGAAGAAGGCCGCACCGTGTGGCATCCGGTCGGCTGCGACAA
GTGCGGGCATTCGGGTTATTCGGGCCGGCGCGGTGTGTACGAACTGCTGCTGGTCGACGAGTCGATCCGCTCGCTGATCC
ACCGCAACGCGGCCGACGCGGAGATTCTCGCCGCCGGCCGTGCGGAAGGGATGCGCACGCTGCGCGACGATGCCGAGCGC
TGGCTCGCGTCGGGCGCGACGTCGCTCGAGGAAGTGCTGCGCGTGACGGGAGGCGCATAG

Upstream 100 bases:

>100_bases
CGGCCCAGCCGTTGCCGGAGGCAACGCCCGCGCAGCCGCAGAGCGTGCCGCAGCAGGCGGTGCCGCAACAGCCGCTGACG
GCCACGCCGGGAGCGTCGCA

Downstream 100 bases:

>100_bases
CGCGATGCCGGCATTCCGTTTCGAAGCAATCGATTCGGCGGGACGCGCGCAGAAAGGCGTCATCGACGCCGACAGCGCAC
GTGCCGCGCGCGGCCAGCTG

Product: type II secretion system protein E

Products: NA

Alternate protein names: Pectic enzymes secretion protein outE; Type II traffic warden ATPase [H]

Number of amino acids: Translated: 499; Mature: 498

Protein sequence:

>499_residues
MSDVLASPSHDGAPGERQPPSPLAARLLPYGFAKSGQVLIAHQLDDTLEVWISERTSDAALAEIARNFGSISVHRVPADE
LAQAINHAYARQDGSAAQVVGEVEGEVDLSRLMQDIPEVEDLLESEDDAPIIRMINALLTQAAREQASDIHIEPFENASV
VRFRVDGTLRDVVRPKKALHGALISRIKIMAQLDIAEKRLPQDGRITLRVGGRPVDVRVSTLPTGHGERAVLRLLEKDAA
RLNLEALGMGRDTLAQFDKLIARPHGIVLVTGPTGSGKTTTLYASMSRLETATTNIMTVEDPIEYDLGGIGQTQVNERIG
MTFARALRSILRQDPDVIMIGEIRDLETAQIAVQASLTGHLVLATLHTNDAASAVTRLTDMGVEPYLLASSLLGVLAQRL
VRQLCPVCKEERQEEGRTVWHPVGCDKCGHSGYSGRRGVYELLLVDESIRSLIHRNAADAEILAAGRAEGMRTLRDDAER
WLASGATSLEEVLRVTGGA

Sequences:

>Translated_499_residues
MSDVLASPSHDGAPGERQPPSPLAARLLPYGFAKSGQVLIAHQLDDTLEVWISERTSDAALAEIARNFGSISVHRVPADE
LAQAINHAYARQDGSAAQVVGEVEGEVDLSRLMQDIPEVEDLLESEDDAPIIRMINALLTQAAREQASDIHIEPFENASV
VRFRVDGTLRDVVRPKKALHGALISRIKIMAQLDIAEKRLPQDGRITLRVGGRPVDVRVSTLPTGHGERAVLRLLEKDAA
RLNLEALGMGRDTLAQFDKLIARPHGIVLVTGPTGSGKTTTLYASMSRLETATTNIMTVEDPIEYDLGGIGQTQVNERIG
MTFARALRSILRQDPDVIMIGEIRDLETAQIAVQASLTGHLVLATLHTNDAASAVTRLTDMGVEPYLLASSLLGVLAQRL
VRQLCPVCKEERQEEGRTVWHPVGCDKCGHSGYSGRRGVYELLLVDESIRSLIHRNAADAEILAAGRAEGMRTLRDDAER
WLASGATSLEEVLRVTGGA
>Mature_498_residues
SDVLASPSHDGAPGERQPPSPLAARLLPYGFAKSGQVLIAHQLDDTLEVWISERTSDAALAEIARNFGSISVHRVPADEL
AQAINHAYARQDGSAAQVVGEVEGEVDLSRLMQDIPEVEDLLESEDDAPIIRMINALLTQAAREQASDIHIEPFENASVV
RFRVDGTLRDVVRPKKALHGALISRIKIMAQLDIAEKRLPQDGRITLRVGGRPVDVRVSTLPTGHGERAVLRLLEKDAAR
LNLEALGMGRDTLAQFDKLIARPHGIVLVTGPTGSGKTTTLYASMSRLETATTNIMTVEDPIEYDLGGIGQTQVNERIGM
TFARALRSILRQDPDVIMIGEIRDLETAQIAVQASLTGHLVLATLHTNDAASAVTRLTDMGVEPYLLASSLLGVLAQRLV
RQLCPVCKEERQEEGRTVWHPVGCDKCGHSGYSGRRGVYELLLVDESIRSLIHRNAADAEILAAGRAEGMRTLRDDAERW
LASGATSLEEVLRVTGGA

Specific function: Involved in a general secretion pathway (GSP) for the export of proteins. Required for the translocation of pectate lyases and cellulases across the outer membrane [H]

COG id: COG2804

COG function: function code NU; Type II secretory pathway, ATPase PulE/Tfp pilus assembly pathway, ATPase PilB

Gene ontology:

Cell location: Cytoplasm (Probable) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the GSP E family [H]

Homologues:

Organism=Escherichia coli, GI1789723, Length=420, Percent_Identity=54.2857142857143, Blast_Score=452, Evalue=1e-128,
Organism=Escherichia coli, GI1786296, Length=442, Percent_Identity=42.3076923076923, Blast_Score=331, Evalue=8e-92,
Organism=Escherichia coli, GI87082188, Length=282, Percent_Identity=35.8156028368794, Blast_Score=120, Evalue=2e-28,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013369
- InterPro:   IPR001482 [H]

Pfam domain/function: PF00437 GSPII_E [H]

EC number: NA

Molecular weight: Translated: 54131; Mature: 54000

Theoretical pI: Translated: 5.27; Mature: 5.27

Prosite motif: PS00662 T2SP_E

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSDVLASPSHDGAPGERQPPSPLAARLLPYGFAKSGQVLIAHQLDDTLEVWISERTSDAA
CCCCCCCCCCCCCCCCCCCCCHHHHHHHCCCCCCCCCEEEEECCCHHHHHHHHCCCCHHH
LAEIARNFGSISVHRVPADELAQAINHAYARQDGSAAQVVGEVEGEVDLSRLMQDIPEVE
HHHHHHHCCCEEEEECCHHHHHHHHHHHHHCCCCCHHHHHHHCCCCHHHHHHHHHHHHHH
DLLESEDDAPIIRMINALLTQAAREQASDIHIEPFENASVVRFRVDGTLRDVVRPKKALH
HHHCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCEEEEEECCHHHHHHHHHHHHH
GALISRIKIMAQLDIAEKRLPQDGRITLRVGGRPVDVRVSTLPTGHGERAVLRLLEKDAA
HHHHHHHHHHHHHHHHHHHCCCCCEEEEEECCEEEEEEEEECCCCCCHHHHHHHHHHHHH
RLNLEALGMGRDTLAQFDKLIARPHGIVLVTGPTGSGKTTTLYASMSRLETATTNIMTVE
HCCHHHCCCCHHHHHHHHHHHCCCCCEEEEECCCCCCCEEEEEHHHHHHHHHHCCEEEEC
DPIEYDLGGIGQTQVNERIGMTFARALRSILRQDPDVIMIGEIRDLETAQIAVQASLTGH
CCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCEEEECCCCCCCHHHEEEEEECCCE
LVLATLHTNDAASAVTRLTDMGVEPYLLASSLLGVLAQRLVRQLCPVCKEERQEEGRTVW
EEEEEEECCHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEE
HPVGCDKCGHSGYSGRRGVYELLLVDESIRSLIHRNAADAEILAAGRAEGMRTLRDDAER
CCCCCCCCCCCCCCCCCCHHHHEEHHHHHHHHHHCCCCCHHHHHCCHHHHHHHHHHHHHH
WLASGATSLEEVLRVTGGA
HHHCCCHHHHHHHHHCCCC
>Mature Secondary Structure 
SDVLASPSHDGAPGERQPPSPLAARLLPYGFAKSGQVLIAHQLDDTLEVWISERTSDAA
CCCCCCCCCCCCCCCCCCCCHHHHHHHCCCCCCCCCEEEEECCCHHHHHHHHCCCCHHH
LAEIARNFGSISVHRVPADELAQAINHAYARQDGSAAQVVGEVEGEVDLSRLMQDIPEVE
HHHHHHHCCCEEEEECCHHHHHHHHHHHHHCCCCCHHHHHHHCCCCHHHHHHHHHHHHHH
DLLESEDDAPIIRMINALLTQAAREQASDIHIEPFENASVVRFRVDGTLRDVVRPKKALH
HHHCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCEEEEEECCHHHHHHHHHHHHH
GALISRIKIMAQLDIAEKRLPQDGRITLRVGGRPVDVRVSTLPTGHGERAVLRLLEKDAA
HHHHHHHHHHHHHHHHHHHCCCCCEEEEEECCEEEEEEEEECCCCCCHHHHHHHHHHHHH
RLNLEALGMGRDTLAQFDKLIARPHGIVLVTGPTGSGKTTTLYASMSRLETATTNIMTVE
HCCHHHCCCCHHHHHHHHHHHCCCCCEEEEECCCCCCCEEEEEHHHHHHHHHHCCEEEEC
DPIEYDLGGIGQTQVNERIGMTFARALRSILRQDPDVIMIGEIRDLETAQIAVQASLTGH
CCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCEEEECCCCCCCHHHEEEEEECCCE
LVLATLHTNDAASAVTRLTDMGVEPYLLASSLLGVLAQRLVRQLCPVCKEERQEEGRTVW
EEEEEEECCHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEE
HPVGCDKCGHSGYSGRRGVYELLLVDESIRSLIHRNAADAEILAAGRAEGMRTLRDDAER
CCCCCCCCCCCCCCCCCCHHHHEEHHHHHHHHHHCCCCCHHHHHCCHHHHHHHHHHHHHH
WLASGATSLEEVLRVTGGA
HHHCCCHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 1429461 [H]