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The map label for this gene is rpoH [H]

Identifier: 78049505

GI number: 78049505

Start: 4532987

End: 4533871

Strand: Reverse

Name: rpoH [H]

Synonym: XCV3949

Alternate gene names: 78049505

Gene position: 4533871-4532987 (Counterclockwise)

Preceding gene: 78049506

Following gene: 78049502

Centisome position: 87.55

GC content: 62.94

Gene sequence:

>885_bases
ATGGTGCCTATGAACCAGACTACCTCGACTGCCCTTGTGGCAAACAATCTCCCGATTCCCAGTGCGCTCGGTTCGCTGGA
CGCCTACATCGGTGCCGTGCACCAGATCCCGGTGCTGTCGGTCGATGAGGAGCAGGATCTGGCCCGTCGCTTCCGCGACG
AGCTGGACCTGGACGCCGCGCGCGAACTGGTCCATTCCCACCTGCGCTTCGTGGTGCACGTGGCCCGCGGCTACAACGGC
TACGGCCTGCCGCTGGGCGACCTGATCCAGGAAGGCAATATCGGCCTGATGAAGGCGGTCAAGCGCTTCGACCCGGACAT
GGGCGTGCGCCTGGTCAGCTTCGCGGTGCACTGGATCCGTGCCGAGATGCACGAGTTCATCCTGAAGAACTGGCGCATCG
TCAAGGTCGCCACCACCAAGGCGCAGCGCAAGCTGTTCTTCAACCTGCGCAAGTCCAAGACCCGTCTGGGCTGGCTCAAT
GCGTCGGAAGTGACTGCGGTTGCCAAGGACCTGAACGTCTCCGAGCGTGAAGTGATGGAGATGGAGTCGCGGCTGTCCGG
TCGCGATATCGGTTTCGACGCGTCGTCGGACGAAGACGACGATCACGGCCCGCCGTCGCCGGTGAGCTATCTGGTCGCCA
ATGAAGAAGACCCGTCGCAGGCGTACGAGCGTCACGACAGCGAAGACAACCAGCTGCAGCTGCTGCGCGAAGGCATGGCT
GGACTGGACGCGCGCTCGCGCGACATCGTCAAGCGCCGTTGGCTGGATTCCGATTCCAAGGTGACGCTGCAGGAACTGGC
AGACGAATACGGCGTCTCGGCCGAGCGTATCCGCCAGATCGAAGCCAATGCGCTGAAGAAGATGAAGGCGCTGTTCGTCG
CCTGA

Upstream 100 bases:

>100_bases
GGGCTGTAGAACACCGGTGATGCCAAAGACCCGAACGTTCTATAAATGGAATGCTGGGAACTCTTTCTGTACCCGGCAGT
CCAATAGTTCGACTGCTAAG

Downstream 100 bases:

>100_bases
CCCTGTCGGTCTTGCGCGAGTGGTTGTACAGACGGCCCGGTTTTCCGGGCCGTTTGCATATGTGGTGTTACGCGATTGCT
ACTCCGGCGGGCGCGCCACC

Product: RNA polymerase factor sigma-32

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 294; Mature: 294

Protein sequence:

>294_residues
MVPMNQTTSTALVANNLPIPSALGSLDAYIGAVHQIPVLSVDEEQDLARRFRDELDLDAARELVHSHLRFVVHVARGYNG
YGLPLGDLIQEGNIGLMKAVKRFDPDMGVRLVSFAVHWIRAEMHEFILKNWRIVKVATTKAQRKLFFNLRKSKTRLGWLN
ASEVTAVAKDLNVSEREVMEMESRLSGRDIGFDASSDEDDDHGPPSPVSYLVANEEDPSQAYERHDSEDNQLQLLREGMA
GLDARSRDIVKRRWLDSDSKVTLQELADEYGVSAERIRQIEANALKKMKALFVA

Sequences:

>Translated_294_residues
MVPMNQTTSTALVANNLPIPSALGSLDAYIGAVHQIPVLSVDEEQDLARRFRDELDLDAARELVHSHLRFVVHVARGYNG
YGLPLGDLIQEGNIGLMKAVKRFDPDMGVRLVSFAVHWIRAEMHEFILKNWRIVKVATTKAQRKLFFNLRKSKTRLGWLN
ASEVTAVAKDLNVSEREVMEMESRLSGRDIGFDASSDEDDDHGPPSPVSYLVANEEDPSQAYERHDSEDNQLQLLREGMA
GLDARSRDIVKRRWLDSDSKVTLQELADEYGVSAERIRQIEANALKKMKALFVA
>Mature_294_residues
MVPMNQTTSTALVANNLPIPSALGSLDAYIGAVHQIPVLSVDEEQDLARRFRDELDLDAARELVHSHLRFVVHVARGYNG
YGLPLGDLIQEGNIGLMKAVKRFDPDMGVRLVSFAVHWIRAEMHEFILKNWRIVKVATTKAQRKLFFNLRKSKTRLGWLN
ASEVTAVAKDLNVSEREVMEMESRLSGRDIGFDASSDEDDDHGPPSPVSYLVANEEDPSQAYERHDSEDNQLQLLREGMA
GLDARSRDIVKRRWLDSDSKVTLQELADEYGVSAERIRQIEANALKKMKALFVA

Specific function: Sigma factors are initiation factors that promote the attachment of RNA polymerase to specific initiation sites and are then released. This sigma factor is responsible for the expression of heat shock promoters [H]

COG id: COG0568

COG function: function code K; DNA-directed RNA polymerase, sigma subunit (sigma70/sigma32)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the sigma-70 factor family. Sigma-32 subfamily [H]

Homologues:

Organism=Escherichia coli, GI1789871, Length=268, Percent_Identity=60.0746268656716, Blast_Score=324, Evalue=4e-90,
Organism=Escherichia coli, GI1789098, Length=283, Percent_Identity=27.5618374558304, Blast_Score=105, Evalue=4e-24,
Organism=Escherichia coli, GI1789448, Length=257, Percent_Identity=28.4046692607004, Blast_Score=92, Evalue=6e-20,

Paralogues:

None

Copy number: <10 [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR014284
- InterPro:   IPR000943
- InterPro:   IPR009042
- InterPro:   IPR007627
- InterPro:   IPR007630
- InterPro:   IPR013325
- InterPro:   IPR013324
- InterPro:   IPR012759
- InterPro:   IPR011991 [H]

Pfam domain/function: PF00140 Sigma70_r1_2; PF04542 Sigma70_r2; PF04545 Sigma70_r4 [H]

EC number: NA

Molecular weight: Translated: 33110; Mature: 33110

Theoretical pI: Translated: 5.66; Mature: 5.66

Prosite motif: PS00715 SIGMA70_1 ; PS00716 SIGMA70_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
3.1 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MVPMNQTTSTALVANNLPIPSALGSLDAYIGAVHQIPVLSVDEEQDLARRFRDELDLDAA
CCCCCCCCCCEEEECCCCCCHHHHHHHHHHHHHHHCCEEECCCHHHHHHHHHHHCCHHHH
RELVHSHLRFVVHVARGYNGYGLPLGDLIQEGNIGLMKAVKRFDPDMGVRLVSFAVHWIR
HHHHHHHHHHHHHHHHCCCCCCCCHHHHHHCCCHHHHHHHHHCCCCCCHHHHHHHHHHHH
AEMHEFILKNWRIVKVATTKAQRKLFFNLRKSKTRLGWLNASEVTAVAKDLNVSEREVME
HHHHHHHHHCCEEEEEEHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHCCCHHHHHHH
MESRLSGRDIGFDASSDEDDDHGPPSPVSYLVANEEDPSQAYERHDSEDNQLQLLREGMA
HHHHCCCCCCCCCCCCCCCCCCCCCCCCEEEEECCCCHHHHHHHCCCCCHHHHHHHHHHH
GLDARSRDIVKRRWLDSDSKVTLQELADEYGVSAERIRQIEANALKKMKALFVA
CCCCHHHHHHHHHHCCCCCCCHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MVPMNQTTSTALVANNLPIPSALGSLDAYIGAVHQIPVLSVDEEQDLARRFRDELDLDAA
CCCCCCCCCCEEEECCCCCCHHHHHHHHHHHHHHHCCEEECCCHHHHHHHHHHHCCHHHH
RELVHSHLRFVVHVARGYNGYGLPLGDLIQEGNIGLMKAVKRFDPDMGVRLVSFAVHWIR
HHHHHHHHHHHHHHHHCCCCCCCCHHHHHHCCCHHHHHHHHHCCCCCCHHHHHHHHHHHH
AEMHEFILKNWRIVKVATTKAQRKLFFNLRKSKTRLGWLNASEVTAVAKDLNVSEREVME
HHHHHHHHHCCEEEEEEHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHCCCHHHHHHH
MESRLSGRDIGFDASSDEDDDHGPPSPVSYLVANEEDPSQAYERHDSEDNQLQLLREGMA
HHHHCCCCCCCCCCCCCCCCCCCCCCCCEEEEECCCCHHHHHHHCCCCCHHHHHHHHHHH
GLDARSRDIVKRRWLDSDSKVTLQELADEYGVSAERIRQIEANALKKMKALFVA
CCCCHHHHHHHHHHCCCCCCCHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9168128; 10952301 [H]