Click here to switch to the map view.

The map label for this gene is def [H]

Identifier: 78049192

GI number: 78049192

Start: 4182922

End: 4183437

Strand: Reverse

Name: def [H]

Synonym: XCV3636

Alternate gene names: 78049192

Gene position: 4183437-4182922 (Counterclockwise)

Preceding gene: 78049193

Following gene: 78049190

Centisome position: 80.79

GC content: 61.24

Gene sequence:

>516_bases
ATGATTCGCGAAATTATCCGCATGGGCGACAAGCGCCTGCTACGCGTGGCACCACCGGTCAGCAACCTGGGCAGCGCCGA
GTTGCACACGCTGGTGGCCGATATGTTCGAAACCATGGACGCTGCGCGTGGCGTCGGTCTGGCAGCACCGCAGATCGCGG
TGGATCTGCAATTGATGGTGTTCGGTTTCGACGCCAGCGAGCGCTATCCGGAAGCGCCCGCAGTGCCGCGCACGGCCCTG
GCCAACGCGCAGATCGAACCGCTGTCGGAAGAAATGGAAAACGGCTGGGAAGGCTGCCTGTCGATTCCCGGCCTGCGCGC
AGTCATTCCACGCTATCGCTTCATTCGCTACCGCGGCTTCGCACCCGACGGCACTCCGATCGAGCGCGATGCCGAAGGTT
TCCACGCACGCGTGGTGCAACACGAATACGACCATCTGGTCGGTCGCCTGTATCCCTCGCGCATCGAAAATTTCGACACA
TTCGGTTTCGAAGACGTGTTGTCTTACGACCTGTAG

Upstream 100 bases:

>100_bases
TCGTACACTGCAGCGCCTGCGGACTATTGTACCGCCCGGCGCTTCGCTACCGGGTGGCAGCCGCCCTAGAATCCACGCCA
CGATCGTCAGGAGCACCCGC

Downstream 100 bases:

>100_bases
CAGGTCCTTCGTTGTGTCCAATGCGGCGTGGCTGCCGCCATGGACGGCAGCCACGTCCTGGACTGCGCCGCATTCAAGGC
AATCATCAGCACGCTGCCTG

Product: peptide deformylase

Products: NA

Alternate protein names: PDF 1; Polypeptide deformylase 1 [H]

Number of amino acids: Translated: 171; Mature: 171

Protein sequence:

>171_residues
MIREIIRMGDKRLLRVAPPVSNLGSAELHTLVADMFETMDAARGVGLAAPQIAVDLQLMVFGFDASERYPEAPAVPRTAL
ANAQIEPLSEEMENGWEGCLSIPGLRAVIPRYRFIRYRGFAPDGTPIERDAEGFHARVVQHEYDHLVGRLYPSRIENFDT
FGFEDVLSYDL

Sequences:

>Translated_171_residues
MIREIIRMGDKRLLRVAPPVSNLGSAELHTLVADMFETMDAARGVGLAAPQIAVDLQLMVFGFDASERYPEAPAVPRTAL
ANAQIEPLSEEMENGWEGCLSIPGLRAVIPRYRFIRYRGFAPDGTPIERDAEGFHARVVQHEYDHLVGRLYPSRIENFDT
FGFEDVLSYDL
>Mature_171_residues
MIREIIRMGDKRLLRVAPPVSNLGSAELHTLVADMFETMDAARGVGLAAPQIAVDLQLMVFGFDASERYPEAPAVPRTAL
ANAQIEPLSEEMENGWEGCLSIPGLRAVIPRYRFIRYRGFAPDGTPIERDAEGFHARVVQHEYDHLVGRLYPSRIENFDT
FGFEDVLSYDL

Specific function: Removes the formyl group from the N-terminal Met of newly synthesized proteins. Requires at least a dipeptide for an efficient rate of reaction. N-terminal L-methionine is a prerequisite for activity but the enzyme has broad specificity at other positions

COG id: COG0242

COG function: function code J; N-formylmethionyl-tRNA deformylase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the polypeptide deformylase family [H]

Homologues:

Organism=Homo sapiens, GI11641243, Length=174, Percent_Identity=31.6091954022989, Blast_Score=83, Evalue=1e-16,
Organism=Escherichia coli, GI1789682, Length=149, Percent_Identity=42.2818791946309, Blast_Score=105, Evalue=1e-24,
Organism=Drosophila melanogaster, GI24645728, Length=177, Percent_Identity=29.9435028248588, Blast_Score=90, Evalue=7e-19,
Organism=Drosophila melanogaster, GI24645726, Length=173, Percent_Identity=29.4797687861272, Blast_Score=69, Evalue=1e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000181 [H]

Pfam domain/function: PF01327 Pep_deformylase [H]

EC number: =3.5.1.88 [H]

Molecular weight: Translated: 19179; Mature: 19179

Theoretical pI: Translated: 4.65; Mature: 4.65

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
3.5 %Met     (Translated Protein)
4.1 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
3.5 %Met     (Mature Protein)
4.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIREIIRMGDKRLLRVAPPVSNLGSAELHTLVADMFETMDAARGVGLAAPQIAVDLQLMV
CHHHHHHCCCHHEEEECCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHEEEEEE
FGFDASERYPEAPAVPRTALANAQIEPLSEEMENGWEGCLSIPGLRAVIPRYRFIRYRGF
EECCCCCCCCCCCCCCCHHHCCCCCCCHHHHHHHHHHHHHCCCCHHHHHHHHHHHEECCC
APDGTPIERDAEGFHARVVQHEYDHLVGRLYPSRIENFDTFGFEDVLSYDL
CCCCCCCCCCCCHHHHHHHHHHHHHHHHHHCHHHHCCCCCCCHHHHHHCCC
>Mature Secondary Structure
MIREIIRMGDKRLLRVAPPVSNLGSAELHTLVADMFETMDAARGVGLAAPQIAVDLQLMV
CHHHHHHCCCHHEEEECCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHEEEEEE
FGFDASERYPEAPAVPRTALANAQIEPLSEEMENGWEGCLSIPGLRAVIPRYRFIRYRGF
EECCCCCCCCCCCCCCCHHHCCCCCCCHHHHHHHHHHHHHCCCCHHHHHHHHHHHEECCC
APDGTPIERDAEGFHARVVQHEYDHLVGRLYPSRIENFDTFGFEDVLSYDL
CCCCCCCCCCCCHHHHHHHHHHHHHHHHHHCHHHHCCCCCCCHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12024217 [H]