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The map label for this gene is pdhB [H]

Identifier: 78049335

GI number: 78049335

Start: 4362891

End: 4364654

Strand: Reverse

Name: pdhB [H]

Synonym: XCV3779

Alternate gene names: 78049335

Gene position: 4364654-4362891 (Counterclockwise)

Preceding gene: 78049336

Following gene: 78049334

Centisome position: 84.28

GC content: 67.29

Gene sequence:

>1764_bases
ATGGCCGAAATCAAGGAAGCACTTGTCCCCGATATCGGTGACTACAGCGACGTCCCGGTAATCGAGGTGCTGGTGTCCGT
CGGCGATACGGTCAGCAAGGACCAGAGCCTGGTCACGCTGGAATCTGACAAGGCCACCATGGAAGTGCCTTCGTCGGTGG
CGGGCGTGGTCAAGGAACTCAAGGTCAAGGTCGGCGATTCGCTCTCGCAGGGCGCGCTGGTGGCGTTGATCGAAGTGGCC
GATGCCGGCGCCGAGACCGCCAAGCCCGCCGCCGCCGCACCGGCCAAGGCGGCGCCCGCCGCCGCCCCGGCACCGGCCGC
CAAGGCCGAATCTGCTGCGCCAGCGGCTTCCTCGGACGGCGGCCTGATCGAAGCGCGCGTGCCCGATATCGGCGACTACA
CCGACATCCCGGTGATCGAGGTGCTGGTGGCCGTGGGCGATACCGTCGCCAAGGACCAGAGCCTGGTCACGCTGGAATCG
GACAAGGCCACGATGGAAGTCCCGTCCTCGGCCGCCGGCGTGGTCAAGGAACTCAAGGTCAAGGTCGGCGACACGCTCTC
GCAGGGCAATGTGGTGGCGATCATCGCGGCCAGCGATGGCGGCACCGGTGCGGCGCAGTCGCCGGCCAAGCCCACCACCG
ACACCGCCGAAACCGCGGGCAAGGTCGAGCCGGTTGCCGTCCCGGCCGAGCCGGACAAGCTGGCCCAGCGCGAAATCGCG
CAGGTGCAGGGCGCGCGTTCCGGCGCCGGCACGCAGGCAGCGCAAGGCGGCCAGCCGTCCGCGGGCAACCCGAGCAGCCC
GCCGGTGACCTTCGATGCCGACAGCGTGCTGCCGTCCAAGGTGCCGTACGCCAGCCCGGTGGTGCGCGTGTTCGCACGCG
AACTGGGCGTGGACCTGAACCAGCTCAAGGGCAGCGAAAAGGGCGGCCGCATCACCCGTGAAGACGTGCAGCGGTATGTG
AAGGCTGCACTCAGCGGTGGCGCACCGGCCGCAGCCGGCGCTGCGCCTGCCGGTGGCGGCAACGGCCTGAACCTGCTGGC
CTGGCCGAAGGTGGACTTCAGCAAGTTCGGCGAGACCGAAACCCAGCCGCTGTCGCGCATCAAGAAGATTTCCGGTGCCA
ACCTGGCACGCAACTGGGCAATGATTCCGCACGTCACCCAGTTCGAGTCGGCCGACATCACCGACCTGGAAGCCCTGCGC
GTGGCCCTCAACAAGGAAAACGAGAAGGCCGGCATCAAGCTGACCATGCTCGCCTTCCTGATCAAGGCCAGCGCCGCGGC
GCTGAAGAAGTTCCCCGAGTTCAACGCCTCGCTGGATGCGGCCGGCGAGAACCTCACCCTGAAGAAGTACTTCCACATCG
GGTTTGCGGCCGATACGCCCAACGGCCTGGTGGTGCCGGTGATTCGCGATGTCGACAAGAAGGGCGTGCTGCAGATCGCG
CAGGAAAGCGGCGAGCTGGCCAAGAAGGCGCGCGACGGCAAGCTGGGCCCGGCCGACATGAGCGGCGGCTGCTTCTCGAT
CAGCTCGCTCGGCGGTATCGGCGGCATCGCGTTCACGCCGATCATCAATGCGCCGGAAGTGGCGATCCTGGGCGTGTCCA
AGTCGGCGATGCAGCCGGTGTGGAACGGCAAGGAGTTCGCGCCGAAGCTGATGCTGCCGTTGTCGCTGAGCTACGACCAC
CGCGTCATCGACGGCGCATTGGCAGCCCGTTTCACCACCTACCTCAGCCAGGTGCTGGCCGACATGCGTCGCGTCCTGCT
GTGA

Upstream 100 bases:

>100_bases
TCCCGCCGGGCGCGCGATTTTTTGCACGGCAATGGTTGGCGCTGCGGCCCGCAGCCGGCCACAATAGCGGTTTCCCCATT
CTGTTGCCGGAGTTCCCCGT

Downstream 100 bases:

>100_bases
GGGCCGCGCTGCTGACCATTGCCGTGCTCGGCGTGCTGCCGTGGGCAAACGCGGCCGCACGCGAATGCGAGAGCACGCTC
GGCCGCGGCTGGCCGCCGGC

Product: dihydrolipoamide acetyltransferase

Products: NA

Alternate protein names: Dihydrolipoamide acetyltransferase component of pyruvate dehydrogenase complex; E2 [H]

Number of amino acids: Translated: 587; Mature: 586

Protein sequence:

>587_residues
MAEIKEALVPDIGDYSDVPVIEVLVSVGDTVSKDQSLVTLESDKATMEVPSSVAGVVKELKVKVGDSLSQGALVALIEVA
DAGAETAKPAAAAPAKAAPAAAPAPAAKAESAAPAASSDGGLIEARVPDIGDYTDIPVIEVLVAVGDTVAKDQSLVTLES
DKATMEVPSSAAGVVKELKVKVGDTLSQGNVVAIIAASDGGTGAAQSPAKPTTDTAETAGKVEPVAVPAEPDKLAQREIA
QVQGARSGAGTQAAQGGQPSAGNPSSPPVTFDADSVLPSKVPYASPVVRVFARELGVDLNQLKGSEKGGRITREDVQRYV
KAALSGGAPAAAGAAPAGGGNGLNLLAWPKVDFSKFGETETQPLSRIKKISGANLARNWAMIPHVTQFESADITDLEALR
VALNKENEKAGIKLTMLAFLIKASAAALKKFPEFNASLDAAGENLTLKKYFHIGFAADTPNGLVVPVIRDVDKKGVLQIA
QESGELAKKARDGKLGPADMSGGCFSISSLGGIGGIAFTPIINAPEVAILGVSKSAMQPVWNGKEFAPKLMLPLSLSYDH
RVIDGALAARFTTYLSQVLADMRRVLL

Sequences:

>Translated_587_residues
MAEIKEALVPDIGDYSDVPVIEVLVSVGDTVSKDQSLVTLESDKATMEVPSSVAGVVKELKVKVGDSLSQGALVALIEVA
DAGAETAKPAAAAPAKAAPAAAPAPAAKAESAAPAASSDGGLIEARVPDIGDYTDIPVIEVLVAVGDTVAKDQSLVTLES
DKATMEVPSSAAGVVKELKVKVGDTLSQGNVVAIIAASDGGTGAAQSPAKPTTDTAETAGKVEPVAVPAEPDKLAQREIA
QVQGARSGAGTQAAQGGQPSAGNPSSPPVTFDADSVLPSKVPYASPVVRVFARELGVDLNQLKGSEKGGRITREDVQRYV
KAALSGGAPAAAGAAPAGGGNGLNLLAWPKVDFSKFGETETQPLSRIKKISGANLARNWAMIPHVTQFESADITDLEALR
VALNKENEKAGIKLTMLAFLIKASAAALKKFPEFNASLDAAGENLTLKKYFHIGFAADTPNGLVVPVIRDVDKKGVLQIA
QESGELAKKARDGKLGPADMSGGCFSISSLGGIGGIAFTPIINAPEVAILGVSKSAMQPVWNGKEFAPKLMLPLSLSYDH
RVIDGALAARFTTYLSQVLADMRRVLL
>Mature_586_residues
AEIKEALVPDIGDYSDVPVIEVLVSVGDTVSKDQSLVTLESDKATMEVPSSVAGVVKELKVKVGDSLSQGALVALIEVAD
AGAETAKPAAAAPAKAAPAAAPAPAAKAESAAPAASSDGGLIEARVPDIGDYTDIPVIEVLVAVGDTVAKDQSLVTLESD
KATMEVPSSAAGVVKELKVKVGDTLSQGNVVAIIAASDGGTGAAQSPAKPTTDTAETAGKVEPVAVPAEPDKLAQREIAQ
VQGARSGAGTQAAQGGQPSAGNPSSPPVTFDADSVLPSKVPYASPVVRVFARELGVDLNQLKGSEKGGRITREDVQRYVK
AALSGGAPAAAGAAPAGGGNGLNLLAWPKVDFSKFGETETQPLSRIKKISGANLARNWAMIPHVTQFESADITDLEALRV
ALNKENEKAGIKLTMLAFLIKASAAALKKFPEFNASLDAAGENLTLKKYFHIGFAADTPNGLVVPVIRDVDKKGVLQIAQ
ESGELAKKARDGKLGPADMSGGCFSISSLGGIGGIAFTPIINAPEVAILGVSKSAMQPVWNGKEFAPKLMLPLSLSYDHR
VIDGALAARFTTYLSQVLADMRRVLL

Specific function: The pyruvate dehydrogenase complex catalyzes the overall conversion of pyruvate to acetyl-CoA and CO(2). It contains multiple copies of three enzymatic components:pyruvate dehydrogenase (E1), dihydrolipoamide acetyltransferase (E2) and lipoamide dehydroge

COG id: COG0508

COG function: function code C; Pyruvate/2-oxoglutarate dehydrogenase complex, dihydrolipoamide acyltransferase (E2) component, and related enzymes

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 2 lipoyl-binding domains [H]

Homologues:

Organism=Homo sapiens, GI110671329, Length=481, Percent_Identity=28.0665280665281, Blast_Score=160, Evalue=3e-39,
Organism=Homo sapiens, GI31711992, Length=337, Percent_Identity=33.2344213649852, Blast_Score=145, Evalue=1e-34,
Organism=Homo sapiens, GI19923748, Length=228, Percent_Identity=35.0877192982456, Blast_Score=132, Evalue=7e-31,
Organism=Homo sapiens, GI203098816, Length=303, Percent_Identity=31.3531353135314, Blast_Score=122, Evalue=9e-28,
Organism=Homo sapiens, GI203098753, Length=289, Percent_Identity=31.8339100346021, Blast_Score=119, Evalue=7e-27,
Organism=Homo sapiens, GI260898739, Length=153, Percent_Identity=37.9084967320261, Blast_Score=92, Evalue=1e-18,
Organism=Escherichia coli, GI1786305, Length=308, Percent_Identity=59.0909090909091, Blast_Score=387, Evalue=1e-108,
Organism=Escherichia coli, GI1786946, Length=445, Percent_Identity=30.7865168539326, Blast_Score=179, Evalue=3e-46,
Organism=Caenorhabditis elegans, GI17537937, Length=461, Percent_Identity=27.3318872017354, Blast_Score=147, Evalue=2e-35,
Organism=Caenorhabditis elegans, GI17560088, Length=448, Percent_Identity=29.2410714285714, Blast_Score=134, Evalue=9e-32,
Organism=Caenorhabditis elegans, GI25146366, Length=215, Percent_Identity=40, Blast_Score=130, Evalue=2e-30,
Organism=Caenorhabditis elegans, GI17538894, Length=220, Percent_Identity=34.0909090909091, Blast_Score=112, Evalue=8e-25,
Organism=Saccharomyces cerevisiae, GI6320352, Length=237, Percent_Identity=34.1772151898734, Blast_Score=140, Evalue=4e-34,
Organism=Saccharomyces cerevisiae, GI6324258, Length=335, Percent_Identity=30.1492537313433, Blast_Score=124, Evalue=3e-29,
Organism=Drosophila melanogaster, GI18859875, Length=314, Percent_Identity=33.1210191082803, Blast_Score=155, Evalue=5e-38,
Organism=Drosophila melanogaster, GI24582497, Length=233, Percent_Identity=33.4763948497854, Blast_Score=129, Evalue=5e-30,
Organism=Drosophila melanogaster, GI20129315, Length=227, Percent_Identity=33.920704845815, Blast_Score=129, Evalue=6e-30,
Organism=Drosophila melanogaster, GI24645909, Length=209, Percent_Identity=35.4066985645933, Blast_Score=115, Evalue=9e-26,

Paralogues:

None

Copy number: 1120 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). 912 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 4,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003016
- InterPro:   IPR001078
- InterPro:   IPR006256
- InterPro:   IPR000089
- InterPro:   IPR023213
- InterPro:   IPR004167
- InterPro:   IPR011053 [H]

Pfam domain/function: PF00198 2-oxoacid_dh; PF00364 Biotin_lipoyl; PF02817 E3_binding [H]

EC number: =2.3.1.12 [H]

Molecular weight: Translated: 60140; Mature: 60009

Theoretical pI: Translated: 4.94; Mature: 4.94

Prosite motif: PS50968 BIOTINYL_LIPOYL ; PS00189 LIPOYL

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
1.5 %Met     (Translated Protein)
1.7 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
1.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAEIKEALVPDIGDYSDVPVIEVLVSVGDTVSKDQSLVTLESDKATMEVPSSVAGVVKEL
CCCHHHHHCCCCCCCCCCHHHHHHHHHCCCCCCCCCEEEEECCCCCHHCCHHHHHHHHHH
KVKVGDSLSQGALVALIEVADAGAETAKPAAAAPAKAAPAAAPAPAAKAESAAPAASSDG
HHHHCCCCCCCCEEEEEEHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCC
GLIEARVPDIGDYTDIPVIEVLVAVGDTVAKDQSLVTLESDKATMEVPSSAAGVVKELKV
CEEEEECCCCCCCCCCHHHHHHHHHCCHHHCCCCEEEEECCCCEEECCCHHHHHHHHHHH
KVGDTLSQGNVVAIIAASDGGTGAAQSPAKPTTDTAETAGKVEPVAVPAEPDKLAQREIA
HHCCCCCCCCEEEEEEECCCCCCCCCCCCCCCCCHHHHCCCCCEEECCCCHHHHHHHHHH
QVQGARSGAGTQAAQGGQPSAGNPSSPPVTFDADSVLPSKVPYASPVVRVFARELGVDLN
HHHCCCCCCCCCCCCCCCCCCCCCCCCCEEECCCCCCCCCCCCCHHHHHHHHHHHCCCHH
QLKGSEKGGRITREDVQRYVKAALSGGAPAAAGAAPAGGGNGLNLLAWPKVDFSKFGETE
HHCCCCCCCCCCHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCEEEEECCCCHHHCCCCC
TQPLSRIKKISGANLARNWAMIPHVTQFESADITDLEALRVALNKENEKAGIKLTMLAFL
HHHHHHHHHHCCCCHHHCCCCCCCCCCCCCCCCCHHHHHHHHHCCCCCCCCCHHHHHHHH
IKASAAALKKFPEFNASLDAAGENLTLKKYFHIGFAADTPNGLVVPVIRDVDKKGVLQIA
HHHHHHHHHHCCCCCCCCCCCCCCEEEEEEEEEEEECCCCCCEEEHHHHCCCHHHHHHHH
QESGELAKKARDGKLGPADMSGGCFSISSLGGIGGIAFTPIINAPEVAILGVSKSAMQPV
HHHHHHHHHHCCCCCCCCCCCCCEEEHHHCCCCCCEEECCCCCCCCEEEEECCHHHHCCC
WNGKEFAPKLMLPLSLSYDHRVIDGALAARFTTYLSQVLADMRRVLL
CCCHHCCCEEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHC
>Mature Secondary Structure 
AEIKEALVPDIGDYSDVPVIEVLVSVGDTVSKDQSLVTLESDKATMEVPSSVAGVVKEL
CCHHHHHCCCCCCCCCCHHHHHHHHHCCCCCCCCCEEEEECCCCCHHCCHHHHHHHHHH
KVKVGDSLSQGALVALIEVADAGAETAKPAAAAPAKAAPAAAPAPAAKAESAAPAASSDG
HHHHCCCCCCCCEEEEEEHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCC
GLIEARVPDIGDYTDIPVIEVLVAVGDTVAKDQSLVTLESDKATMEVPSSAAGVVKELKV
CEEEEECCCCCCCCCCHHHHHHHHHCCHHHCCCCEEEEECCCCEEECCCHHHHHHHHHHH
KVGDTLSQGNVVAIIAASDGGTGAAQSPAKPTTDTAETAGKVEPVAVPAEPDKLAQREIA
HHCCCCCCCCEEEEEEECCCCCCCCCCCCCCCCCHHHHCCCCCEEECCCCHHHHHHHHHH
QVQGARSGAGTQAAQGGQPSAGNPSSPPVTFDADSVLPSKVPYASPVVRVFARELGVDLN
HHHCCCCCCCCCCCCCCCCCCCCCCCCCEEECCCCCCCCCCCCCHHHHHHHHHHHCCCHH
QLKGSEKGGRITREDVQRYVKAALSGGAPAAAGAAPAGGGNGLNLLAWPKVDFSKFGETE
HHCCCCCCCCCCHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCEEEEECCCCHHHCCCCC
TQPLSRIKKISGANLARNWAMIPHVTQFESADITDLEALRVALNKENEKAGIKLTMLAFL
HHHHHHHHHHCCCCHHHCCCCCCCCCCCCCCCCCHHHHHHHHHCCCCCCCCCHHHHHHHH
IKASAAALKKFPEFNASLDAAGENLTLKKYFHIGFAADTPNGLVVPVIRDVDKKGVLQIA
HHHHHHHHHHCCCCCCCCCCCCCCEEEEEEEEEEEECCCCCCEEEHHHHCCCHHHHHHHH
QESGELAKKARDGKLGPADMSGGCFSISSLGGIGGIAFTPIINAPEVAILGVSKSAMQPV
HHHHHHHHHHCCCCCCCCCCCCCEEEHHHCCCCCCEEECCCCCCCCEEEEECCHHHHCCC
WNGKEFAPKLMLPLSLSYDHRVIDGALAARFTTYLSQVLADMRRVLL
CCCHHCCCEEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8021225 [H]