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The map label for this gene is ybiK [C]

Identifier: 78048778

GI number: 78048778

Start: 3672145

End: 3673209

Strand: Reverse

Name: ybiK [C]

Synonym: XCV3222

Alternate gene names: 78048778

Gene position: 3673209-3672145 (Counterclockwise)

Preceding gene: 78048782

Following gene: 78048777

Centisome position: 70.93

GC content: 64.98

Gene sequence:

>1065_bases
ATGCTTCAACGTCGTCACTTCCTCAAGAGCGCCACGCTGGGCGCGATTGCGTCCGGGTTGGCCGGCTGGGGCGCCCGCGC
GCAAGCCGCGGCAGGCGCCACGCCGGGCGCGCGGCCGACGGCGCTGCCGCGTGGAGCGGCGCGGGTGATTTCCACCTGGG
ATTTTGGCGTTGCCGCCAATCAGGAAGCCTGGAAGATTCTCAGCAGCGGCGGTCTTGCGCTGGATGCGGTGGAGGCCGGC
GTGCGAGTGCCGGAAGCGGACCCGAGCAATCCCACCGTCGGCCTGGGCGGTTATCCGGACCGCGATGGCCGGGTCACCCT
GGACGCCTGCATCATGGACCACCTGGGCAATTGCGGCGCGGTGGCATCGCTGGAAGACGTGGTGCACGCCATTTCGGTGG
CGCGTGCGGTGATGGAGAAAACGCCGCACGTGATGTTGGTAGGCGATGGTGCGCGTCAATTCGCCTTCGAACAGGGGTTT
CCGAAAACTAAGCTGTTGACCCCAAGCTCCGAAGCGGCCTGGAAAGAGTGGTTGAAGACTTCCAAGTATTCGCCAGAAGC
CAATGTCGAAAATCGTGCCTGGCGCGATGCCAAGTTGCCCGGCGGCAAGGACAATCACGACACCATCGGCATGCTGGCGC
TGGATACGCACGGCAATCTATCCGGTGCATGCACCACCAGCGGCATGGCGTGGAAAATGCACGGCCGCGTGGGCGACAGC
CCGATCATTGGTGCGGGCCTGTACGTGGACAACGAAGTGGGCGGTGCGACCTCGACTGGTGTGGGCGAGGAGGTGATCCG
CAACGTCGGCAGTTTTGCGGTGGTGGAAATGATGCGGCAGGGCAAGTCGCCGGCCGATGCCTGCCGCGAGGTGGTGATGC
GGCTGATCCGTCGCAAGCCGGAGCTGACGCGCACATTGCAGGTCGGCTTTCTGGCAATGAACAAGCGCGGCGAAGTGGGT
GCGTTCGCGATCCAGAAGGGCTTCAGTTACGCGGTGTGCGACGCGCAGCGGCAGGATCTGCTGGTGCCTGGGCAGAGCCA
TTACACCAGCGAGCCGGCGGCATGA

Upstream 100 bases:

>100_bases
ACAACACCGCAAGAAGGTTTATCCGCACGTCATGCTGTCGCATGATGGAAGGCTGGTACCGCACTGCGCGGCCGCCCGTT
TCCGTCATGGAGTCATCCGC

Downstream 100 bases:

>100_bases
GCGCGTTCGGGTTGGAGGTGGCTGCCGATTCGGTGGCCTCGGCATTGGCCGCGCAAGCAGGCGGTGCAATGCGCGTGGAG
CTGTGCGGCGGGCTGGATGG

Product: asparaginase precursor

Products: NA

Alternate protein names: Aspartylglucosaminidase; AGA; Glycosylasparaginase; N4-(N-acetyl-beta-glucosaminyl)-L-asparagine amidase [H]

Number of amino acids: Translated: 354; Mature: 354

Protein sequence:

>354_residues
MLQRRHFLKSATLGAIASGLAGWGARAQAAAGATPGARPTALPRGAARVISTWDFGVAANQEAWKILSSGGLALDAVEAG
VRVPEADPSNPTVGLGGYPDRDGRVTLDACIMDHLGNCGAVASLEDVVHAISVARAVMEKTPHVMLVGDGARQFAFEQGF
PKTKLLTPSSEAAWKEWLKTSKYSPEANVENRAWRDAKLPGGKDNHDTIGMLALDTHGNLSGACTTSGMAWKMHGRVGDS
PIIGAGLYVDNEVGGATSTGVGEEVIRNVGSFAVVEMMRQGKSPADACREVVMRLIRRKPELTRTLQVGFLAMNKRGEVG
AFAIQKGFSYAVCDAQRQDLLVPGQSHYTSEPAA

Sequences:

>Translated_354_residues
MLQRRHFLKSATLGAIASGLAGWGARAQAAAGATPGARPTALPRGAARVISTWDFGVAANQEAWKILSSGGLALDAVEAG
VRVPEADPSNPTVGLGGYPDRDGRVTLDACIMDHLGNCGAVASLEDVVHAISVARAVMEKTPHVMLVGDGARQFAFEQGF
PKTKLLTPSSEAAWKEWLKTSKYSPEANVENRAWRDAKLPGGKDNHDTIGMLALDTHGNLSGACTTSGMAWKMHGRVGDS
PIIGAGLYVDNEVGGATSTGVGEEVIRNVGSFAVVEMMRQGKSPADACREVVMRLIRRKPELTRTLQVGFLAMNKRGEVG
AFAIQKGFSYAVCDAQRQDLLVPGQSHYTSEPAA
>Mature_354_residues
MLQRRHFLKSATLGAIASGLAGWGARAQAAAGATPGARPTALPRGAARVISTWDFGVAANQEAWKILSSGGLALDAVEAG
VRVPEADPSNPTVGLGGYPDRDGRVTLDACIMDHLGNCGAVASLEDVVHAISVARAVMEKTPHVMLVGDGARQFAFEQGF
PKTKLLTPSSEAAWKEWLKTSKYSPEANVENRAWRDAKLPGGKDNHDTIGMLALDTHGNLSGACTTSGMAWKMHGRVGDS
PIIGAGLYVDNEVGGATSTGVGEEVIRNVGSFAVVEMMRQGKSPADACREVVMRLIRRKPELTRTLQVGFLAMNKRGEVG
AFAIQKGFSYAVCDAQRQDLLVPGQSHYTSEPAA

Specific function: Cleaves the GlcNAc-Asn bond which joins oligosaccharides to the peptide of asparagine-linked glycoproteins. Requires that the glycosylated asparagine moiety is not substituted on its N- (R1) and C- (R2) terminus [H]

COG id: COG1446

COG function: function code E; Asparaginase

Gene ontology:

Cell location: Periplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the Ntn-hydrolase family [H]

Homologues:

Organism=Homo sapiens, GI285002251, Length=319, Percent_Identity=36.6771159874608, Blast_Score=169, Evalue=3e-42,
Organism=Homo sapiens, GI285002253, Length=319, Percent_Identity=34.7962382445141, Blast_Score=146, Evalue=2e-35,
Organism=Homo sapiens, GI145275202, Length=229, Percent_Identity=37.5545851528384, Blast_Score=125, Evalue=9e-29,
Organism=Homo sapiens, GI145275200, Length=229, Percent_Identity=37.5545851528384, Blast_Score=125, Evalue=9e-29,
Organism=Homo sapiens, GI98991769, Length=235, Percent_Identity=28.936170212766, Blast_Score=81, Evalue=1e-15,
Organism=Escherichia coli, GI1787050, Length=259, Percent_Identity=37.0656370656371, Blast_Score=136, Evalue=2e-33,
Organism=Caenorhabditis elegans, GI17569239, Length=302, Percent_Identity=34.7682119205298, Blast_Score=139, Evalue=3e-33,
Organism=Drosophila melanogaster, GI221330102, Length=301, Percent_Identity=40.531561461794, Blast_Score=184, Evalue=9e-47,
Organism=Drosophila melanogaster, GI24652135, Length=301, Percent_Identity=40.531561461794, Blast_Score=183, Evalue=2e-46,
Organism=Drosophila melanogaster, GI24655553, Length=300, Percent_Identity=34.6666666666667, Blast_Score=155, Evalue=4e-38,
Organism=Drosophila melanogaster, GI24657358, Length=289, Percent_Identity=33.5640138408304, Blast_Score=134, Evalue=8e-32,
Organism=Drosophila melanogaster, GI18921183, Length=281, Percent_Identity=34.5195729537367, Blast_Score=117, Evalue=1e-26,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000246
- InterPro:   IPR006311 [H]

Pfam domain/function: PF01112 Asparaginase_2 [H]

EC number: =3.5.1.26 [H]

Molecular weight: Translated: 37377; Mature: 37377

Theoretical pI: Translated: 8.23; Mature: 8.23

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
4.5 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
3.1 %Met     (Mature Protein)
4.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLQRRHFLKSATLGAIASGLAGWGARAQAAAGATPGARPTALPRGAARVISTWDFGVAAN
CCCHHHHHHHHHHHHHHHHHHCCCCCHHHHCCCCCCCCCCCCCCCHHHHHECCCCCCCCC
QEAWKILSSGGLALDAVEAGVRVPEADPSNPTVGLGGYPDRDGRVTLDACIMDHLGNCGA
HHHHHHHHCCCCEEEHHHHCCCCCCCCCCCCEEECCCCCCCCCCEEHHHHHHHHCCCCCC
VASLEDVVHAISVARAVMEKTPHVMLVGDGARQFAFEQGFPKTKLLTPSSEAAWKEWLKT
HHHHHHHHHHHHHHHHHHHCCCCEEEEECCHHHHHHHCCCCCCEEECCCCHHHHHHHHHH
SKYSPEANVENRAWRDAKLPGGKDNHDTIGMLALDTHGNLSGACTTSGMAWKMHGRVGDS
CCCCCCCCCCCCCCCCCCCCCCCCCCCEEEEEEEECCCCCCCEEECCCEEEEECCCCCCC
PIIGAGLYVDNEVGGATSTGVGEEVIRNVGSFAVVEMMRQGKSPADACREVVMRLIRRKP
CEEEEEEEEECCCCCCCCCCCHHHHHHHHCHHHHHHHHHCCCCHHHHHHHHHHHHHHCCC
ELTRTLQVGFLAMNKRGEVGAFAIQKGFSYAVCDAQRQDLLVPGQSHYTSEPAA
HHHHHHHHHEEEECCCCCCCHHHHHCCCCEEEECCCCCCEECCCCCCCCCCCCC
>Mature Secondary Structure
MLQRRHFLKSATLGAIASGLAGWGARAQAAAGATPGARPTALPRGAARVISTWDFGVAAN
CCCHHHHHHHHHHHHHHHHHHCCCCCHHHHCCCCCCCCCCCCCCCHHHHHECCCCCCCCC
QEAWKILSSGGLALDAVEAGVRVPEADPSNPTVGLGGYPDRDGRVTLDACIMDHLGNCGA
HHHHHHHHCCCCEEEHHHHCCCCCCCCCCCCEEECCCCCCCCCCEEHHHHHHHHCCCCCC
VASLEDVVHAISVARAVMEKTPHVMLVGDGARQFAFEQGFPKTKLLTPSSEAAWKEWLKT
HHHHHHHHHHHHHHHHHHHCCCCEEEEECCHHHHHHHCCCCCCEEECCCCHHHHHHHHHH
SKYSPEANVENRAWRDAKLPGGKDNHDTIGMLALDTHGNLSGACTTSGMAWKMHGRVGDS
CCCCCCCCCCCCCCCCCCCCCCCCCCCEEEEEEEECCCCCCCEEECCCEEEEECCCCCCC
PIIGAGLYVDNEVGGATSTGVGEEVIRNVGSFAVVEMMRQGKSPADACREVVMRLIRRKP
CEEEEEEEEECCCCCCCCCCCHHHHHHHHCHHHHHHHHHCCCCHHHHHHHHHHHHHHCCC
ELTRTLQVGFLAMNKRGEVGAFAIQKGFSYAVCDAQRQDLLVPGQSHYTSEPAA
HHHHHHHHHEEEECCCCCCCHHHHHCCCCEEEECCCCCCEECCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7840643; 8250923; 9541410 [H]