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The map label for this gene is emrB [H]

Identifier: 78064706

GI number: 78064706

Start: 60904

End: 62466

Strand: Reverse

Name: emrB [H]

Synonym: Bcep18194_A3229

Alternate gene names: 78064706

Gene position: 62466-60904 (Counterclockwise)

Preceding gene: 78064707

Following gene: 78064704

Centisome position: 1.69

GC content: 66.73

Gene sequence:

>1563_bases
ATGTCCGCCACCACGGCATCCGCCGCCTCCCCCGCCGCCGAACCCGCGCCGCTGTCCGGCGGTGCGCTCGCGCTGCTCAC
CGTCGGGCTCGCGCTCGGCACCTTCATGGAAGTGCTCGACACGTCGATCGCGAACGTCGCGGTGCCGACCATCTCGGGCA
GCCTCGGCGTCGCGACGAGCGAAGGCACCTGGGTGATCTCGTCGTACTCGGTGGCTTCCGCGATCGCGGTGCCGCTTACC
GGCTGGCTCGCGCGGCGGGTCGGCGAGGTGCGGCTGTTCACCCTGTCGGTGCTTGCGTTCACGGTCGCATCGGCGCTGTG
CGGCCTCGCGACCAACTTCGAGACGCTGATCGCGTTCCGGCTGCTGCAGGGCCTCGTGTCGGGGCCGATGGTGCCGCTGT
CGCAGACGATCCTGATGCGCAGCTACCCGCCCGCGAAGCGCGGGCTCGCGCTCGGGCTATGGGCGATGACGGTGATCGTC
GCACCGATCTTCGGCCCGTTGCTGGGCGGCTGGATCAGCGACAACTACACGTGGCCATGGATCTTCTATATCAACCTGCC
GATCGGTATCTTCTCGGCGACCTGCGCGTATTTCCTGCTGCGCGGCCGCGAGACGAAGACGTCGAAGCAGCGGATCGACG
CGATCGGCCTCGCGCTGCTCGTGATCGGCGTGTCGTGCCTGCAGATGATGCTCGACCTCGGCAAGGACCGTGACTGGTTC
AACTCGACATTCATCGTCGCGCTCGCGCTGATCGCGGTCGTGTCGCTCGCGTTCATGCTCGTGTGGGAGGCGACCGAGAA
GGAGCCGGTGGTCGACCTGTCGCTCTTCAAGGACCGCAACTTCGCGCTCGGCGCGATGATCATCTCGTTCGGCTTCATGG
CGTTCTTCGGCTCGGTCGTGATCTTCCCGCTGTGGCTGCAGACGGTGATGGGCTACACGGCCGGCAAGGCCGGCCTCGCG
ACCGCGCCGGTCGGGCTGCTCGCGCTCGTGCTGTCCCCGCTGATCGGCCGCAACATGCATCGCCTCGACCTGCGGATGGT
CGCGAGCTTCGCGTTCATCGTGTTCGCCGGCGTGTCGATCTGGAACTCGACGTTTACGCTCGACGTGCCGTTCAACCATG
TGATCCTGCCGCGTCTCGTGCAGGGGATCGGCGTCGCGTGCTTCTTCGTGCCGATGACGACGATCACGCTGTCGAGCATC
TCCGACGACCGGCTCGCGAGCGCGTCGGGGCTGTCGAACTTCCTGCGCACGCTGTCGGGCGCGATCGGCACCGCGGCGAG
TTCGACGTTCTGGGAGAACGACGCGATCTATCACCACGCGCGGCTGTCGGAATCGGTGAGCGTGTATGCGCAGAACACCA
CCGACTACCAGGGCGCGCTGTCGCAGCTCGGCATCGTCGGGCAGACGGCCAATGCGCAGCTCAACCAGCTCGTCACGCAG
CAGGGCTTCATGATGGCGACCAACGACTTCTTCCTGCTGTCGGCGTTCATGTTCGGCGCACTCGCGGGGCTCGTGTGGAT
CACGAAGCCGAGAAAGGGTGCGGGGCCGGCGATGGGGCATTGA

Upstream 100 bases:

>100_bases
CCCTTTTTCCGTTTATCCGTTCCACCCGGCCGGGCTGCGCTGCGGCGCACACCCGTGCGTTGTGCCGGCGCGTCGCGCGC
GCCGCGTCTAGGAGATTCCG

Downstream 100 bases:

>100_bases
AGGCGCGGCGGGCGGCCATTGCGCCCGCCTCGTTCGATTTACCGCCCGCTTCGGCGGGCGTCACTCCCTCGCCCGCAACC
CGCCGAACGCCAGATCGTCA

Product: EmrB/QacA family drug resistance transporter

Products: Proton [Cytoplasm]; multidrug [Periplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 520; Mature: 519

Protein sequence:

>520_residues
MSATTASAASPAAEPAPLSGGALALLTVGLALGTFMEVLDTSIANVAVPTISGSLGVATSEGTWVISSYSVASAIAVPLT
GWLARRVGEVRLFTLSVLAFTVASALCGLATNFETLIAFRLLQGLVSGPMVPLSQTILMRSYPPAKRGLALGLWAMTVIV
APIFGPLLGGWISDNYTWPWIFYINLPIGIFSATCAYFLLRGRETKTSKQRIDAIGLALLVIGVSCLQMMLDLGKDRDWF
NSTFIVALALIAVVSLAFMLVWEATEKEPVVDLSLFKDRNFALGAMIISFGFMAFFGSVVIFPLWLQTVMGYTAGKAGLA
TAPVGLLALVLSPLIGRNMHRLDLRMVASFAFIVFAGVSIWNSTFTLDVPFNHVILPRLVQGIGVACFFVPMTTITLSSI
SDDRLASASGLSNFLRTLSGAIGTAASSTFWENDAIYHHARLSESVSVYAQNTTDYQGALSQLGIVGQTANAQLNQLVTQ
QGFMMATNDFFLLSAFMFGALAGLVWITKPRKGAGPAMGH

Sequences:

>Translated_520_residues
MSATTASAASPAAEPAPLSGGALALLTVGLALGTFMEVLDTSIANVAVPTISGSLGVATSEGTWVISSYSVASAIAVPLT
GWLARRVGEVRLFTLSVLAFTVASALCGLATNFETLIAFRLLQGLVSGPMVPLSQTILMRSYPPAKRGLALGLWAMTVIV
APIFGPLLGGWISDNYTWPWIFYINLPIGIFSATCAYFLLRGRETKTSKQRIDAIGLALLVIGVSCLQMMLDLGKDRDWF
NSTFIVALALIAVVSLAFMLVWEATEKEPVVDLSLFKDRNFALGAMIISFGFMAFFGSVVIFPLWLQTVMGYTAGKAGLA
TAPVGLLALVLSPLIGRNMHRLDLRMVASFAFIVFAGVSIWNSTFTLDVPFNHVILPRLVQGIGVACFFVPMTTITLSSI
SDDRLASASGLSNFLRTLSGAIGTAASSTFWENDAIYHHARLSESVSVYAQNTTDYQGALSQLGIVGQTANAQLNQLVTQ
QGFMMATNDFFLLSAFMFGALAGLVWITKPRKGAGPAMGH
>Mature_519_residues
SATTASAASPAAEPAPLSGGALALLTVGLALGTFMEVLDTSIANVAVPTISGSLGVATSEGTWVISSYSVASAIAVPLTG
WLARRVGEVRLFTLSVLAFTVASALCGLATNFETLIAFRLLQGLVSGPMVPLSQTILMRSYPPAKRGLALGLWAMTVIVA
PIFGPLLGGWISDNYTWPWIFYINLPIGIFSATCAYFLLRGRETKTSKQRIDAIGLALLVIGVSCLQMMLDLGKDRDWFN
STFIVALALIAVVSLAFMLVWEATEKEPVVDLSLFKDRNFALGAMIISFGFMAFFGSVVIFPLWLQTVMGYTAGKAGLAT
APVGLLALVLSPLIGRNMHRLDLRMVASFAFIVFAGVSIWNSTFTLDVPFNHVILPRLVQGIGVACFFVPMTTITLSSIS
DDRLASASGLSNFLRTLSGAIGTAASSTFWENDAIYHHARLSESVSVYAQNTTDYQGALSQLGIVGQTANAQLNQLVTQQ
GFMMATNDFFLLSAFMFGALAGLVWITKPRKGAGPAMGH

Specific function: Translocase that confers resistance to substances of high hydrophobicity [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the major facilitator superfamily. EmrB family [H]

Homologues:

Organism=Escherichia coli, GI1789042, Length=498, Percent_Identity=52.6104417670683, Blast_Score=528, Evalue=1e-151,
Organism=Escherichia coli, GI1788710, Length=514, Percent_Identity=47.6653696498054, Blast_Score=491, Evalue=1e-140,
Organism=Escherichia coli, GI1790195, Length=407, Percent_Identity=31.2039312039312, Blast_Score=172, Evalue=4e-44,
Organism=Escherichia coli, GI1788392, Length=402, Percent_Identity=29.8507462686567, Blast_Score=127, Evalue=2e-30,
Organism=Escherichia coli, GI87081983, Length=419, Percent_Identity=25.5369928400955, Blast_Score=123, Evalue=2e-29,
Organism=Escherichia coli, GI87082320, Length=176, Percent_Identity=26.7045454545455, Blast_Score=62, Evalue=9e-11,
Organism=Saccharomyces cerevisiae, GI6322958, Length=215, Percent_Identity=29.7674418604651, Blast_Score=78, Evalue=4e-15,
Organism=Saccharomyces cerevisiae, GI6325455, Length=129, Percent_Identity=29.4573643410853, Blast_Score=72, Evalue=2e-13,
Organism=Saccharomyces cerevisiae, GI6321663, Length=150, Percent_Identity=27.3333333333333, Blast_Score=68, Evalue=3e-12,
Organism=Saccharomyces cerevisiae, GI6319770, Length=307, Percent_Identity=24.1042345276873, Blast_Score=63, Evalue=9e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004638
- InterPro:   IPR020846
- InterPro:   IPR011701
- InterPro:   IPR016196 [H]

Pfam domain/function: PF07690 MFS_1 [H]

EC number: NA

Molecular weight: Translated: 55330; Mature: 55199

Theoretical pI: Translated: 8.31; Mature: 8.31

Prosite motif: PS50850 MFS

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
3.5 %Met     (Translated Protein)
4.2 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
3.3 %Met     (Mature Protein)
4.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSATTASAASPAAEPAPLSGGALALLTVGLALGTFMEVLDTSIANVAVPTISGSLGVATS
CCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHEEEECCCCCCCEEEC
EGTWVISSYSVASAIAVPLTGWLARRVGEVRLFTLSVLAFTVASALCGLATNFETLIAFR
CCCEEEECHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHH
LLQGLVSGPMVPLSQTILMRSYPPAKRGLALGLWAMTVIVAPIFGPLLGGWISDNYTWPW
HHHHHHCCCCCCHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCHHCCCCCCEE
IFYINLPIGIFSATCAYFLLRGRETKTSKQRIDAIGLALLVIGVSCLQMMLDLGKDRDWF
EEEEECCHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHH
NSTFIVALALIAVVSLAFMLVWEATEKEPVVDLSLFKDRNFALGAMIISFGFMAFFGSVV
HHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEHEECCCCCHHHHHHHHHHHHHHHHHHHH
IFPLWLQTVMGYTAGKAGLATAPVGLLALVLSPLIGRNMHRLDLRMVASFAFIVFAGVSI
HHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHH
WNSTFTLDVPFNHVILPRLVQGIGVACFFVPMTTITLSSISDDRLASASGLSNFLRTLSG
HCCEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHH
AIGTAASSTFWENDAIYHHARLSESVSVYAQNTTDYQGALSQLGIVGQTANAQLNQLVTQ
HHCCCCCCCCCCCCCHHHHHHHCCCEEEEECCCCCHHHHHHHHCCCCCCCCHHHHHHHHH
QGFMMATNDFFLLSAFMFGALAGLVWITKPRKGAGPAMGH
CCCEEECCHHHHHHHHHHHHHHHHHEEECCCCCCCCCCCC
>Mature Secondary Structure 
SATTASAASPAAEPAPLSGGALALLTVGLALGTFMEVLDTSIANVAVPTISGSLGVATS
CCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHEEEECCCCCCCEEEC
EGTWVISSYSVASAIAVPLTGWLARRVGEVRLFTLSVLAFTVASALCGLATNFETLIAFR
CCCEEEECHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHH
LLQGLVSGPMVPLSQTILMRSYPPAKRGLALGLWAMTVIVAPIFGPLLGGWISDNYTWPW
HHHHHHCCCCCCHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCHHCCCCCCEE
IFYINLPIGIFSATCAYFLLRGRETKTSKQRIDAIGLALLVIGVSCLQMMLDLGKDRDWF
EEEEECCHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHH
NSTFIVALALIAVVSLAFMLVWEATEKEPVVDLSLFKDRNFALGAMIISFGFMAFFGSVV
HHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEHEECCCCCHHHHHHHHHHHHHHHHHHHH
IFPLWLQTVMGYTAGKAGLATAPVGLLALVLSPLIGRNMHRLDLRMVASFAFIVFAGVSI
HHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHH
WNSTFTLDVPFNHVILPRLVQGIGVACFFVPMTTITLSSISDDRLASASGLSNFLRTLSG
HCCEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHH
AIGTAASSTFWENDAIYHHARLSESVSVYAQNTTDYQGALSQLGIVGQTANAQLNQLVTQ
HHCCCCCCCCCCCCCHHHHHHHCCCEEEEECCCCCHHHHHHHHCCCCCCCCHHHHHHHHH
QGFMMATNDFFLLSAFMFGALAGLVWITKPRKGAGPAMGH
CCCEEECCHHHHHHHHHHHHHHHHHEEECCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Proton [Periplasm]; multidrug [Cytoplasm] [C]

Specific reaction: Proton [Periplasm] + multidrug [Cytoplasm] = Proton [Cytoplasm] + multidrug [Periplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]