Click here to switch to the map view.

The map label for this gene is leuB [H]

Identifier: 78049140

GI number: 78049140

Start: 4114592

End: 4115665

Strand: Reverse

Name: leuB [H]

Synonym: XCV3584

Alternate gene names: 78049140

Gene position: 4115665-4114592 (Counterclockwise)

Preceding gene: 78049141

Following gene: 78049134

Centisome position: 79.48

GC content: 63.87

Gene sequence:

>1074_bases
ATGAGCAAGCAGATTCTGATTCTTCCCGGCGACGGGATCGGCCCGGAGATCATGGCCGAAGCGGTCAAGGTATTGAAGCG
CATCGACGCGCAACACGGTCTGGGTTTCGAGCTGGTGTATGACGAGCTGGGCGGCGCTGCGTACGACAAATACGGCAGCC
CGTTGGCCGATGAAACACTGGAGCGCGCGCGCGCCGCCGATGCGGTGTTGCTGGGCGCGGTGGGTGGCCCGCAGTGGGAC
ACCATCGACCCGTCGCTGCGCCCGGAGCGCGGTTTGCTCAAGATCCGCTCGCAGCTGGGCTTGTTCGCCAACCTGCGGCC
GGCATTGCTGTATCCGCAGCTGGCCGATGCGTCCACGCTCAAGCCCGAGGTGGTGGCCGGGCTGGATCTGCTGATCCTGC
GCGAGCTCACCGGCGGCATCTATTTCGGCCAGCCGCGTGGTAACCGCACCTTGGACAACGGCGAACGCCAGGCGTACGAC
ACCTTGCCGTATAGCGAGAGCGAGATTCGTCGCATCGCCAAGGCAGGTTTTGAGATGGCACGCCTGCGCGGGAAGAAGTT
GTGTTCGGTCGACAAGGCCAACGTGCTGGCATCGAGCCAGCTATGGCGTGCAGTGGTCGAGGAAGTGGCCAAGGACTACC
CGGATATCGCGCTGTCGCATATGTACGTGGACAACGCGGCGATGCAGTTGGTGCGCGCGCCCAAGCAGTTCGATGTGATC
GTGACCGACAACATGTTCGGTGACATTCTCTCCGACCAGGCATCCATGCTCACCGGTTCCATCGGCATGCTGCCGTCGGC
CTCGCTGGATGCCAACAGCAAAGGCATGTACGAGCCGTGCCATGGTTCGGCGCCGGACATCGCCGGCAAGGGCATTGCCA
ATCCGCTGGCCACGATCCTGTCGGTGGCGATGATGTTGCGCTACACCTTCGCGCAGGCCGATGCCGCAGACGCCATCGAG
CGCGCCGTGGGCAAGGTCCTCGATCAAGGCCTGCGCACTGCCGACATCTGGTCGGAAGGCACCACGAAGGTGGGCACCGT
GGCAATGGGCGATGCGGTGGTGGCTGCGCTGTAA

Upstream 100 bases:

>100_bases
GCTGCAGTGAGGTTGAGCCGCACGTGCGTGTGTCGATCGTGCCGCGTGCGTGTATTGCATTGAATTCAATGACGCTGCGG
CGTCACTTCACGAGGGTGGC

Downstream 100 bases:

>100_bases
AAGCGCTCGCAGTCCCGGAGCGTTGTAGTGCGCGGGAATGGTCGCCAGCGCAATAACGCATCCGTGTCCTCAGCTGCCGA
CAGCAGCCGGTGGTACCTGC

Product: 3-isopropylmalate dehydrogenase

Products: NA

Alternate protein names: 3-IPM-DH; Beta-IPM dehydrogenase; IMDH [H]

Number of amino acids: Translated: 357; Mature: 356

Protein sequence:

>357_residues
MSKQILILPGDGIGPEIMAEAVKVLKRIDAQHGLGFELVYDELGGAAYDKYGSPLADETLERARAADAVLLGAVGGPQWD
TIDPSLRPERGLLKIRSQLGLFANLRPALLYPQLADASTLKPEVVAGLDLLILRELTGGIYFGQPRGNRTLDNGERQAYD
TLPYSESEIRRIAKAGFEMARLRGKKLCSVDKANVLASSQLWRAVVEEVAKDYPDIALSHMYVDNAAMQLVRAPKQFDVI
VTDNMFGDILSDQASMLTGSIGMLPSASLDANSKGMYEPCHGSAPDIAGKGIANPLATILSVAMMLRYTFAQADAADAIE
RAVGKVLDQGLRTADIWSEGTTKVGTVAMGDAVVAAL

Sequences:

>Translated_357_residues
MSKQILILPGDGIGPEIMAEAVKVLKRIDAQHGLGFELVYDELGGAAYDKYGSPLADETLERARAADAVLLGAVGGPQWD
TIDPSLRPERGLLKIRSQLGLFANLRPALLYPQLADASTLKPEVVAGLDLLILRELTGGIYFGQPRGNRTLDNGERQAYD
TLPYSESEIRRIAKAGFEMARLRGKKLCSVDKANVLASSQLWRAVVEEVAKDYPDIALSHMYVDNAAMQLVRAPKQFDVI
VTDNMFGDILSDQASMLTGSIGMLPSASLDANSKGMYEPCHGSAPDIAGKGIANPLATILSVAMMLRYTFAQADAADAIE
RAVGKVLDQGLRTADIWSEGTTKVGTVAMGDAVVAAL
>Mature_356_residues
SKQILILPGDGIGPEIMAEAVKVLKRIDAQHGLGFELVYDELGGAAYDKYGSPLADETLERARAADAVLLGAVGGPQWDT
IDPSLRPERGLLKIRSQLGLFANLRPALLYPQLADASTLKPEVVAGLDLLILRELTGGIYFGQPRGNRTLDNGERQAYDT
LPYSESEIRRIAKAGFEMARLRGKKLCSVDKANVLASSQLWRAVVEEVAKDYPDIALSHMYVDNAAMQLVRAPKQFDVIV
TDNMFGDILSDQASMLTGSIGMLPSASLDANSKGMYEPCHGSAPDIAGKGIANPLATILSVAMMLRYTFAQADAADAIER
AVGKVLDQGLRTADIWSEGTTKVGTVAMGDAVVAAL

Specific function: Catalyzes the oxidation of 3-carboxy-2-hydroxy-4- methylpentanoate (3-isopropylmalate) to 3-carboxy-4-methyl-2- oxopentanoate. The product decarboxylates to 4-methyl-2 oxopentanoate [H]

COG id: COG0473

COG function: function code CE; Isocitrate/isopropylmalate dehydrogenase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the isocitrate and isopropylmalate dehydrogenases family. LeuB type 1 subfamily [H]

Homologues:

Organism=Homo sapiens, GI5031777, Length=330, Percent_Identity=32.7272727272727, Blast_Score=142, Evalue=7e-34,
Organism=Homo sapiens, GI4758582, Length=341, Percent_Identity=30.4985337243402, Blast_Score=121, Evalue=1e-27,
Organism=Homo sapiens, GI28178816, Length=362, Percent_Identity=27.6243093922652, Blast_Score=115, Evalue=7e-26,
Organism=Homo sapiens, GI28178821, Length=313, Percent_Identity=28.7539936102236, Blast_Score=113, Evalue=3e-25,
Organism=Homo sapiens, GI28178838, Length=327, Percent_Identity=29.9694189602446, Blast_Score=112, Evalue=7e-25,
Organism=Homo sapiens, GI28178819, Length=146, Percent_Identity=36.986301369863, Blast_Score=95, Evalue=8e-20,
Organism=Escherichia coli, GI87081683, Length=357, Percent_Identity=52.6610644257703, Blast_Score=370, Evalue=1e-104,
Organism=Escherichia coli, GI1788101, Length=355, Percent_Identity=35.4929577464789, Blast_Score=187, Evalue=7e-49,
Organism=Escherichia coli, GI1787381, Length=402, Percent_Identity=24.1293532338308, Blast_Score=80, Evalue=1e-16,
Organism=Caenorhabditis elegans, GI71986051, Length=346, Percent_Identity=32.6589595375723, Blast_Score=145, Evalue=3e-35,
Organism=Caenorhabditis elegans, GI17505779, Length=343, Percent_Identity=30.9037900874636, Blast_Score=128, Evalue=5e-30,
Organism=Caenorhabditis elegans, GI25144293, Length=348, Percent_Identity=30.1724137931034, Blast_Score=126, Evalue=2e-29,
Organism=Caenorhabditis elegans, GI17550882, Length=356, Percent_Identity=31.4606741573034, Blast_Score=124, Evalue=6e-29,
Organism=Saccharomyces cerevisiae, GI6319830, Length=355, Percent_Identity=50.4225352112676, Blast_Score=314, Evalue=1e-86,
Organism=Saccharomyces cerevisiae, GI6322097, Length=354, Percent_Identity=36.7231638418079, Blast_Score=184, Evalue=3e-47,
Organism=Saccharomyces cerevisiae, GI6324709, Length=353, Percent_Identity=31.4447592067989, Blast_Score=120, Evalue=4e-28,
Organism=Saccharomyces cerevisiae, GI6324291, Length=346, Percent_Identity=30.3468208092486, Blast_Score=116, Evalue=5e-27,
Organism=Drosophila melanogaster, GI24643268, Length=334, Percent_Identity=33.5329341317365, Blast_Score=150, Evalue=1e-36,
Organism=Drosophila melanogaster, GI24643270, Length=334, Percent_Identity=33.5329341317365, Blast_Score=150, Evalue=2e-36,
Organism=Drosophila melanogaster, GI24661184, Length=343, Percent_Identity=32.3615160349854, Blast_Score=131, Evalue=9e-31,
Organism=Drosophila melanogaster, GI161078633, Length=340, Percent_Identity=27.6470588235294, Blast_Score=110, Evalue=1e-24,
Organism=Drosophila melanogaster, GI24650122, Length=340, Percent_Identity=27.6470588235294, Blast_Score=110, Evalue=1e-24,
Organism=Drosophila melanogaster, GI161078637, Length=340, Percent_Identity=27.6470588235294, Blast_Score=110, Evalue=1e-24,
Organism=Drosophila melanogaster, GI161078639, Length=338, Percent_Identity=27.810650887574, Blast_Score=110, Evalue=1e-24,
Organism=Drosophila melanogaster, GI161078635, Length=340, Percent_Identity=27.6470588235294, Blast_Score=110, Evalue=1e-24,
Organism=Drosophila melanogaster, GI281362242, Length=354, Percent_Identity=29.6610169491525, Blast_Score=107, Evalue=1e-23,
Organism=Drosophila melanogaster, GI24648872, Length=354, Percent_Identity=29.6610169491525, Blast_Score=107, Evalue=1e-23,
Organism=Drosophila melanogaster, GI20130355, Length=342, Percent_Identity=26.3157894736842, Blast_Score=78, Evalue=1e-14,

Paralogues:

None

Copy number: 400 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR019818
- InterPro:   IPR001804
- InterPro:   IPR004429 [H]

Pfam domain/function: PF00180 Iso_dh [H]

EC number: =1.1.1.85 [H]

Molecular weight: Translated: 38219; Mature: 38088

Theoretical pI: Translated: 4.80; Mature: 4.80

Prosite motif: PS00470 IDH_IMDH

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
3.4 %Met     (Translated Protein)
3.9 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
3.1 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSKQILILPGDGIGPEIMAEAVKVLKRIDAQHGLGFELVYDELGGAAYDKYGSPLADETL
CCCCEEEECCCCCCHHHHHHHHHHHHHHHHHCCCCCEEHHHHHCCHHHHHCCCCHHHHHH
ERARAADAVLLGAVGGPQWDTIDPSLRPERGLLKIRSQLGLFANLRPALLYPQLADASTL
HHHHHHCEEEEECCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHCCCHHHHCCCCCCCCCC
KPEVVAGLDLLILRELTGGIYFGQPRGNRTLDNGERQAYDTLPYSESEIRRIAKAGFEMA
CHHHHHHHHHHHHHHHHCCEEECCCCCCCCCCCCCHHHHCCCCCCHHHHHHHHHHHHHHH
RLRGKKLCSVDKANVLASSQLWRAVVEEVAKDYPDIALSHMYVDNAAMQLVRAPKQFDVI
HHCCHHHCCCCHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHCCCCEEEE
VTDNMFGDILSDQASMLTGSIGMLPSASLDANSKGMYEPCHGSAPDIAGKGIANPLATIL
EECCHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHH
SVAMMLRYTFAQADAADAIERAVGKVLDQGLRTADIWSEGTTKVGTVAMGDAVVAAL
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHCCCCCCCCCEEEECCCHHHCC
>Mature Secondary Structure 
SKQILILPGDGIGPEIMAEAVKVLKRIDAQHGLGFELVYDELGGAAYDKYGSPLADETL
CCCEEEECCCCCCHHHHHHHHHHHHHHHHHCCCCCEEHHHHHCCHHHHHCCCCHHHHHH
ERARAADAVLLGAVGGPQWDTIDPSLRPERGLLKIRSQLGLFANLRPALLYPQLADASTL
HHHHHHCEEEEECCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHCCCHHHHCCCCCCCCCC
KPEVVAGLDLLILRELTGGIYFGQPRGNRTLDNGERQAYDTLPYSESEIRRIAKAGFEMA
CHHHHHHHHHHHHHHHHCCEEECCCCCCCCCCCCCHHHHCCCCCCHHHHHHHHHHHHHHH
RLRGKKLCSVDKANVLASSQLWRAVVEEVAKDYPDIALSHMYVDNAAMQLVRAPKQFDVI
HHCCHHHCCCCHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHCCCCEEEE
VTDNMFGDILSDQASMLTGSIGMLPSASLDANSKGMYEPCHGSAPDIAGKGIANPLATIL
EECCHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHH
SVAMMLRYTFAQADAADAIERAVGKVLDQGLRTADIWSEGTTKVGTVAMGDAVVAAL
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHCCCCCCCCCEEEECCCHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12024217 [H]