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The map label for this gene is gcvH [H]

Identifier: 78048751

GI number: 78048751

Start: 3633650

End: 3634045

Strand: Reverse

Name: gcvH [H]

Synonym: XCV3195

Alternate gene names: 78048751

Gene position: 3634045-3633650 (Counterclockwise)

Preceding gene: 78048752

Following gene: 78048750

Centisome position: 70.18

GC content: 62.88

Gene sequence:

>396_bases
ATGAGCGAGATCCCTGGCGACCTCAAGTTCCTCAAATCCCACGAGTGGGCCCGCGTCGAAAGCGACGGTCGCGTGACCGT
CGGCATTTCCGACCACGCGCAGGGCTTGCTGGGTGACCTGGTTTACGTCGAACTGCCGGGCGTCGGCGACACCGTGCAGG
TCGGCAACGGCGCTGCGGTGGTGGAATCGGTCAAGGCCGCCTCCGACGTCTACAGCCCGGTCAGCGGCACCGTTGTCGAG
GTCAACAGCGCGTTGAGCGACAAGCCGGAAACCATCAACGAAGACGCCTATGGCGAAGGCTGGATCTTCGTCGTCGAGAT
CGACGACAAGGAACAGCTCAACGACCTGCTGGACCCGGACGACTATGCCGAGCTGCTCGAAGACGACGATCACTGA

Upstream 100 bases:

>100_bases
CCGTATCGCCATCGCCCCACCGCCGTGCGTGCACCTGTCTTGTCGCGCGGTCTAAACTAGCCACCTGTCCCCCAACCATC
ATCTTTCCCGGAGCACACCC

Downstream 100 bases:

>100_bases
TCAGGTGTCGTTGCAGCCGGTATCCGATGGCCGCTCATGGAGCGGCCATTTTTTTTGAGCGCGCCTGCGTCGGTGATGCG
TGCCTTGGTGCAGCGATGCG

Product: glycine cleavage system protein H

Products: Proton; NADH; NH3; CO2; 5,10-methylene-THF [C]

Alternate protein names: NA

Number of amino acids: Translated: 131; Mature: 130

Protein sequence:

>131_residues
MSEIPGDLKFLKSHEWARVESDGRVTVGISDHAQGLLGDLVYVELPGVGDTVQVGNGAAVVESVKAASDVYSPVSGTVVE
VNSALSDKPETINEDAYGEGWIFVVEIDDKEQLNDLLDPDDYAELLEDDDH

Sequences:

>Translated_131_residues
MSEIPGDLKFLKSHEWARVESDGRVTVGISDHAQGLLGDLVYVELPGVGDTVQVGNGAAVVESVKAASDVYSPVSGTVVE
VNSALSDKPETINEDAYGEGWIFVVEIDDKEQLNDLLDPDDYAELLEDDDH
>Mature_130_residues
SEIPGDLKFLKSHEWARVESDGRVTVGISDHAQGLLGDLVYVELPGVGDTVQVGNGAAVVESVKAASDVYSPVSGTVVEV
NSALSDKPETINEDAYGEGWIFVVEIDDKEQLNDLLDPDDYAELLEDDDH

Specific function: The glycine cleavage system catalyzes the degradation of glycine. The H protein shuttles the methylamine group of glycine from the P protein to the T protein [H]

COG id: COG0509

COG function: function code E; Glycine cleavage system H protein (lipoate-binding)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 lipoyl-binding domain [H]

Homologues:

Organism=Homo sapiens, GI49574537, Length=120, Percent_Identity=40, Blast_Score=99, Evalue=1e-21,
Organism=Homo sapiens, GI89057342, Length=120, Percent_Identity=39.1666666666667, Blast_Score=96, Evalue=1e-20,
Organism=Escherichia coli, GI1789271, Length=129, Percent_Identity=56.5891472868217, Blast_Score=152, Evalue=8e-39,
Organism=Caenorhabditis elegans, GI17551294, Length=122, Percent_Identity=44.2622950819672, Blast_Score=106, Evalue=4e-24,
Organism=Caenorhabditis elegans, GI17507493, Length=121, Percent_Identity=40.495867768595, Blast_Score=102, Evalue=6e-23,
Organism=Saccharomyces cerevisiae, GI6319272, Length=132, Percent_Identity=37.8787878787879, Blast_Score=97, Evalue=9e-22,
Organism=Drosophila melanogaster, GI17865652, Length=131, Percent_Identity=38.9312977099237, Blast_Score=102, Evalue=7e-23,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003016
- InterPro:   IPR002930
- InterPro:   IPR017453
- InterPro:   IPR011053 [H]

Pfam domain/function: PF01597 GCV_H [H]

EC number: NA

Molecular weight: Translated: 14102; Mature: 13971

Theoretical pI: Translated: 3.71; Mature: 3.71

Prosite motif: PS00189 LIPOYL

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
0.8 %Met     (Translated Protein)
0.8 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
0.0 %Met     (Mature Protein)
0.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSEIPGDLKFLKSHEWARVESDGRVTVGISDHAQGLLGDLVYVELPGVGDTVQVGNGAAV
CCCCCCHHHHHHCCCCEEECCCCEEEEEECCCCHHHHCCEEEEEECCCCCEEEECCCHHH
VESVKAASDVYSPVSGTVVEVNSALSDKPETINEDAYGEGWIFVVEIDDKEQLNDLLDPD
HHHHHHHHHHCCCCCCEEEEECCCCCCCCCCCCCCCCCCCEEEEEEECCHHHHHHCCCCC
DYAELLEDDDH
HHHHHHCCCCC
>Mature Secondary Structure 
SEIPGDLKFLKSHEWARVESDGRVTVGISDHAQGLLGDLVYVELPGVGDTVQVGNGAAV
CCCCCHHHHHHCCCCEEECCCCEEEEEECCCCHHHHCCEEEEEECCCCCEEEECCCHHH
VESVKAASDVYSPVSGTVVEVNSALSDKPETINEDAYGEGWIFVVEIDDKEQLNDLLDPD
HHHHHHHHHHCCCCCCEEEEECCCCCCCCCCCCCCCCCCCEEEEEEECCHHHHHHCCCCC
DYAELLEDDDH
HHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: Lipoyl Cofactor. [C]

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NAD; L-glycine; THF [C]

Specific reaction: NAD + L-glycine + THF = Proton + NADH + NH3 + CO2 + 5,10-methylene-THF [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12024217 [H]