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The map label for this gene is lpdA [H]

Identifier: 78049333

GI number: 78049333

Start: 4360407

End: 4362230

Strand: Reverse

Name: lpdA [H]

Synonym: XCV3777

Alternate gene names: 78049333

Gene position: 4362230-4360407 (Counterclockwise)

Preceding gene: 78049334

Following gene: 78049332

Centisome position: 84.24

GC content: 66.56

Gene sequence:

>1824_bases
ATGGCGGTCATTGAAATCAAGGTTCCCGACATCGGCGATTACAGCGATGTCCCCGTCATCGAAGTGCTGGTCGCCGTCGG
CGACAGCGTGGCCAAGGACCAGGGCCTGGTAACGCTGGAATCGGACAAGGCCACGCTGGAAGTGCCGTCCTCGGCGGCCG
GTGTGGTCAAGGAACTCAAGGTCAAGGTCGGCGACACCCTGTCCGAAGGCGCGCTCGTGCTGCTGCTCGAAACCGAAGGC
GAGGCTGCCGCGCCGGCCAAGGCCGAACCCAAGGCTGCCCCGGCCGCTGCAGCGCCTGCCGCCGCACCGGGCAGCAAGCC
GCCGGTGACGCCGTCGCACCGTGCCCCGGCCGAGCCGGCCGCGCCCAAGCCGGCGCTGGCCAGCGGCAAGCCGGCCGACA
TCGAATGCAAGATGGTGGTGCTCGGCGCCGGCCCCGGCGGCTACACCGCTGCCTTCCGCGCGGCCGATCTGGGCCTGGAG
ACGGTGCTGATCGAGCGCTACGCCAGCCTGGGCGGTGTCTGTCTCAACGTCGGCTGCATCCCGTCCAAGGCGCTGCTGCA
TGCCGCCGCGGTGATCGATGAAGTGGCCCATGCCGGCGACTTCGGCGTGGACTTCGGCCAGCCCAAGATCGCCCTGGACA
AGCTGCGCGAGTACAAGGAAAAAGTGGTCGGCAAGCTCACCGGCGGCCTGGCCAGCATGGCCAAGCAGCGCAAGGTGCGC
ACCGTCACCGGCGTGGCCAGCTTCGTCTCGCCCAACGAGCTGGAAATCGTCGGCGACGACGGCAAGACCCAACTGCTGCG
CTTTGAGCACTGCATCATCGCCGCCGGCTCGCAGGCGGTGAAGCTGCCCAACTTCCCATGGGACGACAAGCGCGTGATGG
ACTCCACCGACGCGCTGGAGTTGCACGACATTCCCAAGACCCTGCTGGTGGTCGGCGGCGGCATCATCGGCCTGGAAATG
GCCACGGTGTACAGCGCGCTCGGCAGCAAGGTCACCGTGGTGGAGTTCATGGACCAGCTGATGCCGGGCGCCGACAAGGA
CCTGGTCAAGCCGCTGGCCGATCGCCTGAAAAAGCAGGGCGTCGAGGTTCATCTCAAGACCAAGGCCACCGACGTCAAGG
CCGACAAGGCCGGCATCACCGTGTCCTTCGAAGCGGCTGTGGAAGGCGAAAAGCCCGGCCTGCAGGCCACCACGTACGAC
CGCGTGCTGGTTGCCGTGGGCCGTAGCCCGAACGGCAAGAAGATCGGTGCGGAAAAGGCCGGCGTCACCGTCACCGAGCG
CGGCTTCATTCCGGTCGATCGGCAGATGCGCACCAACGTGCCGCACATCTTCGCCATCGGCGACATCGTCGGTAACCCGA
TGCTGGCGCACAAGGCCACCCACGAAGGCAAGCTGGCTGCCGAAGTGGCTGCCGGCGAGAAGAAGGAATGGGTGGCGCGG
GTGATCCCGTCGGTGGCCTACACCAACCCGGAAATCGCCTGGGTCGGCGTCACCGAGACCGAAGCCAAGGCCAAGGGCCT
GAAGGTGGGCGTGGCCAAGTTCCCGTGGGCCGCCAGCGGCCGCGCGATCGGCATCGGCCGTACCGAAGGTTTCACCAAGT
TGATCTTCGATGAACAGACGCATCGCGTGATCGGTGGCGCCATCGTCGGCGTGCATGCCGGCGACCTGCTGGCTGAAATT
GGCCTGGCGATCGAAATGGGTGCCGAGGCCGAAGACATCGGCCACACCATCCACGCGCACCCGACCCTGAGCGAGTCGGT
CGGCATGGCCGCCGAGGTCTACGACGGCACCATCACCGATCTGTACATTCCCAAGAAGAAGTGA

Upstream 100 bases:

>100_bases
GAAAAGAAGCGCGACAAGCGCTGGACCCAAATCGAATCGTCGCTGGATGAACTCCAGCAGACGCTTGCCGGCACGGCCGG
TTGAGCTACAGGAGAAGCAA

Downstream 100 bases:

>100_bases
CGCCGCTCCGCACGACCGTGCCGCCACGCACGGAGGTCGCCGTGGGGCAGGGCAATCTGTGTCCCGAACTGCAGGCGCTG
CTGCAGCGCGAATTGGCCGG

Product: dihydrolipoamide dehydrogenase

Products: NA

Alternate protein names: Dihydrolipoamide dehydrogenase; E3 component of 2-oxoglutarate dehydrogenase complex [H]

Number of amino acids: Translated: 607; Mature: 606

Protein sequence:

>607_residues
MAVIEIKVPDIGDYSDVPVIEVLVAVGDSVAKDQGLVTLESDKATLEVPSSAAGVVKELKVKVGDTLSEGALVLLLETEG
EAAAPAKAEPKAAPAAAAPAAAPGSKPPVTPSHRAPAEPAAPKPALASGKPADIECKMVVLGAGPGGYTAAFRAADLGLE
TVLIERYASLGGVCLNVGCIPSKALLHAAAVIDEVAHAGDFGVDFGQPKIALDKLREYKEKVVGKLTGGLASMAKQRKVR
TVTGVASFVSPNELEIVGDDGKTQLLRFEHCIIAAGSQAVKLPNFPWDDKRVMDSTDALELHDIPKTLLVVGGGIIGLEM
ATVYSALGSKVTVVEFMDQLMPGADKDLVKPLADRLKKQGVEVHLKTKATDVKADKAGITVSFEAAVEGEKPGLQATTYD
RVLVAVGRSPNGKKIGAEKAGVTVTERGFIPVDRQMRTNVPHIFAIGDIVGNPMLAHKATHEGKLAAEVAAGEKKEWVAR
VIPSVAYTNPEIAWVGVTETEAKAKGLKVGVAKFPWAASGRAIGIGRTEGFTKLIFDEQTHRVIGGAIVGVHAGDLLAEI
GLAIEMGAEAEDIGHTIHAHPTLSESVGMAAEVYDGTITDLYIPKKK

Sequences:

>Translated_607_residues
MAVIEIKVPDIGDYSDVPVIEVLVAVGDSVAKDQGLVTLESDKATLEVPSSAAGVVKELKVKVGDTLSEGALVLLLETEG
EAAAPAKAEPKAAPAAAAPAAAPGSKPPVTPSHRAPAEPAAPKPALASGKPADIECKMVVLGAGPGGYTAAFRAADLGLE
TVLIERYASLGGVCLNVGCIPSKALLHAAAVIDEVAHAGDFGVDFGQPKIALDKLREYKEKVVGKLTGGLASMAKQRKVR
TVTGVASFVSPNELEIVGDDGKTQLLRFEHCIIAAGSQAVKLPNFPWDDKRVMDSTDALELHDIPKTLLVVGGGIIGLEM
ATVYSALGSKVTVVEFMDQLMPGADKDLVKPLADRLKKQGVEVHLKTKATDVKADKAGITVSFEAAVEGEKPGLQATTYD
RVLVAVGRSPNGKKIGAEKAGVTVTERGFIPVDRQMRTNVPHIFAIGDIVGNPMLAHKATHEGKLAAEVAAGEKKEWVAR
VIPSVAYTNPEIAWVGVTETEAKAKGLKVGVAKFPWAASGRAIGIGRTEGFTKLIFDEQTHRVIGGAIVGVHAGDLLAEI
GLAIEMGAEAEDIGHTIHAHPTLSESVGMAAEVYDGTITDLYIPKKK
>Mature_606_residues
AVIEIKVPDIGDYSDVPVIEVLVAVGDSVAKDQGLVTLESDKATLEVPSSAAGVVKELKVKVGDTLSEGALVLLLETEGE
AAAPAKAEPKAAPAAAAPAAAPGSKPPVTPSHRAPAEPAAPKPALASGKPADIECKMVVLGAGPGGYTAAFRAADLGLET
VLIERYASLGGVCLNVGCIPSKALLHAAAVIDEVAHAGDFGVDFGQPKIALDKLREYKEKVVGKLTGGLASMAKQRKVRT
VTGVASFVSPNELEIVGDDGKTQLLRFEHCIIAAGSQAVKLPNFPWDDKRVMDSTDALELHDIPKTLLVVGGGIIGLEMA
TVYSALGSKVTVVEFMDQLMPGADKDLVKPLADRLKKQGVEVHLKTKATDVKADKAGITVSFEAAVEGEKPGLQATTYDR
VLVAVGRSPNGKKIGAEKAGVTVTERGFIPVDRQMRTNVPHIFAIGDIVGNPMLAHKATHEGKLAAEVAAGEKKEWVARV
IPSVAYTNPEIAWVGVTETEAKAKGLKVGVAKFPWAASGRAIGIGRTEGFTKLIFDEQTHRVIGGAIVGVHAGDLLAEIG
LAIEMGAEAEDIGHTIHAHPTLSESVGMAAEVYDGTITDLYIPKKK

Specific function: The branched-chain alpha-keto dehydrogenase complex catalyzes the overall conversion of alpha-keto acids to acyl-CoA and CO(2). It contains multiple copies of 3 enzymatic components:branched-chain alpha-keto acid decarboxylase (E1), lipoamide acyltransfer

COG id: COG1249

COG function: function code C; Pyruvate/2-oxoglutarate dehydrogenase complex, dihydrolipoamide dehydrogenase (E3) component, and related enzymes

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-I pyridine nucleotide-disulfide oxidoreductase family [H]

Homologues:

Organism=Homo sapiens, GI91199540, Length=458, Percent_Identity=41.9213973799127, Blast_Score=347, Evalue=2e-95,
Organism=Homo sapiens, GI50301238, Length=450, Percent_Identity=28.2222222222222, Blast_Score=166, Evalue=5e-41,
Organism=Homo sapiens, GI148277071, Length=435, Percent_Identity=27.5862068965517, Blast_Score=150, Evalue=4e-36,
Organism=Homo sapiens, GI148277065, Length=435, Percent_Identity=27.5862068965517, Blast_Score=150, Evalue=5e-36,
Organism=Homo sapiens, GI33519430, Length=435, Percent_Identity=27.5862068965517, Blast_Score=149, Evalue=5e-36,
Organism=Homo sapiens, GI33519428, Length=435, Percent_Identity=27.5862068965517, Blast_Score=149, Evalue=5e-36,
Organism=Homo sapiens, GI33519426, Length=435, Percent_Identity=27.5862068965517, Blast_Score=149, Evalue=5e-36,
Organism=Homo sapiens, GI291045266, Length=432, Percent_Identity=28.2407407407407, Blast_Score=142, Evalue=7e-34,
Organism=Homo sapiens, GI22035672, Length=437, Percent_Identity=29.2906178489703, Blast_Score=139, Evalue=8e-33,
Organism=Homo sapiens, GI291045268, Length=422, Percent_Identity=26.7772511848341, Blast_Score=126, Evalue=8e-29,
Organism=Escherichia coli, GI1786307, Length=475, Percent_Identity=61.2631578947368, Blast_Score=575, Evalue=1e-165,
Organism=Escherichia coli, GI87082354, Length=478, Percent_Identity=28.6610878661088, Blast_Score=186, Evalue=4e-48,
Organism=Escherichia coli, GI87081717, Length=456, Percent_Identity=28.5087719298246, Blast_Score=172, Evalue=5e-44,
Organism=Escherichia coli, GI1789915, Length=440, Percent_Identity=28.1818181818182, Blast_Score=152, Evalue=5e-38,
Organism=Escherichia coli, GI1786305, Length=78, Percent_Identity=56.4102564102564, Blast_Score=84, Evalue=4e-17,
Organism=Caenorhabditis elegans, GI32565766, Length=465, Percent_Identity=41.2903225806452, Blast_Score=349, Evalue=3e-96,
Organism=Caenorhabditis elegans, GI17557007, Length=472, Percent_Identity=29.4491525423729, Blast_Score=150, Evalue=2e-36,
Organism=Caenorhabditis elegans, GI71983429, Length=442, Percent_Identity=27.3755656108597, Blast_Score=131, Evalue=1e-30,
Organism=Caenorhabditis elegans, GI71983419, Length=442, Percent_Identity=27.6018099547511, Blast_Score=130, Evalue=2e-30,
Organism=Caenorhabditis elegans, GI71982272, Length=442, Percent_Identity=25.7918552036199, Blast_Score=124, Evalue=1e-28,
Organism=Saccharomyces cerevisiae, GI6321091, Length=474, Percent_Identity=41.7721518987342, Blast_Score=326, Evalue=7e-90,
Organism=Saccharomyces cerevisiae, GI6325240, Length=483, Percent_Identity=28.9855072463768, Blast_Score=186, Evalue=1e-47,
Organism=Saccharomyces cerevisiae, GI6325166, Length=459, Percent_Identity=29.1938997821351, Blast_Score=170, Evalue=5e-43,
Organism=Drosophila melanogaster, GI21358499, Length=457, Percent_Identity=43.1072210065646, Blast_Score=363, Evalue=1e-100,
Organism=Drosophila melanogaster, GI24640551, Length=513, Percent_Identity=27.2904483430799, Blast_Score=122, Evalue=8e-28,
Organism=Drosophila melanogaster, GI24640553, Length=487, Percent_Identity=26.694045174538, Blast_Score=118, Evalue=1e-26,
Organism=Drosophila melanogaster, GI24640549, Length=431, Percent_Identity=27.8422273781903, Blast_Score=118, Evalue=1e-26,
Organism=Drosophila melanogaster, GI17737741, Length=483, Percent_Identity=25.6728778467909, Blast_Score=107, Evalue=3e-23,

Paralogues:

None

Copy number: 380 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 160 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 1880 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). 6,000 Molecules/Cell In: Glucose minimal

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR016156
- InterPro:   IPR013027
- InterPro:   IPR006258
- InterPro:   IPR004099
- InterPro:   IPR012999
- InterPro:   IPR001327 [H]

Pfam domain/function: PF00070 Pyr_redox; PF07992 Pyr_redox_2; PF02852 Pyr_redox_dim [H]

EC number: =1.8.1.4 [H]

Molecular weight: Translated: 63198; Mature: 63067

Theoretical pI: Translated: 6.06; Mature: 6.06

Prosite motif: PS00076 PYRIDINE_REDOX_1 ; PS50968 BIOTINYL_LIPOYL ; PS00189 LIPOYL

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
2.5 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
2.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAVIEIKVPDIGDYSDVPVIEVLVAVGDSVAKDQGLVTLESDKATLEVPSSAAGVVKELK
CEEEEEECCCCCCCCCCHHHHHHHHHCCCHHCCCCCEEEECCCCEEECCCHHHHHHHHHH
VKVGDTLSEGALVLLLETEGEAAAPAKAEPKAAPAAAAPAAAPGSKPPVTPSHRAPAEPA
HHHCCCCCCCEEEEEEECCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCC
APKPALASGKPADIECKMVVLGAGPGGYTAAFRAADLGLETVLIERYASLGGVCLNVGCI
CCCCCCCCCCCCCEEEEEEEEECCCCCCHHHHHHHHCCHHHHHHHHHHHCCCEEEEECCC
PSKALLHAAAVIDEVAHAGDFGVDFGQPKIALDKLREYKEKVVGKLTGGLASMAKQRKVR
CHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
TVTGVASFVSPNELEIVGDDGKTQLLRFEHCIIAAGSQAVKLPNFPWDDKRVMDSTDALE
HHHHHHHHCCCCEEEEECCCCCEEEEEEEEEEEECCCCEEECCCCCCCCCHHCCCCCCEE
LHDIPKTLLVVGGGIIGLEMATVYSALGSKVTVVEFMDQLMPGADKDLVKPLADRLKKQG
ECCCCCEEEHCCCCHHHHHHHHHHHHHCCCEEHHHHHHHHCCCCCHHHHHHHHHHHHHCC
VEVHLKTKATDVKADKAGITVSFEAAVEGEKPGLQATTYDRVLVAVGRSPNGKKIGAEKA
CEEEEEECCCCCCCCCCCEEEEEEEEECCCCCCCCEECCCEEEEEECCCCCCCCCCCCCC
GVTVTERGFIPVDRQMRTNVPHIFAIGDIVGNPMLAHKATHEGKLAAEVAAGEKKEWVAR
CEEEEECCCCCCCHHHHCCCCEEEEEHHHHCCCHHEECCCCCCCEEEHHHCCCHHHHHHH
VIPSVAYTNPEIAWVGVTETEAKAKGLKVGVAKFPWAASGRAIGIGRTEGFTKLIFDEQT
HHHHHHCCCCCEEEEECCCCHHHHCCEEEEHEECCCCCCCCEEEECCCCCCEEEEECCCH
HRVIGGAIVGVHAGDLLAEIGLAIEMGAEAEDIGHTIHAHPTLSESVGMAAEVYDGTITD
HHEECEEEEEEEHHHHHHHHCCEEECCCCHHHHCCEEECCCCCHHHHCCEEEECCCCEEE
LYIPKKK
EEECCCC
>Mature Secondary Structure 
AVIEIKVPDIGDYSDVPVIEVLVAVGDSVAKDQGLVTLESDKATLEVPSSAAGVVKELK
EEEEEECCCCCCCCCCHHHHHHHHHCCCHHCCCCCEEEECCCCEEECCCHHHHHHHHHH
VKVGDTLSEGALVLLLETEGEAAAPAKAEPKAAPAAAAPAAAPGSKPPVTPSHRAPAEPA
HHHCCCCCCCEEEEEEECCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCC
APKPALASGKPADIECKMVVLGAGPGGYTAAFRAADLGLETVLIERYASLGGVCLNVGCI
CCCCCCCCCCCCCEEEEEEEEECCCCCCHHHHHHHHCCHHHHHHHHHHHCCCEEEEECCC
PSKALLHAAAVIDEVAHAGDFGVDFGQPKIALDKLREYKEKVVGKLTGGLASMAKQRKVR
CHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
TVTGVASFVSPNELEIVGDDGKTQLLRFEHCIIAAGSQAVKLPNFPWDDKRVMDSTDALE
HHHHHHHHCCCCEEEEECCCCCEEEEEEEEEEEECCCCEEECCCCCCCCCHHCCCCCCEE
LHDIPKTLLVVGGGIIGLEMATVYSALGSKVTVVEFMDQLMPGADKDLVKPLADRLKKQG
ECCCCCEEEHCCCCHHHHHHHHHHHHHCCCEEHHHHHHHHCCCCCHHHHHHHHHHHHHCC
VEVHLKTKATDVKADKAGITVSFEAAVEGEKPGLQATTYDRVLVAVGRSPNGKKIGAEKA
CEEEEEECCCCCCCCCCCEEEEEEEEECCCCCCCCEECCCEEEEEECCCCCCCCCCCCCC
GVTVTERGFIPVDRQMRTNVPHIFAIGDIVGNPMLAHKATHEGKLAAEVAAGEKKEWVAR
CEEEEECCCCCCCHHHHCCCCEEEEEHHHHCCCHHEECCCCCCCEEEHHHCCCHHHHHHH
VIPSVAYTNPEIAWVGVTETEAKAKGLKVGVAKFPWAASGRAIGIGRTEGFTKLIFDEQT
HHHHHHCCCCCEEEEECCCCHHHHCCEEEEHEECCCCCCCCEEEECCCCCCEEEEECCCH
HRVIGGAIVGVHAGDLLAEIGLAIEMGAEAEDIGHTIHAHPTLSESVGMAAEVYDGTITD
HHEECEEEEEEEHHHHHHHHCCEEECCCCHHHHCCEEECCCCCHHHHCCEEEECCCCEEE
LYIPKKK
EEECCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 10952301 [H]