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The map label for this gene is speE

Identifier: 161702968

GI number: 161702968

Start: 609129

End: 609986

Strand: Reverse

Name: speE

Synonym: Bcep18194_A3721

Alternate gene names: 161702968

Gene position: 609986-609129 (Counterclockwise)

Preceding gene: 78065200

Following gene: 78065196

Centisome position: 16.51

GC content: 69.93

Gene sequence:

>858_bases
GTGAGCACAACTCTTCTCTTTCACCCAACGCCCGACGCCGCGTACGGCTTCCCGAACGCGCGGCGGCTCGCGCGCGTCGC
GTCCCCGCACCAGCAGATCGAAGTCTGGGAGACCCCGCAGCTCGGCCGCCTGTTTACGCTCGACGGCCGGCCGATGACGT
CGGTTGGCGACGAGTACGTCTATCACGAGTGCATGACGCACCCGGCCGCGCTCGCGCATCCGGCGCCGCGCCGGGCGCTC
GTGCTCGGCGGCGGCGACGGCGGCGCGGCGCGCCAGCTGCTCAAGCATGCATGCATCGAGCGGATCGTGATCGCGGAACT
CGACGACGAGGTGGTCGGGATGGCACGCCGTTATCTCGACGACGTGCACCAGGGTGCGCTCGACGACCCGCGTGTCGAGG
TCGTGATCGGCGACGCCGCACATTTCGTCGAGTCGACCGTCGAGCACTTCGATCTCGTCGTGTTCGACCTCACGCCGCCC
GATTCGCCGGCGGCCGGCCTCTATACACGCGCGTTCTACGCGCGGCTCAAGCGGATCCTGACGCCGTGCGGCGCAATCTC
GATGCACCTCGGCTCGCCGGTGTTCCACGCGGCGCGCATCGCCGCGCTGCTCGACGACCTGCGCGCGTGCTTCGCGGTCG
TCGATCCGTTGTCCGCGCACGTGCCGCTCTACGGCTCGCAGTGGCTGATGGCGATCGCGAGCGACACGCTCGACGCCGCC
GCACTGTTCGCACACGACATCGACGAACGGCTCGCGGCCCGCCGCGTCCAGGGCTTGCGCTACTACGACGCGCGGCTGCA
TGCGTCCCTCTTTGCCCTGCCGTGCGCGCTGCGCGATACACTGGGCGTTCGCCGCTGA

Upstream 100 bases:

>100_bases
TTTCCCTTGTCCATCCACAGTGTTCCGGCACGGTTTTCCGTCAAACCGCCCGCCTCCTATAATTCAGACGTCGGAACTGC
CAGCCCTGAGCGGCCCGCCC

Downstream 100 bases:

>100_bases
ACGTCCGCGCGGCCGCCTCGCTTCCGTTTTTCCATCGCGTCCCGGCAGGATTCGCGATGCATGGCACCGGGGCGAAACGC
CCCGGACGCCGCGCCGCCCC

Product: spermidine synthase

Products: NA

Alternate protein names: Putrescine aminopropyltransferase; PAPT; SPDSY [H]

Number of amino acids: Translated: 285; Mature: 284

Protein sequence:

>285_residues
MSTTLLFHPTPDAAYGFPNARRLARVASPHQQIEVWETPQLGRLFTLDGRPMTSVGDEYVYHECMTHPAALAHPAPRRAL
VLGGGDGGAARQLLKHACIERIVIAELDDEVVGMARRYLDDVHQGALDDPRVEVVIGDAAHFVESTVEHFDLVVFDLTPP
DSPAAGLYTRAFYARLKRILTPCGAISMHLGSPVFHAARIAALLDDLRACFAVVDPLSAHVPLYGSQWLMAIASDTLDAA
ALFAHDIDERLAARRVQGLRYYDARLHASLFALPCALRDTLGVRR

Sequences:

>Translated_285_residues
MSTTLLFHPTPDAAYGFPNARRLARVASPHQQIEVWETPQLGRLFTLDGRPMTSVGDEYVYHECMTHPAALAHPAPRRAL
VLGGGDGGAARQLLKHACIERIVIAELDDEVVGMARRYLDDVHQGALDDPRVEVVIGDAAHFVESTVEHFDLVVFDLTPP
DSPAAGLYTRAFYARLKRILTPCGAISMHLGSPVFHAARIAALLDDLRACFAVVDPLSAHVPLYGSQWLMAIASDTLDAA
ALFAHDIDERLAARRVQGLRYYDARLHASLFALPCALRDTLGVRR
>Mature_284_residues
STTLLFHPTPDAAYGFPNARRLARVASPHQQIEVWETPQLGRLFTLDGRPMTSVGDEYVYHECMTHPAALAHPAPRRALV
LGGGDGGAARQLLKHACIERIVIAELDDEVVGMARRYLDDVHQGALDDPRVEVVIGDAAHFVESTVEHFDLVVFDLTPPD
SPAAGLYTRAFYARLKRILTPCGAISMHLGSPVFHAARIAALLDDLRACFAVVDPLSAHVPLYGSQWLMAIASDTLDAAA
LFAHDIDERLAARRVQGLRYYDARLHASLFALPCALRDTLGVRR

Specific function: Catalyzes the production of spermidine from putrescine and decarboxylated S-adenosylmethionine (dcSAM), which serves as an aminopropyl donor [H]

COG id: COG0421

COG function: function code E; Spermidine synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the spermidine/spermine synthase family [H]

Homologues:

Organism=Homo sapiens, GI63253298, Length=256, Percent_Identity=25.78125, Blast_Score=114, Evalue=1e-25,
Organism=Escherichia coli, GI1786312, Length=284, Percent_Identity=33.8028169014084, Blast_Score=157, Evalue=1e-39,
Organism=Caenorhabditis elegans, GI25154106, Length=278, Percent_Identity=29.136690647482, Blast_Score=115, Evalue=3e-26,
Organism=Caenorhabditis elegans, GI212645616, Length=216, Percent_Identity=29.6296296296296, Blast_Score=106, Evalue=1e-23,
Organism=Saccharomyces cerevisiae, GI6323175, Length=274, Percent_Identity=30.6569343065693, Blast_Score=140, Evalue=2e-34,
Organism=Saccharomyces cerevisiae, GI6325326, Length=267, Percent_Identity=27.3408239700375, Blast_Score=125, Evalue=8e-30,
Organism=Drosophila melanogaster, GI24645443, Length=266, Percent_Identity=28.1954887218045, Blast_Score=124, Evalue=6e-29,
Organism=Drosophila melanogaster, GI21355941, Length=205, Percent_Identity=29.2682926829268, Blast_Score=101, Evalue=7e-22,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001045 [H]

Pfam domain/function: PF01564 Spermine_synth [H]

EC number: =2.5.1.16 [H]

Molecular weight: Translated: 31246; Mature: 31115

Theoretical pI: Translated: 6.50; Mature: 6.50

Prosite motif: PS51006 SPERMIDINE_SYNTHASE_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.8 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
3.9 %Cys+Met (Translated Protein)
1.8 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSTTLLFHPTPDAAYGFPNARRLARVASPHQQIEVWETPQLGRLFTLDGRPMTSVGDEYV
CCCEEEEECCCCCCCCCCCHHHHHHHCCCHHHEEEECCCCCCCEEEECCCCCCCCCHHHH
YHECMTHPAALAHPAPRRALVLGGGDGGAARQLLKHACIERIVIAELDDEVVGMARRYLD
HHHHHCCCHHHCCCCCCCEEEEECCCCHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHH
DVHQGALDDPRVEVVIGDAAHFVESTVEHFDLVVFDLTPPDSPAAGLYTRAFYARLKRIL
HHHHCCCCCCCEEEEECCHHHHHHHHHHHHEEEEEECCCCCCCCHHHHHHHHHHHHHHHH
TPCGAISMHLGSPVFHAARIAALLDDLRACFAVVDPLSAHVPLYGSQWLMAIASDTLDAA
CCCCHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHCCHHCCCCCCCCHHHHHHHHHHHHHH
ALFAHDIDERLAARRVQGLRYYDARLHASLFALPCALRDTLGVRR
HHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHCCCC
>Mature Secondary Structure 
STTLLFHPTPDAAYGFPNARRLARVASPHQQIEVWETPQLGRLFTLDGRPMTSVGDEYV
CCEEEEECCCCCCCCCCCHHHHHHHCCCHHHEEEECCCCCCCEEEECCCCCCCCCHHHH
YHECMTHPAALAHPAPRRALVLGGGDGGAARQLLKHACIERIVIAELDDEVVGMARRYLD
HHHHHCCCHHHCCCCCCCEEEEECCCCHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHH
DVHQGALDDPRVEVVIGDAAHFVESTVEHFDLVVFDLTPPDSPAAGLYTRAFYARLKRIL
HHHHCCCCCCCEEEEECCHHHHHHHHHHHHEEEEEECCCCCCCCHHHHHHHHHHHHHHHH
TPCGAISMHLGSPVFHAARIAALLDDLRACFAVVDPLSAHVPLYGSQWLMAIASDTLDAA
CCCCHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHCCHHCCCCCCCCHHHHHHHHHHHHHH
ALFAHDIDERLAARRVQGLRYYDARLHASLFALPCALRDTLGVRR
HHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12700255 [H]